Clinical Value of DNA Ploidy Analysis in the Diagnosis of Benignancy and Malignancy with Bronchial Brushing or Bronchoalveolar Lavage Fluid_West China Medical Journal

Authors：

 MAOZhi-yuan ¹ , XIONGMei ² , RENLi ¹ , LIWei ³ , LIDe-chang ¹ , YUEYing ¹ , ZHENGJi-chun ¹

1. Department of Pathology, Air Force General Hospital of PLA, Beijing, 100142, P. R. China;
2. Department of Blood Transfusion, Air Force General Hospital of PLA, Beijing, 100142, P. R. China;
3. Canada/China Cancer Center, Tongji Medical College, Wuhan, Hubei 430022, P. R. China;

Corresponding?author：

Keywords：

Bronchial brushing; Bronchoalveolar lavage fluid; DNA ploidy analysis; Cytological investigation

DOI：

10.7507/1002-0179.20140673

Video：

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Objective To investigate the clinical value of DNA ploidy analysis system in the diagnosis of benignancy or malignancy with bronchial brushing or bronchoalveolar lavage fluid. Methods We studied 96 bronchial brush tablets or bronchoalveolar lavage fluid specimens confirmed pathologically between June 2012 and June 2013, in which there were 49 cases of benignancy and 47 malignancies. Bronchial brush pieces were acquired by clinicians when they performed bronchoscopy for the patients. Each bronchoalveolar lavage fluid specimen was made into two slides, of which one was stained by HE method for cytology analysis, and the other was stained with Feulgen method for DNA ploidy analysis through automatic imaging cytometer. Results The specificity, sensitivity and accuracy of the routine cytological investigation were respectively 85.7%, 78.7% and 77.1%, while the three indexes for DNA ploidy analysis were 100.0%, 91.5% and 95.8%, respectively. Conclusion DNA ploidy analysis can improve the bronchial brushing or bronchoalveolar lavage fluid positive rate, and compared with cytological investigation, it is more specific and more sensitive with a high clinical value.

Citation： MAOZhi-yuan, XIONGMei, RENLi, LIWei, LIDe-chang, YUEYing, ZHENGJi-chun. Clinical Value of DNA Ploidy Analysis in the Diagnosis of Benignancy and Malignancy with Bronchial Brushing or Bronchoalveolar Lavage Fluid. West China Medical Journal, 2014, 29(12): 2226-2229. doi: 10.7507/1002-0179.20140673 Copy

1.	榮福, 蕭淑華, 劉靜, 等. 常規經支氣管鏡針吸活檢與超聲引導下經支氣管鏡針吸活檢對縱隔病變診斷的比較[J]. 中華結核和呼吸雜志, 2011, 34(2):120-122.
2.	王其生. 臨床腫瘤細胞學檢查[M]. 合肥:安徽科技出版社, 2005:51-71.
3.	操鳳, 張焜和. 漿膜腔積液的分子生物學診斷[J]. 國外醫學·腫瘤學分冊, 2005, 32(3):199-201.
4.	Auer G, Caspersson T, Wallgern A. DNA content and survical in mammary carcinoma[J]. Anat Quant Cytol, 1980, 2(3):161-165.
5.	張春來. 支氣管鏡下肺活檢并刷檢和經皮肺穿刺對中央型肺癌的診斷價值[J]. 臨床合理用藥雜志, 2011, 4(17):73-75.
6.	陶敏, 李建勇, 吳潔, 等. 支氣管沖洗和肺泡灌洗液細胞DNA含量分析對肺癌的診斷價值[J]. 中華結核和呼吸雜志, 1996, 19(4):31-34.
7.	翟志敏, 戴海明, 張明黎, 等. DNA含量測定對惡性腹水的診斷價值[J]. 癌癥, 2002, 21(1):105-106.
8.	Osterheld MC, Liette C, Anca M. Image cytometry:an aid for cytological diagnosis of pleural effusions[J]. Diagn Cytopathol,2005, 32(3):173-176.
9.	孫小蓉, 汪鍵, Boecking A. DNA倍體分析系統用于宮頸癌及上皮內瘤變的診斷及預測[J]. 中華病理學雜志, 2005, 34(7):435-437.
10.	Liu J, Katz R, Shin HJ, et al. Use of mailed urine specimens in diagnosing urothelial carcinoma by cytology and DNA image analysis[J]. Acta Cytol, 2005, 49(2):157-162.
11.	王麗萍, 馬俊, 梁若玲, 等. 食管黏膜脫落細胞DNA倍體檢測在食管癌診斷中的價值[J]. 廣東醫學, 2013, 36(16):2471-2474.
12.	何敏, 鄺軍, 朱建勇, 等. 痰細胞學聯合支氣管鏡DNA倍體檢測對肺癌的診斷意義[J]. 臨床肺科雜志, 2014, 19(4):710-712.
13.	郭超楠, 任力, 李煒, 等. DNA倍體分析系統在脫落細胞學及術中病理診斷中的應用[J]. 中國婦幼保健, 2013, 28(5):848-851.
14.	李德昌, 岳穎, 鄭吉春, 等. 組織印片DNA倍體分析在胃癌診斷和預后評估中的應用[J]. 空軍醫學雜志, 2014, 30(1):48-50.
15.	熊貴忠, 牛曉泉, 孫小蓉. 用DNA倍體分析評估口腔黏膜疾病嚴重程度[J]. 臨床口腔醫學雜志, 2013, 29(6):355-357.
16.	戴一菲, 張平安. 細針穿刺細胞學檢查結合DNA倍體分析在乳腺腫塊診斷中的應用[J]. 中華臨床醫師雜志, 2013, 12(7):5587-5589.
17.	蔡元菊, 黃文波, 馬占忠. 卵巢腫瘤細胞DNA倍體和S期細胞比率的檢測及臨床意義[J]. 中華臨床醫師雜志, 2013, 7(7):3196-3197.
18.	刑應如, 胡萬發, 楊路寶. DNA倍體聯合腫瘤標志物檢測在胸腔積液診斷中的應用[J]. 中國腫瘤外科雜志, 2013, 5(3):151-155.
19.	宋曉兵. HPV感染聯合細胞DNA檢測在宮頸癌篩查中的臨床應用價值[J]. 中國實用醫藥, 2014, 9(7):90-91.

1. 榮福, 蕭淑華, 劉靜, 等. 常規經支氣管鏡針吸活檢與超聲引導下經支氣管鏡針吸活檢對縱隔病變診斷的比較[J]. 中華結核和呼吸雜志, 2011, 34(2):120-122.
2. 王其生. 臨床腫瘤細胞學檢查[M]. 合肥:安徽科技出版社, 2005:51-71.
3. 操鳳, 張焜和. 漿膜腔積液的分子生物學診斷[J]. 國外醫學·腫瘤學分冊, 2005, 32(3):199-201.
4. Auer G, Caspersson T, Wallgern A. DNA content and survical in mammary carcinoma[J]. Anat Quant Cytol, 1980, 2(3):161-165.
5. 張春來. 支氣管鏡下肺活檢并刷檢和經皮肺穿刺對中央型肺癌的診斷價值[J]. 臨床合理用藥雜志, 2011, 4(17):73-75.
6. 陶敏, 李建勇, 吳潔, 等. 支氣管沖洗和肺泡灌洗液細胞DNA含量分析對肺癌的診斷價值[J]. 中華結核和呼吸雜志, 1996, 19(4):31-34.
7. 翟志敏, 戴海明, 張明黎, 等. DNA含量測定對惡性腹水的診斷價值[J]. 癌癥, 2002, 21(1):105-106.
8. Osterheld MC, Liette C, Anca M. Image cytometry:an aid for cytological diagnosis of pleural effusions[J]. Diagn Cytopathol,2005, 32(3):173-176.
9. 孫小蓉, 汪鍵, Boecking A. DNA倍體分析系統用于宮頸癌及上皮內瘤變的診斷及預測[J]. 中華病理學雜志, 2005, 34(7):435-437.
10. Liu J, Katz R, Shin HJ, et al. Use of mailed urine specimens in diagnosing urothelial carcinoma by cytology and DNA image analysis[J]. Acta Cytol, 2005, 49(2):157-162.
11. 王麗萍, 馬俊, 梁若玲, 等. 食管黏膜脫落細胞DNA倍體檢測在食管癌診斷中的價值[J]. 廣東醫學, 2013, 36(16):2471-2474.
12. 何敏, 鄺軍, 朱建勇, 等. 痰細胞學聯合支氣管鏡DNA倍體檢測對肺癌的診斷意義[J]. 臨床肺科雜志, 2014, 19(4):710-712.
13. 郭超楠, 任力, 李煒, 等. DNA倍體分析系統在脫落細胞學及術中病理診斷中的應用[J]. 中國婦幼保健, 2013, 28(5):848-851.
14. 李德昌, 岳穎, 鄭吉春, 等. 組織印片DNA倍體分析在胃癌診斷和預后評估中的應用[J]. 空軍醫學雜志, 2014, 30(1):48-50.
15. 熊貴忠, 牛曉泉, 孫小蓉. 用DNA倍體分析評估口腔黏膜疾病嚴重程度[J]. 臨床口腔醫學雜志, 2013, 29(6):355-357.
16. 戴一菲, 張平安. 細針穿刺細胞學檢查結合DNA倍體分析在乳腺腫塊診斷中的應用[J]. 中華臨床醫師雜志, 2013, 12(7):5587-5589.
17. 蔡元菊, 黃文波, 馬占忠. 卵巢腫瘤細胞DNA倍體和S期細胞比率的檢測及臨床意義[J]. 中華臨床醫師雜志, 2013, 7(7):3196-3197.
18. 刑應如, 胡萬發, 楊路寶. DNA倍體聯合腫瘤標志物檢測在胸腔積液診斷中的應用[J]. 中國腫瘤外科雜志, 2013, 5(3):151-155.
19. 宋曉兵. HPV感染聯合細胞DNA檢測在宮頸癌篩查中的臨床應用價值[J]. 中國實用醫藥, 2014, 9(7):90-91.

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West China Medical Journal

Clinical Value of DNA Ploidy Analysis in the Diagnosis of Benignancy and Malignancy with Bronchial Brushing or Bronchoalveolar Lavage Fluid

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