Experimental Study on The Cell Culture and Secretory Function of Human Parathyroid Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 SUNHai-chen , LIUShuang , CUIYe-qing , WANGXiao-hui ,  LUOBin

Departmengt of General Surgery, Xuanwu Hospital of Capital Medical University, Beijing 100053, China;

Corresponding?author：

LUOBin, Email: binluobj@sina.Com

Keywords：

Parathyroid cell; Cell culture; Cell transplantation; Parathyroid hormone

DOI：

10.7507/1007-9424.20140269

Video：

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Objective To investigate the way to culture human parathyroid cells and to investigate its secretory function. Methods After digested by collagenase, parathyroid cells were isolated to get the original generation cells, then the cells were cultured and passaged, and morphological changes of original generation cells and passage cells were observed on every day. The parathyroid hormone(PTH) level secreted by the original generation cells and passage cells were measured on the 1^st, 5^th, 10^th, 15^th, and 20^th day(original generation cells only) respectively. Results The cellular morphology was complete after digestion. On the 2^nd day, most of the parathyroid cells had adhered and spreaded, on the 3^rd day, all cells had spread. There was no very obvious changes on these cells after cultured for 4-15 days. From 16 to 20 days, some parathyroid cells went senescence. On the 1^st day, all of the passage cells, which were fusiform and little bigger than those of the original generation cells, had adhered and spreaded. From 2 to 15 days, there was no very obvious changes. The concentration of PTH in original generation cells begin to decreased significantly on the 10^th day (P < 0.01). The concentration of PTH in passage cells were all lower than those of original generation cells at the same corresponding time, but there were no significant difference on the PTH level on 5^th day and 1^st day, 10^th day and 5^th day, 15^th day and 10^th day in passage cells (P > 0.05). Conclusion Parathyroid cells which were cultured within 10 days possess well morphologic structure and have the strongest secretory function. Although the passage cells still possess secretory function, it is greatly inferior to original generation cells. At last, we consider that original generation cells cultured within 10 days can be regarded as the source of allogeneic cell transplantation.

Citation： SUNHai-chen, LIUShuang, CUIYe-qing, WANGXiao-hui, LUOBin. Experimental Study on The Cell Culture and Secretory Function of Human Parathyroid Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(9): 1120-1123. doi: 10.7507/1007-9424.20140269 Copy

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2.	鄂征. 組織培養和分子細胞學技術[M]. 北京:北京出版社, 1994:87-89.
3.	馬冬冬, 季冠芳, 徐志惠, 等. 甲狀旁腺激素PTH (1-34)的二級結構及其功能研究[J]. 天津醫科大學學報, 2002, 8(3):314-315, 373.
4.	李成, 張偉輝. 甲狀旁腺功能低下癥治療現狀[J]. 哈爾濱醫科大學學報, 2004, 38(4):405-407.
5.	高恒元, 周冬仙, 劉新杰, 等. Ⅰ期甲狀旁腺自體移植的臨床研究進展[J]. 中國醫師進修雜志, 2010, 33(8):73-74.
6.	涂剛, 羅浩軍, 姚榛祥. 甲狀旁腺移植現狀[J]. 內分泌外科雜志, 2008, 2(5):331-334, 351.
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8.	高慶軍, 趙代偉, 王南鵬, 等. 同種異體甲狀旁腺組織移植治療甲狀旁腺功能低下的實驗研究[J]. 中國普外基礎與臨床雜志, 2012, 19(10):1079-1084.
9.	Schachter P, Christy MD, Leight GS, et al. Function in athymic nude mice of parathyroid heterografts from patients with primary hyperparathyroidism and secondary hyperparathyroidism[J]. Surgery, 1990, 108(6):1040-1046.
10.	Kadowaki MH, Fulton N, Schark C, et al. Difficulties of parathyroidectomy after previous thyroidectomy[J]. Surgery, 1989, 106(6):1018-1024.
11.	Tsuji K, Fuchinoue S, Kai K, et al. Culture of human parathyroid cells for transplantation[J]. Transplant Proc, 1999, 31(7):2697.
12.	宋純, 宋一民, 武林楓, 等. 人胚甲狀旁腺細胞移植治療甲狀旁腺功能低下癥[J]. 中華外科雜志, 2000, 38(9):690-692.
13.	段秀慶, 宋純, 王江濱, 等. 大鼠甲狀旁腺細胞經培養后再移植的實驗研究[J]. 中國普外基礎與臨床雜志, 2004, 11(6):486-488.
14.	馬鳳賢, 王江濱, 張亞仙. 甲狀旁腺功能低下患者細胞移植及其護理[J]. 護理學雜志, 2005, 20(14):42-43.
15.	楊冰, 王梅. 高磷對大鼠甲狀旁腺組織及原代培養細胞的刺激作用[J]. 中國血液凈化, 2008, 7(6):317-320.
16.	宋純, 張偉輝, 宋春芳, 等. 體外培養甲狀旁腺細胞形態與功能的實驗研究[J]. 局解手術學雜志, 1997, 6(4):42-44.
17.	宋一民, 周毅, 武林楓, 等. 人胚甲狀旁腺細胞移植供體胎齡的選擇[J]. 傷殘醫學雜志, 2001, 9(1):4-6.
18.	邰升, 宋一民, 宋純, 等. 人胎甲狀旁腺細胞培養形態與功能的研究[J]. 哈爾濱醫科大學學報, 2000, 34(6):409-411.
19.	段秀慶, 李永寧, 宋純. 大鼠甲狀旁腺細胞體外培養及功能的實驗研究[J]. 中國現代普通外科進展, 2008, 11(5):389-391.
20.	Picariello L, Benvenuti S, Recenti R, et al. Microencapsulation of human parathyroid cells:an "in vitro" study[J]. J Surg Res, 2001, 96(1):81-89.
21.	唐功耀, 張凌. 微囊免疫隔離技術在醫學中的應用[J]. 臨床藥物治療雜志, 2012, 10(2):27-30, 40.
22.	楊曉昀, 陳兒同, 周燕, 等. 微囊化技術在醫學領域中的應用與進展[J]. 中國組織工程研究與臨床康復, 2010, 14(29):5483-5488.
23.	劉春蓉, 苗軍, 余鵬, 等. 微囊化人工細胞技術及其應用[J]. 中國組織工程研究與臨床康復, 2007, 11(5):949-952.
24.	沈陽, 湯亭亭. 細胞微囊技術在再生醫學領域的應用[J]. 國際骨科學雜志, 2011, 32(2):81-82, 86.

1. 黃家駟. 外科學[M]. 北京:人民衛生出版社, 1994:887.
2. 鄂征. 組織培養和分子細胞學技術[M]. 北京:北京出版社, 1994:87-89.
3. 馬冬冬, 季冠芳, 徐志惠, 等. 甲狀旁腺激素PTH (1-34)的二級結構及其功能研究[J]. 天津醫科大學學報, 2002, 8(3):314-315, 373.
4. 李成, 張偉輝. 甲狀旁腺功能低下癥治療現狀[J]. 哈爾濱醫科大學學報, 2004, 38(4):405-407.
5. 高恒元, 周冬仙, 劉新杰, 等. Ⅰ期甲狀旁腺自體移植的臨床研究進展[J]. 中國醫師進修雜志, 2010, 33(8):73-74.
6. 涂剛, 羅浩軍, 姚榛祥. 甲狀旁腺移植現狀[J]. 內分泌外科雜志, 2008, 2(5):331-334, 351.
7. 曹利平, 汪亮. 甲狀旁腺移植現狀及趨勢[J]. 中國實用外科雜志, 2008, 28(3):227-229.
8. 高慶軍, 趙代偉, 王南鵬, 等. 同種異體甲狀旁腺組織移植治療甲狀旁腺功能低下的實驗研究[J]. 中國普外基礎與臨床雜志, 2012, 19(10):1079-1084.
9. Schachter P, Christy MD, Leight GS, et al. Function in athymic nude mice of parathyroid heterografts from patients with primary hyperparathyroidism and secondary hyperparathyroidism[J]. Surgery, 1990, 108(6):1040-1046.
10. Kadowaki MH, Fulton N, Schark C, et al. Difficulties of parathyroidectomy after previous thyroidectomy[J]. Surgery, 1989, 106(6):1018-1024.
11. Tsuji K, Fuchinoue S, Kai K, et al. Culture of human parathyroid cells for transplantation[J]. Transplant Proc, 1999, 31(7):2697.
12. 宋純, 宋一民, 武林楓, 等. 人胚甲狀旁腺細胞移植治療甲狀旁腺功能低下癥[J]. 中華外科雜志, 2000, 38(9):690-692.
13. 段秀慶, 宋純, 王江濱, 等. 大鼠甲狀旁腺細胞經培養后再移植的實驗研究[J]. 中國普外基礎與臨床雜志, 2004, 11(6):486-488.
14. 馬鳳賢, 王江濱, 張亞仙. 甲狀旁腺功能低下患者細胞移植及其護理[J]. 護理學雜志, 2005, 20(14):42-43.
15. 楊冰, 王梅. 高磷對大鼠甲狀旁腺組織及原代培養細胞的刺激作用[J]. 中國血液凈化, 2008, 7(6):317-320.
16. 宋純, 張偉輝, 宋春芳, 等. 體外培養甲狀旁腺細胞形態與功能的實驗研究[J]. 局解手術學雜志, 1997, 6(4):42-44.
17. 宋一民, 周毅, 武林楓, 等. 人胚甲狀旁腺細胞移植供體胎齡的選擇[J]. 傷殘醫學雜志, 2001, 9(1):4-6.
18. 邰升, 宋一民, 宋純, 等. 人胎甲狀旁腺細胞培養形態與功能的研究[J]. 哈爾濱醫科大學學報, 2000, 34(6):409-411.
19. 段秀慶, 李永寧, 宋純. 大鼠甲狀旁腺細胞體外培養及功能的實驗研究[J]. 中國現代普通外科進展, 2008, 11(5):389-391.
20. Picariello L, Benvenuti S, Recenti R, et al. Microencapsulation of human parathyroid cells:an "in vitro" study[J]. J Surg Res, 2001, 96(1):81-89.
21. 唐功耀, 張凌. 微囊免疫隔離技術在醫學中的應用[J]. 臨床藥物治療雜志, 2012, 10(2):27-30, 40.
22. 楊曉昀, 陳兒同, 周燕, 等. 微囊化技術在醫學領域中的應用與進展[J]. 中國組織工程研究與臨床康復, 2010, 14(29):5483-5488.
23. 劉春蓉, 苗軍, 余鵬, 等. 微囊化人工細胞技術及其應用[J]. 中國組織工程研究與臨床康復, 2007, 11(5):949-952.
24. 沈陽, 湯亭亭. 細胞微囊技術在再生醫學領域的應用[J]. 國際骨科學雜志, 2011, 32(2):81-82, 86.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Experimental Study on The Cell Culture and Secretory Function of Human Parathyroid Cells

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