Establishment and Evaluation of Type 2 Diabetes SD Rat Model_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 SHAOJun-wei ,  CAIXun , MADan-dan , HUYi-lin , ZHANGYi

Department of General Surgery, Wuhan General Hospital of Guangzhou Command, Wuhan 430070, Hubei Province, China;

Corresponding?author：

CAIXun, Email: caiwenqian@sina.com

Keywords：

Type 2 diabetes; Rat model; Streptozotocin; High fat and glucose diet

DOI：

10.7507/1007-9424.20140291

Video：

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Objective To evaluate the influencing factors and explore a better method of making rat model of type 2 diabetes by high fat and sugar diet and streptozotocin(STZ) injection. Methods Sixty SPF grade of 6 weeks male SD rats were fed with high fat and glucose diet by 4 weeks and then randomly divided into 3 groups, the control group rats(n=20) were injected citric acid by 50 mg/kg and fed with normal diet, and the diabetes mellitus group rats were further divided into 2 subgroups by the different doses of STZ:the rats of diabetes mellitus model group 1(n=20) were injected by 50 mg/kg, while the rats of diabetes mellitus model group 2(n=20) were injected by 35 mg/kg. The diabetes mellitus model group rats were fed with high fat and glucose diet continually. The fasting blood glucose(FBG) were measured on day 3, 7, 10, and 14, respectively. The success model rate(blood glucose > 16.7 mmol/L after 14 days) and the mortality rate were calculated. Meanwhile fasting serum insulin level(FSI), total serum cholesterol(TC), and triglyceride(TG) were measured. Results Compared with the control group, the levels of FBG, FSI, TC, and TG were increased significantly in the diabetes mellitus model group 1 and 2(P < 0.05). And insulin sensitivity was worsen markedly(P < 0.05). But the diabetes mellitus model group 2 had higher success rate of making model(85% vs. 75%) and lower mortality(0 vs. 25%), P < 0.05. Conclusion Rat model of type 2 diabetes induced by 4 weeks of high fat and sugar feeding and 35 mg/kg STZ injection has high morbidity, strongly security, and stable features.

Citation： SHAOJun-wei, CAIXun, MADan-dan, HUYi-lin, ZHANGYi. Establishment and Evaluation of Type 2 Diabetes SD Rat Model. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(10): 1212-1215. doi: 10.7507/1007-9424.20140291 Copy

1.	Wild S, Roglic G, Green A, et al. Global prevalence of diabetes:estimates for the year 2000 and projections for 2030[J]. Diabetes Care, 2004, 27(5):1047-1053.
2.	Expert Committee on the Diagnosis and Classification of Diabetes Mellitus. Report of the expert committee on the diagnosis and classification of diabetes mellitus[J]. Diabetes Care, 2003, 26 Suppl 1:S5-S20.
3.	楊架林, 李果, 劉優萍, 等.長期高脂飲食加小劑量鏈脲佐菌素建立人類普通2型糖尿病大鼠模型的研究[J].中國實驗動物學報, 2003, 11(3):138-141.
4.	郭嘯華, 劉志紅, 李恒, 等.高糖高脂飲食誘導的2型糖尿病大鼠模型及其腎病特點[J].中國糖尿病雜志, 2002, 10(5):290-294.
5.	楊李, 唐瑛, 陳芳, 等.芪蓮湯顆粒長期干預對大鼠糖尿病并發癥的影響[J].醫藥導報, 2011, 30(5):563-565.
6.	張玉領, 陳培, 劉家秀, 等. 2型糖尿病大鼠模型的建立[J].現代預防醫學, 2012, 39(15):3922-3924.
7.	汪園園, 歐斌, 李蘇華, 等.利用高糖高脂飲食建立胰島素抵抗大鼠模型[J].深圳中西醫結合雜志, 2011, 21(1):13-14.
8.	Goto Y, Kakizaki M, Masaki N. Production of spontaneous diabetic rats by repetition of selective breeding[J]. Tohoku J Exp Med, 1976, 119(1):85-90.
9.	Srinivasan K, Ramarao P. Anamal models in type 2 diabetes research:an overview[J]. Indian J Med Res, 2007, 125(3):451-472.
10.	Al-Khalifa A, Mathew TC, Al-Zaid NS, et al. Low carbohydrate ketogenic diet prevents the induction of diabetes using streptozotocin in rats[J]. Exp Toxicol Pathol, 2011, 63(7-8):663-669.
11.	Kim CH, Youn JH, Park JY, et al. Effects of high-fat diet and exercise training on intracellular glucose metabolism in rats[J]. Am J Physiol Endo Metab, 2000, 278(6):977-984.
12.	張均田主編.現代藥理學實驗方法[M].北京:北京醫科大學中國協和醫科大學聯合出版社, 1998:981-988.
13.	沈亞非, 徐焱成.鏈脲佐菌素誘導實驗性糖尿病模型建立的研究[J].實用診斷與治療雜志, 2005, 19(2):79-80.
14.	Winzell MS, Ahrén B. The high-fat diet-fed mouse:a model for studying mechanisms and treatment of impaired glucose tolerance and type 2 diabetes[J]. Diabetes, 2004, 53 Suppl 3:S215-S219.
15.	Chalkley SM, Hettiarachchi M, Chisholm DJ, et al. Long-term high-fat feeding leads to severe insulin resistance but not diabetes in Wistar rats[J]. Am J Physiol Endocrinol Metab, 2002, 282(6):1231-1238.
16.	董佳生, 徐華, 王露萍, 等. SD大鼠I型糖尿病動物模型的建立[J].山西醫藥雜志, 2009, 38(3):218-220.
17.	Wilkinson-Berka JL, Kelly DJ, Koerner SM, et al. ALT-946 and aminoguanidine, inhibitors of advanced glycation, improve seven nephropathyin the diabetic transgenic (mREN-2) 27 rats[J]. Diabetes, 2002, 51(11):3283-3289.
18.	孫兆峰, 王利, 夏作理. 2型糖尿病動物模型研究概要[J].中國微循環, 2008, 12(3):187-189.
19.	Zhou YS, Gao Y, Guo XH, et al. Effect of timely insulin treatment on protection ofβcells in a rat model of type 2 diabetes mellitus[J]. Chin Med J (Engl), 2004, 117(10):1523-1529.
20.	施紅, 金國琴, 余文珍.誘導構建最佳類似人類2型糖尿病大鼠的造模方式[J].中國臨床康復, 2005, 9(39):69-71.
21.	胡玉煥, 寧亞功, 肖燕.高熱量飲食聯合鏈脲佐菌素建立2型糖尿病大鼠模型的影響因素[J].江西中醫學院學報, 2013, 25(1):70-73.
22.	Morimoto S, Mendoza-Rodriguez CA, Hiriart M, et al. Protective effect of testosterone on early apoptotic damage induced by streptozotocin in rat pancreas[J]. J Endocrinol, 2005, 187(2):217-224.
23.	蔣升, 謝自敬, 張莉.鏈脲佐菌素誘導1型糖尿病大鼠模型穩定性觀察[J].中國比較醫學雜志, 2006, 16(1):16-18.
24.	Masiello P, De Paoli A, Bergamini E. Age-dependent changes in the sensitivity of the rat to a diabetoenic agent (streptozotocin)[J]. Endocrinology, 1975, 96(3):787-789.

1. Wild S, Roglic G, Green A, et al. Global prevalence of diabetes:estimates for the year 2000 and projections for 2030[J]. Diabetes Care, 2004, 27(5):1047-1053.
2. Expert Committee on the Diagnosis and Classification of Diabetes Mellitus. Report of the expert committee on the diagnosis and classification of diabetes mellitus[J]. Diabetes Care, 2003, 26 Suppl 1:S5-S20.
3. 楊架林, 李果, 劉優萍, 等.長期高脂飲食加小劑量鏈脲佐菌素建立人類普通2型糖尿病大鼠模型的研究[J].中國實驗動物學報, 2003, 11(3):138-141.
4. 郭嘯華, 劉志紅, 李恒, 等.高糖高脂飲食誘導的2型糖尿病大鼠模型及其腎病特點[J].中國糖尿病雜志, 2002, 10(5):290-294.
5. 楊李, 唐瑛, 陳芳, 等.芪蓮湯顆粒長期干預對大鼠糖尿病并發癥的影響[J].醫藥導報, 2011, 30(5):563-565.
6. 張玉領, 陳培, 劉家秀, 等. 2型糖尿病大鼠模型的建立[J].現代預防醫學, 2012, 39(15):3922-3924.
7. 汪園園, 歐斌, 李蘇華, 等.利用高糖高脂飲食建立胰島素抵抗大鼠模型[J].深圳中西醫結合雜志, 2011, 21(1):13-14.
8. Goto Y, Kakizaki M, Masaki N. Production of spontaneous diabetic rats by repetition of selective breeding[J]. Tohoku J Exp Med, 1976, 119(1):85-90.
9. Srinivasan K, Ramarao P. Anamal models in type 2 diabetes research:an overview[J]. Indian J Med Res, 2007, 125(3):451-472.
10. Al-Khalifa A, Mathew TC, Al-Zaid NS, et al. Low carbohydrate ketogenic diet prevents the induction of diabetes using streptozotocin in rats[J]. Exp Toxicol Pathol, 2011, 63(7-8):663-669.
11. Kim CH, Youn JH, Park JY, et al. Effects of high-fat diet and exercise training on intracellular glucose metabolism in rats[J]. Am J Physiol Endo Metab, 2000, 278(6):977-984.
12. 張均田主編.現代藥理學實驗方法[M].北京:北京醫科大學中國協和醫科大學聯合出版社, 1998:981-988.
13. 沈亞非, 徐焱成.鏈脲佐菌素誘導實驗性糖尿病模型建立的研究[J].實用診斷與治療雜志, 2005, 19(2):79-80.
14. Winzell MS, Ahrén B. The high-fat diet-fed mouse:a model for studying mechanisms and treatment of impaired glucose tolerance and type 2 diabetes[J]. Diabetes, 2004, 53 Suppl 3:S215-S219.
15. Chalkley SM, Hettiarachchi M, Chisholm DJ, et al. Long-term high-fat feeding leads to severe insulin resistance but not diabetes in Wistar rats[J]. Am J Physiol Endocrinol Metab, 2002, 282(6):1231-1238.
16. 董佳生, 徐華, 王露萍, 等. SD大鼠I型糖尿病動物模型的建立[J].山西醫藥雜志, 2009, 38(3):218-220.
17. Wilkinson-Berka JL, Kelly DJ, Koerner SM, et al. ALT-946 and aminoguanidine, inhibitors of advanced glycation, improve seven nephropathyin the diabetic transgenic (mREN-2) 27 rats[J]. Diabetes, 2002, 51(11):3283-3289.
18. 孫兆峰, 王利, 夏作理. 2型糖尿病動物模型研究概要[J].中國微循環, 2008, 12(3):187-189.
19. Zhou YS, Gao Y, Guo XH, et al. Effect of timely insulin treatment on protection ofβcells in a rat model of type 2 diabetes mellitus[J]. Chin Med J (Engl), 2004, 117(10):1523-1529.
20. 施紅, 金國琴, 余文珍.誘導構建最佳類似人類2型糖尿病大鼠的造模方式[J].中國臨床康復, 2005, 9(39):69-71.
21. 胡玉煥, 寧亞功, 肖燕.高熱量飲食聯合鏈脲佐菌素建立2型糖尿病大鼠模型的影響因素[J].江西中醫學院學報, 2013, 25(1):70-73.
22. Morimoto S, Mendoza-Rodriguez CA, Hiriart M, et al. Protective effect of testosterone on early apoptotic damage induced by streptozotocin in rat pancreas[J]. J Endocrinol, 2005, 187(2):217-224.
23. 蔣升, 謝自敬, 張莉.鏈脲佐菌素誘導1型糖尿病大鼠模型穩定性觀察[J].中國比較醫學雜志, 2006, 16(1):16-18.
24. Masiello P, De Paoli A, Bergamini E. Age-dependent changes in the sensitivity of the rat to a diabetoenic agent (streptozotocin)[J]. Endocrinology, 1975, 96(3):787-789.

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Establishment and Evaluation of Type 2 Diabetes SD Rat Model

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