Effect of High Mobility Group Box-1 on The Death Pathways of Pancreatic Acinar Cell in Rat with Severe Acute Pancreatitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 WANGJun , CAIZhi-fang , LIUYao ,  DUIDan-hua

Department of General Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China;

Corresponding?author：

DUIDan-hua, Email: ddh1957@163.com

Keywords：

Severe acute pancreatitis; High mobility group box-1; Oncosis; Flowcytometry

DOI：

10.7507/1007-9424.20140071

Video：

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Objective To explore the effect of the serum high mobility group box-1 (HMGB1) on oncosis of pancreatic acinar cells in the rat with severe acute pancreatitis (SAP). Methods Thirty-two healthy SD rats were randomly divided into 2 groups:sham operation group (SO group, n=8) and SAP group (n=24). Rats of SO group were only flipped the intestinal canal after laparotomy, but rats of SAP group were induced by retrograde injection of 3% sodium taurocholate into bilio-pancreatic duct in addition. Rats of SO group were sacrificed at 6 hours after operation, and rats of SAP group were sacrificed at 6 (SAP-6 hour group, n=8), 12 (SAP-12 hour group, n=8), and 24 hours (SAP-24 hour group, n=8) after operation respectively. Pancreatic tissues were stained by HE to observe pathological changes. Serum HMGB1 was measured by ELISA, and the oncosis percentage of pancreatic acinar cells was examined by flowcytometry. Results Pathological results showed that structural integrity was observed in pancreatic acinar, and occasionally a single inflammatory cell infiltration was observed in rats of SO group. Swelling, interstitial edema, and inflammatory cell infiltration were observed in rats of SAP-6 hour group. Some necrosis of pancreatic acinar cell, stromal vascular congestion, and focal necrosis were observed in rats of SAP-12 hour group and SAP-24 hour group, which the pathological damage were worse over time. Levels of serum HMGB1 and oncosis percentages of pancreatic acinar cells in rats of 3 SAP subgroups were all higher than those of SO group (P < 0.01), and the 2 kinds of indexes both increased over time (P < 0.05). There was positive correlation between concentration of serum HMGB1 and oncosis percentages of pancreatic acinar cells in SAP rat during 24 hours after operation (r=0.846, P < 0.01). Conclusions HMGB1 seems to play an important role in SAP by inducing oncosis of pancreatic acinar cells when inducing inflammatory reaction in rat with SAP.

Citation： WANGJun, CAIZhi-fang, LIUYao, DUIDan-hua. Effect of High Mobility Group Box-1 on The Death Pathways of Pancreatic Acinar Cell in Rat with Severe Acute Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(3): 295-299. doi: 10.7507/1007-9424.20140071 Copy

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2.	王興鵬.重視腸道衰竭在重癥急性胰腺炎發病中的作用[J].中華消化雜志, 2002, 22(1):5-6.
3.	李磊, 王興鵬.炎癥反應的晚期介質HMGB1[J].國外醫學:生理、病理科學與臨床分冊, 2004, 24(6):567-568.
4.	劉旺華, 李花, 肖獻忠.高遷移率族蛋白B-1與炎癥關系的研究進展[J].中國病理生理雜志, 2008, 24(8):1656-1660.
5.	Gdynia G, Keith M, Kopitz J, et al. Danger signaling protein HMGB1 induces a distinct form of cell death accompanied by formation of giant mitochondria[J]. Cancer Res, 2010, 70(21):8558-8568.
6.	郎潔, 張培建.細胞凋亡在急性胰腺炎發病機制中所起作用的研究進展[J].中國普通外科雜志, 2008, 17(9):914-916.
7.	任成山, 錢桂生.細胞凋亡和脹亡及其與多器官功能障礙綜合征關系的研究進展[J].中國危重病急救醫學, 2005, 17(4):247-250.
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9.	Aho HJ, Koskensalo SM, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholate-induced acute haemorrhagic pancreatitis[J]. Scand J Gastroenterol, 1980, 15(4):411-416.
10.	王連才, 馬清涌, 陳香麗.一種改進的簡便經濟的大鼠胰腺腺泡細胞分離提純方法[J].第四軍醫大學學報, 2008, 29(3):220-222.
11.	司徒鎮強, 吳軍正.細胞培養[M].西安:世界圖書出版西安公司, 2007:58-59.
12.	Majno G, Joris I. Apoptosis, oncosis, and necrosis. An overview of cell death[J]. Am J Pathol, 1995, 146(1):3-15.
13.	Trump BF, Berezesky IK, Chang SH, et al. The pathways of cell death:oncosis, apoptosis, and necrosis[J]. Toxicol Pathol, 1997, 25(1):82-88.
14.	Shinoura N, Yoshida Y, Nishimura M, et al. Expression level of Bcl-2 determines anti-or proapoptotic function[J]. Cancer Res, 1999, 59(16):4119-4128.
15.	Alvarez P, Alvarado C, Puerto M, et al. Improvement of leukocyte functions in prematurely aging mice after five weeks of diet supplementation with polyphenol-rich cereals[J]. Nutrition, 2006, 22(9):913-921.
16.	Bhatia M. Apoptosis versus necrosis in acute pancreatitis[J]. Am J Physiol Gastrointest Liver Physiol, 2004, 286(2):G189-G196.
17.	Stolte M, Haas L, Wamwayi HM, et al. Induction of apoptotic cellular death in lymphatic tissues of cattle experimentally infected with different strains of rinderpest virus[J]. J Comp Pathol, 2002, 127(1):14-21.
18.	尚東, 關鳳林, 楊佩滿, 等.胰腺腺泡細胞凋亡在大鼠急性胰腺炎病程中的作用及凋亡調控基因的表達[J].肝膽胰外科雜志, 2001, 13(3):152-155.
19.	Ziangirova GG, Antonova OV. The causes of necrobiosis and apoptosis of corneal epithelial cells during primary acquired keratoconus[J]. Izv Akad Nauk Ser Biol, 2002, 16(5):517-521.
20.	沈美琴, 夏敏.晚期炎癥因子介質高遷移率族蛋白1與重癥急性胰腺炎的關系的研究進展[J].胰腺病學雜志, 2009, 19(6):371-373.
21.	Scaffidi P, Misteli T, Bianchi ME. Release of chromatin protein HMGB1 by necrotic cells triggers inflammation[J]. Nature, 2002, 418(6894):191-195.
22.	朱峰, 龍錦, 何忠野, 等.急性壞死性胰腺炎大鼠胰腺高遷移率族蛋白-1的表達及意義[J].中國普通外科雜志, 2007, 16(5):418-421.
23.	戴振華, 王亮, 孫偉, 等. HMGB1在鑒別過氧化氫誘導心肌細胞凋亡和死亡中作用的研究[J].南京醫科大學學報:自然科學版, 2007, 27(9):956-959.
24.	楊智勇, 王春友, 熊炯炘, 等.重癥急性胰腺炎大鼠血清高遷移率族蛋白-1水平的時相變化及意義[J].華中科技大學學報:醫學版, 2004, 33(4):466-468.

1. ?Hyv?nen MT, Herzig KH, Sinervirta R, et al. Activated polya-mine catabolism in acute pancreatitis:alpha-methylated polyamine analogues prevent trypsinogen activation and pancreatitis-associated mortality[J]. Am J Pathol, 2006, 168(1):115-122.
2. 王興鵬.重視腸道衰竭在重癥急性胰腺炎發病中的作用[J].中華消化雜志, 2002, 22(1):5-6.
3. 李磊, 王興鵬.炎癥反應的晚期介質HMGB1[J].國外醫學:生理、病理科學與臨床分冊, 2004, 24(6):567-568.
4. 劉旺華, 李花, 肖獻忠.高遷移率族蛋白B-1與炎癥關系的研究進展[J].中國病理生理雜志, 2008, 24(8):1656-1660.
5. Gdynia G, Keith M, Kopitz J, et al. Danger signaling protein HMGB1 induces a distinct form of cell death accompanied by formation of giant mitochondria[J]. Cancer Res, 2010, 70(21):8558-8568.
6. 郎潔, 張培建.細胞凋亡在急性胰腺炎發病機制中所起作用的研究進展[J].中國普通外科雜志, 2008, 17(9):914-916.
7. 任成山, 錢桂生.細胞凋亡和脹亡及其與多器官功能障礙綜合征關系的研究進展[J].中國危重病急救醫學, 2005, 17(4):247-250.
8. 楚江濤, 薛東波.急性胰腺炎腺泡細胞的死亡方式[J].中國現代普通外科進展, 2012, 15(4):302-304.
9. Aho HJ, Koskensalo SM, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholate-induced acute haemorrhagic pancreatitis[J]. Scand J Gastroenterol, 1980, 15(4):411-416.
10. 王連才, 馬清涌, 陳香麗.一種改進的簡便經濟的大鼠胰腺腺泡細胞分離提純方法[J].第四軍醫大學學報, 2008, 29(3):220-222.
11. 司徒鎮強, 吳軍正.細胞培養[M].西安:世界圖書出版西安公司, 2007:58-59.
12. Majno G, Joris I. Apoptosis, oncosis, and necrosis. An overview of cell death[J]. Am J Pathol, 1995, 146(1):3-15.
13. Trump BF, Berezesky IK, Chang SH, et al. The pathways of cell death:oncosis, apoptosis, and necrosis[J]. Toxicol Pathol, 1997, 25(1):82-88.
14. Shinoura N, Yoshida Y, Nishimura M, et al. Expression level of Bcl-2 determines anti-or proapoptotic function[J]. Cancer Res, 1999, 59(16):4119-4128.
15. Alvarez P, Alvarado C, Puerto M, et al. Improvement of leukocyte functions in prematurely aging mice after five weeks of diet supplementation with polyphenol-rich cereals[J]. Nutrition, 2006, 22(9):913-921.
16. Bhatia M. Apoptosis versus necrosis in acute pancreatitis[J]. Am J Physiol Gastrointest Liver Physiol, 2004, 286(2):G189-G196.
17. Stolte M, Haas L, Wamwayi HM, et al. Induction of apoptotic cellular death in lymphatic tissues of cattle experimentally infected with different strains of rinderpest virus[J]. J Comp Pathol, 2002, 127(1):14-21.
18. 尚東, 關鳳林, 楊佩滿, 等.胰腺腺泡細胞凋亡在大鼠急性胰腺炎病程中的作用及凋亡調控基因的表達[J].肝膽胰外科雜志, 2001, 13(3):152-155.
19. Ziangirova GG, Antonova OV. The causes of necrobiosis and apoptosis of corneal epithelial cells during primary acquired keratoconus[J]. Izv Akad Nauk Ser Biol, 2002, 16(5):517-521.
20. 沈美琴, 夏敏.晚期炎癥因子介質高遷移率族蛋白1與重癥急性胰腺炎的關系的研究進展[J].胰腺病學雜志, 2009, 19(6):371-373.
21. Scaffidi P, Misteli T, Bianchi ME. Release of chromatin protein HMGB1 by necrotic cells triggers inflammation[J]. Nature, 2002, 418(6894):191-195.
22. 朱峰, 龍錦, 何忠野, 等.急性壞死性胰腺炎大鼠胰腺高遷移率族蛋白-1的表達及意義[J].中國普通外科雜志, 2007, 16(5):418-421.
23. 戴振華, 王亮, 孫偉, 等. HMGB1在鑒別過氧化氫誘導心肌細胞凋亡和死亡中作用的研究[J].南京醫科大學學報:自然科學版, 2007, 27(9):956-959.
24. 楊智勇, 王春友, 熊炯炘, 等.重癥急性胰腺炎大鼠血清高遷移率族蛋白-1水平的時相變化及意義[J].華中科技大學學報:醫學版, 2004, 33(4):466-468.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of High Mobility Group Box-1 on The Death Pathways of Pancreatic Acinar Cell in Rat with Severe Acute Pancreatitis

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