Study The Expressions of FN and PTEN in Hepatocellular Carcinoma and Its Clinical Significance_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 WUJin-zhu ,  CAIWei-hua , GUChun-yan , LURen-fei , WUJian-jun , XIAOXu , WANGJian-xin , ZHURen-fei

Department of Hepatobiliary Surgery, The Third Affiliated Hospital, Nantong University, Nantong 226010, Jiangsu Province, China;

Corresponding?author：

CAIWei-hua, Email: 39235978@qq.com

Keywords：

Fibronectin; Phosphatase and tensin homology deleted on chromosome ten; Hepatocellular carcinoma; Gene expression

DOI：

10.7507/1007-9424.20150212

Video：

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Objective To explore the relationship between the expressions of fibronectin (FN) and phosphatase and tensin homology deleted on ehromosome ten (PTEN) in hepatocellular carcinoma (HCC) tissues and the clinical pathological features. Methods The expressions of FN and PTEN were detected by using Western blot and immunohistochemistry respectively in 83 HCC tissues and para-carcinoma tissues, 47 hepatic cirrhosis tissues and 11 normal hepatic tissues. The correlations between the expressions of FN and PTEN and the clinicopathologic features of HCC patients were analyzed. Results The positive expression rate of FN protein in HCC tissues was significantly higher than those in para-carcinoma tissue, normal hepatic tissue, and liver cirrhosis tissues (P<0.05); meanwhile the expression of PTEN was opposite (P<0.05). The positive expression rate of FN protein in para-carcinoma tissues was also obviously higher than that liver cirrhosis tissues and normal hepatic tissues (P<0.05), meanwhile the expression of PTEN was opposite (P<0.05). The positive expression rate of FN protein was higher in HCC tissues with cancer embolus, lymphatic metastasis, positive AFP, and multiple tumor (P<0.05), but there were no statistically significant differences in FN protein expression, gender, age, HBsAg, degree of tumor differentiation, and size of tumor (P>0.05). The positive expression rate of PTEN was lower in HCC tissues with high-medium differentiation, cancer embolus, positive AFP, lymphatic metastasis, and tumor diameter ≥2 cm (P<0.05), there were no statistically significant differences in PTEN expression, gender, age, HBsAg, and the number of tumor (P>0.05). Conclusions The abnormal expressions of FN and PTEN in HCC tissues which may play a role in promoting or inhibiting occurrence, development, invasion, and metastasis of HCC. The abnormal expressions of both can be used as molecular biological markers for the malignant degree, invasion, and metastasis of HCC.

Citation： WUJin-zhu, CAIWei-hua, GUChun-yan, LURen-fei, WUJian-jun, XIAOXu, WANGJian-xin, ZHURen-fei. Study The Expressions of FN and PTEN in Hepatocellular Carcinoma and Its Clinical Significance. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2015, 22(7): 822-827. doi: 10.7507/1007-9424.20150212 Copy

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2.	Pollicino T, Saitta C, Raimondo G. Hepatocellular carcinoma:the point of view of the hepatitis B virus. Carcinogenesis, 2011, 32(8):1122-1132.
3.	康凱夫, 張鑫, 陳小伍, 等. MMP-7和PTEN蛋白在原發性肝細胞性肝癌中的表達及其意義. 腫瘤防治研究, 2010, 37(6):652-655.
4.	劉梅, 楊勝利, 楊付葉, 等. 一個新的與纖粘連蛋白相互作用促進肝癌細胞遷移的基因. 中國腫瘤臨床, 2006, 33 (14):781-783.
5.	Obara M, Sakuma T, Fujikawa K. The third type Ⅲ module of human fibroectin mediates cell adhesion and migration. J Biochem, 2010, 147(3):327-335.
6.	Antonyak MA, Li B, Boroughs JL, et al. Cancer cell-derived microvesicles induce transformation by transferring tissue tran sglutaminase and fibronectin to recipient cues. Proc Natl Acad Sci USA, 2011, 108(12):4852-4857.
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8.	秦權林. PTEN基因多態性及PTEN蛋白表達與家族聚集性肝癌遺傳易感性研究. 南寧:廣西醫科大學, 2009.
9.	Liu H, Huang X, Liu X, et al. miR-21 promotes human nucleus pulposus cell proliferation through PTEN/AKT signaling. Int J Mol Sci, 2014, 15(3):4007-4018.
10.	Athnassiadou P, Athanassiadesy P, Grapsa D, et al. The prognostic value of PTEN, p53, and beta-catenin in endometrial carcinoma:a prospective immunocytochemical study. Int J Gynecol Cancer, 2007, 17(3):697-704.
11.	Kechagioglou P, Papi RM, Provatopoulou X, et al. Tumor suppressor PTEN in breast cancer:heterozygosity, mutations and protein expression. Anticancer Res, 2014, 34(3):1387-1400.
12.	Stanich PP, Pilarski R, Rock J, et al. Colonic manifestations of PTEN hamartoma tumor syndrome:case series and systematic review. World J Gastroenterol, 2014, 20(7):1833-1838.
13.	Nogueira C, Kim KH, Sung H, et al. Cooperative interactions of PTEN deficiency and RAS activation in melanoma metastasis. Oncogene, 2010, 29(47):6222-6232.
14.	張纓, 賈紹昌, 項方, 等. miR-21在肝癌細胞中的表達及其與抑癌基因PTEN的關系. 臨床腫瘤學雜志, 2014, 19(6):490-493.
15.	Zhang M, Shan BE, Yuan NF, et al. Effect of topotecan on retinocytoma cell apoptosis and expression of Livin and PTEN. Chin Med J (Engl), 2013, 126(2):340-344.
16.	白麗, 任鋒, 鄭素軍, 等. 抑制PTEN對小鼠肝缺血再灌注損傷的保護作用及其機制. 中華肝臟病雜志, 2014, 22(6):451-455.
17.	郝禮森, 張曉嵐, 任昌鎮, 等. PTEN過表達抑制體外活化肝星狀細胞Akt的磷酸化. 實用醫學雜志, 2014, 30(7):1069-1072.
18.	張蕾, 楊芳, 張曉敏, 等. HBsAg陽性肝癌PTEN基因表達的研究. 濱州醫學院學報. 2014, 37(4):241-243.
19.	張鳴杰, 張國雷, 魏云海, 等. FHIT、FN和PTEN在肝癌組織中的表達及臨床意義. 中華普通外科雜志, 2012, 27(6):487-490.
20.	李秋陽, 陳惠麗, 李寧, 等. MMP-9和PTEN蛋白在膠質瘤中的表達特點及意義. 中國實驗診斷學, 2013, 17(10):1811-1814.
21.	Chen F, Yang D, Wang S, et al. Livin regulates prostate cancer cell invasion by impacting the NF-kβsignaling pathway and the expression of FN and CXCR4. IUBMB Life, 2012, 64(3):274-283.
22.	Zhang Z, Pan J, Li L, et al. Survey of risk factors contributed to lymphatic metastasis in patients with oral tongue squamous cell carcinoma by immunohistochemistry. J Oral Pathol Med, 2011, 40(2):127-134.
23.	黃宗明, 余英豪, 于媛媛, 等. 胃癌組織中CD44v6、VEGF、PTEN的表達與臨床意義. 臨床誤診誤治雜志, 2012, 25(3):39-42.
24.	王鵬, 郭琳, 王強. PTEN、Fas/FasL和MMP-2在胃癌組織的表達及其臨床意義. 中國普外基礎與臨床雜志, 2009, 16(12):991-995.
25.	叢玲華, 高志安, 鐘國平, 等. 人肺癌組織E-cadherin和PTEN及HIF-1α蛋白表達臨床意義的探討. 中華腫瘤防治雜志, 2013, 20(3):196-199.
26.	鐘育波, 孫凱, 張曉檳, 等. X線輻射劑量對結直腸癌細胞microRNA-221和PTEN表達的影響. 中國普外基礎與臨床雜志, 2013, 20(10):1113-1116.

1. ?Silveira AP, Da Silva Manoel-Caetano F, Aoki S, et al. Gene mutations and polymorphisms of TP53 and FHIT in chronic esophagitis and esophageal carcinoma. Anticancer Res, 2011, 31(5):1685-1690.
2. Pollicino T, Saitta C, Raimondo G. Hepatocellular carcinoma:the point of view of the hepatitis B virus. Carcinogenesis, 2011, 32(8):1122-1132.
3. 康凱夫, 張鑫, 陳小伍, 等. MMP-7和PTEN蛋白在原發性肝細胞性肝癌中的表達及其意義. 腫瘤防治研究, 2010, 37(6):652-655.
4. 劉梅, 楊勝利, 楊付葉, 等. 一個新的與纖粘連蛋白相互作用促進肝癌細胞遷移的基因. 中國腫瘤臨床, 2006, 33 (14):781-783.
5. Obara M, Sakuma T, Fujikawa K. The third type Ⅲ module of human fibroectin mediates cell adhesion and migration. J Biochem, 2010, 147(3):327-335.
6. Antonyak MA, Li B, Boroughs JL, et al. Cancer cell-derived microvesicles induce transformation by transferring tissue tran sglutaminase and fibronectin to recipient cues. Proc Natl Acad Sci USA, 2011, 108(12):4852-4857.
7. 王麗. 抑癌基因PTEN在肝癌組織中的表達及HCV核心蛋白對PTEN作用的研. 西安:第四軍醫大學, 2007.
8. 秦權林. PTEN基因多態性及PTEN蛋白表達與家族聚集性肝癌遺傳易感性研究. 南寧:廣西醫科大學, 2009.
9. Liu H, Huang X, Liu X, et al. miR-21 promotes human nucleus pulposus cell proliferation through PTEN/AKT signaling. Int J Mol Sci, 2014, 15(3):4007-4018.
10. Athnassiadou P, Athanassiadesy P, Grapsa D, et al. The prognostic value of PTEN, p53, and beta-catenin in endometrial carcinoma:a prospective immunocytochemical study. Int J Gynecol Cancer, 2007, 17(3):697-704.
11. Kechagioglou P, Papi RM, Provatopoulou X, et al. Tumor suppressor PTEN in breast cancer:heterozygosity, mutations and protein expression. Anticancer Res, 2014, 34(3):1387-1400.
12. Stanich PP, Pilarski R, Rock J, et al. Colonic manifestations of PTEN hamartoma tumor syndrome:case series and systematic review. World J Gastroenterol, 2014, 20(7):1833-1838.
13. Nogueira C, Kim KH, Sung H, et al. Cooperative interactions of PTEN deficiency and RAS activation in melanoma metastasis. Oncogene, 2010, 29(47):6222-6232.
14. 張纓, 賈紹昌, 項方, 等. miR-21在肝癌細胞中的表達及其與抑癌基因PTEN的關系. 臨床腫瘤學雜志, 2014, 19(6):490-493.
15. Zhang M, Shan BE, Yuan NF, et al. Effect of topotecan on retinocytoma cell apoptosis and expression of Livin and PTEN. Chin Med J (Engl), 2013, 126(2):340-344.
16. 白麗, 任鋒, 鄭素軍, 等. 抑制PTEN對小鼠肝缺血再灌注損傷的保護作用及其機制. 中華肝臟病雜志, 2014, 22(6):451-455.
17. 郝禮森, 張曉嵐, 任昌鎮, 等. PTEN過表達抑制體外活化肝星狀細胞Akt的磷酸化. 實用醫學雜志, 2014, 30(7):1069-1072.
18. 張蕾, 楊芳, 張曉敏, 等. HBsAg陽性肝癌PTEN基因表達的研究. 濱州醫學院學報. 2014, 37(4):241-243.
19. 張鳴杰, 張國雷, 魏云海, 等. FHIT、FN和PTEN在肝癌組織中的表達及臨床意義. 中華普通外科雜志, 2012, 27(6):487-490.
20. 李秋陽, 陳惠麗, 李寧, 等. MMP-9和PTEN蛋白在膠質瘤中的表達特點及意義. 中國實驗診斷學, 2013, 17(10):1811-1814.
21. Chen F, Yang D, Wang S, et al. Livin regulates prostate cancer cell invasion by impacting the NF-kβsignaling pathway and the expression of FN and CXCR4. IUBMB Life, 2012, 64(3):274-283.
22. Zhang Z, Pan J, Li L, et al. Survey of risk factors contributed to lymphatic metastasis in patients with oral tongue squamous cell carcinoma by immunohistochemistry. J Oral Pathol Med, 2011, 40(2):127-134.
23. 黃宗明, 余英豪, 于媛媛, 等. 胃癌組織中CD44v6、VEGF、PTEN的表達與臨床意義. 臨床誤診誤治雜志, 2012, 25(3):39-42.
24. 王鵬, 郭琳, 王強. PTEN、Fas/FasL和MMP-2在胃癌組織的表達及其臨床意義. 中國普外基礎與臨床雜志, 2009, 16(12):991-995.
25. 叢玲華, 高志安, 鐘國平, 等. 人肺癌組織E-cadherin和PTEN及HIF-1α蛋白表達臨床意義的探討. 中華腫瘤防治雜志, 2013, 20(3):196-199.
26. 鐘育波, 孫凱, 張曉檳, 等. X線輻射劑量對結直腸癌細胞microRNA-221和PTEN表達的影響. 中國普外基礎與臨床雜志, 2013, 20(10):1113-1116.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study The Expressions of FN and PTEN in Hepatocellular Carcinoma and Its Clinical Significance

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