Twist與E-cadherin在結直腸癌中的表達及其意義_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 錢江 , 韓佳 , 陳鵬 , 朱春麗 , 楊銀

重慶市墊江縣中醫院普外科（重慶墊江 408300）;

Corresponding?author：

錢江, Email: qianjiang688@126.com

Keywords：

結直腸癌; Twist蛋白; E-鈣粘素; 免疫組織化學

DOI：

10.7507/1007-9424.20150031

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

目的探討Twist和E-cadherin蛋白在結直腸癌組織中的表達及其可能的機理、相互作用以及與結直腸癌預后的關系。方法應用免疫組化SP法檢測63例結直腸癌組織以及21例正常結腸黏膜組織中Twist和E-cadherin蛋白的表達，并分析其與結直腸癌臨床病理特征和患者預后的關系。結果 Twist蛋白在結直腸癌組織中的表達陽性率為66.7%（42/63），而在正常結直腸黏膜組織中的表達陽性率為14.2%（3/21），前者高于后者（P＜0.01）；E-cadherin蛋白在結直腸癌組織中的表達陽性率為20.6%（13/63），而在正常結直腸黏膜組織中的表達陽性率為81.0%（17/21），前者低于后者（P＜0.01）。兩者的表達與腫瘤的分化程度及T、N和M分期均相關（P＜0.05）。相關性分析顯示，Twist和E-cadherin蛋白的表達呈負相關關系（r=-0.215，P=0.039）。結論 Twist和E-cadherin蛋白的表達可能與結直腸癌的發生發展和侵襲轉移相關，并可作為評價結直腸癌生物學行為和預后的重要指標。Twist和E-cadherin蛋白表達呈負相關關系提示，在上皮-間質轉化過程中可能是通過抑制E-cadherin蛋白的表達來實現的。

Citation： 錢江, 韓佳, 陳鵬, 朱春麗, 楊銀. Twist與E-cadherin在結直腸癌中的表達及其意義. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(1): 114-116. doi: 10.7507/1007-9424.20150031 Copy

1.	Thiery JP, Sleeman JP.Complex networks orchestrate epithelial-mesenchymal transitions.Nat Rev Mol Cell Biol, 2006, 7(2):131-142.
2.	Peinado H, Olmeda D, Cano A.Snail, Zeb and bHLH factors intumour progression:an alliance against the epithelial phenotype? NatRev Cancer, 2007, 7(6):415-428.
3.	Kyo S, Sakaguchi J, Ohno S, et al.High Twist expression is involvedin infiltrative endometrial cancer and affects patient survival.Hum Pathol, 2006, 37(4):431-438.
4.	Zhang Z1, Xie D, Li X, et al.Significance of TWIST expression and its association with E-cadherin in bladder cancer.Hum Pathol.2007, 38(4):598-606.
5.	Lee TK, Poon RT, Yuen AP, et al.Twist overexpression correlates with hepatocellular carcinoma metastasis through induction of epithelial-mesenchymal transition.Clin Cancer Res, 2006, 12(18):5369-5376.
6.	Yang Z, Zhang X, Gang H, et al.Up-regulation of gastric cancer cell invasion by Twist is accompanied by N-cadherin and fibronectin expression.Biochem Biophys Res Commun, 2007, 358(3):925-930.
7.	Early DS, Fontana L, Davidson NO.Translational approaches to addressing complex genetic pathways in colorectal cancer.Transl Res, 2008, 151(1):10-16.
8.	Lee S, Bang S, Song K, et al.Differential expression innormal adenoma carcinoma sequence suggests complex molecular carcinogenesis in colon.Oncol Rep, 2006, 16(4):747-754.
9.	Castanon I, Baylies MK.A Twist in fate:evolutionary comparison of TWIST structure and function.Gene, 2002, 287(1-2):11-22.
10.	Zuniga A, Quillet R, Perrin-Schmitt F, et al.Mouse Twist is required for fibroblast growth factor-mediated epithelial-mesenchymal signalling and cell survival during limb morphogenesis.Mech Dev, 2002, 114(1-2):51-59.
11.	Frackler MJ, McVeigh M, Evron E, et al.DNA methylation of RASSFIA, HIN-1, RAR-beta, Cyclin D2 and Twist in in situ and invasive lobular breast carcinoma.Int J Cancer, 2003, 107(6):970-975.
12.	Mehrota J, Vali M, McVeigh M et al.Very high frequency of hypermethylated genes in breast cancer metastasis to the bone, brain, and lung.Clin Cancer Res, 2004, 10(9):3104-3109.
13.	Yang MH, Wu MZ, Chiou SH, et al.Direct regulation of TWIST by HIF-1alpha promotes metastasis.Nat Cell Biol, 2008, 10(3):295-305.

1. Thiery JP, Sleeman JP.Complex networks orchestrate epithelial-mesenchymal transitions.Nat Rev Mol Cell Biol, 2006, 7(2):131-142.
2. Peinado H, Olmeda D, Cano A.Snail, Zeb and bHLH factors intumour progression:an alliance against the epithelial phenotype? NatRev Cancer, 2007, 7(6):415-428.
3. Kyo S, Sakaguchi J, Ohno S, et al.High Twist expression is involvedin infiltrative endometrial cancer and affects patient survival.Hum Pathol, 2006, 37(4):431-438.
4. Zhang Z1, Xie D, Li X, et al.Significance of TWIST expression and its association with E-cadherin in bladder cancer.Hum Pathol.2007, 38(4):598-606.
5. Lee TK, Poon RT, Yuen AP, et al.Twist overexpression correlates with hepatocellular carcinoma metastasis through induction of epithelial-mesenchymal transition.Clin Cancer Res, 2006, 12(18):5369-5376.
6. Yang Z, Zhang X, Gang H, et al.Up-regulation of gastric cancer cell invasion by Twist is accompanied by N-cadherin and fibronectin expression.Biochem Biophys Res Commun, 2007, 358(3):925-930.
7. Early DS, Fontana L, Davidson NO.Translational approaches to addressing complex genetic pathways in colorectal cancer.Transl Res, 2008, 151(1):10-16.
8. Lee S, Bang S, Song K, et al.Differential expression innormal adenoma carcinoma sequence suggests complex molecular carcinogenesis in colon.Oncol Rep, 2006, 16(4):747-754.
9. Castanon I, Baylies MK.A Twist in fate:evolutionary comparison of TWIST structure and function.Gene, 2002, 287(1-2):11-22.
10. Zuniga A, Quillet R, Perrin-Schmitt F, et al.Mouse Twist is required for fibroblast growth factor-mediated epithelial-mesenchymal signalling and cell survival during limb morphogenesis.Mech Dev, 2002, 114(1-2):51-59.
11. Frackler MJ, McVeigh M, Evron E, et al.DNA methylation of RASSFIA, HIN-1, RAR-beta, Cyclin D2 and Twist in in situ and invasive lobular breast carcinoma.Int J Cancer, 2003, 107(6):970-975.
12. Mehrota J, Vali M, McVeigh M et al.Very high frequency of hypermethylated genes in breast cancer metastasis to the bone, brain, and lung.Clin Cancer Res, 2004, 10(9):3104-3109.
13. Yang MH, Wu MZ, Chiou SH, et al.Direct regulation of TWIST by HIF-1alpha promotes metastasis.Nat Cell Biol, 2008, 10(3):295-305.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Twist與E-cadherin在結直腸癌中的表達及其意義

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content