Application of BenchMark automatic special staining instrument in reticular fiber staining of liver biopsy_West China Medical Journal

Authors：

CHEN Yu ¹ , YANG Mei ¹ , WANG Rong ¹ , LIAO Hongyan ² , MAO Zhigang ² ,  ZHENG Qin ²

1. Department of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;
2. Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding?author：

ZHENG Qin, Email: zhengqinhx@scu.edu.cn

Keywords：

BenchMark automatic special staining instrument; Reticular fiber staining; Foot staining; Standard operating procedure

DOI：

10.7507/1002-0179.201912082

Video：

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Objective To explore the dyeing conditions of reticular fiber staining of liver puncture tissue stained by BenchMark automatic special staining instrument, evaluate the staining effect, and share the using experience.Methods A total of 30 cases of liver puncture specimens from April to May 2019 in West China Hospital of Sichuan University were selected. They were fixed with 4% neutral formaldehyde fix solution, treated with automatic dehydrator, embedded in paraffin, stained with adhesive slides, and baked in 65℃ oven for 30 min. Each specimen was sliced in duplicate, with a thickness of 5 μm. One was reticular fiber stained with BenchMark automatic special staining instrument under the dyeing condition according to the using experience of the laboratory, and the other was reticular fiber stained by manual Foot staining method according to the standard operating procedure of the laboratory. The staining effect under microscope was observed by a chief pathologist with extensive slide-reading experience.Results The success rate of instrumental method was 100.0% (30/30), and that of the manual method was 76.7% (23/30). The difference in success rate between the two methods was statistically significant (χ²=5.143, P=0.023).Conclusion The reticular fiber staining effect of BenchMark automatic special staining instrument is stable and reliable, and the success rate is higher than that of manual Foot staining.

Citation： CHEN Yu, YANG Mei, WANG Rong, LIAO Hongyan, MAO Zhigang, ZHENG Qin. Application of BenchMark automatic special staining instrument in reticular fiber staining of liver biopsy. West China Medical Journal, 2020, 35(9): 1090-1093. doi: 10.7507/1002-0179.201912082 Copy

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1. 張立潔, 王泰齡, 劉旭華, 等. 慢性重型乙型肝炎的病理形態學表現及診斷. 中華肝臟病雜志, 2007, 15(5): 323-325.
2. 姜晶, 劉楠楠, 張繼, 等. 特殊染色在肝穿刺活檢組織中的應用價值. 北華大學學報(自然科學版), 2015, 16(4): 476-480.
3. 王曉燕, 高俊巖, 王艷霞. 網狀纖維染色在臨床病理診斷中的應用及技術. 中國醫藥導報, 2016, 13(9): 72-75.
4. 鄭海燕, 方慶全, 索文昊. 網狀纖維染色技術改進. 中國組織化學與細胞化學雜志, 2018, 27(1): 61-65.
5. 解立武, 常芳, 盧小波. 經典和改良Gomori網狀纖維染色的比較. 中國藥物與臨床, 2018, 18(1): 31-32.
6. 張紅雷, 邵晨, 王泰齡. 網狀纖維染色-渡邊改良法的應用體會. 診斷病理學雜志, 2018, 25(8): 593-594.
7. 馮倩倩, 李純, 關正元, 等. 網狀纖維染色在BenchMark特殊染色系統中的優勢. 診斷病理學雜志, 2018, 25(1): 74-75.
8. 于芳, 李純. Dakewe DP260全自動智能染色機在細胞染色中的使用體會. 診斷病理學雜志, 2017, 24(3): 205, 209.
9. 王伯沄, 李玉松, 黃高晟, 等. 病理學技術. 北京: 人民衛生出版社, 2000: 145.
10. 王德田, 董建強. 實用現代病理學技術. 北京: 中國協和醫科大學出版社, 2012: 94-95.
11. 賴美娜, 蘇學勁. 淺談網狀纖維染色失誤原因及對策. 實用醫技雜志, 2007, 14(20): 2793.
12. 曹陽, 羅虹, 羅人滋. 網狀纖維染色中脫片的解決方案. 廣西醫科大學學報, 2003, 20(6): 975.
13. 楊偉平, 吳文喬, 鄒宗楷, 等. 快速網狀纖維染色在術中快速冷凍切片病理診斷中的應用. 臨床與實驗病理學雜志, 2016, 32(10): 1181-1182.
14. 王玉芳, 徐曉艷, 武彥. 常用特殊染色在病理診斷中的應用. 實用醫技雜志, 2013, 20(12): 1357-1358.
15. 中華醫學會. 臨床技術操作規范病理學分冊. 北京: 人民軍醫出版社, 2004: 37-38.
16. 王炳海, 方慶全, 葉美華. 提高網狀纖維染色病理實驗教學質量的探討. 齊齊哈爾醫學院學報, 2013, 34(7): 1029-1030.

West China Medical Journal

Application of BenchMark automatic special staining instrument in reticular fiber staining of liver biopsy

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