The relationship between the ratio of expression of heat shock protein 70/ glial fibrillary acidic protein and post-injury interval after concussion of brain in rats_West China Medical Journal

Authors：

DAI Hao ¹ , CHEN Bo ² , JIN Wei ¹ ,  LIU Min ¹

1. Department of Forensic Pathology, West China School of Basic and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P. R. China;
2. Criminal Science and Technology Office, Jianyang Public Security Bureau, Jianyang, Sichuan 641499, P. R. China;

Corresponding?author：

LIU Min, Email: min8liu@hotmail.com

Keywords：

Concussion of Brain; Post-injury interval; Heat shock protein 70; Glial fibrillary acidic protein; The ratio of percentage of positive cells; Rat

DOI：

10.7507/1002-0179.201611100

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Objectives To detect expressions of heat shock protein 70 (HSP70) and glial fibrillary acidic protein (GFAP) , and to estimate the post-injury interval after concussion of brain via the ratios of percentage of HSP70/GFAP-positive cells. Methods We established a brain concussion model of rat. Tissue levels of HSP70 and GFAP were determined by immunohistochemical staining at different time points after injury. Finally, the relationship between the ratio of percentage of HSP70/GFAP-positive cells and the post-injury interval was measured. Results The ratio of percentage of positive cells (increased from 7.15 to 11.73) and the percentage of HSP70-positive cells (P<0.05, compared with control group) increased, and the percentage of GFAP-positive cells did not change remarkably (P<0.05, compared with control group); the post-injury interval was between 0.5 hour and 3 hours. High ratio (>6.66) and high percentage of HSP70 and GFAP-positive cells (P<0.05, compared with control group) indicated the post-injury interval was between 3 and 12 hours. A low ratio (<6.66) and high percentage of HSP70 and GFAP-positive cells (P<0.05, compared with control group) suggested that the post-injury interval was later than 12 hours. Conclusion By analyzing the variation rule of the ratio of percentage positive cells after brain concussion, the post-injury interval after concussion of brain could be estimated.

Citation： DAI Hao, CHEN Bo, JIN Wei, LIU Min. The relationship between the ratio of expression of heat shock protein 70/ glial fibrillary acidic protein and post-injury interval after concussion of brain in rats. West China Medical Journal, 2017, 32(12): 1890-1894. doi: 10.7507/1002-0179.201611100 Copy

1.	Olczak M, Niderla-Bielińska J, Kwiatkowska M, et al. Tau protein (MAPT) as a possible biochemical marker of traumatic brain injury in postmortem examination. Forensic Sci Int, 2017, 280: 1-7.
2.	Defazio MV, Rammo RA, Robles JR, et al. The potential utility of blood-derived biochemical markers as indicators of early clinical trends following severe traumatic brain injury. World Neurosurg, 2014, 81(1): 151-158.
3.	胡玉蓮, 許光亞, 劉敏. 大鼠腦挫傷后腦 C-FOS、AQP-4 表達與損傷時間的關系. 中國法醫學雜志, 2015, 30(1): 24-28.
4.	陳銳, 嚴治, 胡玲, 等. 大鼠腦震蕩模型建立法的改良和評價. 廣東醫學院學報, 2010, 28(4): 362-363.
5.	周亦武, 張益鵠, 鄧偉年, 等. 87 例大腦及腦干挫傷 HSP70 變化的研究. 中國法醫學雜志, 2005, 20(4): 209-212.
6.	Zhang J, Tao DQ, Zhao H, et al. Expression of Hsp70 and caspase-3 in rabbits after severe traumatic brain injury. Chin J Traumatol, 2012, 15(6): 338-341.
7.	Brenner M. Role of GFAP in CNS injuries. Neurosci Lett, 2014, 565(17): 7-13.
8.	李玉紅, 閆平, 孟曉萍, 等. 實驗性大鼠顱腦損傷后 GFAP 的表達研究. 傷殘醫學雜志, 2004, 12(2): 1-3.
9.	Stewart A, Tekes A, Huisman TA, et al. Glial fibrillary acidic protein as a biomarker for periventricular white matter injury. Am J Obstet Gynecol, 2013, 209(1): 27.e1-27.e7.
10.	Stetler RA, Gan Y, Zhang W, et al. Heat shock proteins: cellular and molecular mechanisms in the central nervous system. Prog Neurobiol, 2010, 92(2): 184-211.
11.	Bunch H. RNA polymeraseⅡpausing and transcriptional regulation of the HSP70 expression. Eur J Cell Biol, 2017, 96(8): 739-745.
12.	曾麗容, 鄭定容. 急性顱腦損傷腦脊液 IL-19、IL-18 與熱休克蛋白-70 變化及意義. 中華臨床醫師雜志: 電子版, 2015, 9(4): 684-686.
13.	金偉, 夏勇軍, 李雪榕, 等. 大鼠腦震蕩傷后腦外主要器官熱休克蛋白 70、C-fos 表達比值與損傷時間變化的關系研究. 華西醫學, 2017, 32(10): 1539-1543.
14.	陳銳, 余彬華, 胡玲, 等. 大鼠腦震蕩傷后腦組織 HSP70、bFGF 及TGF-β₁ 表達變化. 法醫學雜志, 2009, 25(4): 249-253.
15.	郝芳芳, 賀婕, 孫志華, 等. 蛛網膜下腔出血患者 GFAP、S100B 的表達與預后相關性研究. 檢驗醫學與臨床, 2017, 14(20): 3061-3063.
16.	Liu MD, Luo P, Wang ZJ, et al. Changes of serum Tau, GFAP, TNF-α and malonaldehyde after blast-related traumatic brain injury. Chin J Traumatol, 2014, 17(6): 317-322.
17.	Singh K, Trivedi R, Devi MM, et al. Longitudinal changes in the DTI measures, anti-GFAP expression and levels of serum inflammatory cytokines following mild traumatic brain injury. Exp Neurol, 2016, 275(Pt 3): 427-435.

1. Olczak M, Niderla-Bielińska J, Kwiatkowska M, et al. Tau protein (MAPT) as a possible biochemical marker of traumatic brain injury in postmortem examination. Forensic Sci Int, 2017, 280: 1-7.
2. Defazio MV, Rammo RA, Robles JR, et al. The potential utility of blood-derived biochemical markers as indicators of early clinical trends following severe traumatic brain injury. World Neurosurg, 2014, 81(1): 151-158.
3. 胡玉蓮, 許光亞, 劉敏. 大鼠腦挫傷后腦 C-FOS、AQP-4 表達與損傷時間的關系. 中國法醫學雜志, 2015, 30(1): 24-28.
4. 陳銳, 嚴治, 胡玲, 等. 大鼠腦震蕩模型建立法的改良和評價. 廣東醫學院學報, 2010, 28(4): 362-363.
5. 周亦武, 張益鵠, 鄧偉年, 等. 87 例大腦及腦干挫傷 HSP70 變化的研究. 中國法醫學雜志, 2005, 20(4): 209-212.
6. Zhang J, Tao DQ, Zhao H, et al. Expression of Hsp70 and caspase-3 in rabbits after severe traumatic brain injury. Chin J Traumatol, 2012, 15(6): 338-341.
7. Brenner M. Role of GFAP in CNS injuries. Neurosci Lett, 2014, 565(17): 7-13.
8. 李玉紅, 閆平, 孟曉萍, 等. 實驗性大鼠顱腦損傷后 GFAP 的表達研究. 傷殘醫學雜志, 2004, 12(2): 1-3.
9. Stewart A, Tekes A, Huisman TA, et al. Glial fibrillary acidic protein as a biomarker for periventricular white matter injury. Am J Obstet Gynecol, 2013, 209(1): 27.e1-27.e7.
10. Stetler RA, Gan Y, Zhang W, et al. Heat shock proteins: cellular and molecular mechanisms in the central nervous system. Prog Neurobiol, 2010, 92(2): 184-211.
11. Bunch H. RNA polymeraseⅡpausing and transcriptional regulation of the HSP70 expression. Eur J Cell Biol, 2017, 96(8): 739-745.
12. 曾麗容, 鄭定容. 急性顱腦損傷腦脊液 IL-19、IL-18 與熱休克蛋白-70 變化及意義. 中華臨床醫師雜志: 電子版, 2015, 9(4): 684-686.
13. 金偉, 夏勇軍, 李雪榕, 等. 大鼠腦震蕩傷后腦外主要器官熱休克蛋白 70、C-fos 表達比值與損傷時間變化的關系研究. 華西醫學, 2017, 32(10): 1539-1543.
14. 陳銳, 余彬華, 胡玲, 等. 大鼠腦震蕩傷后腦組織 HSP70、bFGF 及TGF-β₁ 表達變化. 法醫學雜志, 2009, 25(4): 249-253.
15. 郝芳芳, 賀婕, 孫志華, 等. 蛛網膜下腔出血患者 GFAP、S100B 的表達與預后相關性研究. 檢驗醫學與臨床, 2017, 14(20): 3061-3063.
16. Liu MD, Luo P, Wang ZJ, et al. Changes of serum Tau, GFAP, TNF-α and malonaldehyde after blast-related traumatic brain injury. Chin J Traumatol, 2014, 17(6): 317-322.
17. Singh K, Trivedi R, Devi MM, et al. Longitudinal changes in the DTI measures, anti-GFAP expression and levels of serum inflammatory cytokines following mild traumatic brain injury. Exp Neurol, 2016, 275(Pt 3): 427-435.

West China Medical Journal

The relationship between the ratio of expression of heat shock protein 70/ glial fibrillary acidic protein and post-injury interval after concussion of brain in rats

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