Evaluation of the Application of UF-1000i Automatic Urine Dreg Analyzer in Excluding Urinary Tract Infection_West China Medical Journal

Authors：

LUO Lan , HE Chao , LI Dongdong , WANG Liang ,  KANG Mei.

Clinical Microbiology Room, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding?author：

KANG Mei., Email: hxluolan@163.com

Keywords：

尿路感染; UF-1000i尿沉渣分析儀; 閾值

DOI：

10.7507/1002-0179.20130319

Video：

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目的　評價UF-1000i全自動尿沉渣分析儀中沉渣定量模塊中白細胞和細菌參數閾值在排除尿路感染的應用價值。方法　選取2 580份清潔中段尿液, 同時進行細菌培養菌落計數和UF-1000i尿沉渣白細胞和細菌定量分析，建立ROC曲線確定白細胞參數與細菌參數閾值。結果　以尿定量培養菌落計數G?菌≥105 cfu/mL，G+菌≥104 cfu/mL為陽性參考標準，當白細胞沉渣定量為100/μL時，UF-1000i尿沉渣分析儀檢測靈敏度為64%，特異度為75%，陰性預測值為96%；當UF-1000i細菌計量為901/μL時，檢測靈敏度為68.3%，特異度為92.8%，陰性預測值為97%。結論　UF-1000i檢測新鮮尿標本白細胞的測定值 lt;100/μL，細菌值 lt;901/μL時能夠作為臨床早期排除尿路感染的依據之一。

Citation： LUO Lan,HE Chao,LI Dongdong,WANG Liang,KANG Mei.. Evaluation of the Application of UF-1000i Automatic Urine Dreg Analyzer in Excluding Urinary Tract Infection. West China Medical Journal, 2013, 28(7): 1033-1035. doi: 10.7507/1002-0179.20130319 Copy

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3.	Kouri TT, Kähkönen U, Malminiemi K, et al. Evaluation of sysmex UF-100 urine flow cytometer vs chamber counting of supravitally stained specimens and conventional bacterial cultures[J]. Am J Clin Pathol, 1999, 112(1): 25-35.
4.	魏夢嵐, 陳偉, 高潔. UF-100尿沉渣分析儀在尿路感染早期診斷中的應用[J]. 現代醫藥衛生, 2008, 24(4): 483-484.
5.	寧永忠, 馬紀志, 張捷. 全自動尿沉渣分析儀UF-100中細菌計數參數閾值的研究[J]. 中國誤診學雜志, 2009, 9(36): 8824-8826.
6.	Koken T, Aktepe OC, Serteser M, et al. Determination of cut-off values for leucocytes and bacteria for urine flow cytometer (UF-100) in urinary tract infections[J]. Int Urol Nephrol, 2002, 34(2): 175-178.
7.	Evans R, Davidson MM, Sim LR, et al. Testing by sysmex UF-100 flow cytometer and with bacterial culture in a diagnostic laboratory: a comparison[J]. J Clin Pathol, 2006, 59(6): 661-662.
8.	Fenili D, Pirovano B. The automation of sediment urinalysis using a new urine flow cytometer (UF-100)[J]. Clin Chem Lab Med, 1998, 36(12): 909-917.
9.	馬筱玲, 李桂琴. 臨床微生物學尿培養操作規范[J]. 中華檢驗醫學雜志, 2005, 28(10): 115-117.
10.	Rosa RD, Grosso S, Bruschetta G, et al. Evaluation of the sysmex UF1000i flow cytometer for ruling out bacterial urinary tract infection[J]. Clin Chim Acta, 2010, 411(15-16): 1137-1142.
11.	魏宏蓮, 范世英, 王喜棟, 等. UF-100尿液分析儀在尿路感染篩檢中的應用[J]. 西部醫學, 2011, 23(2): 364-365.

1. 　黎國慶, 郭明好, 楊磊, 等. 膀胱穿刺尿同步細菌培養在女性尿路感染診斷中的價值[J]. 鄭州大學學報·醫學版, 2002, 37(2): 224-225.
2. 　Zaman Z, Roggeman S, Verhaegen J. Unsatisfactory performance of flow cytometer UF-100 and urine strips in predicting outcome of urine cultures[J]. J Clin Microbiol, 2001, 39(11): 4169-4171.
3. 　Kouri TT, Kähkönen U, Malminiemi K, et al. Evaluation of sysmex UF-100 urine flow cytometer vs chamber counting of supravitally stained specimens and conventional bacterial cultures[J]. Am J Clin Pathol, 1999, 112(1): 25-35.
4. 　魏夢嵐, 陳偉, 高潔. UF-100尿沉渣分析儀在尿路感染早期診斷中的應用[J]. 現代醫藥衛生, 2008, 24(4): 483-484.
5. 　寧永忠, 馬紀志, 張捷. 全自動尿沉渣分析儀UF-100中細菌計數參數閾值的研究[J]. 中國誤診學雜志, 2009, 9(36): 8824-8826.
6. 　Koken T, Aktepe OC, Serteser M, et al. Determination of cut-off values for leucocytes and bacteria for urine flow cytometer (UF-100) in urinary tract infections[J]. Int Urol Nephrol, 2002, 34(2): 175-178.
7. 　Evans R, Davidson MM, Sim LR, et al. Testing by sysmex UF-100 flow cytometer and with bacterial culture in a diagnostic laboratory: a comparison[J]. J Clin Pathol, 2006, 59(6): 661-662.
8. 　Fenili D, Pirovano B. The automation of sediment urinalysis using a new urine flow cytometer (UF-100)[J]. Clin Chem Lab Med, 1998, 36(12): 909-917.
9. 　馬筱玲, 李桂琴. 臨床微生物學尿培養操作規范[J]. 中華檢驗醫學雜志, 2005, 28(10): 115-117.
10. 　Rosa RD, Grosso S, Bruschetta G, et al. Evaluation of the sysmex UF1000i flow cytometer for ruling out bacterial urinary tract infection[J]. Clin Chim Acta, 2010, 411(15-16): 1137-1142.
11. 　魏宏蓮, 范世英, 王喜棟, 等. UF-100尿液分析儀在尿路感染篩檢中的應用[J]. 西部醫學, 2011, 23(2): 364-365.

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Evaluation of the Application of UF-1000i Automatic Urine Dreg Analyzer in Excluding Urinary Tract Infection

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