Epilepsy model rat hippocampus dentate gyrus neurons apoptosis and the significance of apoptosis-inducing factor and Caspase-3 joint expression_West China Medical Journal

Authors：

DING Chunxiao ¹ , LIU Xiaohu ¹ , QI Yue ¹ , QIAO Jujiu ¹ ,  LI Jianhua ²

1. Department of Pathology, Chinese Medicine Research Institute of Liaoning Province, Shenyang, Liaoning 110034, P. R. China;
2. Division and Department of Pathology, the First Affiliated Hospital, Chinses Medical University, Shenyang, Liaoning 110001, P. R. China;

Corresponding?author：

LI Jianhua, Email: LIJH@mail.cmu.edu.cn

Keywords：

Pentrolone; Apoptosis; Nissl staining; Immunohistochemistry; Caspase-3; Apoptosis-inducing factor; Epilepsy

DOI：

10.7507/1002-0179.201604034

Video：

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Objective To observe the apoptosis of hippocampal dentate gyrus in rats with pentrolone model of epilepsy, and detect the significance of apoptosis-inducing factor (AIF) and Caspase-3 joint expression. Methods Twelve Wistar rats were randomly divided into model group and control group with 6 in each. Intraperitoneal injection of pentrolone was carried out for rats in the model group, while physiological saline was injected in rats of the control group. After the model was successfully established, we observed the Nissl staining results of hippocampus dentate gyrus tissue in the rats as well as the expression of AIF and Caspase-3 protein in both groups. Results The single visual nerve cell counts of the control group and the model group under 400-times magnification were respectively 99.76±11.89 and 78.69±10.94; Caspase-3 gray values of the two groups were respectively 154.81±16.06 and 131.65±16.81; and AIF protein gray values were respectively 173.09±9.57 and 158.34±6.33. All the above differences were statistically significant (P<0.05). Conclusion The two apoptotic pathways through Caspase-3 dependent on Caspase and AIF independent of Caspase are both involved in the neuronal apoptosis induced by epilepsy.

Citation： DING Chunxiao, LIU Xiaohu, QI Yue, QIAO Jujiu, LI Jianhua. Epilepsy model rat hippocampus dentate gyrus neurons apoptosis and the significance of apoptosis-inducing factor and Caspase-3 joint expression. West China Medical Journal, 2017, 32(4): 550-553. doi: 10.7507/1002-0179.201604034 Copy

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11.	馮建利, 姚博, 閆鵬, 等. 大鼠局灶性腦缺血再灌注后凋亡誘導因子的表達及其與神經細胞凋亡的關系. 中國老年學雜志, 2007, 27(20): 1955-1957.
12.	李巧云, 張海華, 游仁芳. Caspase-3 表達及神經元凋亡的影響. 南昌大學學報, 2013, 53(10): 11-14.
13.	高維娟, 張霞, 劉莎莎. 大鼠腦缺血再灌注后海馬 CA1 區 Caspase-3 的表達及細胞凋亡的動態變化. 中國老年學雜志, 2013, 33(24): 6202-6204.
14.	楊宜承, 趙薇, 曾常茜, 等. 雷公藤內酯對海人酸致癇大鼠神經元 caspase3 和 caspase9 蛋白表達的影響. 遼寧中醫藥大學學報, 2013, 15(2): 48-50.
15.	劉小虎, 向邵杰, 齊越, 等. 戊四唑急性癲癇模型海馬病理組織的病理變化. 中國藥理學通報, 2015, 31(4): 514-518.
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17.	Izzi L, Attisano L. Ubiquitin-dependent regulation of TGF beta signaling in cancer. Neoplasia, 2006, 8(8): 677-688.

1. ?Schubert M, Siegmund H, Pape HC, et al. Kindling-induced changes in plasticity of the rat amygdala and hippocampus. Learn Mem, 2005, 12(5): 520-526.
2. 秦曉云, 竇長武, 張占普, 等. 動態腦電圖監測癲癇患者的臨床意義. 西部醫學, 2016, 28(3): 358-360, 364.
3. 楊帆, 楊永清. 癲癇與腦神經元損傷. 醫學綜述, 2009, 15(19): 2881-2884.
4. 李應宏, 莫燁, 李勁梅, 等. 耐藥性癲癇患者腦內 Nurr1 基因及蛋白產物的表達. 西部醫學, 2015, 27(1): 5-8, 12.
5. 王守權, 隋鴻錦, 宮瑾, 等. 戊四氮急性癲癇模型齒狀回分子層突觸的可塑性變化. 解剖學雜志, 2004, 27(3): 288-290.
6. 王玢, 遲華基, 袁方曜. 中藥復方對戊四氮致癇大鼠海馬神經元 caspase-3 表達的影響. 山東教育學院學報, 2010, 25(6): 39-42.
7. 梁義, 楊忠, 魏璐, 等. 戊四氮點燃 SD 大鼠制作慢性癲癇模型中不同注射劑量的對比研究. 武警醫學, 2007, 18(4): 264-266.
8. Shibeeb O, Wood JP, Casson RJ, et al. Effects of a conventional photocoagulator and a 3-ns pulse laser on preconditioning responses and retinal ganglion cell survival after optic nerve crush. Exp Eye Res, 2014, 127(127): 77-90.
9. Dhaher R, Damisah EC, Wang H, et al. 5-aminovaleric acid suppresses the development of severe seizures in the methionine sulfoximine model of mesial temporal lobe epilepsy. Neurobiol Dis, 2014, 67(67): 18-23.
10. 張瑋, 韓寧, 胡金鳳, 等. 化合物 IMM-H004 對大鼠急性全腦缺血/再灌注損傷的保護作用. 中國藥理學通報, 2013, 29(12): 1654-1659.
11. 馮建利, 姚博, 閆鵬, 等. 大鼠局灶性腦缺血再灌注后凋亡誘導因子的表達及其與神經細胞凋亡的關系. 中國老年學雜志, 2007, 27(20): 1955-1957.
12. 李巧云, 張海華, 游仁芳. Caspase-3 表達及神經元凋亡的影響. 南昌大學學報, 2013, 53(10): 11-14.
13. 高維娟, 張霞, 劉莎莎. 大鼠腦缺血再灌注后海馬 CA1 區 Caspase-3 的表達及細胞凋亡的動態變化. 中國老年學雜志, 2013, 33(24): 6202-6204.
14. 楊宜承, 趙薇, 曾常茜, 等. 雷公藤內酯對海人酸致癇大鼠神經元 caspase3 和 caspase9 蛋白表達的影響. 遼寧中醫藥大學學報, 2013, 15(2): 48-50.
15. 劉小虎, 向邵杰, 齊越, 等. 戊四唑急性癲癇模型海馬病理組織的病理變化. 中國藥理學通報, 2015, 31(4): 514-518.
16. Nicholson DW, Ali A, Thornberry NA, et al. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature, 1995, 376(6535): 37-43.
17. Izzi L, Attisano L. Ubiquitin-dependent regulation of TGF beta signaling in cancer. Neoplasia, 2006, 8(8): 677-688.

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Epilepsy model rat hippocampus dentate gyrus neurons apoptosis and the significance of apoptosis-inducing factor and Caspase-3 joint expression

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