MORPHOLOGY AND PATHOLOGICAL CHANGES OF POLYTETRAFLUOROETHYLENE ARTIFICIAL TRACHEAL TRANSPLANTATION_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

LIGang ¹ , ZHANGJing ¹ , CUIXiaohai ¹ , ZHAOYang ⁴ , CAOPeilong ² , LULu ³ ,  LIXiaojun ¹

1. The 2nd Department of Thoracic Surgery the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an Shaanxi, 710061, P. R. China;
2. Department of Pathology, Xi'an Shaanxi the First Affiliated Hospital of Xi'an Jiaotong University, 710061, P. R. China;
3. Department of Operating Room, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an Shaanxi, 710061, P. R. China;
4. Department of Tumor Surgery, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an Shaanxi, 710061, P. R. China;

Corresponding?author：

LIXiaojun, Email: dooopenit@163.com

Keywords：

Artificial tracheal prosthesis; Tracheal reconstruction; Polytetrafluoroethylene; EpithelizationRabbit

DOI：

10.7507/1002-1892.20140194

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To observe the morphological and pathological changes after transplantation of polytetrafluoroethylene (PTFE) in vivo. Methods PTFE microporous polypropylene tube which was encircled by spiral steel wire was used to prepare the artificial trachea.Forty New Zealand white rabbits (weighing,4-5 kg) were selected,and were divided into 2 groups.After the cervical trachea (2 cm in length) was removed,the end-to-end anastomosis between the trachea and PTFE artificial trachea was performed in the experimental group (n=20),and end-to-end anastomosis of the trachea in the control group (n=20).The survival of the rabbits was observed after operation;the X-ray,gross,and histological observations were carried out at 2,4,and 6 months after operation.The longitudinal tensile and radial support biomechanical tests were performed before and after transplantation. Results The survival time was more than 2 months and the artificial airway was patency in 15 rabbits of the experimental group;the tissue outside the artificial trachea was like tracheal tissue,which filled in the defect,but it was more than 4 months.X-ray observation showed that the PTFE artificial trachea had no obvious displacement in the experimental group,and no tracheostenosis was observed in the control group.After 2 months,there was no epithelial tissue on the artificial airway wall;after 4 months,there was some epithelial cells on the artificial airway wall,incomplete endothelialization and trachea layer structure were seen with no tracheal ciliated columnar epithelium;after 6 months,the artificial trachea wall was covered with epithelium basically,and some ciliated columnar epithelium cells were found,which had the physiological function of the trachea.The transplanted PTFE artificial trachea could keep the stability of the biological mechanics performance,and could be used for the rabbit tracheal reconstruction. Conclusion PTFE artificial trachea can induce to form a tracheal tissue in the trachea tissues of recipients,each layer of the trachea is relatively complete and the experiment animals can be short-term survival.

Citation： LIGang, ZHANGJing, CUIXiaohai, ZHAOYang, CAOPeilong, LULu, LIXiaojun. MORPHOLOGY AND PATHOLOGICAL CHANGES OF POLYTETRAFLUOROETHYLENE ARTIFICIAL TRACHEAL TRANSPLANTATION. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(7): 879-884. doi: 10.7507/1002-1892.20140194 Copy

1.	Delaere PR.Tracheal transplantation.Curr Opin Pulm Med,2012,18(4):313-320.
2.	Fishman JM,De Coppi P,Elliott MJ,et al.Airway tissue engineering.Expert Opin Biol Ther,2011,11(12):1623-1635.
3.	王嵩,史宏燦.人工氣管研究進展.中國修復重建外科雜志,2011,25(3):365-368.
4.	姚佳,李曉軍,崔曉海,等.兔人工氣管移植后成活時間與C-反應蛋白的相關性.中國組織工程研究,2014,18(10):718-723.
5.	Osada H.Artificial trachea.J Bronchol,2006,13(1):39-43.
6.	劉鑫,姜召陽,秦永.碳-碳復合材料氣管重建的實驗研究.中華耳鼻咽喉頭頸外科雜志,2010,45(8):669-673.
7.	Jungebluth P,Bader A,Baiguera S,et al.The concept of in vivo airway tissue engineering.Biomaterials,2012,33(17):4319-4326.
8.	Du XF,Kwon SK,Song JJ,et al.Tracheal reconstruction by mesenchymal stem cells with small intestine submucosa in rabbits.Int J Pediatr Otorhinolaryngol,2012,76(3):345-351.
9.	Haag J,Baiguera S,Jungebluth P,et al.Biomechanical and angiogenic properties of tissue-engineered rat trachea using genipin cross-linked decellularized tissue.Biomaterials,2012,33(3):780-789.
10.	Baiguera S,Del Gaudio C,Jaus MO,et al.Long-term changes to in vitro preserved bioengineered human trachea and their implications for decellularized tissues.Biomaterials,2012,33(14):3662-3672.
11.	Zhao F,Zhang Y,Liu S.Artificial trachea reconstruction with two-stage approach using memory-alloy mesh.Chin Med J (Engl),2003,116(12):1949-1951.
12.	袁海根,曾金芳,楊杰.聚四氟乙烯改性研究進展.塑料工業,2005,3(8):7-10.
13.	史宏燦,徐志飛,趙學維,等.人工氣管重建氣管的實驗研究.中華胸心血管外科雜志,2004,20(2):70-72.
14.	林海平,曹子昂,顧旭東,等.膠原涂層記憶合金人工氣管的構建.上海交通大學學報:醫學版,2007,27(11):1331-1334.
15.	Neville WE,Bolanowski JP,Kotia GG.Clinical experience with the silicone tracheal prothesis.J Thorac Cardiovasc Surg,1990,99(4):604-613.
16.	Kanemaru S,Hirano S,Umeda H,et al.A tissue-engineering approach for stenosis of the trachea and/or cricoid.Acta Otolaryngol Suppl,2010,(563):79-83.
17.	陳萬生,焦英華,郝雁冰,等.人工氣管移植的病理學變化.中華胸心血管外科雜志,2013,29(2):99-101.
18.	趙鳳瑞,張銀合,劉德若,等.記憶合金網二期成形人工氣管置換術.中華外科雜志,2003,4(3):201-204.
19.	寇秀娟,陳曉紅.長段氣管缺損后重建術的研究進展.國際耳鼻喉頭頸外科雜志,2012,36(5):266-269.
20.	陳堅偉,張迪.3D打印技術醫學應用綜述與展望.電腦知識與技術,2013,19(15):3632-3633.

1. Delaere PR.Tracheal transplantation.Curr Opin Pulm Med,2012,18(4):313-320.
2. Fishman JM,De Coppi P,Elliott MJ,et al.Airway tissue engineering.Expert Opin Biol Ther,2011,11(12):1623-1635.
3. 王嵩,史宏燦.人工氣管研究進展.中國修復重建外科雜志,2011,25(3):365-368.
4. 姚佳,李曉軍,崔曉海,等.兔人工氣管移植后成活時間與C-反應蛋白的相關性.中國組織工程研究,2014,18(10):718-723.
5. Osada H.Artificial trachea.J Bronchol,2006,13(1):39-43.
6. 劉鑫,姜召陽,秦永.碳-碳復合材料氣管重建的實驗研究.中華耳鼻咽喉頭頸外科雜志,2010,45(8):669-673.
7. Jungebluth P,Bader A,Baiguera S,et al.The concept of in vivo airway tissue engineering.Biomaterials,2012,33(17):4319-4326.
8. Du XF,Kwon SK,Song JJ,et al.Tracheal reconstruction by mesenchymal stem cells with small intestine submucosa in rabbits.Int J Pediatr Otorhinolaryngol,2012,76(3):345-351.
9. Haag J,Baiguera S,Jungebluth P,et al.Biomechanical and angiogenic properties of tissue-engineered rat trachea using genipin cross-linked decellularized tissue.Biomaterials,2012,33(3):780-789.
10. Baiguera S,Del Gaudio C,Jaus MO,et al.Long-term changes to in vitro preserved bioengineered human trachea and their implications for decellularized tissues.Biomaterials,2012,33(14):3662-3672.
11. Zhao F,Zhang Y,Liu S.Artificial trachea reconstruction with two-stage approach using memory-alloy mesh.Chin Med J (Engl),2003,116(12):1949-1951.
12. 袁海根,曾金芳,楊杰.聚四氟乙烯改性研究進展.塑料工業,2005,3(8):7-10.
13. 史宏燦,徐志飛,趙學維,等.人工氣管重建氣管的實驗研究.中華胸心血管外科雜志,2004,20(2):70-72.
14. 林海平,曹子昂,顧旭東,等.膠原涂層記憶合金人工氣管的構建.上海交通大學學報:醫學版,2007,27(11):1331-1334.
15. Neville WE,Bolanowski JP,Kotia GG.Clinical experience with the silicone tracheal prothesis.J Thorac Cardiovasc Surg,1990,99(4):604-613.
16. Kanemaru S,Hirano S,Umeda H,et al.A tissue-engineering approach for stenosis of the trachea and/or cricoid.Acta Otolaryngol Suppl,2010,(563):79-83.
17. 陳萬生,焦英華,郝雁冰,等.人工氣管移植的病理學變化.中華胸心血管外科雜志,2013,29(2):99-101.
18. 趙鳳瑞,張銀合,劉德若,等.記憶合金網二期成形人工氣管置換術.中華外科雜志,2003,4(3):201-204.
19. 寇秀娟,陳曉紅.長段氣管缺損后重建術的研究進展.國際耳鼻喉頭頸外科雜志,2012,36(5):266-269.
20. 陳堅偉,張迪.3D打印技術醫學應用綜述與展望.電腦知識與技術,2013,19(15):3632-3633.

Chinese Journal of Reparative and Reconstructive Surgery

MORPHOLOGY AND PATHOLOGICAL CHANGES OF POLYTETRAFLUOROETHYLENE ARTIFICIAL TRACHEAL TRANSPLANTATION

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content