A primary research of the thermal damage of rabbit kidney by domestic holmium laser in surgery_West China Medical Journal

Authors：

GONG Chunyu ¹ , LI Li ¹ , QU Rui ¹ , YANG Luo ¹ , FENG Yangyang ¹ , DENG Huizhuo ¹ , LUO Xiaorong ¹ ,  YANG Jing ²

1. Department of Surgery, No. 4 West China Teaching Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;
2. Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding?author：

YANG Jing, Email: macrobun@163.com

Keywords：

Holmium laser; Kidney; Ureter; Thermal damage; Rabbit

DOI：

10.7507/1002-0179.201601009

Video：

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ObjectiveTo observe the effect of thermal damage in the fresh isolated kidneys of New Zealand white rabbits caused by domestic holmiumlaser. MethodsIn the operation room (constant temperature 22℃, humidity 60%), Guangdong electric (POTENT) domestic holmium laser equipment was chosen. The fresh isolated kidney of New Zealand white rabbit was put into a disposable sterilized syringe with 50 mL normal saline, then the holmium laser optical fiber (550 μm) was put into it, and the temperature of normal saline was measured by a mercury thermometer. The parameters of holmium laser were setted as frequency 20 Hz, energy 2 J, stimulating time 15 seconds, intermittent time 5 seconds, which was repeated. The temperature was measured 2, 5 and 7 minutes after stimulation. Then the kidney was dissected, phtographed, haematoxylin-eosin stained, and pathologically examined.ResultsAt the 2nd minute of stimulation, the temperature of normal saline in the syringe increased from 22℃ to 38℃; the cortex and medulla of rabbit kidney were ruddy, and the cortex, medulla and ureter were almost normal in pathological section. At the 5-minute point, the temperature increased to 57℃, and the cortex turned to be white, while the medulla remained ruddy, but the demarcation between the cortex and medulla was not very clear. At the 7th minute, the temperature was 78℃, and the cortex and medulla were both white and solidification with no clear demarcation. Pathological examination showed severe degeneration and necrosis of glomerulus and renal tubule.ConclusionCommon power of domestic holmium laser would produce increasing thermal damage, which may cause tissue damage in the human body when the temperature increases above 50℃.

Citation： GONG Chunyu, LI Li, QU Rui, YANG Luo, FENG Yangyang, DENG Huizhuo, LUO Xiaorong, YANG Jing. A primary research of the thermal damage of rabbit kidney by domestic holmium laser in surgery. West China Medical Journal, 2017, 32(7): 984-987. doi: 10.7507/1002-0179.201601009 Copy

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10.	李良菊, 朱泉玉, 胡海燕. 經輸尿管鏡鈥激光碎石日間手術的安全及質量評價. 海南醫學, 2017, 28(1): 141-143.
11.	崔增林, 張峰, 卞建強. 輸尿管鏡聯合鈥激光碎石治療嵌頓性輸尿管上段結石體會. 河南外科學雜志, 2016, 22(2): 112-113.
12.	呂國強, 宋東奎, 郝凱. 輸尿管鏡鈥激光碎石術治療老年輸尿管結石療效及對腎功能的影響觀察. 基層醫學論壇, 2016, 20 (35): 4950-4951.
13.	羅魁德, 殷凡榮. 經輸尿管鈥激光碎石術治療輸尿管結石 100 例臨床療效. 世界最新醫學文摘, 2106, 16(12): 199-120.
14.	Grasso M, Fraiman M, Levine M. Ureteropyeloscopic diagnosis and treatment of upper urinary tract urothelial malignancies. Urology, 1999, 54(2): 240-246.
15.	Ng EY, Tan HM, Ooi EH. Boundary element method with bioheat equation for skin burn injury. Burns, 2009, 35(7): 987-997.
16.	龔云輝. 輸尿管鏡彈道與鈥激光碎石治療輸尿管結石致腎功能衰竭的臨床分析. 中國醫藥指南, 2016, 4(26): 194-195.

1. 孫穎浩. 鈥激光在泌尿外科中的應用. 中國現代手術學雜志, 2003, 7(5): 331-334.
2. 羅建仕. 鈥激光技術在泌尿系結石中的應用. 微創醫學, 2006, 1(2): 115-117.
3. 劉慶. 多用途鈥激光: 泌尿外科的新技術. 醫療設備信息, 2005, 20(1): 27-28.
4. 王坤杰, 李虹. 輸尿管軟鏡聯合鈥激光在特殊上尿路結石治療中的運用. 現代泌尿外科雜志, 2015, 20(11): 761-763.
5. 鎖錦江. 輸尿管鏡下鈥激光治療輸尿管結石伴息肉療效分析. 浙江臨床醫學, 2017, 19(2): 296-297.
6. 俞宇宙, 胡明進, 朱洪翔, 等. 微創經皮腎鏡鈥激光碎石取石術在腎結石中的應用效果分析. 臨床醫藥文獻雜志, 2016, 3(43): 8565.
7. 袁磊, 馬永, 婁騰飛, 等. 改良方法在輸尿管鏡鈥激光治療輸尿管結石中的應用. 淮海醫藥, 2016, 34(5): 545-546.
8. 粟周全, 茍興連, 杜龍. 輸尿管鈥激光碎石術和微創經皮腎鏡碎石術治療輸尿管上段結石療效觀察. 陜西醫學雜志, 2014, 43(4): 443-444.
9. 張俊巖, 王德剛, 王文剛. 斜臥位經皮腎輸尿管鏡下鈥激光碎石術治療復雜性腎結石. 中國醫藥指南, 2017, 15(2): 153-154.
10. 李良菊, 朱泉玉, 胡海燕. 經輸尿管鏡鈥激光碎石日間手術的安全及質量評價. 海南醫學, 2017, 28(1): 141-143.
11. 崔增林, 張峰, 卞建強. 輸尿管鏡聯合鈥激光碎石治療嵌頓性輸尿管上段結石體會. 河南外科學雜志, 2016, 22(2): 112-113.
12. 呂國強, 宋東奎, 郝凱. 輸尿管鏡鈥激光碎石術治療老年輸尿管結石療效及對腎功能的影響觀察. 基層醫學論壇, 2016, 20 (35): 4950-4951.
13. 羅魁德, 殷凡榮. 經輸尿管鈥激光碎石術治療輸尿管結石 100 例臨床療效. 世界最新醫學文摘, 2106, 16(12): 199-120.
14. Grasso M, Fraiman M, Levine M. Ureteropyeloscopic diagnosis and treatment of upper urinary tract urothelial malignancies. Urology, 1999, 54(2): 240-246.
15. Ng EY, Tan HM, Ooi EH. Boundary element method with bioheat equation for skin burn injury. Burns, 2009, 35(7): 987-997.
16. 龔云輝. 輸尿管鏡彈道與鈥激光碎石治療輸尿管結石致腎功能衰竭的臨床分析. 中國醫藥指南, 2016, 4(26): 194-195.

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A primary research of the thermal damage of rabbit kidney by domestic holmium laser in surgery

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