ESTABLISHMENT OF A NEW RADIUS DEFECT MODEL BASED ON ULNA ANATOMICAL MEASUREMENT IN RABBITS_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

LIUHanjiang , GUOYing ,  MEIWei

Zhengzhou Orthopaedics Hospital, Zhengzhou Henan, 450052, P. R. China;

Corresponding?author：

MEIWei, Email: meiwei9606@163.com

Keywords：

Radius defect; Animal model; Micro-CT; Anatomical measurement; Rabbit

DOI：

10.7507/1002-1892.20160036

Video：

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Objective To introduce a new bone defect model based on the anatomical measurement of radius and ulna in rabbits for offering a standard model for further tissue engineering research. Methods Fifteen healthy 4-month-old New Zealand rabbits were selected for anatomic measurement and radiological measurement of the radius and ulna. Another 30 healthy 4-month-old New Zealand rabbits were randomly divided into groups A, B, and C (n=10). The radius bone defect was created bilaterally in 3 groups. In group A, the periosteum and interosseous membranes were fully removed with jig-saw by approach between extensor carpi radialis muscle and musculus extensor digitorum. The periosteum and interosseous membranes were fully removed in group B, and only periosteum was removed in group C with electric-saw by approach between extensor carpi radialis muscle and flexor digitorum profundus based on anatomical analysis results of ulnar and radial measurement. The gross observation, X-ray, micro-CT three-dimensional reconstruction, bone mineral density (BMD), and bone mineral content (BMC) were observed and recorded at immediate and 15 weeks after operation. HE staining and Masson staining were performed to observe bone formation in the defect areas. Results Blood vessel injury (1 rabbit), tendon injury (2 rabbits), postoperative hematoma (1 rabbit), and infection (1 rabbit) occurred in group A, postoperative infection (1 rabbit) in group C, and no postoperative complications in group B; the complication rate of group A (50%) was significantly higher than that of groups B (0%) and C (10%) (P<0.05). The radiological examination showed bone defects were fully repaired in groups A and B at 15 weeks, but bridging callus formation was observed in group C. There was no significant difference in BMC and BMD among 3 groups (P>0.05). HE staining and Masson staining results showed bone formation in group A, with structure disturbance and sclerosis. New bone formed in groups B and C, cartilage cells were observed in the center of bone cells. Conclusion The radius bone defect model established by approach between extensor carpi radialis muscle and flexor digitorum profundus is an ideal model because of better exposures, less intra-operative blood loss, less complications. Interosseous membranes play a role in bone tissue repair process, and the mechanism needs further study.

Citation： LIUHanjiang, GUOYing, MEIWei. ESTABLISHMENT OF A NEW RADIUS DEFECT MODEL BASED ON ULNA ANATOMICAL MEASUREMENT IN RABBITS. Chinese Journal of Reparative and Reconstructive Surgery, 2016, 30(2): 173-177. doi: 10.7507/1002-1892.20160036 Copy

1.	?Johnson EE, Urist MR, Schmalzried TP, et al. Autogeneic cancellous bone grafts in extensive segmental ulnar defects in dogs. Effects of xenogeneic bovine bone morphogenetic protein without and with interposition of soft tissues and interruption of blood supply. Clin Orthop Relat Res, 1989, (243):254-265.
2.	Roohani-Esfahani SI, Dunstan CR, Davies B, et al. Repairing a critical-sized bone defect with highly porous modified and unmodified baghdadite scaffolds. Acta Biomater, 2012, 8(11):4162-4172.
3.	吳東進, 郝愛華, 張程, 等. 內皮祖細胞與納米羥基磷灰石/膠原/聚乳酸復合物在修復兔骨缺損中的作用. 中華醫學雜志, 2012, 92(23):1630-1634.
4.	王永剛, 裴國獻, 張洪濤, 等. 兔股骨干缺損模型的制備及在組織工程骨實驗中的應用. 中華創傷骨科雜志, 2005, 7(10):971-974.
5.	Shafiei-Sarvestani Z, Oryan A, Bigham AS, et al. The effect of hydroxyapatite-hPRP, and coral-hPRP on bone healing in rabbits:radiological, biomechanical, macroscopic and histopathologic evaluation. Int J Surg, 2012, 10(2):96-101.
6.	宋坤修, 何愛詠, 謝求恩. 納米羥基磷灰石/膠原復合材料結合血管內皮生長因子修復兔橈骨缺損. 中國組織工程研究與臨床康復, 2009, 13(34):6624-6628.
7.	蘭小勇, 周初松, 田京, 等. 海藻酸鈣/納米羥基磷灰石/膠原復合材料修復兔橈骨缺損. 中國醫學科學院學報, 2009, 31(4):459-463.
8.	楊潤功, 裴國獻, 徐達傳. 四種BMP載體修復骨缺損的形態學觀察. 中國臨床解剖學雜志, 2001, 19(4):350-352.
9.	林博文, 徐忠世, 肖德明, 等. 實驗性骨不連模型的制作. 中山醫科大學學報, 2002, 23(5):331-335.
10.	蘭小勇. 納米羥基磷灰石海藻酸鈣膠原復合物骨修復材料的生物相容性及動物實驗研究. 廣州:南方醫科大學, 2009.
11.	趙明東, 潘志宏, 朱梁豫, 等. 建立橈骨缺損模型:骨缺損標準尺寸的確立. 中國組織工程研究與臨床康復, 2011, 15(2):213-218.
12.	王玉學, 李鄭民, 畢鄭鋼. 建立兔橈骨缺損動物模型的實驗研究. 中國地方病防治雜志, 2008, 23(1):60-61.
13.	Yang F, Wang J, Hou J, et al. Bone regeneration using cell-mediated responsive degradable PEG-based scaffolds incorporating with rhBMP-2. Biomaterials, 2013, 34(5):1514-1528.
14.	Kasten P, Vogel J, Geiger F, et al. The effect of platelet-rich plasma on healing in critical-size long-bone defects. Biomaterials, 2008, 29(29):3983-3992.
15.	Niemeyer P, Szalay K, Luginbuhl R, et al. Transplantation of human mesenchymal stem cells in a non-autogenous setting for bone regeneration in a rabbit critical-size defect model. Acta Biomater, 2010, 6(3):900-908.
16.	Geiger F, Lorenz H, Xu W, et al. VEGF producing bone marrow stromal cells (BMSC) enhance vascularization and resorption of a natural coral bone substitute. Bone, 2007, 41(4):516-522.

1. ?Johnson EE, Urist MR, Schmalzried TP, et al. Autogeneic cancellous bone grafts in extensive segmental ulnar defects in dogs. Effects of xenogeneic bovine bone morphogenetic protein without and with interposition of soft tissues and interruption of blood supply. Clin Orthop Relat Res, 1989, (243):254-265.
2. Roohani-Esfahani SI, Dunstan CR, Davies B, et al. Repairing a critical-sized bone defect with highly porous modified and unmodified baghdadite scaffolds. Acta Biomater, 2012, 8(11):4162-4172.
3. 吳東進, 郝愛華, 張程, 等. 內皮祖細胞與納米羥基磷灰石/膠原/聚乳酸復合物在修復兔骨缺損中的作用. 中華醫學雜志, 2012, 92(23):1630-1634.
4. 王永剛, 裴國獻, 張洪濤, 等. 兔股骨干缺損模型的制備及在組織工程骨實驗中的應用. 中華創傷骨科雜志, 2005, 7(10):971-974.
5. Shafiei-Sarvestani Z, Oryan A, Bigham AS, et al. The effect of hydroxyapatite-hPRP, and coral-hPRP on bone healing in rabbits:radiological, biomechanical, macroscopic and histopathologic evaluation. Int J Surg, 2012, 10(2):96-101.
6. 宋坤修, 何愛詠, 謝求恩. 納米羥基磷灰石/膠原復合材料結合血管內皮生長因子修復兔橈骨缺損. 中國組織工程研究與臨床康復, 2009, 13(34):6624-6628.
7. 蘭小勇, 周初松, 田京, 等. 海藻酸鈣/納米羥基磷灰石/膠原復合材料修復兔橈骨缺損. 中國醫學科學院學報, 2009, 31(4):459-463.
8. 楊潤功, 裴國獻, 徐達傳. 四種BMP載體修復骨缺損的形態學觀察. 中國臨床解剖學雜志, 2001, 19(4):350-352.
9. 林博文, 徐忠世, 肖德明, 等. 實驗性骨不連模型的制作. 中山醫科大學學報, 2002, 23(5):331-335.
10. 蘭小勇. 納米羥基磷灰石海藻酸鈣膠原復合物骨修復材料的生物相容性及動物實驗研究. 廣州:南方醫科大學, 2009.
11. 趙明東, 潘志宏, 朱梁豫, 等. 建立橈骨缺損模型:骨缺損標準尺寸的確立. 中國組織工程研究與臨床康復, 2011, 15(2):213-218.
12. 王玉學, 李鄭民, 畢鄭鋼. 建立兔橈骨缺損動物模型的實驗研究. 中國地方病防治雜志, 2008, 23(1):60-61.
13. Yang F, Wang J, Hou J, et al. Bone regeneration using cell-mediated responsive degradable PEG-based scaffolds incorporating with rhBMP-2. Biomaterials, 2013, 34(5):1514-1528.
14. Kasten P, Vogel J, Geiger F, et al. The effect of platelet-rich plasma on healing in critical-size long-bone defects. Biomaterials, 2008, 29(29):3983-3992.
15. Niemeyer P, Szalay K, Luginbuhl R, et al. Transplantation of human mesenchymal stem cells in a non-autogenous setting for bone regeneration in a rabbit critical-size defect model. Acta Biomater, 2010, 6(3):900-908.
16. Geiger F, Lorenz H, Xu W, et al. VEGF producing bone marrow stromal cells (BMSC) enhance vascularization and resorption of a natural coral bone substitute. Bone, 2007, 41(4):516-522.

Chinese Journal of Reparative and Reconstructive Surgery

ESTABLISHMENT OF A NEW RADIUS DEFECT MODEL BASED ON ULNA ANATOMICAL MEASUREMENT IN RABBITS

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