Application of Sysmex XT-4000i hematology analyzer in cell count and cell differential count of body-fluid samples_West China Medical Journal

Authors：

CHEN Jiao , HUANG Qian , DING Bin , MAO Zhigang ,  SU Jun

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding?author：

SU Jun, Email: sjhuaxi1667@163.com

Keywords：

Auto-hematology analyzer; Pleural effusion; Ascitic fluid; Cerebrospinal fluid; Cell count; Cell differential count

DOI：

10.7507/1002-0179.201601134

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To evaluate the value of Sysmex XT-4000i hematology analyzer in its body-fluid mode in cell count and cell differential count of pleural effusion, ascites and cerebrospinal fluid samples. Methods A total of 95 pleural effusion, ascites and cerebrospinal fluid samples were collected from patients hospitalized between May and September 2015. The samples were tested by Sysmex XT-4000i hematology analyzer (instrument method) and modified Neubauer hemocytometer (manual method) for cell count, and the results of them were compared and analyzed. Results The instrument method and the manual method had a good consistency in nuclear cell count and erythrocyte count (kappa=0.965,P< 0.001; kappa=0.988,P<0.001). There was no significant difference in the count of mononuclear cells (P> 0.05). However, there was a significant difference in the count of multiple nuclear cells (P<0.05). Conclusions Hematology analyzer in its body-fluid mode may replace manual method in cell count of pleural effusion, ascites and cerebrospinal fluids for its high precision, high efficiency and easy operation. However, cell differential count of this method needs microscopic examination assistance.

Citation： CHEN Jiao, HUANG Qian, DING Bin, MAO Zhigang, SU Jun. Application of Sysmex XT-4000i hematology analyzer in cell count and cell differential count of body-fluid samples. West China Medical Journal, 2017, 32(4): 563-566. doi: 10.7507/1002-0179.201601134 Copy

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2.	Ndhaus LM. Body fluid cell counts by automated methods. Clin Lab Med, 2015, 35(1): 93-103.
3.	Sandhaus LM, Ciarlini P, Kidric D, et al. Automated cerebrospinal fluid cell counts using the Sysmex XE-5000: is it time for new reference ranges?. Am J Clin Pathol, 2010, 134(5): 734-738.
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8.	張金花, 胡娟, 李君安. Sysmex XE-5000 全自動血細胞分析儀體液模式對胸、腹腔積液白細胞計數與分類計數的結果分析. 國際檢驗醫學雜志, 2015, 36(1): 45-46.
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10.	夏永泉, 徐學靜, 王賢. Sysmex XE-5000 血液分析儀體液模式白細胞計數準確性的探討. 國際檢驗醫學雜志, 2013, 34(23): 3228-3229.
11.	Genc S, Dervisoglu E, Omer D, et al. Evaluation of cell counting in body fluids: comparison of two automated hematology analyzers with manual microscopy. Clin Lab, 2016, 62(12): 2449-2453.
12.	Fleming C, Russcher H, Brouwer R, et al. Evaluation of Sysmex XN-1000 high-sensitive analysis (hsa) research mode for counting and differentiating cells in cerebrospinal fluid. Am J Clin Pathol, 2016, 145(3): 299-307.
13.	Buoro S, Seghezzi M, Mecca T, et al. Evaluation of Mindray BC-6800 body fluid mode for automated cerebrospinal fluid cell counting. Clin Chem Lab Med, 2016, 54(11): 1799-1810.
14.	郭唯, 高春玲, 劉娜. SysmexUF-500i 與 XS-500i 在體液細胞計數中的應用評價. 臨床檢驗雜志, 2013, 20(21): 98-99.
15.	張慧, 李琳, 續薇. Sysmex XE-5000 全自動血細胞分析儀體液模式與手工法體液細胞計數及分類的比較. 國際檢驗醫學雜志, 2015, 36(22): 3238-3239, 3242.
16.	Yang D, Zhou Y, Chen B. Performance evaluation and result comparison of the automated hematology analyzers Abbott CD 3700, Sysmex XE 2100 and Coulter LH 750 for cell counts in serous fluids. Clin Chim Acta, 2013, 419: 113-118.
17.	Liang X, Chen J, Xiao X, et al. Automated cell analysis of cerebrospinal fluid with XE-5000. Clin Lab, 2014, 60(11): 1785-1793.

1. Clinical and Laboratory Standards Institute. H26-A2 Validation, verification and quality assurance of automated hematologyanalyzers, 2010.
2. Ndhaus LM. Body fluid cell counts by automated methods. Clin Lab Med, 2015, 35(1): 93-103.
3. Sandhaus LM, Ciarlini P, Kidric D, et al. Automated cerebrospinal fluid cell counts using the Sysmex XE-5000: is it time for new reference ranges?. Am J Clin Pathol, 2010, 134(5): 734-738.
4. 葉應嫵, 王毓三, 申子瑜. 全國臨床檢驗操作規程. 3版. 南京: 東南大學出版社, 2006: 312-313.
5. 戴蕓, 楊輝, 樂華文. 胸腹水有核細胞計數自動化檢測探討. 國際檢驗醫學雜志, 2013, 34(7): 859, 863.
6. 李玲, 劉文康, 李博, 等. 多臺全自動血細胞分析儀檢測結果的比對研究. 現代檢驗醫學雜志, 2012, 27(3): 119-121.
7. 陳群, 聶署萍, 張志城, 等. 全自動血液分析儀檢測特殊體液有核細胞與手工法的結果比對. 國際檢驗醫學雜志, 2014, 35(21): 2949-2950.
8. 張金花, 胡娟, 李君安. Sysmex XE-5000 全自動血細胞分析儀體液模式對胸、腹腔積液白細胞計數與分類計數的結果分析. 國際檢驗醫學雜志, 2015, 36(1): 45-46.
9. 白雪麗. XT-4000i 多功能全自動血細胞分析儀在體液細胞檢測中與手工法的應用比較. 中國醫療設備, 2012, 27(1): 75-76.
10. 夏永泉, 徐學靜, 王賢. Sysmex XE-5000 血液分析儀體液模式白細胞計數準確性的探討. 國際檢驗醫學雜志, 2013, 34(23): 3228-3229.
11. Genc S, Dervisoglu E, Omer D, et al. Evaluation of cell counting in body fluids: comparison of two automated hematology analyzers with manual microscopy. Clin Lab, 2016, 62(12): 2449-2453.
12. Fleming C, Russcher H, Brouwer R, et al. Evaluation of Sysmex XN-1000 high-sensitive analysis (hsa) research mode for counting and differentiating cells in cerebrospinal fluid. Am J Clin Pathol, 2016, 145(3): 299-307.
13. Buoro S, Seghezzi M, Mecca T, et al. Evaluation of Mindray BC-6800 body fluid mode for automated cerebrospinal fluid cell counting. Clin Chem Lab Med, 2016, 54(11): 1799-1810.
14. 郭唯, 高春玲, 劉娜. SysmexUF-500i 與 XS-500i 在體液細胞計數中的應用評價. 臨床檢驗雜志, 2013, 20(21): 98-99.
15. 張慧, 李琳, 續薇. Sysmex XE-5000 全自動血細胞分析儀體液模式與手工法體液細胞計數及分類的比較. 國際檢驗醫學雜志, 2015, 36(22): 3238-3239, 3242.
16. Yang D, Zhou Y, Chen B. Performance evaluation and result comparison of the automated hematology analyzers Abbott CD 3700, Sysmex XE 2100 and Coulter LH 750 for cell counts in serous fluids. Clin Chim Acta, 2013, 419: 113-118.
17. Liang X, Chen J, Xiao X, et al. Automated cell analysis of cerebrospinal fluid with XE-5000. Clin Lab, 2014, 60(11): 1785-1793.

West China Medical Journal

Application of Sysmex XT-4000i hematology analyzer in cell count and cell differential count of body-fluid samples

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