The Expression and Clinical Significance of Livin and Caspase-3 in Patients with Middle Ear Cholesteatoma_West China Medical Journal

Authors：

 LIShu-hua , DONGChuan , WANGJie

Department of Otorhinolaryngology, Mianyang Central Hospital, Mianyang, Sichuan 621000, P. R. China;

Corresponding?author：

Keywords：

Livin; Caspase-3; Immunohistochemistry; Middle ear cholesteatoma

DOI：

10.7507/1002-0179.20150311

Video：

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Objective To study the expression of inhibitor of apoptosis proteins (Livin) and aspartate-specific cysteine protease-3 (Caspase-3) in patients with middle ear cholesteatoma and its clinical significance. Method We selected 51 patients with cholesteatoma of the middle ear treated between April 2013 and March 2014 in our department to be our study subjects. Streptaridin-perosidase immunohistochemical method was adopted to detect the expression of Livin and Caspase-3 in the middle ear cholesteatoma epithelium and normal skin of external acoustic meatus. SPSS 17.0 software package was used for statistical analysis. Results The expression of Livin in cholesteatoma epithelium was significantly higher than that in the normal skin tissue of the external auditory canal (P<0.05), and the expression of Caspase-3 in cholesteatoma epithelium was significantly higher than the normal skin tissue in the external auditory canal (P<0.05). The expression of Livin and Caspase-3 in cholesteatoma epithelium was positively correlated (r=0.49, P<0.05). Conclusions There is a balance between apoptosis and inhibition of apoptosis in normal tissues, and when there is abnormal expression of Livin and Caspase-3 in normal tissues, it will cause cell apoptosis and apoptosis-inhibitory balance disorders, which causes middle ear cholesteatoma.

Citation： LIShu-hua, DONGChuan, WANGJie. The Expression and Clinical Significance of Livin and Caspase-3 in Patients with Middle Ear Cholesteatoma. West China Medical Journal, 2015, 30(6): 1083-1086. doi: 10.7507/1002-0179.20150311 Copy

1.	Fujimura M, Watanabe M, Shimizu H, et al. Expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinase (TIMP) in cerebral cavernous malformations:immunohistochemical analysis of MMP-2, -9 and TIMP-2[J]. Acta Neurochir (Wien), 2007, 149(2):179-183; discussion 183.
2.	田勇泉. 耳鼻咽喉頭頸外科學[M]. 8版. 北京:人民衛生出版社, 2013:329.
3.	Ergün S, Carls?? B, Zheng X. Apoptosis in meatal skin, cholesteatoma and squamous cell carcinoma of the ear[J]. Clin Otolaryngol Allied Sci, 1999, 24(4):280-285.
4.	潘宏光, 崔永華, 高起學. 中耳膽脂瘤分子生物學研究的新進展[J]. 臨床耳鼻咽喉科雜志, 2000, 14(5):235-237.
5.	Albino AP, Reed JA, Bogdany JK, et al. Expression of p53 protein in human middle ear cholesteatomas:pathogenetic implications[J]. Am J Otol, 1998, 19(1):30-36.
6.	Kojima H, Miyazaki H, Tanaka Y, et al. Role of Bcl-xL protein in differentiation and apoptosis of human middle ear cholesteatoma epithelium[J]. Arch Otolaryngol Head Neck Surg, 1999, 125(7): 738-742.
7.	李冬, 黃芳, 龔樹生, 等. 凋亡抑制蛋白Survivin及Bcl-2在中耳膽脂瘤上皮中的表達及意義[J]. 中國耳鼻咽喉顱底外科雜志, 2006, 12(5):334-338.
8.	Olszewska E, Chodynicki S, Chyczewski L. Apoptosis in the pathogenesis of cholesteatoma in adults[J]. Eur Arch Otorhinolaryngol, 2006, 263(5):409-413.
9.	關芳靈, 馬敏, 尚麗芳, 等. X連鎖凋亡抑制蛋白在膽脂瘤中的表達及其與細胞凋亡的關系[J]. 山西醫藥雜志, 2008, 37(6):492-494.
10.	Lin SH, Deng G, Huang Q, et al. KIAP, a novel member of the inhibitor of apoptosis protein family[J]. Biochem Biophys Res Commun, 2000, 279(3):820-831.
11.	Vucic D, Stennicke HR, Pisabarro MT, et al. ML-IAP, a novel inhibitor of apoptosis that is preferentially expressed in human melanomas[J]. Curr Biol, 2000, 10(21):1359-1366.
12.	Gazzaniga P, Gradilone A, Giuliani L, et al. Expression and prognostic significance of LIVIN, SURVIVIN and other apoptosis-related genes in the progression of superficial bladder cancer[J]. Ann Oncol, 2003, 14(1):85-90.
13.	彭利, 劉立中. 中耳膽脂瘤上皮中Livin、p73的表達與其發生關系研究[J]. 中華耳科學雜志, 2010, 8(3):315-318.
14.	楊寧, 蔣立新. 凋亡抑制蛋白Livin及Survivin在中耳膽脂瘤上皮的表達及意義[J]. 聽力學及言語疾病雜志, 2011, 19(4):356-358.
15.	伍千元, 李建軍, 李龍平. Survivin及Livin在中耳膽脂瘤組織中的表達及相關性研究[J]. 中國實用醫藥, 2011, 6(1):3-4.
16.	郝艷, 李珂, 王建亭, 等. NF-κBP65和caspase-3在中耳膽脂瘤中的表達及其臨床意義[J]. 山西醫科大學學報, 2011, 42(4):288-291.
17.	潘松林, 龔樹生, 賀紅斌. 細胞外凋亡途徑與中耳膽脂瘤細胞凋亡[J]. 中國耳鼻咽喉頭頸外科, 2007, 14(12):699-701.
18.	Bao GM, Zhang CW, Wang GX. Expressions of livin and IGF-Ⅱ in colorectalcarcinoma and their relationship[J]. Chin Germ J Clin Oncol, 2010, 9(8):470-474.
19.	Park HR, Min SK, Min K, et al. Increased expression of p63 and survivin in cholesteatomas[J]. Acta Otolaryngol, 2009, 129(3): 268-272.

1. Fujimura M, Watanabe M, Shimizu H, et al. Expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinase (TIMP) in cerebral cavernous malformations:immunohistochemical analysis of MMP-2, -9 and TIMP-2[J]. Acta Neurochir (Wien), 2007, 149(2):179-183; discussion 183.
2. 田勇泉. 耳鼻咽喉頭頸外科學[M]. 8版. 北京:人民衛生出版社, 2013:329.
3. Ergün S, Carls?? B, Zheng X. Apoptosis in meatal skin, cholesteatoma and squamous cell carcinoma of the ear[J]. Clin Otolaryngol Allied Sci, 1999, 24(4):280-285.
4. 潘宏光, 崔永華, 高起學. 中耳膽脂瘤分子生物學研究的新進展[J]. 臨床耳鼻咽喉科雜志, 2000, 14(5):235-237.
5. Albino AP, Reed JA, Bogdany JK, et al. Expression of p53 protein in human middle ear cholesteatomas:pathogenetic implications[J]. Am J Otol, 1998, 19(1):30-36.
6. Kojima H, Miyazaki H, Tanaka Y, et al. Role of Bcl-xL protein in differentiation and apoptosis of human middle ear cholesteatoma epithelium[J]. Arch Otolaryngol Head Neck Surg, 1999, 125(7): 738-742.
7. 李冬, 黃芳, 龔樹生, 等. 凋亡抑制蛋白Survivin及Bcl-2在中耳膽脂瘤上皮中的表達及意義[J]. 中國耳鼻咽喉顱底外科雜志, 2006, 12(5):334-338.
8. Olszewska E, Chodynicki S, Chyczewski L. Apoptosis in the pathogenesis of cholesteatoma in adults[J]. Eur Arch Otorhinolaryngol, 2006, 263(5):409-413.
9. 關芳靈, 馬敏, 尚麗芳, 等. X連鎖凋亡抑制蛋白在膽脂瘤中的表達及其與細胞凋亡的關系[J]. 山西醫藥雜志, 2008, 37(6):492-494.
10. Lin SH, Deng G, Huang Q, et al. KIAP, a novel member of the inhibitor of apoptosis protein family[J]. Biochem Biophys Res Commun, 2000, 279(3):820-831.
11. Vucic D, Stennicke HR, Pisabarro MT, et al. ML-IAP, a novel inhibitor of apoptosis that is preferentially expressed in human melanomas[J]. Curr Biol, 2000, 10(21):1359-1366.
12. Gazzaniga P, Gradilone A, Giuliani L, et al. Expression and prognostic significance of LIVIN, SURVIVIN and other apoptosis-related genes in the progression of superficial bladder cancer[J]. Ann Oncol, 2003, 14(1):85-90.
13. 彭利, 劉立中. 中耳膽脂瘤上皮中Livin、p73的表達與其發生關系研究[J]. 中華耳科學雜志, 2010, 8(3):315-318.
14. 楊寧, 蔣立新. 凋亡抑制蛋白Livin及Survivin在中耳膽脂瘤上皮的表達及意義[J]. 聽力學及言語疾病雜志, 2011, 19(4):356-358.
15. 伍千元, 李建軍, 李龍平. Survivin及Livin在中耳膽脂瘤組織中的表達及相關性研究[J]. 中國實用醫藥, 2011, 6(1):3-4.
16. 郝艷, 李珂, 王建亭, 等. NF-κBP65和caspase-3在中耳膽脂瘤中的表達及其臨床意義[J]. 山西醫科大學學報, 2011, 42(4):288-291.
17. 潘松林, 龔樹生, 賀紅斌. 細胞外凋亡途徑與中耳膽脂瘤細胞凋亡[J]. 中國耳鼻咽喉頭頸外科, 2007, 14(12):699-701.
18. Bao GM, Zhang CW, Wang GX. Expressions of livin and IGF-Ⅱ in colorectalcarcinoma and their relationship[J]. Chin Germ J Clin Oncol, 2010, 9(8):470-474.
19. Park HR, Min SK, Min K, et al. Increased expression of p63 and survivin in cholesteatomas[J]. Acta Otolaryngol, 2009, 129(3): 268-272.

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