Analysis of Treg Change and Experssion of Interferon-γ and Transforming Growth Factor-β1 in Patients with Premature Ovarian Failure_West China Medical Journal

Authors：

XIE Jiangyan ¹ , HE Wei ¹ , ZHAO Limei ¹ , CHEN Ming ² ,  LIANG Zhiqing. ¹

1. Department of Obstetrics and Gynecology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, P. R. China; 2. Medical Research Centre Southwest Hospital, Third Military Medical University, Chongqing, 400038, P. R. China;

Corresponding?author：

LIANG Zhiqing., Email: hello80318@163.com

Keywords：

卵巢功能早衰; T淋巴細胞，調節性; 干擾素Ⅱ型; 轉化生長因子-β 1

DOI：

10.7507/1002-0179.20130120

Video：

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目的　通過觀察卵巢早衰 POF 患者外周血CD4+CD25+調節性T細胞 Treg 及干擾素-γ（IFN-γ）、轉化生長因子-β(TGF-β)的變化，探討POF的免疫學發病機制。方法　收集2011年12月－2012年9月就診的POF患者17例，卵巢儲備功能減退 DOR 患者11例，以及生殖中心健康育齡女性16例，流式細胞儀定量檢測外周血Treg數量，Elisa方法檢測血清IFN-γ、TGF-β的水平，并以FSH/LH評價卵巢儲備功能，進行相關性分析。結果　與對照組相比，POF組和DOR組IFN-γ水平增高 P＜0.01 、TGF-β水平降低 P＜0.01 ，POF患者及DOR患者Treg比例降低 P＜0.01 ，IFN-γ的增高與卵巢儲備功能的下降呈顯著正相關 r=0.70，P＜0.01 。結論　 Treg 和IFN-γ、TGF-β水平與卵巢早衰密切相關，IFN-γ對評估卵巢儲備功能、預測卵巢早衰具有參考價值。

Citation： XIE Jiangyan,HE Wei,ZHAO Limei,CHEN Ming,LIANG Zhiqing.. Analysis of Treg Change and Experssion of Interferon-γ and Transforming Growth Factor-β1 in Patients with Premature Ovarian Failure. West China Medical Journal, 2013, 28(3): 377-379. doi: 10.7507/1002-0179.20130120 Copy

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4.	Voznesens’ka TIu, Serdiuk OM, Blashkiv TV, et al. Autoimmune mechanisms of ovarian pathology[J]. Fiziol Zh, 2011, 57(4): 90-95.
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8.	Nilsson EE, Doraiswamy V, Skinner MK. Transforming growth factor-beta isoform expression during bovine ovarian antral follicle development[J]. Mol Reprod Dev, 2003, 66(3): 237-246.
9.	Koenecke C, Lee CW, Thamm K, et al. IFN-γ production by allogeneic Foxp3+ regulatory T cells is essential for preventing experimental graft-versus-host disease[J]. J Immunol, 2012, 189(6): 2890-2896.
10.	Sawitzki B, Kingsley CI, Oliveira V, et al. IFN-γ production by alloantigen-reactive regulatory T cells is important for their regulatory function in vivo[J]. J Exp Med, 2005, 201(12): 1925- 1935.
11.	Wan YY, Flavell RA. TGF-β and regulatory T cell in immunity and Autoimmunity[J]. J Clin Immunol, 28(6): 647-659.
12.	Sirotkin AV. Cytokines: signalling molecules controlling ovarian functions[J]. Int J Biochem Cell Biol，2011, 43 (6) : 857-861.
13.	Sarapik A, Velthut A, Haller-Kikkatalo K, et al. Follicular proinflammatory cytokines and chemokines as markers of IVF success[J]. Clin Dev Immunol, 2012. (Epub 2011 Oct 5).
14.	Peng C. The TGF-beta superfamily and its roles in the human ovary and placenta[J]. Obstet Gynaecol Can, 2003, 25(10): 834-844.
15.	Daniel J.Trombly,Teresa K, et al. Roles for transforming growth factor beta superfamily proteins in early folliculogenesis[J]. Semin Reprod Med, 2009, 27(1): 14-23.
16.	Myers M, Pangas SA. Regulatory roles of transforming growth factor beta family members in folliculogenesis[J]. Wiley Interdiscip Rev Syst Biol Med, 2010, 2(1): 117- 125.
17.	Lourenco EV, Cava AL. Natural regulatory T cells in autoimmunity[J]. Autoimmunity, 2011, 44(1): 33-42.
18.	Qiao M, Thornton AM, Shevach EM. CD4+CD25+ regulatory T cells render naive CD4+CD25- T cells anergic and supressive[J]. Immunology, 2007, 120(4): 447- 455.
19.	Seckin B, Turkcapar F, Ozaksit G. Elevated day 3 FSH/LH ratio: a marker to predict IVF outcome in young and older women[J]. J Assist Reprod Genet, 2012, 29(3): 231- 236.

1. 　Shelling AN. Premature ovarian failure[J]. Reproduction, 2010，140(5): 633-641.
2. 　Maclaran K, Panay N. Premature ovarian failure[J]. J Fam Plann Reprod Health Care, 2011, 37(1): 35-42.
3. 　Dragojevi?-Diki? S, Marisavljevi? D, Mitrovi? A, et al. An immunological insight into premature ovarian failure (POF)[J] . Autoimmun Rev, 2010, 9(11): 771-774.
4. 　Voznesens’ka TIu, Serdiuk OM, Blashkiv TV, et al. Autoimmune mechanisms of ovarian pathology[J]. Fiziol Zh, 2011, 57(4): 90-95.
5. 　Yamada Y, Saton K. Folliculogenesis and regulating factors[J]. Nippon Runsho, 1997, 55(2): 2822.
6. 　Mermillod P, Dalbiès-Tran R, Uzbekova S, et al. Factors affecting oocyte quality: who is driving the follicle?[J]. Reprod Domest Anim, 2008, 43(Suppl 2): 393-400.
7. 　Russell P. The clinicopathological features of primary ovarian failure[J]. Verh Dtsch Ges Pathol, 1997, 81(6): 197-209.
8. 　Nilsson EE, Doraiswamy V, Skinner MK. Transforming growth factor-beta isoform expression during bovine ovarian antral follicle development[J]. Mol Reprod Dev, 2003, 66(3): 237-246.
9. 　Koenecke C, Lee CW, Thamm K, et al. IFN-γ production by allogeneic Foxp3+ regulatory T cells is essential for preventing experimental graft-versus-host disease[J]. J Immunol, 2012, 189(6): 2890-2896.
10. 　Sawitzki B, Kingsley CI, Oliveira V, et al. IFN-γ production by alloantigen-reactive regulatory T cells is important for their regulatory function in vivo[J]. J Exp Med, 2005, 201(12): 1925- 1935.
11. 　Wan YY, Flavell RA. TGF-β and regulatory T cell in immunity and Autoimmunity[J]. J Clin Immunol, 28(6): 647-659.
12. 　Sirotkin AV. Cytokines: signalling molecules controlling ovarian functions[J]. Int J Biochem Cell Biol，2011, 43 (6) : 857-861.
13. 　Sarapik A, Velthut A, Haller-Kikkatalo K, et al. Follicular proinflammatory cytokines and chemokines as markers of IVF success[J]. Clin Dev Immunol, 2012. (Epub 2011 Oct 5).
14. 　Peng C. The TGF-beta superfamily and its roles in the human ovary and placenta[J]. Obstet Gynaecol Can, 2003, 25(10): 834-844.
15. 　Daniel J.Trombly,Teresa K, et al. Roles for transforming growth factor beta superfamily proteins in early folliculogenesis[J]. Semin Reprod Med, 2009, 27(1): 14-23.
16. 　Myers M, Pangas SA. Regulatory roles of transforming growth factor beta family members in folliculogenesis[J]. Wiley Interdiscip Rev Syst Biol Med, 2010, 2(1): 117- 125.
17. 　Lourenco EV, Cava AL. Natural regulatory T cells in autoimmunity[J]. Autoimmunity, 2011, 44(1): 33-42.
18. 　Qiao M, Thornton AM, Shevach EM. CD4+CD25+ regulatory T cells render naive CD4+CD25- T cells anergic and supressive[J]. Immunology, 2007, 120(4): 447- 455.
19. 　Seckin B, Turkcapar F, Ozaksit G. Elevated day 3 FSH/LH ratio: a marker to predict IVF outcome in young and older women[J]. J Assist Reprod Genet, 2012, 29(3): 231- 236.

West China Medical Journal

Analysis of Treg Change and Experssion of Interferon-γ and Transforming Growth Factor-β1 in Patients with Premature Ovarian Failure

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