Comparative Research between Fluorogenic Quantitative Polymerase Chain Reaction and Enzyme-linked Immunosorbent Assay Applied in Diagnosing Enterovirus 71 Infection_West China Medical Journal

Authors：

 YANGXing-lin ¹ , XIONGJin-feng ¹ , LILi ² , WANGYi-guang ¹

1. Department of Laboratory;Guizhou No. 5 People's Hospital of Guiyang, Guiyang, Guizhou 55000, P. R. China;
2. Department of Infection;Guizhou No. 5 People's Hospital of Guiyang, Guiyang, Guizhou 55000, P. R. China;

Corresponding?author：

Keywords：

Hand-foot-mouth disease; Enterovirus 71; IgM antibody; Enterovirus 71 nucleic acid

DOI：

10.7507/1002-0179.20140399

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Objective To compare effect of enterovirus (EV) 71 nucleic acid detection and EV71-IgM antibody detection on clinically diagnosis of hand-foot-mouth disease in children. Methods Rectal swabs collected from 1379 children who were clinically diagnosed from April 20, 2011 to September 10, 2011 as suspected patients with the handfoot- mouth disease were detected by fluorogenic quantitative polymerase chain reaction to conduct EV71 nucleic acid detection. Meantime, enzyme-linked immunosorbent assay was used to conduct EV71-IgM antibody detection in serum samples collected from those children. Results In these 1379 cases, 79 had positive EV71 nucleic acids with a positive rate of 5.73%; while 82 cases had positive EV71-IgM antibodies with a positive rate of 5.95%. There were 32 cases with positive EV71 nucleic acid and positive EV71-IgM antibody. The rate of consistent results of two detection methods was 95.2%. The positive rates of two methods had no negligible differences (χ²=0.093, P=0.761). Conclusion Combination of EV71 nucleic acid detection and EV71-IgM antibody detection, can improve the efficiency in diagnosing hand-foot-mouth disease in children and facilitate the protection and diagnosis of the disease.

Citation： YANGXing-lin, XIONGJin-feng, LILi, WANGYi-guang. Comparative Research between Fluorogenic Quantitative Polymerase Chain Reaction and Enzyme-linked Immunosorbent Assay Applied in Diagnosing Enterovirus 71 Infection. West China Medical Journal, 2014, 29(7): 1309-1312. doi: 10.7507/1002-0179.20140399 Copy

1.	Hamaguchi T, Fujisawa H, Sakai K, et al. Acute encephalitis caused by intrafamilial transmission of enterovirus 71 in adult[J]. Emerg Infect Dis, 2008, 14(5):828-830.
2.	Schmidt NJ, Lennette EH, Ho HH. An apparently new enterovirus isolated from patients with disease of the central nervous system[J]. J Infect Dis, 1974, 129(3):304-309.
3.	何家鑫, 王碧錦, 奚月蘭, 等. 柯薩奇A16引起"手足口"病流行的特點簡報[J]. 中國人獸共患病雜志, 1985, 1(2):36.
4.	毛國順, 羅玲, 劉曉琳, 等. 手足口病輕癥與重癥患者臨床特征比較[J]. 中華傳染病雜志, 2008, 26(7):387-390.
5.	鄧莉, 張艷玲. 手足口病的流行與防治[J]. 北京醫學, 2008, 30(6):354-355.
6.	趙華, 趙春芳, 陳應瓊, 等. 重慶市2009年手足口病病原學分析[J]. 現代預防醫學, 2011, 38(12):2384-2385.
7.	李亮, 朱鳳才, 史智揚, 等. 江蘇省手足口病病原學流行特征分析[J]. 中華預防醫學雜志, 2010, 44(7):676-677.
8.	朱冰, 鐘家禹, 鄺璐, 等. 2010年廣州地區兒童腸道病毒71型感染的分子流行病學研究[J]. 中華醫院感染學雜志, 2011, 21(12):2401-2403.
9.	杜陽光, 楊晉川, 晏嘉璐, 等. 血清中EV71型IgM抗體檢測對手足口病診斷的意義[J]. 中華全科醫學, 2011, 9(10):1574-1575.
10.	彭蘭芬, 張少豐, 付文金, 等. 腸病毒EV71-IgM抗體與核酸檢測在手足口病診斷中的價值[J]. 中國熱帶醫學, 2011, 11(3):274-275.
11.	Tan EL, Chow VT, Quak SH, et al. Development of multiplex real time hybridization probe reverse transcriptase polymerase chain reaction for specific detection and differentiation of Enterovirus 71 and Coxsackievirus A16[J]. Diagn Microbiol Infect Dis, 2008, 61(3):294-301.
12.	楊秀惠, 嚴延生. 手足口病的病原學研究進展[J]. 傳染病信息, 2008, 21(3):129-131.
13.	林裕龍, 溫坤, 王壓娣, 等. 捕獲ELISA法測定抗EV71-IgM抗體用于EV71感染早期診斷[J]. 臨床檢驗雜志, 2012, 30(1):27-28.
14.	謝靖婧, 楊桂林, 劉映霞, 等. 腸道病毒71型手足口病ELISA診斷試劑盒研制與臨床應用[J]. 中華檢驗醫學雜志, 2009, 32(11):1262-1265.

1. Hamaguchi T, Fujisawa H, Sakai K, et al. Acute encephalitis caused by intrafamilial transmission of enterovirus 71 in adult[J]. Emerg Infect Dis, 2008, 14(5):828-830.
2. Schmidt NJ, Lennette EH, Ho HH. An apparently new enterovirus isolated from patients with disease of the central nervous system[J]. J Infect Dis, 1974, 129(3):304-309.
3. 何家鑫, 王碧錦, 奚月蘭, 等. 柯薩奇A16引起"手足口"病流行的特點簡報[J]. 中國人獸共患病雜志, 1985, 1(2):36.
4. 毛國順, 羅玲, 劉曉琳, 等. 手足口病輕癥與重癥患者臨床特征比較[J]. 中華傳染病雜志, 2008, 26(7):387-390.
5. 鄧莉, 張艷玲. 手足口病的流行與防治[J]. 北京醫學, 2008, 30(6):354-355.
6. 趙華, 趙春芳, 陳應瓊, 等. 重慶市2009年手足口病病原學分析[J]. 現代預防醫學, 2011, 38(12):2384-2385.
7. 李亮, 朱鳳才, 史智揚, 等. 江蘇省手足口病病原學流行特征分析[J]. 中華預防醫學雜志, 2010, 44(7):676-677.
8. 朱冰, 鐘家禹, 鄺璐, 等. 2010年廣州地區兒童腸道病毒71型感染的分子流行病學研究[J]. 中華醫院感染學雜志, 2011, 21(12):2401-2403.
9. 杜陽光, 楊晉川, 晏嘉璐, 等. 血清中EV71型IgM抗體檢測對手足口病診斷的意義[J]. 中華全科醫學, 2011, 9(10):1574-1575.
10. 彭蘭芬, 張少豐, 付文金, 等. 腸病毒EV71-IgM抗體與核酸檢測在手足口病診斷中的價值[J]. 中國熱帶醫學, 2011, 11(3):274-275.
11. Tan EL, Chow VT, Quak SH, et al. Development of multiplex real time hybridization probe reverse transcriptase polymerase chain reaction for specific detection and differentiation of Enterovirus 71 and Coxsackievirus A16[J]. Diagn Microbiol Infect Dis, 2008, 61(3):294-301.
12. 楊秀惠, 嚴延生. 手足口病的病原學研究進展[J]. 傳染病信息, 2008, 21(3):129-131.
13. 林裕龍, 溫坤, 王壓娣, 等. 捕獲ELISA法測定抗EV71-IgM抗體用于EV71感染早期診斷[J]. 臨床檢驗雜志, 2012, 30(1):27-28.
14. 謝靖婧, 楊桂林, 劉映霞, 等. 腸道病毒71型手足口病ELISA診斷試劑盒研制與臨床應用[J]. 中華檢驗醫學雜志, 2009, 32(11):1262-1265.

West China Medical Journal

Comparative Research between Fluorogenic Quantitative Polymerase Chain Reaction and Enzyme-linked Immunosorbent Assay Applied in Diagnosing Enterovirus 71 Infection

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