Biocompatibility evaluation of electrospun PLCL/fibrinogen nanofibers in anterior cruciate ligament reconstruction_Journal of Biomedical Engineering

Authors：

GUO Jiahua ^1,2 , ZHANG Yu ² , CHEN Liyuan ² , XU Liming ² ,  MO Xiumei ¹ ,  CHEN Liang ²

1. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P. R. China;
2. National Institutes for Food and Drug Control, Beijing 102629, P. R. China;

Corresponding?author：

MO Xiumei, Email: xmm@dhu.edu.cn; CHEN Liang, Email: chenliang@nifdc.org.cn

Keywords：

Biocompatibility; Electrospun; PLCL/Fibrinogen nanofibers; Ligament reconstruction

DOI：

10.7507/1001-5515.202107011

Video：

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The study aimed to evaluate the safety and function of poly(lactic-acid-co-ε-caprolactone) (PLCL)/fibrinogen nanofibers (P/F-Ns), and provide theoretical basis for the clinical application. The surface morphology, mechanical properties, the hydrophilicity and the fibrinogen content of P/F-Ns were tested by scanning electron microscope, the material testing machine, the contact angle meter and the microplate reader, respectively. The cell adhesion, proliferation and ligament remodeling genes expression of Hig-82 cells on P/F-Ns were conducted through cell counting kit-8 (CCK-8) and real-time quantitative PCR analyses, respectively. The results showed that with the increase of the fibrinogen content, the pore sizes and hydrophilicity of three P/F-Ns increased, but the mechanical properties decreased. Cell adhesion and proliferation tests showed that P/F-N-2 held the best ability to promote cell adhesion and proliferation. The ligament remodeling genes expressions of Hig-82 cells on P/F-N-1, P/F-N-2 and P/F-N-3 were all up-regulated compared to P/F-N-0 on days 3 and 7. All the three P/F-Ns containing fibrinogen (P/F-N-1, P/F-N-2 and P/F-N-3) had better biocompatibility compared to P/F-N-0, and could be efficiently applied to the reconstruction of anterior cruciate ligament.

Citation： GUO Jiahua, ZHANG Yu, CHEN Liyuan, XU Liming, MO Xiumei, CHEN Liang. Biocompatibility evaluation of electrospun PLCL/fibrinogen nanofibers in anterior cruciate ligament reconstruction. Journal of Biomedical Engineering, 2022, 39(3): 544-550, 560. doi: 10.7507/1001-5515.202107011 Copy

1.	Chen P, Zhu S, Wang Y, et al. The amelioration of cartilage degeneration by ADAMTS-5 inhibitor delivered in a hyaluronic acid hydrogel. Biomaterials, 2014, 35(9): 2827-2836.
2.	Kim H S, Seon J K, Jo A R. Current trends in anterior cruciate ligament reconstruction. Knee Surg Relat Res, 2013, 25: 165-173.
3.	Cohen M, Amaro J T, Ejnisman B, et al. Anterior cruciate ligament reconstruction after 10 to 15 years: association between meniscectomy and osteoarthrosis. Arthroscopy, 2007, 23: 629-634.
4.	Buoncristiani A M, Tjoumakaris F P, Starman J S, et al. Anatomic double-bundle anterior cruciate ligament reconstruction. Arthroscopy, 2006, 22: 11-16.
5.	Brunet P, Charrois O, Degeorges R, et a1. Reconstruction of acute posterior cruciate ligament tears usinga synthetic ligament. Rev Chir Orthop Reparatrice Appar Mot, 2005, 91(1): 34-43.
6.	余浩, 鄧晚秋, 桑鵬, 劉毅. 關節鏡下自體同側腓骨長肌腱重建膝關節前交叉韌帶. 中國修復重建外科雜志, 2020, 34(7): 843-847.
7.	Schindler O S. Surgery for anterior cruciate ligament deficiency: a historical perspective. Knee Surg Sports Traumatol Arthrosc, 2012, 20: 5-7.
8.	Seo Y K, Yoon H H, Song K Y, et al. Increase in cell migration and angiogenesis in a composite silk scaffold for tissue-engineered ligaments. J Orthop Res, 2009, 27: 495-503.
9.	Schindler O S. Surgery for anterior cruciate ligament deficiency: a historical perspective. Knee Surg Sports Traumatol Arthrosc, 2012, 20: 45-47.
10.	Murray A W, Macnicol M F. 10-16 years results of Leeds-Keio anterior cruciate ligament reconstruction. Knee, 2004, 11: 9-14.
11.	Indelicato P A, Pascale M S, Huegel M O. Early experience with the GORE-TEX polytetrafluoroethylene anterior cruciate ligament prosthesis. Am J Sports Med, 1989, 17: 52-55.
12.	Woods G A, Indelicato P A, Prevot T J. The Gore-Tex anterior cruciate ligament prosthesis. Two versus three year results. Am J Sports Med, 1991, 19: 48-55.
13.	Maletius W, Gillquist J. Long-term results of anterior cruciate ligament reconstruction with a Dacron prosthesis. The frequency of osteoarthritis after seven to eleven years. Am J Sports Med, 1997, 25: 88-93.
14.	Rushton N, Dandy D J, Naylor C P. The clinical, arthroscopic and histological findings after replacement of the anterior cruciate ligament with carbon-fiber. J Bone Jt Surg Br, 1983, 65: 30-39.
15.	Wredmark T, Engstrom B. Five-year results of anterior cruciate ligament reconstruction with the Stryker Dacron high-strength ligament. Knee Surg Sports Traumatol Arthrosc, 1993, 1(2): 71-75.
16.	Frank C B, Jackson D W. The science of reconstruction of the anterior cruciate ligament. J Bone Joint Surg Am, 1997, 79(10): 1556-1576.
17.	Fukubayashi T, Ikeda K. Follow-up study of Gore-Tex artificial ligament—special emphasis on tunnel osteolysis. J Long Term Eff Med Implants, 2000, 10(4): 267-277.
18.	Miller M D, Peters C L, Allen B. Early aseptic loosening of a total knee arthroplasty due to Gore-Tex particle-induced osteolysis. J Arthroplasty, 2006, 21(5): 765-770.
19.	Hehl G, Kinzl L, Reichel R. Carbon-fiber implants for knee ligament reconstruction. 10-year results. Chirurg, 1997, 68(11): 1119-1125.
20.	Debnath U K, Fairclough J A, Williams R L. Long-term local effects of carbon fibre in the knee. Knee, 2004, 11(4): 259-264.
21.	史新立, 譚芳奕, 王召旭. 瘋牛病病原體研究及動物源性醫療器械產品安全性思考. 中國修復重建外科雜志, 2006, 20(11): 1138-1144.
22.	陶凱忠, 陳爾瑜, 丁光宏. 膠原纖維的結構和生物力學. 解剖科學進展雜志, 1998, 4(4): 289-293.
23.	張弛. 纖維連接蛋白表面修飾后聚酯纖維韌帶細胞相容性的變化. 中國組織工程研究與臨床康復, 2007, 11(31): 6149-6154.
24.	孫康, 王立德, 張羽飛, 等. 人工合成材料重建膝關節交叉韌帶的進展. 骨與關節損傷雜志, 2000, 15(3): 236-238.
25.	Barber F A, Burans J P, Deutsch A, et al. A prospective, randomized evaluation of acellular human dermal matrix augmentation for arthroscopic rotator cuff repair. Arthrroscope, 2012, 28(1): 8-15.
26.	金良. 生長因子包被的PLCL/纖維蛋白原靜電紡絲膜片與兔骨髓間充質干細胞的體外生物相容性研究. 合肥: 安徽醫科大學, 2012.
27.	尹玉霞, 鄭秀娥, 魯守濤, 等. 靜電紡絲及其在生物醫用材料中的應用. 中國醫療器械信息, 2019, 25(1): 46-47, 128.
28.	董鑫. 靜電紡絲納米纖維的制備及其在生物醫藥方面的應用. 中外醫療, 2008, 32: 154.
29.	王楓. 膝關節人工韌帶損傷與人工韌帶研究進展. 中國組織工程研究與臨床康復, 2010, 14(21): 3919-3922.
30.	侯立剛, 楊建義. 人工韌帶生物材料修復膝關節交叉韌帶損傷: 問題及前景. 中國組織工程研究, 2016, 20(8): 1196-1202.
31.	趙旭輝, 王芳. 人工韌帶材料表面修飾及在膝關節交叉韌帶重建中的應用. 中國組織工程研究與臨床康復, 2010, 14(42): 7915-7918.
32.	王昆, 朱蕾, 金文濤, 等. 生物型人工韌帶的體外檢測和體內組織相容性的實驗研究. 中國病理生理雜志, 2010, 26(12): 2394-2399.

1. Chen P, Zhu S, Wang Y, et al. The amelioration of cartilage degeneration by ADAMTS-5 inhibitor delivered in a hyaluronic acid hydrogel. Biomaterials, 2014, 35(9): 2827-2836.
2. Kim H S, Seon J K, Jo A R. Current trends in anterior cruciate ligament reconstruction. Knee Surg Relat Res, 2013, 25: 165-173.
3. Cohen M, Amaro J T, Ejnisman B, et al. Anterior cruciate ligament reconstruction after 10 to 15 years: association between meniscectomy and osteoarthrosis. Arthroscopy, 2007, 23: 629-634.
4. Buoncristiani A M, Tjoumakaris F P, Starman J S, et al. Anatomic double-bundle anterior cruciate ligament reconstruction. Arthroscopy, 2006, 22: 11-16.
5. Brunet P, Charrois O, Degeorges R, et a1. Reconstruction of acute posterior cruciate ligament tears usinga synthetic ligament. Rev Chir Orthop Reparatrice Appar Mot, 2005, 91(1): 34-43.
6. 余浩, 鄧晚秋, 桑鵬, 劉毅. 關節鏡下自體同側腓骨長肌腱重建膝關節前交叉韌帶. 中國修復重建外科雜志, 2020, 34(7): 843-847.
7. Schindler O S. Surgery for anterior cruciate ligament deficiency: a historical perspective. Knee Surg Sports Traumatol Arthrosc, 2012, 20: 5-7.
8. Seo Y K, Yoon H H, Song K Y, et al. Increase in cell migration and angiogenesis in a composite silk scaffold for tissue-engineered ligaments. J Orthop Res, 2009, 27: 495-503.
9. Schindler O S. Surgery for anterior cruciate ligament deficiency: a historical perspective. Knee Surg Sports Traumatol Arthrosc, 2012, 20: 45-47.
10. Murray A W, Macnicol M F. 10-16 years results of Leeds-Keio anterior cruciate ligament reconstruction. Knee, 2004, 11: 9-14.
11. Indelicato P A, Pascale M S, Huegel M O. Early experience with the GORE-TEX polytetrafluoroethylene anterior cruciate ligament prosthesis. Am J Sports Med, 1989, 17: 52-55.
12. Woods G A, Indelicato P A, Prevot T J. The Gore-Tex anterior cruciate ligament prosthesis. Two versus three year results. Am J Sports Med, 1991, 19: 48-55.
13. Maletius W, Gillquist J. Long-term results of anterior cruciate ligament reconstruction with a Dacron prosthesis. The frequency of osteoarthritis after seven to eleven years. Am J Sports Med, 1997, 25: 88-93.
14. Rushton N, Dandy D J, Naylor C P. The clinical, arthroscopic and histological findings after replacement of the anterior cruciate ligament with carbon-fiber. J Bone Jt Surg Br, 1983, 65: 30-39.
15. Wredmark T, Engstrom B. Five-year results of anterior cruciate ligament reconstruction with the Stryker Dacron high-strength ligament. Knee Surg Sports Traumatol Arthrosc, 1993, 1(2): 71-75.
16. Frank C B, Jackson D W. The science of reconstruction of the anterior cruciate ligament. J Bone Joint Surg Am, 1997, 79(10): 1556-1576.
17. Fukubayashi T, Ikeda K. Follow-up study of Gore-Tex artificial ligament—special emphasis on tunnel osteolysis. J Long Term Eff Med Implants, 2000, 10(4): 267-277.
18. Miller M D, Peters C L, Allen B. Early aseptic loosening of a total knee arthroplasty due to Gore-Tex particle-induced osteolysis. J Arthroplasty, 2006, 21(5): 765-770.
19. Hehl G, Kinzl L, Reichel R. Carbon-fiber implants for knee ligament reconstruction. 10-year results. Chirurg, 1997, 68(11): 1119-1125.
20. Debnath U K, Fairclough J A, Williams R L. Long-term local effects of carbon fibre in the knee. Knee, 2004, 11(4): 259-264.
21. 史新立, 譚芳奕, 王召旭. 瘋牛病病原體研究及動物源性醫療器械產品安全性思考. 中國修復重建外科雜志, 2006, 20(11): 1138-1144.
22. 陶凱忠, 陳爾瑜, 丁光宏. 膠原纖維的結構和生物力學. 解剖科學進展雜志, 1998, 4(4): 289-293.
23. 張弛. 纖維連接蛋白表面修飾后聚酯纖維韌帶細胞相容性的變化. 中國組織工程研究與臨床康復, 2007, 11(31): 6149-6154.
24. 孫康, 王立德, 張羽飛, 等. 人工合成材料重建膝關節交叉韌帶的進展. 骨與關節損傷雜志, 2000, 15(3): 236-238.
25. Barber F A, Burans J P, Deutsch A, et al. A prospective, randomized evaluation of acellular human dermal matrix augmentation for arthroscopic rotator cuff repair. Arthrroscope, 2012, 28(1): 8-15.
26. 金良. 生長因子包被的PLCL/纖維蛋白原靜電紡絲膜片與兔骨髓間充質干細胞的體外生物相容性研究. 合肥: 安徽醫科大學, 2012.
27. 尹玉霞, 鄭秀娥, 魯守濤, 等. 靜電紡絲及其在生物醫用材料中的應用. 中國醫療器械信息, 2019, 25(1): 46-47, 128.
28. 董鑫. 靜電紡絲納米纖維的制備及其在生物醫藥方面的應用. 中外醫療, 2008, 32: 154.
29. 王楓. 膝關節人工韌帶損傷與人工韌帶研究進展. 中國組織工程研究與臨床康復, 2010, 14(21): 3919-3922.
30. 侯立剛, 楊建義. 人工韌帶生物材料修復膝關節交叉韌帶損傷: 問題及前景. 中國組織工程研究, 2016, 20(8): 1196-1202.
31. 趙旭輝, 王芳. 人工韌帶材料表面修飾及在膝關節交叉韌帶重建中的應用. 中國組織工程研究與臨床康復, 2010, 14(42): 7915-7918.
32. 王昆, 朱蕾, 金文濤, 等. 生物型人工韌帶的體外檢測和體內組織相容性的實驗研究. 中國病理生理雜志, 2010, 26(12): 2394-2399.

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