Protective and Therapeutic Effects of Curcumin Combined with Rhodiola on Rats with Severe Acute Pancreatitis Associated Renal Injury_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 LIZhi-ling ^1,2 , ZHANGDong ¹ ,  LIUJiang-wei ¹ , WANGHao ¹ , GAOTing ¹ , LUKai-bai ¹ , ZHAOHong-yan ¹

1. Department of Hepatobiliary Surgery, Urumuqi General Hospital of Lanzhou Military Region, Urumuqi 830000, Xinjiang Uygur Autonomous Region, China;
2. Department of General Surgury, The Second People's Hospital of Liangshan Prefecture, Xichang 615000, Sichuan Province, China;

Corresponding?author：

LIUJiang-wei, Email: ljw273@sohu.com

Keywords：

Severe acute pancreatitis; Renal injury; Curcumin; Rhodiola; Inducible nitric oxide synthase; Rat

DOI：

10.7507/1007-9424.20140330

Video：

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Objective To investigate the effect of Curcumin combined with Rhodiola on rats with severe acute pancreatitis (SAP) associated renal injury and explore the possible mechanisms. Methods A total of 24 rats were randomly divided into SAP with renal injury group (SAP group, n=8), Curcumin group (n=8), Curcumin combined with Rhodiola group (n=8).The SAP group was given 1.5 mL saline through intragastric administration before operation while the Curcumin group was fed with same amount of Curcumin diluent.The Curcumin combined with Rhodiola group was given 1.5 mL Curcumin diluent through intragastric administration and 6 g/kg Rhodiola diluent through intraperitoneal injection before operation.The pancreas and pancreatic tail-segment was dissociated and the head of pancreas were occluded in rats to make the model, blood vessel forceps was loosed after three hours.All the rats were sacrificed at 18 h after modeling.The levels of serum amylase, creatinine, blood urea nitrogen were detected and pathological changes of pancreas and the left kidney were observed under the light microscope.The cell apoptosis was analyzed using TUNEL staining.The serum levels of interleukin (IL)-1β, IL-6, and IL-10 among the three groups were detected by enzyme-linked immunosorbent assay.The expression of inducible nitric oxide synthase (iNOS) mRNA in the right kidney was detected by real-time polymerase chain reaction.The superoxide dismutase (SOD) activity of the renal tissue was determined by hydroxylamine method. Results Compared with the SAP group, the levels of serum amylase, creatinine, blood urea nitrogen, IL-1β, IL-6, the cell apoptosis index, and the expression of iNOS mRNA were significantly decreased, the serum level of IL-10 and the activity of SOD were significantly increased (P < 0.05), the pancreas and the kidney damaged more slightly in the Curcumin group and Curcumin combined with Rhodiola group.Compared with the Curcumin group, the above situations were more better in the Curcumin combined with Rhodiola group. Conclusions Curcumin combined with Rhodiola has a better protective effect on SAP associated renal injury.It might be through inhibiting the expressions of IL-1β, IL-6, stimulating the expression of IL-10, down-regulating the expression of iNOS mRNA, and improving the activity of SOD.It could reduce the cell apoptosis and necrosis of the kidney and improve the ability of the kidney to tolerate hypoxia.

Citation： LIZhi-ling, ZHANGDong, LIUJiang-wei, WANGHao, GAOTing, LUKai-bai, ZHAOHong-yan. Protective and Therapeutic Effects of Curcumin Combined with Rhodiola on Rats with Severe Acute Pancreatitis Associated Renal Injury. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2014, 21(11): 1377-1383. doi: 10.7507/1007-9424.20140330 Copy

1.	?Bennett M, Dent CL, Ma Q, et al.Urine NGAL predicts severity of acute kidney injury after cardiac surgery:a prospective study[J].Clin J Am Soc Nephrol, 2008, 3(3):665-673.
2.	李敏利, 張曉華, 朱人敏, 等.抑制JAK/STAT3信號通路對重癥急性胰腺炎腎損傷的保護作用[J].世界華人消化雜志, 2011, 19(32):3297-3301.
3.	張瑩, 石承先, 李玉祥, 等.丹參對重癥急性胰腺炎大鼠誘導型一氧化氮合成酶mRNA的表達與器官損傷的影響[J].中國中西醫結合雜志, 2005, 25(11):1012-1015.
4.	吳新正, 何迎春, 劉紅萍, 等.紅景天復方對小鼠免疫、耐缺氧和抗疲勞能力的影響[J].湖南中醫藥大學學報, 2008, 28(1):29-31.
5.	劉江正, 海春旭, 梁欣.紅景天醇提物對乙醇誘導QZG細胞氧化損傷的保護作用[J].癌變·畸變·突變, 2011, 23(2):81-86.
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7.	Kozhukhar AV, Yasinska IM, Sumbayev VV.Nitric oxide inhibits HIF-1alpha protein accumulation under hypoxic conditions:implication of 2-oxoglutarate and iron[J].Biochimie, 2006, 88(5):411-418.
8.	Lieberthal W.Biology of ischemic and toxic renal tubular cell injury:role of nitric oxide and the inflammatory response[J].Curr Opin Nephrol Hypertens, 1998, 7(3):289-295.
9.	劉紅艷, 王海燕, 葉松, 等.姜黃素藥理作用及其機制研究進展[J].中國現代醫學雜志, 2012, 22(6):48-51.
10.	劉靜, 黃麗, 胡曉松, 等.姜黃素對大鼠重癥急性胰腺炎防治作用及其機制[J].中國普通外科雜志, 2012, 21(3):283-289.
11.	王莉, 趙樺.小叢紅景天脂溶性成分及抗菌活性分析[J].中國實驗方劑學雜志, 2012, 18(16):127-131.
12.	Novovic S, Andersen AM, Ersb?ll AK, et al.Proinflammatory cytokines in alcohol or gallstone induced acute pancreatitis[J].JOP, 2009, 10(3):256-262.
13.	王先坤, 詹樂恒, 王世文, 等.IL-10、IL-18在重癥急性胰腺炎肺損傷中的作用[J].中國普外基礎與臨床雜志, 2005, 12(5):443-446.
14.	牛愛麗, 廖青, 張碩, 等.姜黃素對大強度運動大鼠腎損傷的保護作用及其機制[J].湖南師范大學學報:醫學版, 2012, 9(1):36-39.
15.	Guan S, He J, Guo W, et al.Adjuvant effects of salidroside from Rhodiola rosea L.on the immune responses to ovalbumin in mice[J].Immunopharmacol Immunotoxicol, 2011, 33(4):738-743.
16.	Lin SS, Chin LW, Chao PC, et al.In vivo Th1 and Th2 cytokine modulation effects of Rhodiola rosea standardised solution and its major constituent, salidroside[J].Phytother Res, 2011, 25(11):1604-1611.
17.	Moore KW, de Waal Malefyt R, Coffman RL, et al.Interleukin-10 and the interleukin-10 receptor[J].Annu Rev Immunol, 2001, 19(2):683-765.
18.	李兆申, 張文俊, 李維勤, 等.胰腺炎[M].北京:科學出版社, 2010:94-95.
19.	Selvam R, Subramanian L, Gayathri R, et al.The anti-oxidant activity of turmeric (Curcuma longa)[J].J Ethnopharmacol, 1995, 47(2):59-67.
20.	龔曉武, 李炳奇, 劉丹丹, 等.紅景天黃酮提取及其抗氧化活性研究[J].西北林學院學報, 2011, 26(3):136-138.
21.	許晗, 尹偉偉, 丁鼎.高山紅景天總甙抗衰老作用機理的實驗研究[J].浙江中醫藥大學學報, 2011, 35(2):248-249, 252.
22.	金艷玲, 周洋, 王洪海, 等.紅景天苷對大鼠腎臟缺血再灌注損傷的預防與保護作用[J].中藥新藥與臨床藥理, 2010, 21(1):22-25.
23.	馬天翔, 史寧, 陳乾, 等.紅景天中8種成分體外抗氧化作用的比較[J].中國藥理學通報, 2012, 28(9):1224-1228.
24.	張欣, 隋汝波.紅景天對大鼠缺血再灌注后細胞因子的影響[J].中國新藥雜志, 2008, 17(6):472-473, 485.
25.	惠遠見, 王衛星, 余佳, 等.急性胰腺炎大鼠腎臟中聚腺苷二磷酸核糖聚合酶-1 mRNA的表達及意義[J].中國普外基礎與臨床雜志, 2010, 17(3):272-274.

1. ?Bennett M, Dent CL, Ma Q, et al.Urine NGAL predicts severity of acute kidney injury after cardiac surgery:a prospective study[J].Clin J Am Soc Nephrol, 2008, 3(3):665-673.
2. 李敏利, 張曉華, 朱人敏, 等.抑制JAK/STAT3信號通路對重癥急性胰腺炎腎損傷的保護作用[J].世界華人消化雜志, 2011, 19(32):3297-3301.
3. 張瑩, 石承先, 李玉祥, 等.丹參對重癥急性胰腺炎大鼠誘導型一氧化氮合成酶mRNA的表達與器官損傷的影響[J].中國中西醫結合雜志, 2005, 25(11):1012-1015.
4. 吳新正, 何迎春, 劉紅萍, 等.紅景天復方對小鼠免疫、耐缺氧和抗疲勞能力的影響[J].湖南中醫藥大學學報, 2008, 28(1):29-31.
5. 劉江正, 海春旭, 梁欣.紅景天醇提物對乙醇誘導QZG細胞氧化損傷的保護作用[J].癌變·畸變·突變, 2011, 23(2):81-86.
6. 查錫良, 周春燕.生物化學[M].第7版.北京:人民衛生出版社, 2008:371-374.
7. Kozhukhar AV, Yasinska IM, Sumbayev VV.Nitric oxide inhibits HIF-1alpha protein accumulation under hypoxic conditions:implication of 2-oxoglutarate and iron[J].Biochimie, 2006, 88(5):411-418.
8. Lieberthal W.Biology of ischemic and toxic renal tubular cell injury:role of nitric oxide and the inflammatory response[J].Curr Opin Nephrol Hypertens, 1998, 7(3):289-295.
9. 劉紅艷, 王海燕, 葉松, 等.姜黃素藥理作用及其機制研究進展[J].中國現代醫學雜志, 2012, 22(6):48-51.
10. 劉靜, 黃麗, 胡曉松, 等.姜黃素對大鼠重癥急性胰腺炎防治作用及其機制[J].中國普通外科雜志, 2012, 21(3):283-289.
11. 王莉, 趙樺.小叢紅景天脂溶性成分及抗菌活性分析[J].中國實驗方劑學雜志, 2012, 18(16):127-131.
12. Novovic S, Andersen AM, Ersb?ll AK, et al.Proinflammatory cytokines in alcohol or gallstone induced acute pancreatitis[J].JOP, 2009, 10(3):256-262.
13. 王先坤, 詹樂恒, 王世文, 等.IL-10、IL-18在重癥急性胰腺炎肺損傷中的作用[J].中國普外基礎與臨床雜志, 2005, 12(5):443-446.
14. 牛愛麗, 廖青, 張碩, 等.姜黃素對大強度運動大鼠腎損傷的保護作用及其機制[J].湖南師范大學學報:醫學版, 2012, 9(1):36-39.
15. Guan S, He J, Guo W, et al.Adjuvant effects of salidroside from Rhodiola rosea L.on the immune responses to ovalbumin in mice[J].Immunopharmacol Immunotoxicol, 2011, 33(4):738-743.
16. Lin SS, Chin LW, Chao PC, et al.In vivo Th1 and Th2 cytokine modulation effects of Rhodiola rosea standardised solution and its major constituent, salidroside[J].Phytother Res, 2011, 25(11):1604-1611.
17. Moore KW, de Waal Malefyt R, Coffman RL, et al.Interleukin-10 and the interleukin-10 receptor[J].Annu Rev Immunol, 2001, 19(2):683-765.
18. 李兆申, 張文俊, 李維勤, 等.胰腺炎[M].北京:科學出版社, 2010:94-95.
19. Selvam R, Subramanian L, Gayathri R, et al.The anti-oxidant activity of turmeric (Curcuma longa)[J].J Ethnopharmacol, 1995, 47(2):59-67.
20. 龔曉武, 李炳奇, 劉丹丹, 等.紅景天黃酮提取及其抗氧化活性研究[J].西北林學院學報, 2011, 26(3):136-138.
21. 許晗, 尹偉偉, 丁鼎.高山紅景天總甙抗衰老作用機理的實驗研究[J].浙江中醫藥大學學報, 2011, 35(2):248-249, 252.
22. 金艷玲, 周洋, 王洪海, 等.紅景天苷對大鼠腎臟缺血再灌注損傷的預防與保護作用[J].中藥新藥與臨床藥理, 2010, 21(1):22-25.
23. 馬天翔, 史寧, 陳乾, 等.紅景天中8種成分體外抗氧化作用的比較[J].中國藥理學通報, 2012, 28(9):1224-1228.
24. 張欣, 隋汝波.紅景天對大鼠缺血再灌注后細胞因子的影響[J].中國新藥雜志, 2008, 17(6):472-473, 485.
25. 惠遠見, 王衛星, 余佳, 等.急性胰腺炎大鼠腎臟中聚腺苷二磷酸核糖聚合酶-1 mRNA的表達及意義[J].中國普外基礎與臨床雜志, 2010, 17(3):272-274.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Protective and Therapeutic Effects of Curcumin Combined with Rhodiola on Rats with Severe Acute Pancreatitis Associated Renal Injury

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