Proliferation and Apoptosis of Karpas299 Cells Induced by Adenovirus-Mediated Interleukin-24 Gene_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 JIANGGuang-bin ¹ ,  LIULei ² , SUNWan-bang ¹ , YANGJi-cheng ³ , GUOJin-jin ¹

1. Graduate School of Zhuhai Campus, Zunyi Medical College, Zhuhai 519041, Guangdong Province, China;
2. Department of General Surgery, Shen Zhen Children's Hospital, Shenzhen 518038, Guangdong Province, China;
3. Department of Immunology, Suzhou University, Suzhou 215021, Jiangsu Province, China;

Corresponding?author：

LIULei, Email: liulei3322@aliyun.com

Keywords：

Adenovirus-mediated interleukin-24 gene; Anaplastic large-cell lymphoma; Gene therapy

DOI：

10.7507/1007-9424.20150077

Video：

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Objective To investigate the proliferation and apoptosis effects of adenovirus-mediated interleukin-24 (Ad-IL-24) gene on Karpas299 cells in vitro. Methods The Karpas299 cells were divided into blank control group, Ad-IL-24 group, and the adenovirus which carrying green fluorescent protein gene group (Ad-GFP group). Karpas299 cells of Ad-IL-24 group were infected by adding 200.0 μL Ad-IL-24, Karpas299 cells of Ad-GFP group were infected by adding 200.0 μL Ad-GFP, but Karpas299 cells of blank control group were treated by adding 200.0 μL PBS. Cells' proliferation inhibition rates of 3 groups were detected by cell counting kit (CCK-8) method at 12, 24, and 48 hours after treatment, respectively, and the cells' apoptosis rates of 3 groups were detected by flow cytometry at 48 hours after treatment. Results Ad-IL-24 can suppress the growth of Karpas299 cells, and the inhibition rate increased over time. Compared with Ad-GFP group at the same time, the cell' proliferation inhibition rate of Ad-IL-24 group was higher at 12, 24, and 48 hours after treatment (P<0.05). In addition, the cells' apoptosis rate of Ad-IL-24 group was higher than those of Ad-GFP group and blank control group at 48 hours after treatment (P<0.05). Conclusion Ad-IL-24 can suppress the growth of Karpas299 cells and induce the apoptosis of it.

Citation： JIANGGuang-bin, LIULei, SUNWan-bang, YANGJi-cheng, GUOJin-jin. Proliferation and Apoptosis of Karpas299 Cells Induced by Adenovirus-Mediated Interleukin-24 Gene. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(3): 284-288. doi: 10.7507/1007-9424.20150077 Copy

1.	?藍必全, 侯梅. 間變性大細胞淋巴瘤治療的研究進展. 華西醫學, 2006, 21(3):623-624.
2.	Le Deley M, Rosolen A, Williams DM, et al. Vinblastine in children andadolescents with high-risk anaplastic large-cell lymphoma:results of the randomized ALCL99-vinblastine trial. J Clin Oncol, 2010, 28(25):3987-3993.
3.	苗慧, 陸鳳娟, 翟曉文, 等. 兒童間變型大細胞性非霍奇金淋巴瘤8例臨床特點及治療. 中國小兒血液與腫瘤雜志, 2008, 13(4):161-165.
4.	Dent P, Yacoub A, Hamed HA, et al. MDA-7/IL-24 as a cancer therapeutic:from bench to bedside. Anticancer Drugs, 2010, 21(8):725-731.
5.	汪小華, 葉震敏, 盛偉華, 等. 腺病毒介導hIL-24抑制裸鼠肝癌荷瘤生長及其機制研究. 生物工程學報, 2006, 22(6):925-930.
6.	王晉芬, 王云中, 陳振文, 等. 根據WHO淋巴造血系統腫瘤新分類對山西省淋巴瘤分布特點的分析. 中華病理學雜志, 2006, 35(4):218-223.
7.	皋嵐湘, 丁華野, 趙坡, 等. 軟組織及骨的小細胞型間變性大細胞淋巴瘤1例報道. 臨床與實驗病理學雜志, 2000, 16(2):107-110.
8.	胡亞, 杜瀟, 廖泉, 等. 原發性胰腺淋巴瘤的診療分析. 中國普外基礎與臨床雜志, 2008, 15(4): 285-286.
9.	Seidemann K, Tiemann M, Schrappe M, et al. Short-pulse B-non-Hodgkin lymphoma-type chemotherapy is efficacious treatment for pediatric anaplastic large cell lymphoma:a report of the Berlin-Frankfurt-Münster Group Trial NHL-BFM 90. Blood, 2001, 97(12):3699-3706.
10.	鄭卓軍, 湯永民. 惡性血液腫瘤患兒化療后感染產ESBLs細菌耐藥性分析. 中國當代兒科雜志, 2012, 14(7):518-520.
11.	曠凌寒, 江詠梅, 胡正強, 等. 兒科血液病房血培養病原菌分布及耐藥情況分析. 中國當代兒科雜志, 2013, 15(4):259-263.
12.	葉啟東, 湯靜燕, 潘慈, 等. 兒童間變性大細胞淋巴瘤20例臨床療效分析. 中國小兒血液與腫瘤雜志, 2012, 17(1):13-16.
13.	Jiang H, Lin JJ, Su ZZ, et al. Subtraction hybridization identifies a novel melanoma differentiation associated gene, mda-7, modulated during human melanoma differentiation, growth and progression. Oncogene, 1995, 11(12):2477-2486.
14.	王少慧, 鄭銳青, 孫萬邦. mda-7/IL-24及其抗腫瘤機理研究進展. 國際免疫學雜志, 2013, 36(1):40-44.
15.	Caudell EG, Mumm JB, Poindexter N, et al. The protein product of the tumor suppressor gene, melanoma differentiation-associated gene 7, exhibits immunostimulatory activity and is designatedIL-24. J Immunol, 2002, 168(12):6041-6046.
16.	Park MA, Walker T, Martin AP, et al. MDA-7/IL-24-induced cell killing in malignant renal carcinoma cells occurs by a ceramide/CD95/PERK-dependent mechanism. Mol Cancer Ther, 2009, 8(5):1280-1291.
17.	Huang EY, Madireddi MT, Gopalkrishnan RV, et al. Genomic structure, chromosomal localization and expression profile of a novel melanoma differentiation associated (mda-7) gene with cancer specific growth suppressing and apoptosis inducing properties. Oncogene, 2001, 20(48):7051-7063.
18.	王從俊, 彭志海, 鄭建偉, 等. 攜帶mda-7基因的復制缺陷型腺病毒通過促進線粒體釋放促凋亡蛋白殺傷肝癌細胞. 中華腫瘤雜志, 2008, 30(9):649-651.
19.	Emdad L, Lebedeva IV, Su ZZ, et al. Melanoma differentiation associated gene-7/interleukin-24 reverses multidrug resistance in human colorectal cancer cells. Mol Cancer Ther, 2007, 6(11):2985-2994.
20.	Dash R, Dmitriev I, Su ZZ, et al. Enhanced delivery of mda-7/IL-24 using a serotype chimeric adenovirus (Ad.5/3) improves therapeutic efficacy in low CAR prostate cancer cells. Cancer Gene Ther, 2010, 17(7):447-456.
21.	王少慧, 郭錦錦, 唐艷麗, 等. 重組人白細胞介素-24及其聯合順鉑對卵巢癌細胞體外生長的影響. 細胞與分子免疫學雜志, 2014, 30(1):33-35.
22.	郭錦錦, 王少慧, 孫萬邦, 等. 重組人IL-24對人肺腺癌A549/DDP細胞增殖活性的影響. 中國免疫學雜志, 2014, 30(9):1178-1181.
23.	郭錦錦, 王少慧, 孫萬邦, 等. 重組人IL-24聯合順鉑體外抗肺癌細胞生長的實驗研究. 免疫學雜志, 2014, 30(9):778-781.
24.	Jia J, Li S, Gong W, et al. mda-7/IL-24 induces apoptosis in human GBC-SD gallbladder carcinoma cells via mitochondrial apoptotic pathway. Oncol Rep, 2011, 25(1):195-201.
25.	王少慧, 郭錦錦, 唐艷麗, 等. 多基因遺傳表達分析系統檢測rhIL-24誘導人卵巢癌細胞凋亡相關基因的應用. 中國免疫學雜志, 2013, 29(12):1312-1316.
26.	Tamai H, Miyake K, Yamaguchi H, et al. AAV8 vector expressing IL24 efficiently suppresses tumor growth mediated by specific mechanisms in MLL/AF4-positive ALL model mice. Blood, 2012, 119(1):64-71.

1. ?藍必全, 侯梅. 間變性大細胞淋巴瘤治療的研究進展. 華西醫學, 2006, 21(3):623-624.
2. Le Deley M, Rosolen A, Williams DM, et al. Vinblastine in children andadolescents with high-risk anaplastic large-cell lymphoma:results of the randomized ALCL99-vinblastine trial. J Clin Oncol, 2010, 28(25):3987-3993.
3. 苗慧, 陸鳳娟, 翟曉文, 等. 兒童間變型大細胞性非霍奇金淋巴瘤8例臨床特點及治療. 中國小兒血液與腫瘤雜志, 2008, 13(4):161-165.
4. Dent P, Yacoub A, Hamed HA, et al. MDA-7/IL-24 as a cancer therapeutic:from bench to bedside. Anticancer Drugs, 2010, 21(8):725-731.
5. 汪小華, 葉震敏, 盛偉華, 等. 腺病毒介導hIL-24抑制裸鼠肝癌荷瘤生長及其機制研究. 生物工程學報, 2006, 22(6):925-930.
6. 王晉芬, 王云中, 陳振文, 等. 根據WHO淋巴造血系統腫瘤新分類對山西省淋巴瘤分布特點的分析. 中華病理學雜志, 2006, 35(4):218-223.
7. 皋嵐湘, 丁華野, 趙坡, 等. 軟組織及骨的小細胞型間變性大細胞淋巴瘤1例報道. 臨床與實驗病理學雜志, 2000, 16(2):107-110.
8. 胡亞, 杜瀟, 廖泉, 等. 原發性胰腺淋巴瘤的診療分析. 中國普外基礎與臨床雜志, 2008, 15(4): 285-286.
9. Seidemann K, Tiemann M, Schrappe M, et al. Short-pulse B-non-Hodgkin lymphoma-type chemotherapy is efficacious treatment for pediatric anaplastic large cell lymphoma:a report of the Berlin-Frankfurt-Münster Group Trial NHL-BFM 90. Blood, 2001, 97(12):3699-3706.
10. 鄭卓軍, 湯永民. 惡性血液腫瘤患兒化療后感染產ESBLs細菌耐藥性分析. 中國當代兒科雜志, 2012, 14(7):518-520.
11. 曠凌寒, 江詠梅, 胡正強, 等. 兒科血液病房血培養病原菌分布及耐藥情況分析. 中國當代兒科雜志, 2013, 15(4):259-263.
12. 葉啟東, 湯靜燕, 潘慈, 等. 兒童間變性大細胞淋巴瘤20例臨床療效分析. 中國小兒血液與腫瘤雜志, 2012, 17(1):13-16.
13. Jiang H, Lin JJ, Su ZZ, et al. Subtraction hybridization identifies a novel melanoma differentiation associated gene, mda-7, modulated during human melanoma differentiation, growth and progression. Oncogene, 1995, 11(12):2477-2486.
14. 王少慧, 鄭銳青, 孫萬邦. mda-7/IL-24及其抗腫瘤機理研究進展. 國際免疫學雜志, 2013, 36(1):40-44.
15. Caudell EG, Mumm JB, Poindexter N, et al. The protein product of the tumor suppressor gene, melanoma differentiation-associated gene 7, exhibits immunostimulatory activity and is designatedIL-24. J Immunol, 2002, 168(12):6041-6046.
16. Park MA, Walker T, Martin AP, et al. MDA-7/IL-24-induced cell killing in malignant renal carcinoma cells occurs by a ceramide/CD95/PERK-dependent mechanism. Mol Cancer Ther, 2009, 8(5):1280-1291.
17. Huang EY, Madireddi MT, Gopalkrishnan RV, et al. Genomic structure, chromosomal localization and expression profile of a novel melanoma differentiation associated (mda-7) gene with cancer specific growth suppressing and apoptosis inducing properties. Oncogene, 2001, 20(48):7051-7063.
18. 王從俊, 彭志海, 鄭建偉, 等. 攜帶mda-7基因的復制缺陷型腺病毒通過促進線粒體釋放促凋亡蛋白殺傷肝癌細胞. 中華腫瘤雜志, 2008, 30(9):649-651.
19. Emdad L, Lebedeva IV, Su ZZ, et al. Melanoma differentiation associated gene-7/interleukin-24 reverses multidrug resistance in human colorectal cancer cells. Mol Cancer Ther, 2007, 6(11):2985-2994.
20. Dash R, Dmitriev I, Su ZZ, et al. Enhanced delivery of mda-7/IL-24 using a serotype chimeric adenovirus (Ad.5/3) improves therapeutic efficacy in low CAR prostate cancer cells. Cancer Gene Ther, 2010, 17(7):447-456.
21. 王少慧, 郭錦錦, 唐艷麗, 等. 重組人白細胞介素-24及其聯合順鉑對卵巢癌細胞體外生長的影響. 細胞與分子免疫學雜志, 2014, 30(1):33-35.
22. 郭錦錦, 王少慧, 孫萬邦, 等. 重組人IL-24對人肺腺癌A549/DDP細胞增殖活性的影響. 中國免疫學雜志, 2014, 30(9):1178-1181.
23. 郭錦錦, 王少慧, 孫萬邦, 等. 重組人IL-24聯合順鉑體外抗肺癌細胞生長的實驗研究. 免疫學雜志, 2014, 30(9):778-781.
24. Jia J, Li S, Gong W, et al. mda-7/IL-24 induces apoptosis in human GBC-SD gallbladder carcinoma cells via mitochondrial apoptotic pathway. Oncol Rep, 2011, 25(1):195-201.
25. 王少慧, 郭錦錦, 唐艷麗, 等. 多基因遺傳表達分析系統檢測rhIL-24誘導人卵巢癌細胞凋亡相關基因的應用. 中國免疫學雜志, 2013, 29(12):1312-1316.
26. Tamai H, Miyake K, Yamaguchi H, et al. AAV8 vector expressing IL24 efficiently suppresses tumor growth mediated by specific mechanisms in MLL/AF4-positive ALL model mice. Blood, 2012, 119(1):64-71.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Proliferation and Apoptosis of Karpas299 Cells Induced by Adenovirus-Mediated Interleukin-24 Gene

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