Progress and Prospect of Applications of Silk Fibroin in Construction of Tissue Engineering Scaffold_Journal of Biomedical Engineering

Authors：

YINLihua , WANGLin ,  YUZhanhai

Stomatological College of Lanzhou University, Lanzhou 730000, China;

Corresponding?author：

YUZhanhai, Email: yuzhanhai@lzu.edu.cn

Keywords：

silk fibroin; scaffold construction; tissue engineering

DOI：

10.7507/1001-5515.20140086

Video：

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With the development of tissue engineering, a variety of forms of silk fibroin (SF) scaffolds has been applied to research of constructing variety of organization based on cells, which has become scientific focus in recent years. In this paper we introduced the source and structure of SF and the fabrication method of the scaffold, and also address the SF application progress in several relevant fields of tissue engineering, such as bone, cartilage, skin, blood vessel and nerves. Finally, we discuss the future leading prospect of the SF in order to provide reference for subsequent research.

Citation： YINLihua, WANGLin, YUZhanhai. Progress and Prospect of Applications of Silk Fibroin in Construction of Tissue Engineering Scaffold. Journal of Biomedical Engineering, 2014, 31(2): 467-471. doi: 10.7507/1001-5515.20140086 Copy

1.	LANGER R,VACANTI J P.Tissue engineering[J].Science,1993,260(5110):920-926.
2.	吳任東,楊輝,張磊,等.組織工程支架快速成形技術研究現狀[J].機械工程學報,2011,47(5):170-176.
3.	OMENETTO F G,KAPLAN D L.New opportunities for an ancient material[J].Science,2010,329(5991):528-531.
4.	BITTENCOURT D,OLIVEIRA P F,PROSDOCIMI F,et al.Protein families,natural history and biotechnological aspects of spider silk[J].Genet Mol Res,2012,11(3):2360-2380.
5.	VEPARI C,KAPLAN D L.Silk as a biomaterial[J].Prog Polym Sci,2007,32(8-9):991-1007.
6.	WANG Y,KIM H J,VUNJAK-NOVAKOVIC G,et al.Stem cell-based tissue engineering with silk biomaterials[J].Biomaterials,2006,27(36):6064-6082.
7.	胡燁婷,王新剛,俞曉波,等.組織工程支架材料絲素蛋白研究進展[J].中華燒傷雜志,2011,27(4):291-294.
8.	GIL E S,PARK S H,MARCHANT J,et al.Response of human corneal fibroblasts on silk film surface patterns[J].Macromol Biosci,2010,10(6):664-673.
9.	ZHANG K H,YIN A L,HUANG C,et al.Degradation of electrospun SF/P (LLA-CL) blended nanofibrous scaffolds in vitro[J].Polym Degrad Stabil,2011,96(12):2266-2275.
10.	KUNDU S C,KUNDU B,TALUKDAR S,et al.Invited review nonmulberry silk biopolymers[J].Biopolymers,2012,97(6):455-467.
11.	LAWRENCE B D,WHARRAM S,KLUGE J A,et al.Effect of hydration on silk film material properties[J].Macromol Biosci,2010,10(4):393-403.
12.	CHI N H,YANG M C,CHUNG T W,et al.Cardiac repair achieved by bone marrow mesenchymal stem cells/silk fibroin/hyaluronic acid patches in a rat of myocardial infarction model[J].Biomaterials,2012,33(22):5541-5551.
13.	JHA B S,COLELLO R J,BOWMAN J R,et al.Two pole air gap electrospinning:fabrication of highly aligned,three-dimensional scaffolds for nerve reconstruction[J].Acta Biomater,2011,7(1):203-215.
14.	LI S,WU H,HU X D,et al.Preparation of electrospun PLGA-silk fibroin nanofibers-based nerve conduits and evaluation in vivo[J].Artif Cells Blood Substit Immobil Biotechnol,2012,40(1-2):171-178.
15.	CHO Y I,CHOI J S,JEONG S Y,et al.Nerve growth factor (NGF)-conjugated electrospun nanostructures with topographical cues for neuronal differentiation of mesenchymal stem cells[J].Acta Biomater,2010,6(12):4725-4733.
16.	LAWRENCE B D,MARCHANT J K,PINDRUS M A,et al.Silk film biomaterials for cornea tissue engineering[J].Biomaterials,2009,30(7):1299-1308.
17.	GUZIEWICZ N,BEST A,PEREZ-RAMIREZ B,et al.Lyophilized silk fibroinhydrogels for the sustained local delivery of therapeutic monoclonal antibodies[J].Biomaterials,2011,32(10):2642-2650.
18.	CHAO P H,YODMUANG S,WANG X,et al.Silk hydrogel for cartilage tissue engineering[J].J Biomed Mater Res B Appl Biomater,2010,95(1):84-90.
19.	DIAB T,PRITCHARD E M,UHRIG B A,et al.A silk hydrogel-based delivery system of bone morphogenetic protein for the treatment of large bone defects[J].J Mech Behav Biomed Mater,2012,11(11):123-131.
20.	HOFMANN S,HAGENMVLLER H,KOCH A M,et al.Control of in vitro tissue-engineered bone-like structures using human mesenchymal stem cells and porous silk scaffolds[J].Biomaterials,2007,28(6):1152-1162.
21.	MANDAL B B,GRINBERG A,GIL E S,et al.High-strength silk protein scaffolds for bone repair[J].Proc Natl Acad Sci U S A,2012,109(20):7699-7704.
22.	KINAHAN M E,FILIPPIDI E,K?STER S,et al.Tunable silk:using microfluidics to fabricate silk fibers with controllable properties[J].Biomacromolecules,2011,12(5):1504-1511.
23.	ZHAO Y,CHEN J,CHOU A H,et al.Nonwoven silk fibroin net/nano-hydroxyapatite scaffold:preparation and characterization[J].J Biomed Mater Res a,2009,91(4):1140-1149.
24.	WANG J,YE R,WEI Y,et al.The effects of electrospun TSF nanofiber diameter and alignment on neuronal differentiation of human embryonic stem cells[J].J Biomed Mater Res A,2012,100(3):632-645.
25.	江霞蓉,管娟,陳新,等.具有定向形貌的絲素蛋白支架的制備[J].化學學報,2010,68(18):1909-1914.
26.	CHO H J,KI C S,OH H,et al.Molecular weight distribution and solution properties of silk fibroins with different dissolution conditions[J].Int J Biol Macromol,2012,51(3):336-341.
27.	YANG M,SHUAI Y,HE W,et al.Preparation of porous scaffolds from silk fibroin extracted from the silk gland of bombyx mori (B.mori)[J].Int J Mol Sci,2012,13(6):7762-7775.
28.	RAJKHOWA R,GIL E S,KLUGE J,et al.Reinforcing silk scaffolds with silk particles[J].Macromol Biosci,2010,10(6):599-611.
29.	楊磊,朱良均,楊明英,等.絲素組織工程支架的研究進展[J].蠶業科學,2011,37(2):296-302.
30.	FISCHER R L,MCCOY M G,GRANT S A.Electrospinning collagen and hyaluronic acid nanofiber meshes[J].J Mater Sci Mater Med,2012,(7):1645-1654.
31.	ZHANG F,ZUO B Q,FAN Z H,et al.Mechanisms and control of silk-based electrospinning[J].Biomacromolecules,2012,13(3):798-804.
32.	ZHOU J,CAO C B,MA X L,et al.Electrospinning of silk fibroin and collagen for vascular tissue engineering[J].Int J Biol Macromol,2010,47(4):514-519.
33.	黃繼偉,張鋒,左保齊.靜電紡絲素蛋白及其應用于組織工程的研究進展[J].天津工業大學學報,2011,30(1):16-21.
34.	吳小建,陳一心.蠶絲絲素蛋白在骨科領域的應用進展[J].臨床骨科雜志,2011,14(2):214-217.
35.	MEINEL L,FAJARDO R,HOFMANN S,et al.Silk implants for the healing of critical size bone defects[J].Bone,2005,37(5):688-698.
36.	PARK S Y,KI C S,PARK Y H,et al.Electrospun silk fibroin scaffolds with macropores for bone regeneration:an in vitro and in vivo study[J].Tissue Eng Part A,2010,16(4):1271-1279.
37.	ROCKWOOD D N,GIL E S,PARK S H,et al.Ingrowth of human mesenchymal stem cells into porous silk particle reinforced silk composite scaffolds:an in vitro study[J].Acta Biomater,2011,7(1):144-151.
38.	CORREIA C,BHUMIRATANA S,YAN L P,et al.Development of silk-based scaffolds for tissue engineering of bone from human adipose-derived stem cells[J].Acta Biomater,2012,8(7):2483-2492.
39.	CHEON Y W,LEE W J,BAEK H S,et al.Enhanced chondrogenic responses of human articular chondrocytes onto silk fibroin/wool keratose scaffolds treated with microwave-induced argon plasma[J].Artif Organs,2010,34(5):384-392.
40.	KAMBE Y,YAMAMOTO K,KOJIMA K,et al.Effects of RGDS sequence genetically interfused in the silk fibroin light chain protein on chondrocyte adhesion and cartilage synthesis[J].Biomaterials,2010,31(29):7503-7511.
41.	JEONG L,YEO I S,KIM H N,et al.Plasma-treated silk fibroin nanofibers for skin regeneration[J].Int J Biol Macromol,2009,44(3):222-228.
42.	GUAN G,BAI L,ZUO B,et al.Promoted dermis healing from full-thickness skin defect by porous silk fibroin scaffolds (PSFSs)[J].Biomed Mater Eng,2010,20(5):295-308.
43.	BHARDWAJ N,CHAKRABORTY S,KUNDU S C.Freeze-gelled silk fibroin protein scaffolds for potential applications in soft tissue engineering[J].Int J Biol Macromol,2011,49(3):260-267.
44.	侯春春,張胡靜,李圣春,等.蠶絲蛋白生物醫學材料的研究現狀[J].絲綢,2010,33(7):18-22.
45.	ZHANG K H,MO X M,HUANG C,et al.Electrospun scaffolds from silk fibroin and their cellular compatibility[J].J Biomed Mater Res a,2010,93(3):976-983.
46.	SOFFER L,WANG X Y,ZHANG X H,et al.Silk-based electrospun tubular scaffolds for tissue-engineered vascular grafts[J].J Biomater Sci Polym Ed,2008,19(5):653-664.
47.	TANG X,DING F,YANG Y,et al.Evaluation on in vitro biocompatibility of silk fibroin-based biomaterials with primarily cultured hippocampal neurons[J].J Biomed Mater Res A,2009,91(1):166-174.
48.	TANG X,XUE C,WANG Y,et al.Bridging peripheral nerve defects with a tissue engineered nerve graft composed of an in vitro cultured nerve equivalent and a silk fibroin-based scaffold[J].Biomaterials,2012,33(15):3860-3867.(上接第461頁;Continued from Page 461)顱內感染患者顱內壓的臨床應用價值[J].臨床神經病學雜志,2012,25(3):177-180.

1. LANGER R,VACANTI J P.Tissue engineering[J].Science,1993,260(5110):920-926.
2. 吳任東,楊輝,張磊,等.組織工程支架快速成形技術研究現狀[J].機械工程學報,2011,47(5):170-176.
3. OMENETTO F G,KAPLAN D L.New opportunities for an ancient material[J].Science,2010,329(5991):528-531.
4. BITTENCOURT D,OLIVEIRA P F,PROSDOCIMI F,et al.Protein families,natural history and biotechnological aspects of spider silk[J].Genet Mol Res,2012,11(3):2360-2380.
5. VEPARI C,KAPLAN D L.Silk as a biomaterial[J].Prog Polym Sci,2007,32(8-9):991-1007.
6. WANG Y,KIM H J,VUNJAK-NOVAKOVIC G,et al.Stem cell-based tissue engineering with silk biomaterials[J].Biomaterials,2006,27(36):6064-6082.
7. 胡燁婷,王新剛,俞曉波,等.組織工程支架材料絲素蛋白研究進展[J].中華燒傷雜志,2011,27(4):291-294.
8. GIL E S,PARK S H,MARCHANT J,et al.Response of human corneal fibroblasts on silk film surface patterns[J].Macromol Biosci,2010,10(6):664-673.
9. ZHANG K H,YIN A L,HUANG C,et al.Degradation of electrospun SF/P (LLA-CL) blended nanofibrous scaffolds in vitro[J].Polym Degrad Stabil,2011,96(12):2266-2275.
10. KUNDU S C,KUNDU B,TALUKDAR S,et al.Invited review nonmulberry silk biopolymers[J].Biopolymers,2012,97(6):455-467.
11. LAWRENCE B D,WHARRAM S,KLUGE J A,et al.Effect of hydration on silk film material properties[J].Macromol Biosci,2010,10(4):393-403.
12. CHI N H,YANG M C,CHUNG T W,et al.Cardiac repair achieved by bone marrow mesenchymal stem cells/silk fibroin/hyaluronic acid patches in a rat of myocardial infarction model[J].Biomaterials,2012,33(22):5541-5551.
13. JHA B S,COLELLO R J,BOWMAN J R,et al.Two pole air gap electrospinning:fabrication of highly aligned,three-dimensional scaffolds for nerve reconstruction[J].Acta Biomater,2011,7(1):203-215.
14. LI S,WU H,HU X D,et al.Preparation of electrospun PLGA-silk fibroin nanofibers-based nerve conduits and evaluation in vivo[J].Artif Cells Blood Substit Immobil Biotechnol,2012,40(1-2):171-178.
15. CHO Y I,CHOI J S,JEONG S Y,et al.Nerve growth factor (NGF)-conjugated electrospun nanostructures with topographical cues for neuronal differentiation of mesenchymal stem cells[J].Acta Biomater,2010,6(12):4725-4733.
16. LAWRENCE B D,MARCHANT J K,PINDRUS M A,et al.Silk film biomaterials for cornea tissue engineering[J].Biomaterials,2009,30(7):1299-1308.
17. GUZIEWICZ N,BEST A,PEREZ-RAMIREZ B,et al.Lyophilized silk fibroinhydrogels for the sustained local delivery of therapeutic monoclonal antibodies[J].Biomaterials,2011,32(10):2642-2650.
18. CHAO P H,YODMUANG S,WANG X,et al.Silk hydrogel for cartilage tissue engineering[J].J Biomed Mater Res B Appl Biomater,2010,95(1):84-90.
19. DIAB T,PRITCHARD E M,UHRIG B A,et al.A silk hydrogel-based delivery system of bone morphogenetic protein for the treatment of large bone defects[J].J Mech Behav Biomed Mater,2012,11(11):123-131.
20. HOFMANN S,HAGENMVLLER H,KOCH A M,et al.Control of in vitro tissue-engineered bone-like structures using human mesenchymal stem cells and porous silk scaffolds[J].Biomaterials,2007,28(6):1152-1162.
21. MANDAL B B,GRINBERG A,GIL E S,et al.High-strength silk protein scaffolds for bone repair[J].Proc Natl Acad Sci U S A,2012,109(20):7699-7704.
22. KINAHAN M E,FILIPPIDI E,K?STER S,et al.Tunable silk:using microfluidics to fabricate silk fibers with controllable properties[J].Biomacromolecules,2011,12(5):1504-1511.
23. ZHAO Y,CHEN J,CHOU A H,et al.Nonwoven silk fibroin net/nano-hydroxyapatite scaffold:preparation and characterization[J].J Biomed Mater Res a,2009,91(4):1140-1149.
24. WANG J,YE R,WEI Y,et al.The effects of electrospun TSF nanofiber diameter and alignment on neuronal differentiation of human embryonic stem cells[J].J Biomed Mater Res A,2012,100(3):632-645.
25. 江霞蓉,管娟,陳新,等.具有定向形貌的絲素蛋白支架的制備[J].化學學報,2010,68(18):1909-1914.
26. CHO H J,KI C S,OH H,et al.Molecular weight distribution and solution properties of silk fibroins with different dissolution conditions[J].Int J Biol Macromol,2012,51(3):336-341.
27. YANG M,SHUAI Y,HE W,et al.Preparation of porous scaffolds from silk fibroin extracted from the silk gland of bombyx mori (B.mori)[J].Int J Mol Sci,2012,13(6):7762-7775.
28. RAJKHOWA R,GIL E S,KLUGE J,et al.Reinforcing silk scaffolds with silk particles[J].Macromol Biosci,2010,10(6):599-611.
29. 楊磊,朱良均,楊明英,等.絲素組織工程支架的研究進展[J].蠶業科學,2011,37(2):296-302.
30. FISCHER R L,MCCOY M G,GRANT S A.Electrospinning collagen and hyaluronic acid nanofiber meshes[J].J Mater Sci Mater Med,2012,(7):1645-1654.
31. ZHANG F,ZUO B Q,FAN Z H,et al.Mechanisms and control of silk-based electrospinning[J].Biomacromolecules,2012,13(3):798-804.
32. ZHOU J,CAO C B,MA X L,et al.Electrospinning of silk fibroin and collagen for vascular tissue engineering[J].Int J Biol Macromol,2010,47(4):514-519.
33. 黃繼偉,張鋒,左保齊.靜電紡絲素蛋白及其應用于組織工程的研究進展[J].天津工業大學學報,2011,30(1):16-21.
34. 吳小建,陳一心.蠶絲絲素蛋白在骨科領域的應用進展[J].臨床骨科雜志,2011,14(2):214-217.
35. MEINEL L,FAJARDO R,HOFMANN S,et al.Silk implants for the healing of critical size bone defects[J].Bone,2005,37(5):688-698.
36. PARK S Y,KI C S,PARK Y H,et al.Electrospun silk fibroin scaffolds with macropores for bone regeneration:an in vitro and in vivo study[J].Tissue Eng Part A,2010,16(4):1271-1279.
37. ROCKWOOD D N,GIL E S,PARK S H,et al.Ingrowth of human mesenchymal stem cells into porous silk particle reinforced silk composite scaffolds:an in vitro study[J].Acta Biomater,2011,7(1):144-151.
38. CORREIA C,BHUMIRATANA S,YAN L P,et al.Development of silk-based scaffolds for tissue engineering of bone from human adipose-derived stem cells[J].Acta Biomater,2012,8(7):2483-2492.
39. CHEON Y W,LEE W J,BAEK H S,et al.Enhanced chondrogenic responses of human articular chondrocytes onto silk fibroin/wool keratose scaffolds treated with microwave-induced argon plasma[J].Artif Organs,2010,34(5):384-392.
40. KAMBE Y,YAMAMOTO K,KOJIMA K,et al.Effects of RGDS sequence genetically interfused in the silk fibroin light chain protein on chondrocyte adhesion and cartilage synthesis[J].Biomaterials,2010,31(29):7503-7511.
41. JEONG L,YEO I S,KIM H N,et al.Plasma-treated silk fibroin nanofibers for skin regeneration[J].Int J Biol Macromol,2009,44(3):222-228.
42. GUAN G,BAI L,ZUO B,et al.Promoted dermis healing from full-thickness skin defect by porous silk fibroin scaffolds (PSFSs)[J].Biomed Mater Eng,2010,20(5):295-308.
43. BHARDWAJ N,CHAKRABORTY S,KUNDU S C.Freeze-gelled silk fibroin protein scaffolds for potential applications in soft tissue engineering[J].Int J Biol Macromol,2011,49(3):260-267.
44. 侯春春,張胡靜,李圣春,等.蠶絲蛋白生物醫學材料的研究現狀[J].絲綢,2010,33(7):18-22.
45. ZHANG K H,MO X M,HUANG C,et al.Electrospun scaffolds from silk fibroin and their cellular compatibility[J].J Biomed Mater Res a,2010,93(3):976-983.
46. SOFFER L,WANG X Y,ZHANG X H,et al.Silk-based electrospun tubular scaffolds for tissue-engineered vascular grafts[J].J Biomater Sci Polym Ed,2008,19(5):653-664.
47. TANG X,DING F,YANG Y,et al.Evaluation on in vitro biocompatibility of silk fibroin-based biomaterials with primarily cultured hippocampal neurons[J].J Biomed Mater Res A,2009,91(1):166-174.
48. TANG X,XUE C,WANG Y,et al.Bridging peripheral nerve defects with a tissue engineered nerve graft composed of an in vitro cultured nerve equivalent and a silk fibroin-based scaffold[J].Biomaterials,2012,33(15):3860-3867.(上接第461頁;Continued from Page 461)顱內感染患者顱內壓的臨床應用價值[J].臨床神經病學雜志,2012,25(3):177-180.

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