Cervical heart transplantation in mice model_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

CAO Jianbin ,  GONG Yongquan , WEI Chengxin , LI Fuli , LUO Peng

Department of Cardiothoracic Surgery, Liuzhou General Hospital, Liuzhou, 545003, Guangxi, P.R.China;

Corresponding?author：

GONG Yongquan, Email: gongyongquan01@hotmail.com

Keywords：

Heart transplantation; animal model; mice

DOI：

10.7507/1007-4848.201609010

Video：

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Objective To investigate how to establish stable mice cervical heart transplantation model. Methods Totally, 40 male C57 mice with the age of 6-8 weeks and weight of 19-24 g were randomly divided into recipients and donors (n=20 in each group). Mice cervical heart transplantation model was established by connecting the ascending aorta of donors to the right cervical common artery of recipients through end to side anastmosis and the pulmonary artery of donors to the right external jugular vein of recipients through end to end anastmosis. Results More than 95% recipients survived after surgery. Cold ischemia time was 15±5 min, warm ischemia time 23±6 min, and the whole operation took about 55±15 min. The recipients survived more than 30 d with functional heart grafts. Histologically, there was no difference between the heart graft one month after the transplantion and the normal heart. Conclusion Cervical heart transplantation of mice model is reliable and feasible, which is easy to monitor the survival condition of heart graft by visual examination and palpation, which will benefit the basic research in transplantation field.

Citation： CAO Jianbin, GONG Yongquan, WEI Chengxin, LI Fuli, LUO Peng. Cervical heart transplantation in mice model. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2017, 24(11): 886-889. doi: 10.7507/1007-4848.201609010 Copy

1.	Corry RJ, Winn HJ, Russell PS. Primarily vascularized allografts of hearts in mice. The role of H-2D, H-2K, and non-H-2 antigens in rejection. Transplantation, 1973, 16(4):343-350.
2.	Plenter RJ, Zamora MR, Grazia TJ. Four decades of vascularized heterotopic cardiac transplantation in the mouse. J Invest Surg, 2013, 26(4):223-228.
3.	何瑩, 宮念樵. 心臟移植動物模型的構建. 實用器官移植電子雜志, 2016, 4(2):88-92.
4.	郁雷, 高麗. 小鼠腹部心臟移植模型建立及并發癥預防. 中國胸心血管外科臨床雜志, 2014, 21(2):249-252.
5.	單忠貴, 馬春野, 廖崇先, 等. 小鼠腹腔異位心臟移植模型制作技術的改進. 吉林大學學報(醫學版), 2009, 35(1): 188-190.
6.	陳心足, Wang SJ, Zhou C, 等. 同種異體小鼠腹腔異位心臟移植模型的學習曲線. 中國胸心血管外科臨床雜志, 2015, 22(9): 879-882.
7.	陳義發, 尹輝, 梁賓勇, 等. 兩種異位心臟移植小鼠模型的建立和比較. 中國實驗動物學報, 2010, 18(1):17-20.
8.	周景師, 竇科峰, 孫凱, 等. 兩種小鼠異位心臟移植模型的復制及比較. 醫學研究生學報, 2003, 16(9):653-655.
9.	Chen ZH. A technique of cervical heterotopic heart transplantation in mice. Transplantation, 1991, 52(6): 1099-1101.
10.	Gong W, Thornley T, Whitcher GH, et al. Introduction of modified cervical cardiac transplant model in mice. Exp Clin Transplant, 2012, 10(2):158-162.
11.	Fang J, He L, Wang SQ, et al. A simplified two-stitch sleeve technique for arterial anastomosis of cervical heterotopic cardiac transplantation in mice. Am J Transl Res, 2013, 5(5): 521-529.
12.	陳文偉, 楊亦榮, 夏鵬, 等. 小鼠頸部異位心臟移植手術的改良. 溫州醫學院學報, 2011, 41(3): 252-254.
13.	柳祎, 孫宗全. 小鼠頸部異位心臟移植技術的改進. 華中科技大學學報(醫學版), 2003, 32(5): 522-525.
14.	Ratschiller T, Deutsch MA, Calzada-Wack J, et al. Heterotopic cervical heart transplantation in mice. J Vis Exp, 2015, (102): e52907.
15.	Oberhuber R, Cardini B, Kofler M. Murine cervical heart transplantation model using a modified cuff technique. J Vis Exp, 2014; (92): e50753.
16.	張向輝, 劉曉萍, 宋麗紳. 介紹一種改良的小鼠頸部心臟移植模型建立方法. 山東醫藥, 2014, 54(21): 29-32.
17.	劉琛, 王斌, 王春生. 單人袖套法建立小鼠頸部心臟移植模型. 上海醫學, 2013, 36(5): 446-448.
18.	Matsuura A, Abe T, Yasuura K. Simplified mouse cervical heart transplantation using a cuff technique. Transplantation, 1991, 51(4): 896-898.
19.	高開柱, 孫宗全, 杜心靈, 等. 套管法建立小鼠頸部心臟移植模型的探討. 華中科技大學學報(醫學版), 2006, 35(5): 638-640.
20.	Niimi M. The technique for heterotopic cardiac transplantationin mice: experience of 3000 operations by one surgeon. J Heart Lung Transplant, 2001, 20(10): 1123-1128.

1. Corry RJ, Winn HJ, Russell PS. Primarily vascularized allografts of hearts in mice. The role of H-2D, H-2K, and non-H-2 antigens in rejection. Transplantation, 1973, 16(4):343-350.
2. Plenter RJ, Zamora MR, Grazia TJ. Four decades of vascularized heterotopic cardiac transplantation in the mouse. J Invest Surg, 2013, 26(4):223-228.
3. 何瑩, 宮念樵. 心臟移植動物模型的構建. 實用器官移植電子雜志, 2016, 4(2):88-92.
4. 郁雷, 高麗. 小鼠腹部心臟移植模型建立及并發癥預防. 中國胸心血管外科臨床雜志, 2014, 21(2):249-252.
5. 單忠貴, 馬春野, 廖崇先, 等. 小鼠腹腔異位心臟移植模型制作技術的改進. 吉林大學學報(醫學版), 2009, 35(1): 188-190.
6. 陳心足, Wang SJ, Zhou C, 等. 同種異體小鼠腹腔異位心臟移植模型的學習曲線. 中國胸心血管外科臨床雜志, 2015, 22(9): 879-882.
7. 陳義發, 尹輝, 梁賓勇, 等. 兩種異位心臟移植小鼠模型的建立和比較. 中國實驗動物學報, 2010, 18(1):17-20.
8. 周景師, 竇科峰, 孫凱, 等. 兩種小鼠異位心臟移植模型的復制及比較. 醫學研究生學報, 2003, 16(9):653-655.
9. Chen ZH. A technique of cervical heterotopic heart transplantation in mice. Transplantation, 1991, 52(6): 1099-1101.
10. Gong W, Thornley T, Whitcher GH, et al. Introduction of modified cervical cardiac transplant model in mice. Exp Clin Transplant, 2012, 10(2):158-162.
11. Fang J, He L, Wang SQ, et al. A simplified two-stitch sleeve technique for arterial anastomosis of cervical heterotopic cardiac transplantation in mice. Am J Transl Res, 2013, 5(5): 521-529.
12. 陳文偉, 楊亦榮, 夏鵬, 等. 小鼠頸部異位心臟移植手術的改良. 溫州醫學院學報, 2011, 41(3): 252-254.
13. 柳祎, 孫宗全. 小鼠頸部異位心臟移植技術的改進. 華中科技大學學報(醫學版), 2003, 32(5): 522-525.
14. Ratschiller T, Deutsch MA, Calzada-Wack J, et al. Heterotopic cervical heart transplantation in mice. J Vis Exp, 2015, (102): e52907.
15. Oberhuber R, Cardini B, Kofler M. Murine cervical heart transplantation model using a modified cuff technique. J Vis Exp, 2014; (92): e50753.
16. 張向輝, 劉曉萍, 宋麗紳. 介紹一種改良的小鼠頸部心臟移植模型建立方法. 山東醫藥, 2014, 54(21): 29-32.
17. 劉琛, 王斌, 王春生. 單人袖套法建立小鼠頸部心臟移植模型. 上海醫學, 2013, 36(5): 446-448.
18. Matsuura A, Abe T, Yasuura K. Simplified mouse cervical heart transplantation using a cuff technique. Transplantation, 1991, 51(4): 896-898.
19. 高開柱, 孫宗全, 杜心靈, 等. 套管法建立小鼠頸部心臟移植模型的探討. 華中科技大學學報(醫學版), 2006, 35(5): 638-640.
20. Niimi M. The technique for heterotopic cardiac transplantationin mice: experience of 3000 operations by one surgeon. J Heart Lung Transplant, 2001, 20(10): 1123-1128.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Cervical heart transplantation in mice model

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