The Effect of Post-conditioning with Fospropofol Disodium on Hepatic Ischemia-reperfusion and Its Influence on the Expression of bcl-2/bax Protein in Rats_West China Medical Journal

Authors：

 HEYin ,  LUOChao-zhi

Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding?author：

LUOChao-zhi, Email: chaozhil@aliyun.com

Keywords：

Fospropofol disodium; Hepatic ischemia-reperfusion; Post-conditioning; Protective effects; bcl-2/bax protein; Rats

DOI：

10.7507/1002-0179.20140382

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the effect of post-conditioning with fospropofol disodium on hepatic ischemiareperfusion (I/R) and its possible mechanism in rats. Methods Forty-eight Sprague-Dawley rats were randomly divided into four groups, including sham group (S), control group (C), propofol group (P) and fospropofol disodium group (F). According to the different periods after reperfusion, each group was further divided into 2-hour and 4-hour reperfusion subgroups respectively (n=6 in each subgroup), named S_2h, C_2h, P_2h, and F_2h subgroups and S_4h, C_4h, P_4h, and F_4h subgroups. The livers of rats were reperfused after hepatic ischemia for one hour. In the beginning of reperfusion, normal saline was infused intravenously in group S and group C continuously, propofol was infused intravenously in group P continuously, fospropofol disodium was infused continuously in group F. The blood was sampled at the end of ischemia and reperfusion for assay of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The bcl-2 and bax protein contents in liver tissue were detected by immunohistochemical analysis, and liver samples were stained with hematoxylin-eosine for histological observation and damage degree evaluation by counting the proportion of necrosis cells. Results The activity of ALT and AST, the rate of necrosis cells and the amount of bcl-2 and bax protein after reperfusion in group C, group P and group F were higher than those in group S at matched reperfusion time points (P<0.05). The activity of ALT and AST, the proportion of necrosis cells and bax protein contents decreased in group P and group F, compared with group C at the same reperfusion time points, while the contents of bcl-2 protein were significantly increased (P<0.05). Conclusion Fospropofol disodium can alleviate hepatic injury induced by ischemia-reperfusion in rats, in which the bcl-2 and bax protein may play important roles.

Citation： HEYin, LUOChao-zhi. The Effect of Post-conditioning with Fospropofol Disodium on Hepatic Ischemia-reperfusion and Its Influence on the Expression of bcl-2/bax Protein in Rats. West China Medical Journal, 2014, 29(7): 1242-1247. doi: 10.7507/1002-0179.20140382 Copy

1.	肖峰, 周曉清, 李元濤, 等. 異丙酚對大鼠缺血再灌注海馬區Bax和Bcl-2表達的影響[J]. 華中科技大學學報·醫學版, 2010, 39(1):113-115, 119.
2.	李錦, 王莉莉, 李樹人. 丙泊酚對大鼠腎缺血再灌注損傷細胞凋亡信號通路的影響[J]. 中國組織工程研究與臨床康復, 2009, 13(31):6013-6017.
3.	馮春生, 麻海春, 岳云, 等. 異丙酚對大鼠局灶性腦缺血再灌注時NF-κB活化和Bcl-2、Caspase-3基因表達的影響[J]. 中華麻醉學雜志, 2006, 26(5):456-459.
4.	劉宇, 羅朝志. 磷丙泊酚鈉對大鼠在體肝臟缺血再灌注損傷的保護作用[J]. 四川大學學報·醫學版, 2011, 42(2):231-233.
5.	張琰, 冷玉芳, 方七五. 異丙酚后處理聯合缺血后處理對大鼠肝臟缺血再灌注損傷的影響[J]. 中華麻醉學雜志, 2010, 30(1):86-89.
6.	元文勇, 余偉平, 葉啟發, 等. 大鼠肝臟缺血再灌注損傷程度判定指標的選擇[J]. 中國普通外科雜志, 2008, 17(7):650-653.
7.	王澤華, 殷振奎, 黃海波, 等. 丙泊酚對急性腎缺血再灌注過程脂質過氧化損傷的保護作用[J]. 臨床麻醉學雜志, 2001, 17(11):619-621.
8.	Atlas G. Fospropofol:is there an infusion regimen for propofol equivalence?[J]. J Anaesthesiol Clin Pharmacol, 2011, 27(3):303-306.
9.	Chu ZL, Mckinsey T, Liu L, et al. Suppression of tumor necrosis factor-induced cell death by inhibitor of apoptosis c-IAP2 is under NF-κB control[J]. Proc Nati Acad Sci USA, 1997, 94(19):10057-10062.
10.	Deng WL, Wang D, Gosmanova E, et al. LPA protects intestinal epithelial cells from apoptosis by inhibiting the mitochondrial pathway[J]. Am J Physiol Gastrointest Liver Physiol, 2003, 284(5):G821-G829.
11.	Guiral JS, Nleci TJ, Farhood A, et al. Mechanism of Celt death during warm hepatic ischemia-reperfmion in rats:apoptosis or necrosis?[J]. Hepatology, 2001, 33(2):397-405.
12.	Yamabe K, Shimizu S, Kamiike W, et al. Prevention of hypoxic liver cell necrosis by in vivo human bcl-2 gene transfection[J]. Biochem Biophys Res Commun, 1998, 243(1):217-223.
13.	Kane DJ, Ord T, Anton R, et al. Expression of bcl-2 inhibits necrotic neural cell death[J]. J Neurosci Res, 1995, 40(2):269-275.
14.	張瑋瑋, 郭永清. 丙泊酚對大鼠肝臟缺血再灌注損傷Bcl-2和P53表達的影響[J]. 山西醫藥雜志, 2008, 37(19):892-894.
15.	Vollmar B, Glasz J, Leiderer R, et al. Hepatic microcirculatory perfusion failure is a determinant for liver dysfunction in warm ischemia-reperfusion[J]. Ampatol, 1994, 145(6):1421-1423.
16.	Jaeschke H. Molecular mechanisms of hepatic ischemia-reperfusion injury and preconditioning[J]. Am J Physiol Gastrointest Liver Physiol, 2003, 284(1):G15-G26.
17.	袁苑, 曹定睿, 殷淵. 丙泊酚對大鼠腸缺血再灌注時肝損傷及Bcl-2、Bax蛋白表達的影響[J]. 山西醫科大學學報, 2008, 39(9):791-793.
18.	Campion ME, Gan TJ. Fospropofol disodium for sedation[J]. Drugs Today, 2009, 45(8):567-576.

1. 肖峰, 周曉清, 李元濤, 等. 異丙酚對大鼠缺血再灌注海馬區Bax和Bcl-2表達的影響[J]. 華中科技大學學報·醫學版, 2010, 39(1):113-115, 119.
2. 李錦, 王莉莉, 李樹人. 丙泊酚對大鼠腎缺血再灌注損傷細胞凋亡信號通路的影響[J]. 中國組織工程研究與臨床康復, 2009, 13(31):6013-6017.
3. 馮春生, 麻海春, 岳云, 等. 異丙酚對大鼠局灶性腦缺血再灌注時NF-κB活化和Bcl-2、Caspase-3基因表達的影響[J]. 中華麻醉學雜志, 2006, 26(5):456-459.
4. 劉宇, 羅朝志. 磷丙泊酚鈉對大鼠在體肝臟缺血再灌注損傷的保護作用[J]. 四川大學學報·醫學版, 2011, 42(2):231-233.
5. 張琰, 冷玉芳, 方七五. 異丙酚后處理聯合缺血后處理對大鼠肝臟缺血再灌注損傷的影響[J]. 中華麻醉學雜志, 2010, 30(1):86-89.
6. 元文勇, 余偉平, 葉啟發, 等. 大鼠肝臟缺血再灌注損傷程度判定指標的選擇[J]. 中國普通外科雜志, 2008, 17(7):650-653.
7. 王澤華, 殷振奎, 黃海波, 等. 丙泊酚對急性腎缺血再灌注過程脂質過氧化損傷的保護作用[J]. 臨床麻醉學雜志, 2001, 17(11):619-621.
8. Atlas G. Fospropofol:is there an infusion regimen for propofol equivalence?[J]. J Anaesthesiol Clin Pharmacol, 2011, 27(3):303-306.
9. Chu ZL, Mckinsey T, Liu L, et al. Suppression of tumor necrosis factor-induced cell death by inhibitor of apoptosis c-IAP2 is under NF-κB control[J]. Proc Nati Acad Sci USA, 1997, 94(19):10057-10062.
10. Deng WL, Wang D, Gosmanova E, et al. LPA protects intestinal epithelial cells from apoptosis by inhibiting the mitochondrial pathway[J]. Am J Physiol Gastrointest Liver Physiol, 2003, 284(5):G821-G829.
11. Guiral JS, Nleci TJ, Farhood A, et al. Mechanism of Celt death during warm hepatic ischemia-reperfmion in rats:apoptosis or necrosis?[J]. Hepatology, 2001, 33(2):397-405.
12. Yamabe K, Shimizu S, Kamiike W, et al. Prevention of hypoxic liver cell necrosis by in vivo human bcl-2 gene transfection[J]. Biochem Biophys Res Commun, 1998, 243(1):217-223.
13. Kane DJ, Ord T, Anton R, et al. Expression of bcl-2 inhibits necrotic neural cell death[J]. J Neurosci Res, 1995, 40(2):269-275.
14. 張瑋瑋, 郭永清. 丙泊酚對大鼠肝臟缺血再灌注損傷Bcl-2和P53表達的影響[J]. 山西醫藥雜志, 2008, 37(19):892-894.
15. Vollmar B, Glasz J, Leiderer R, et al. Hepatic microcirculatory perfusion failure is a determinant for liver dysfunction in warm ischemia-reperfusion[J]. Ampatol, 1994, 145(6):1421-1423.
16. Jaeschke H. Molecular mechanisms of hepatic ischemia-reperfusion injury and preconditioning[J]. Am J Physiol Gastrointest Liver Physiol, 2003, 284(1):G15-G26.
17. 袁苑, 曹定睿, 殷淵. 丙泊酚對大鼠腸缺血再灌注時肝損傷及Bcl-2、Bax蛋白表達的影響[J]. 山西醫科大學學報, 2008, 39(9):791-793.
18. Campion ME, Gan TJ. Fospropofol disodium for sedation[J]. Drugs Today, 2009, 45(8):567-576.

West China Medical Journal

The Effect of Post-conditioning with Fospropofol Disodium on Hepatic Ischemia-reperfusion and Its Influence on the Expression of bcl-2/bax Protein in Rats

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content