A model of transplanting neonatal cardiomycytes into the wall of rat inferior vena cava_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

ZHOU Xingang ¹ , NI Lixin ² , CHI Cao ² ,  LIU Hongyu ² , YAO Ye ²

1. Department of Cardiovascular Medicine, Harbin Medical University, Harbin, 150001, P.R.China;
2. Department of Cardiovascular Surgery, Harbin Medical University, Harbin, 150001, P.R.China;

Corresponding?author：

LIU Hongyu, Email: hyliu1963@163.com

Keywords：

Neonatal rat; cardiomyocyte; cell transplantation; the wall of inferior vena cava; spontaneously beating

DOI：

10.7507/1007-4848.201612050

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To establish a model of transplanting neonatal cardiomycytes into the wall of rat inferior vena cava. Methods Neonatal cardiomyocytes (n=6, 5×10⁶cells each, A group) or medium (n=6, B group) only were transplanted into the wall of inferior vena cava in female Fisher rats. At 21 days after transplantation, the contraction of transplanted cardiomyocytes was assessed and the inferior vena cava was processed for histology. Results Distinct rhythmic beating of the vena cava at the site of cell transplantation before and after the aorties were clamped (at a rate 141± 47 rpm and 88± 44 rpm which was dramaticly lower than aortic beating, with a statistical difference at P value of 0.03). Cardiomyocyte was seen in 6 rats who had neonatal cardiomyocyte transplantation, but not in 6 rats receiving media. Hematoxylin and eosin staining showed viable cardiomyocytes in the wall of the vena cava in 6 rats treated with neonatal cardiomyocytes, but not in 6 rats receiving media. Conclusion This study shows that neonatal cardiomyocytes can survive, mature and spontaneously and rhythmically contract after they are transplanted in the wall of inferior vena cava.

Citation： ZHOU Xingang, NI Lixin, CHI Cao, LIU Hongyu, YAO Ye. A model of transplanting neonatal cardiomycytes into the wall of rat inferior vena cava. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2018, 25(3): 233-236. doi: 10.7507/1007-4848.201612050 Copy

1.	鐘婷婷, 侯婧瑛, 郭天柱, 等. 心肌干細胞移植可短期內改善心肌梗死心電生理穩定性和提高室顫閾值. 中國組織工程研究, 2015, 28(19): 4537-4543.
2.	張漢平, 蔡曉慶, 馬凌. 脂肪間充質干細胞移植治療兔急性心肌梗死的實驗. 國際心血管病雜志, 2015, 6(42): 407-411.
3.	王巍, 李肖甫, 李中健. 不同孕周人臍帶間充質干細胞移植改善心肌梗死模型心臟功能的比較. 中國組織工程研究, 2016, 20(6): 799-806.
4.	陳曉明, 崔丹陽, 張明. 急性心肌梗死合并心衰患者應用干細胞移植的臨床觀察. 大連醫科大學學報, 2014, 36(2): 157-159.
5.	侯江龍, 武忠. 細胞移植治療心肌梗死的研究進展. 中國胸心血管外科臨床雜志, 2015, 22(5): 486-490.
6.	Wendel JS, Ye L, Tao R, et al. Functional effects of a tissue-engineered cardiac patch from human induced pluripotent stem cell-derived cardiomyocytes in a rat infarct model. Stem Cells Transl Med, 2015, 4(11): 1324-1332.
7.	Kloner RA, Dai W, Shi J, et al. Rebuilding the infarcted heart with noncellular material. Regen Med, 2015, 10(6): 683-685.
8.	Khan M, Nickoloff E, Abramova T, et al. Embryonic stem cell-derived exosomes promote endogenous repair mechanisms and enhance cardiac function following myocardial infarction. Circ Res, 2015, 117(1): 52-64.
9.	Gulati AK. Regeneration pattern of cardiac and skeletal muscle after transplantation into a skeletal muscle bed in rats. Anat Rec, 1995, 242(2): 188-194.
10.	Li RK, Mickle DA, Weisel RD, et al. In vivo survival and function of transplanted rat cardiomyocytes.Circ Res, 1996, 78(2): 283-288.
11.	Dai W, Hale SL, Kloner RA. Implantation of immature neonatal cardiac cells into the wall of the aorta in rats a novel model for studying morphological and functional development of heart cells in an extracardiac environment. Circulation, 2004, 110(3): 324-329.
12.	Dai W, Hale SL, Kloner RA. Development of a spontaneously beating vein by cardiomyocyte transplantation in the wall of the inferior vena cava in a rat: A pilot study.J Vasc Surg, 2007, 45(4): 817-820.
13.	Dai W, Hale SL, Kloner RA. Cardiac cells implanted within the outer aortic wall of rats generate measurable contractile force.Regen Med, 2006, 1(1): 119-124.
14.	Biermann D, Eder A, Arndt F, et al.Towards a tissue-engineered contractile Fontan-conduit: the fate of cardiac myocytes in the subpulmonary circulation. PLoS One, 2016, 11(11): e0166963.

1. 鐘婷婷, 侯婧瑛, 郭天柱, 等. 心肌干細胞移植可短期內改善心肌梗死心電生理穩定性和提高室顫閾值. 中國組織工程研究, 2015, 28(19): 4537-4543.
2. 張漢平, 蔡曉慶, 馬凌. 脂肪間充質干細胞移植治療兔急性心肌梗死的實驗. 國際心血管病雜志, 2015, 6(42): 407-411.
3. 王巍, 李肖甫, 李中健. 不同孕周人臍帶間充質干細胞移植改善心肌梗死模型心臟功能的比較. 中國組織工程研究, 2016, 20(6): 799-806.
4. 陳曉明, 崔丹陽, 張明. 急性心肌梗死合并心衰患者應用干細胞移植的臨床觀察. 大連醫科大學學報, 2014, 36(2): 157-159.
5. 侯江龍, 武忠. 細胞移植治療心肌梗死的研究進展. 中國胸心血管外科臨床雜志, 2015, 22(5): 486-490.
6. Wendel JS, Ye L, Tao R, et al. Functional effects of a tissue-engineered cardiac patch from human induced pluripotent stem cell-derived cardiomyocytes in a rat infarct model. Stem Cells Transl Med, 2015, 4(11): 1324-1332.
7. Kloner RA, Dai W, Shi J, et al. Rebuilding the infarcted heart with noncellular material. Regen Med, 2015, 10(6): 683-685.
8. Khan M, Nickoloff E, Abramova T, et al. Embryonic stem cell-derived exosomes promote endogenous repair mechanisms and enhance cardiac function following myocardial infarction. Circ Res, 2015, 117(1): 52-64.
9. Gulati AK. Regeneration pattern of cardiac and skeletal muscle after transplantation into a skeletal muscle bed in rats. Anat Rec, 1995, 242(2): 188-194.
10. Li RK, Mickle DA, Weisel RD, et al. In vivo survival and function of transplanted rat cardiomyocytes.Circ Res, 1996, 78(2): 283-288.
11. Dai W, Hale SL, Kloner RA. Implantation of immature neonatal cardiac cells into the wall of the aorta in rats a novel model for studying morphological and functional development of heart cells in an extracardiac environment. Circulation, 2004, 110(3): 324-329.
12. Dai W, Hale SL, Kloner RA. Development of a spontaneously beating vein by cardiomyocyte transplantation in the wall of the inferior vena cava in a rat: A pilot study.J Vasc Surg, 2007, 45(4): 817-820.
13. Dai W, Hale SL, Kloner RA. Cardiac cells implanted within the outer aortic wall of rats generate measurable contractile force.Regen Med, 2006, 1(1): 119-124.
14. Biermann D, Eder A, Arndt F, et al.Towards a tissue-engineered contractile Fontan-conduit: the fate of cardiac myocytes in the subpulmonary circulation. PLoS One, 2016, 11(11): e0166963.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

A model of transplanting neonatal cardiomycytes into the wall of rat inferior vena cava

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