The relationship between miR-3187-5p and postoperative atrial fibrillation after coronary artery bypass grafting: A prospective cohort study_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

YAN Ziyi ¹ , ZHAO Haibo ² , HE Chunyue ² ,  LIU Yisi ¹

1. School of Nursing, Capital Medical University, Beijing, 100069, P. R. China;
2. Heart Center, Chao-Yang Hospital, Capital Medical University, Beijing, 100020, P. R. China;

Corresponding?author：

LIU Yisi, Email: asceticsisi@aliyun.com

Keywords：

Postoperative atrial fibrillation; miR-3187-5p; coronary artery bypass grafting; bioinformatics; prospective cohort study

DOI：

10.7507/1007-4848.202206018

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the relationship between miR-3187-5p in peripheral blood and pericardial drainage after coronary artery bypass grafting (CABG) and postoperative atrial fibrillation (POAF). Methods Patients who underwent CABG in the Heart Center of Beijing Chao-Yang Hospital from March to May 2022 were enrolled. Peripheral blood and pericardial drainage were collected at 0 h after surgery (immediate time for patients to return to ICU from operating room) to detect miR-3187-5p, and perioperative confounding factors were also collected. The miR-3187-5p was measured by quantitative real-time PCR and its regulated target genes were analyzed by bioinformatics. Results A total of 15 patients were enrolled, including 9 males and 6 females with an average age of 65.6±8.2 years. The incidence rate of POAF was 40.0%. miR-3187-5p in pericardial drainage at 0 h after surgery was an independent predictor for POAF. A total of 1 642 target genes of miR-3187-5p were predicted. GO function enrichment analysis and KEGG signal pathway enrichment analysis showed that target genes of miR-3187-5p were enriched in TGF-β, MAPK, Wnt and other classical collagen metabolic signal pathways, which might activate collagen metabolism by negatively regulating SMAD6 and other inhibitors of the pathways. Conclusion This study is the first to find that miR-3187-5p in pericardial drainage at 0 h after surgery is a potential, novel, and predictive factor for POAF, which may be related to the regulation of myocardial fibrosis signal pathways like TGF-β, MAPK and Wnt pathways, promoting the early collagen metabolism imbalance after CABG, increasing the collagen deposition in the atrium, and then promoting the early structural reconstruction after CABG and leading to the occurrence of POAF. The result provides a research basis for the accurate prediction and prevention of clinical POAF.

Citation： YAN Ziyi, ZHAO Haibo, HE Chunyue, LIU Yisi. The relationship between miR-3187-5p and postoperative atrial fibrillation after coronary artery bypass grafting: A prospective cohort study. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2023, 30(7): 1038-1047. doi: 10.7507/1007-4848.202206018 Copy

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2.	張凱, 王聯群. 單純非體外循環冠狀動脈旁路移植術后新發房顫術前相關危險因素分析. 天津醫藥, 2018, 46(7): 708-711.
3.	張曉峰. OPCABG術前NLR、PLR與術后新發房顫的相關性研究. 山東大學, 2018.
4.	徐殊. RAAS、Apelin/APJ及MtDNA拷貝數與冠狀動脈旁路移植術后新發房顫的相關性研究. 第四軍醫大學, 2017.
5.	孫殿珉. 心臟能量代謝障礙與CABG術后房顫易感性的研究. 北京協和醫學院, 2015.
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10.	St-Onge S, Perrault LP, Demers P, et al. Pericardial blood as a trigger for postoperative atrial fibrillation after cardiac surgery. Ann Thorac Surg, 2018, 105(1): 321-328.
11.	Xiao L, Liu C, Zhang S, et al. miR-3187-3p enhances migration and invasion by targeting PER2 in head and neck squamous cell carcinomas. J Cancer, 2021, 12(17): 5231-5240.
12.	蘇曉明, 韓喆, 王洋, 等. 心臟術后心房顫動的研究進展. 中國醫藥, 2021, 16(11): 1731-1734.
13.	Gao F, Wang FG, Lyu RR, et al. SLC35E3 identified as a target of novel-m1061-5p via microRNA profiling of patients with cardiovascular disease. Mol Med Rep, 2018, 17(4): 5159-5167.
14.	中華醫學會心血管病學分會介入心臟病學組, 中國醫師協會心血管內科醫師分會血栓防治專業委員會, 中華心血管病雜志編輯委員會. 中國經皮冠狀動脈介入治療指南(2016). 中華心血管病雜志, 2016, 44(5): 382-400.
15.	王乃迪, 張海澄. 《2020ESC心房顫動診斷和管理指南》更新要點解讀. 中國心血管病研究, 2020, 18(11): 966-973.
16.	Zhang ZJ, Song XG, Xie L, et al. Circulating serum exosomal miR-20b-5p and miR-3187-5p as efficient diagnostic biomarkers for early-stage non-small cell lung cancer. Exp Biol Med (Maywood), 2020, 245(16): 1428-1436.
17.	Zhang D, Cai X, Cai S, et al. Long intergenic non-protein coding RNA 01089 weakens tumor proliferation, migration, and invasion by sponging miR-3187-3p in non-small cell lung cancer. Cancer Manag Res, 2020, 12: 12151-12162.
18.	Zhang Z, Huang X, Yang J, et al. Identification and functional analysis of a three-miRNA ceRNA network in hypertrophic scars. J Transl Med, 2021, 19(1): 451.
19.	周羽靖. 體外循環下心臟瓣膜置換術后心包及縱膈引流液量變化規律的臨床觀察. 山西醫科大學, 2019.
20.	李孟婷, 鄧江, 楊丹莉, 等. 心肌肥厚過程中TGF-β和MAPK信號通路間的交互調節. 中國新藥與臨床雜志, 2022, 41(5): 257-263.
21.	陳智慧, 田海萍, 劉艷陽. 心肌纖維化相關通路的研究進展. 醫學綜述, 2022, 28(2): 259-264.
22.	余程. RNA乙酰化酶NAT10調控血管新生內膜形成的作用及機制研究. 福建醫科大學, 2021.
23.	李雪. RRP12在胃癌發生中的作用及機制研究. 山東大學, 2020.
24.	邊帥. GNL3L在食管鱗癌進展中的作用及機制的研究. 吉林大學, 2021.
25.	劉培軍. RBM19、RBM23、RBM34和RBM47在腎透明細胞癌中的臨床意義及對腫瘤發生發展的影響. 華中科技大學, 2017.
26.	魏峰. HEATR1基因與胃癌細胞增殖的相關性研究. 皖南醫學院, 2018.
27.	闕冬冬, 宋旭東. 心房重構在房顫中的作用. 實用心電學雜志, 2021, 30(6): 404-407.
28.	Siontis GCM, Praz F, Lanz J, et al. New-onset arrhythmias following transcatheter aortic valve implantation: A systematic review and meta-analysis. Heart, 2018, 104(14): 1208-1215.
29.	Tao W, Wu F, Tang H, et al. Abstract 18260: Local interleukin-6 and pericardial drainage volume are associated with atrial fibrillation after coronary artery bypass grafting. Circulation, 2011, 21: A18260.
30.	Rader F, Pujara AC, Pattakos G, et al. Perioperative heart-type fatty acid binding protein levels in atrial fibrillation after cardiac surgery. Heart Rhythm, 2013, 10(2): 153-157.
31.	張澤生, 田榮成, 劉抗, 等. 冠狀動脈旁路移植術后并發房顫的研究進展. 贛南醫學院學報, 2019, 39(3): 283-287.

1. 王芯, 陶杰. 心臟直視手術術后房顫的研究進展. 山東醫藥, 2020, 60(13): 93-96.
2. 張凱, 王聯群. 單純非體外循環冠狀動脈旁路移植術后新發房顫術前相關危險因素分析. 天津醫藥, 2018, 46(7): 708-711.
3. 張曉峰. OPCABG術前NLR、PLR與術后新發房顫的相關性研究. 山東大學, 2018.
4. 徐殊. RAAS、Apelin/APJ及MtDNA拷貝數與冠狀動脈旁路移植術后新發房顫的相關性研究. 第四軍醫大學, 2017.
5. 孫殿珉. 心臟能量代謝障礙與CABG術后房顫易感性的研究. 北京協和醫學院, 2015.
6. 趙乃時, 梅舉. 冠狀動脈搭橋術后早期新發房顫的臨床研究進展. 中國心血管病研究, 2014, 12(2): 156-159.
7. 趙清, 楊文濤, 李向東, 等. MicroRNA與心房顫動心房纖維化關系的研究進展. 解放軍醫學院學報, 2021, 42(11): 1220-1224.
8. Feldman A, Moreira DAR, Gun C, et al. Analysis of circulating miR-1, miR-23a, and miR-26a in atrial fibrillation patients undergoing coronary bypass artery grafting surgery. Ann Hum Genet, 2017, 81(3): 99-105.
9. Tsoporis JN, Fazio A, Rizos IK, et al. Increased right atrial appendage apoptosis is associated with differential regulation of candidate microRNAs 1 and 133A in patients who developed atrial fibrillation after cardiac surgery. J Mol Cell Cardiol, 2018, 121: 25-32.
10. St-Onge S, Perrault LP, Demers P, et al. Pericardial blood as a trigger for postoperative atrial fibrillation after cardiac surgery. Ann Thorac Surg, 2018, 105(1): 321-328.
11. Xiao L, Liu C, Zhang S, et al. miR-3187-3p enhances migration and invasion by targeting PER2 in head and neck squamous cell carcinomas. J Cancer, 2021, 12(17): 5231-5240.
12. 蘇曉明, 韓喆, 王洋, 等. 心臟術后心房顫動的研究進展. 中國醫藥, 2021, 16(11): 1731-1734.
13. Gao F, Wang FG, Lyu RR, et al. SLC35E3 identified as a target of novel-m1061-5p via microRNA profiling of patients with cardiovascular disease. Mol Med Rep, 2018, 17(4): 5159-5167.
14. 中華醫學會心血管病學分會介入心臟病學組, 中國醫師協會心血管內科醫師分會血栓防治專業委員會, 中華心血管病雜志編輯委員會. 中國經皮冠狀動脈介入治療指南(2016). 中華心血管病雜志, 2016, 44(5): 382-400.
15. 王乃迪, 張海澄. 《2020ESC心房顫動診斷和管理指南》更新要點解讀. 中國心血管病研究, 2020, 18(11): 966-973.
16. Zhang ZJ, Song XG, Xie L, et al. Circulating serum exosomal miR-20b-5p and miR-3187-5p as efficient diagnostic biomarkers for early-stage non-small cell lung cancer. Exp Biol Med (Maywood), 2020, 245(16): 1428-1436.
17. Zhang D, Cai X, Cai S, et al. Long intergenic non-protein coding RNA 01089 weakens tumor proliferation, migration, and invasion by sponging miR-3187-3p in non-small cell lung cancer. Cancer Manag Res, 2020, 12: 12151-12162.
18. Zhang Z, Huang X, Yang J, et al. Identification and functional analysis of a three-miRNA ceRNA network in hypertrophic scars. J Transl Med, 2021, 19(1): 451.
19. 周羽靖. 體外循環下心臟瓣膜置換術后心包及縱膈引流液量變化規律的臨床觀察. 山西醫科大學, 2019.
20. 李孟婷, 鄧江, 楊丹莉, 等. 心肌肥厚過程中TGF-β和MAPK信號通路間的交互調節. 中國新藥與臨床雜志, 2022, 41(5): 257-263.
21. 陳智慧, 田海萍, 劉艷陽. 心肌纖維化相關通路的研究進展. 醫學綜述, 2022, 28(2): 259-264.
22. 余程. RNA乙酰化酶NAT10調控血管新生內膜形成的作用及機制研究. 福建醫科大學, 2021.
23. 李雪. RRP12在胃癌發生中的作用及機制研究. 山東大學, 2020.
24. 邊帥. GNL3L在食管鱗癌進展中的作用及機制的研究. 吉林大學, 2021.
25. 劉培軍. RBM19、RBM23、RBM34和RBM47在腎透明細胞癌中的臨床意義及對腫瘤發生發展的影響. 華中科技大學, 2017.
26. 魏峰. HEATR1基因與胃癌細胞增殖的相關性研究. 皖南醫學院, 2018.
27. 闕冬冬, 宋旭東. 心房重構在房顫中的作用. 實用心電學雜志, 2021, 30(6): 404-407.
28. Siontis GCM, Praz F, Lanz J, et al. New-onset arrhythmias following transcatheter aortic valve implantation: A systematic review and meta-analysis. Heart, 2018, 104(14): 1208-1215.
29. Tao W, Wu F, Tang H, et al. Abstract 18260: Local interleukin-6 and pericardial drainage volume are associated with atrial fibrillation after coronary artery bypass grafting. Circulation, 2011, 21: A18260.
30. Rader F, Pujara AC, Pattakos G, et al. Perioperative heart-type fatty acid binding protein levels in atrial fibrillation after cardiac surgery. Heart Rhythm, 2013, 10(2): 153-157.
31. 張澤生, 田榮成, 劉抗, 等. 冠狀動脈旁路移植術后并發房顫的研究進展. 贛南醫學院學報, 2019, 39(3): 283-287.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

The relationship between miR-3187-5p and postoperative atrial fibrillation after coronary artery bypass grafting: A prospective cohort study

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