The impact of Liuhedan on interleukin-1β and tumor necrosis factor-alpha during infective wound healing in rabbits_West China Medical Journal

Authors：

Lü Changyao , WU Jing ,  HE Hongbo

Department of Integrated Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding?author：

HE Hongbo, Email: 18980601615@163.com

Keywords：

Liuhedan; Wound healing; Interleukin-1β; Tumor necrosis factor-α

DOI：

10.7507/1002-0179.201703100

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To explore the mechanism of Liuhedan in promoting wound healing through applying Liuhedan to the infective wounds of New Zealand white rabbits. Methods A total of forty New Zealand white rabbit models of infective wounds were established after anesthesia. Five circular infective incisions were generated on the back of each rabbit, with a diameter of 2 cm. Five wounds of each rabbit were assigned respectively to the control group, model group, traditional Chinese medicine (TCM) group (Oleum Lithospermum), Western medicine group (calcium alginat), and treatment group (Liuhedan). Wound dressings were performed every day since postoperative day 1. Ten rabbits were selected randomly to be euthanized on postoperative day 3, 7, 14 and 21, respectively. Each specimen was divided into two parts. One was used for detecting interleukin-1β (IL-1β) by enzyme-linked immunosorbent assay, and the other was used for detecting tumor necrosis factor-α (TNF-α) by immunocytochemistry. Results On postoperative day 3 and 7, groups with the expression of IL-1β from low to high were respectively the control group, the treatment group, the Western medicine group, the TCM group, and the model group [postoperative day 3: (680.81±185.53), (1 028.67±205.57), (1 278.67±251.15), (1 449.86±230.74), (1 544.62±371.77) pg/mL; postoperative day 7: (1 024.43±239.94), (1 333.57±257.31), (1 635.14±222.40), (1 784.71±323.85), (1 953.29±324.78) pg/mL], and all the differences among the groups were significant (P<0.05); On postoperative day 14, groups with the expression of IL-1β from low to high were respectively the treatment group, the control group, the Western medicine group, the TCM group, and the model group [(908.71±108.61), (978.57±161.75), (1 120.43±265.39), (1 129.71±298.06), (1 191.14±234.92) pg/mL], and all the differences among groups were significant (P<0.05) except the difference between the Western medicine group and the TCM group (P>0.05); On postoperative day 21, the expression of IL-1β in the control group, the model group, the TCM group, and the Western medicine group was (487.19±121.80), (496.35±102.15), (500.31±139.34), (499.08±120.67) pg/mL, respectively, with no significant differences among the groups (P>0.05), which were all higher than that in the treatment group [(398.62±102.93) pg/mL] with significant difference (P<0.05). The expression of TNF-α in the model group was significantly greater than those in the other groups. The expression of TNF-α in the treatment group and Western medicine group was significantly lower compared with the model group. The expression of TNF-α in the TCM group was stronger compared with those in the treatment group and the Western medicine group. Conclusion Liuhedan can specifically suppress the expressions of IL-1β and TNF-α in the treatment of infective wounds, decrease the release of inflammatory factor and promote the healing.

Citation： Lü Changyao, WU Jing, HE Hongbo. The impact of Liuhedan on interleukin-1β and tumor necrosis factor-alpha during infective wound healing in rabbits. West China Medical Journal, 2017, 32(10): 1544-1550. doi: 10.7507/1002-0179.201703100 Copy

1.	熊艷艷, 張劉娜, 陳本會. 外用六合丹濕敷治療急性嵌頓痔水腫的療效觀察. 華西醫學, 2016, 31(5): 955-957.
2.	李輝香, 陳本會. 中藥六合丹外敷對混合痔外剝內扎術后水腫的效果評價. 華西醫學, 2015, 30(7): 1280-1281.
3.	李黎, 邱生林, 岳曉燕. 六合丹外敷治療流行性腮腺炎患兒腮腺及睪丸腫大的療效觀察及護理. 2016, 38(1): 113-116.
4.	葉靜, 黎貴湘. 六合丹外敷治療早期哺乳期乳腺炎的療效觀察. 現代中西醫結合雜志, 2013, 22(2): 185-186.
5.	中華醫學會消化病學分會, 房靜遠, 劉文忠, 等. 中國慢性胃炎共識意見(2012 年, 上海). 中華消化雜志, 2013, 33(1): 5-16.
6.	Liu D, Huang Y, Chen B, et al. Activation of mammalian target of rapamycin pathway confers adverse outcome in nonsmall cell lung carcinoma. Cancer, 2011, 117(16): 3763-3773.
7.	李芬, 李巍, 舒勤. TGF-β 與燒傷創面愈合. 創傷外科雜志, 2016, 18(12): 756-761.
8.	Arend WR. The balance between IL-1 and IL-1Ra in disease. Cytokine Growth Factor Rev, 2002, 13(4/5): 323-340.
9.	石磊. 白介素 1 與幽門螺旋桿菌研究進展. 河北醫學, 2010, 16(10): 1252-1254.
10.	Kapadi AS, Lee J, Torre-Amione G, et al. Tumor necrosis factor-alpha gene and protein expression in adult feline myocardium after endotoxin administration. J Clin Invest, 1995, 96(2): 1042-1052.
11.	秦建中, 秦儉, 王光. TNF-α 對表皮細胞增殖的影響. 臨床皮膚科雜志, 1995(1): 3-4.
12.	Yew TL, Hung YT, Li HY, et al. Enhancement of wound healing by human multipotent stromal cell conditioned medium: the paracrine factors and p38 MAPK activation. Cell Transplant, 2011, 20(5): 693-706.
13.	吳洋, 周樹夏, 苗林. 槍彈傷口軟組織中 IL-6、IL-8、TNF-α 變化的免疫組化研究. 第二軍醫大學學報, 2001, 22(12): 1117-1118.
14.	彭小航, 黃偉, 竹林, 等. 微米六合丹外敷對急性胰腺炎大鼠氧化應激及炎癥反應水平的影響. 四川大學學報: 醫學版, 2013, 44(6): 978-981.
15.	彭小航, 趙先林, 劉怡玲, 等. 不同六合丹外敷對急性胰腺炎大鼠血漿 TXB2、6-keto-PGF1α 的影響. 山東中醫藥大學學報, 2013, 37(3): 251-256.

1. 熊艷艷, 張劉娜, 陳本會. 外用六合丹濕敷治療急性嵌頓痔水腫的療效觀察. 華西醫學, 2016, 31(5): 955-957.
2. 李輝香, 陳本會. 中藥六合丹外敷對混合痔外剝內扎術后水腫的效果評價. 華西醫學, 2015, 30(7): 1280-1281.
3. 李黎, 邱生林, 岳曉燕. 六合丹外敷治療流行性腮腺炎患兒腮腺及睪丸腫大的療效觀察及護理. 2016, 38(1): 113-116.
4. 葉靜, 黎貴湘. 六合丹外敷治療早期哺乳期乳腺炎的療效觀察. 現代中西醫結合雜志, 2013, 22(2): 185-186.
5. 中華醫學會消化病學分會, 房靜遠, 劉文忠, 等. 中國慢性胃炎共識意見(2012 年, 上海). 中華消化雜志, 2013, 33(1): 5-16.
6. Liu D, Huang Y, Chen B, et al. Activation of mammalian target of rapamycin pathway confers adverse outcome in nonsmall cell lung carcinoma. Cancer, 2011, 117(16): 3763-3773.
7. 李芬, 李巍, 舒勤. TGF-β 與燒傷創面愈合. 創傷外科雜志, 2016, 18(12): 756-761.
8. Arend WR. The balance between IL-1 and IL-1Ra in disease. Cytokine Growth Factor Rev, 2002, 13(4/5): 323-340.
9. 石磊. 白介素 1 與幽門螺旋桿菌研究進展. 河北醫學, 2010, 16(10): 1252-1254.
10. Kapadi AS, Lee J, Torre-Amione G, et al. Tumor necrosis factor-alpha gene and protein expression in adult feline myocardium after endotoxin administration. J Clin Invest, 1995, 96(2): 1042-1052.
11. 秦建中, 秦儉, 王光. TNF-α 對表皮細胞增殖的影響. 臨床皮膚科雜志, 1995(1): 3-4.
12. Yew TL, Hung YT, Li HY, et al. Enhancement of wound healing by human multipotent stromal cell conditioned medium: the paracrine factors and p38 MAPK activation. Cell Transplant, 2011, 20(5): 693-706.
13. 吳洋, 周樹夏, 苗林. 槍彈傷口軟組織中 IL-6、IL-8、TNF-α 變化的免疫組化研究. 第二軍醫大學學報, 2001, 22(12): 1117-1118.
14. 彭小航, 黃偉, 竹林, 等. 微米六合丹外敷對急性胰腺炎大鼠氧化應激及炎癥反應水平的影響. 四川大學學報: 醫學版, 2013, 44(6): 978-981.
15. 彭小航, 趙先林, 劉怡玲, 等. 不同六合丹外敷對急性胰腺炎大鼠血漿 TXB2、6-keto-PGF1α 的影響. 山東中醫藥大學學報, 2013, 37(3): 251-256.

West China Medical Journal

The impact of Liuhedan on interleukin-1β and tumor necrosis factor-alpha during infective wound healing in rabbits

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content