Determination of the Concentration of Isonicotinyl Hydrazide in the Hydrothorax of Tuberculosis Patients_West China Medical Journal

Authors：

 ZOUPin-jiao ,  WUJuan , JIANGYun-ping , JINWei-hua , JIANGYan , YUBo-tao , FANKai-hua

Department of Pharmacy, General Hospital of Chengdu Military Region, Chengdu, Sichuan 610083, P. R. China;

Corresponding?author：

WUJuan, Email: morriswujuan@126.com

Keywords：

Isonicotinyl hydrazide; High performance liquid chromatography; Tuberculosis patients; Hydrothorax

DOI：

10.7507/1002-0179.20140455

Video：

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Objective To determinate the concentration of isonicotinyl hydrazide (INH) in the hydrothorax and thereby increase the drug concentration of the hydrothorax to enhance the anti-tuberculosis efficacy through experiments. Methods Between February and June 2009, we used high performance liquid chromatography (HPLC) to determinate the concentration of INH in the hydrothorax of tuberculosis (TB) patients. The separation of sample was performed on Agilent ZORBAX SB-C18 (5 μm, 4.6 mm×2.5 mm) column with a fixed sample injection volume of 20 μL. The mobile phase was methanol-water (20︰80) at a flow rate of 1 mL/min. The ultraviolet detective wavelength was set at 254 nm; The column temperature was room temperature. Results In the range of 0.25-10.00 μg/mL (r=0.998 9), moxi-floxacin showed a good linear relation in HPLC. The recoveries of moxi-floxacin at three concentrations (0.5, 5.0, and 10.0 μg/mL) were 97.95%, 100.64%, and 102.84%, respectively, with intra-day relative standard deviation (RSD) at 3.49%, 1.45%, and 2.03%, respectively and intra-day RSD at 3.85%, 5.68%, 4.15%, respectively. Conclusion The measuring method adopted in the experiment can accurately determinate the concentration of INH in the hydrothorax with high sensitivity and exceptional reproducibility.

Citation： ZOUPin-jiao, WUJuan, JIANGYun-ping, JINWei-hua, JIANGYan, YUBo-tao, FANKai-hua. Determination of the Concentration of Isonicotinyl Hydrazide in the Hydrothorax of Tuberculosis Patients. West China Medical Journal, 2014, 29(8): 1483-1486. doi: 10.7507/1002-0179.20140455 Copy

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13.	劉會民, 張潔, 張永進. 我院異煙肼血藥濃度監測結果分析[J]. 天津藥學, 2011, 23(6):27-28.
14.	Liu P, Fu ZZ, Jiang JM, et al. Determination of isoniazid concentration in rabbit vertebrae by isotope tracing technique in conjunction with HPLC[J]. Biomed Chromatogr, 2013, 27(9):1150-1156.
15.	戈朝暉, 王自立, 魏敏吉, 等. RP-HPLC法測定脊柱結核病椎組織中異煙肼的濃度[J]. 脊柱外科雜志, 2008, 6(3):165-168, 190.
16.	徐康平, 李福雙, 譚健兵, 等. 川東獐牙菜藥材的HPLC指紋圖譜初步研究[J]. 中南藥學, 2004, 2(1):10-13.
17.	紀蘭菊, 保怡, 陳桂琛, 等. 15種獐牙菜屬植物中主要藥用成分的高效液相色譜測定[J]. 西北植物學報, 2004, 2(7):1298-1302.
18.	曾德貴, 劉玉琴. 異煙肼人體血藥濃度測定及藥代動力學研究[J]. 中國藥業, 2011, 20(3):6-7.

1. 王超, 朱中元. 抗結核病藥物的研究進展[J]. 中國熱帶醫學, 2008, 8(4):673-675.
2. 何國鈞. 抗結核藥物的研究進展[J]. 中國防癆雜志, 1996, 23(3):136-139.
3. Mitchison DA. Role of individual drugs in the chemotherapy of tuberculosis[J]. Int J Tuberc Lung Dis, 2000, 4(9):796-806.
4. 丁輝. HPLC法測定異煙肼片的含量[J]. 中華醫學實踐雜志, 2005, 4(8):813-814.
5. Liapakis IE, Kottakis I, Tzatzarakis MN, et al. Penetration of newer quinolones in the empyema fluid[J]. Eur Respir J, 2004, 24(3):466-740.
6. Jutte PC, Rutgers SR, Van Altena R. Penetration of isoniazid, rifampicin and pyrazinamide in tuberculous pleural effusion and psoas abscess[J]. Int J Tuberc Lung Dis, 2004, 8(11):1368-1372.
7. 楊德柱, 李文清, 王開平, 等. 高效液相色譜法測定異煙肼固體制劑血藥濃度[J]. 黑龍江醫藥, 1996, 9(2):68-69.
8. 鄧立東, 蔣學華, 陳鈞, 等. 異福膠囊的人體相對生物利用度研究[J]. 中國現代應用藥學, 2004, 21(2):132-136.
9. 黎昌權. 高效液相色譜法測定異煙肼注射液的含量[J]. 中國藥業, 2008, 17(8):32-33.
10. 趙偉杰, 段連山, 李惠文. HPLC法測定人血漿異煙肼濃度[J]. 結核病與胸部腫瘤, 1997, 1(2):43-45.
11. 成詩明. 全球結核病流行特征與控制進展[J]. 中國健康教育, 2005, 21(7):499-502.
12. 劉劍君, 幺鴻雁. 我國結核病的流行現狀和防治對策[J]. 預防醫學文獻信息, 2004, 10(3):381-384.
13. 劉會民, 張潔, 張永進. 我院異煙肼血藥濃度監測結果分析[J]. 天津藥學, 2011, 23(6):27-28.
14. Liu P, Fu ZZ, Jiang JM, et al. Determination of isoniazid concentration in rabbit vertebrae by isotope tracing technique in conjunction with HPLC[J]. Biomed Chromatogr, 2013, 27(9):1150-1156.
15. 戈朝暉, 王自立, 魏敏吉, 等. RP-HPLC法測定脊柱結核病椎組織中異煙肼的濃度[J]. 脊柱外科雜志, 2008, 6(3):165-168, 190.
16. 徐康平, 李福雙, 譚健兵, 等. 川東獐牙菜藥材的HPLC指紋圖譜初步研究[J]. 中南藥學, 2004, 2(1):10-13.
17. 紀蘭菊, 保怡, 陳桂琛, 等. 15種獐牙菜屬植物中主要藥用成分的高效液相色譜測定[J]. 西北植物學報, 2004, 2(7):1298-1302.
18. 曾德貴, 劉玉琴. 異煙肼人體血藥濃度測定及藥代動力學研究[J]. 中國藥業, 2011, 20(3):6-7.

West China Medical Journal

Determination of the Concentration of Isonicotinyl Hydrazide in the Hydrothorax of Tuberculosis Patients

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