Protective Effect of Low-Molecular-Weight Heparin Calcium Combined with Trimetazi-dine on Intestinal Smooth Muscle of Intestinal Acute Mesangial Vein Thrombosis in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 TUCan-neng ,  CHENJian-li , CHENJun-mao , WANGChang-you , WANGXiao-tao , ZHANGGuo-zhi

Department of General Surgery, The Affiliated Hospital, North China University of Science and Technology, Tangshan 063000, Hebei Province, China;

Corresponding?author：

CHENJian-li, Email: mychenjianli@126.com

Keywords：

Acute mesenteric venous thrombosis; Intestinal smooth muscle; Low molecular weight heparin calcium; Trimetazidine

DOI：

10.7507/1007-9424.20160111

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To explore the protective effect of low-molecular-weight heparin calcium (LHC) combined with trimetazidine on intestinal smooth muscle of intestinal acute mesangial vein thrombosis (AMVT) in rats and it's mechanism of effect. Methods A total of 120 SD male rats were randomly divided into three groups, with 40 rats in each group. LHC group: after the AMVT model established, rats were subcutaneous injection the LHC (30 U/100 g) per 12 h until 72 h after surgery. LHC+trimetazidine group (LHCT group): after the AMVT model established, rats were subcutaneous injection the LHC (30 U/100 g) and tail vein injection the trimetazidine (10 mg/kg) per 12 h until 72 h after surgery. Normal saline group (NS group): after the AMVT model established, rats were subcutaneous injection the NS (0.2 mL/100 g) per 12 h until 72 after surgery. The AMVT model were established by blocking superior mesenteric vein of 8 cm and the edge vein arch. Vena cava blood samples and intestinal segments were collected sequentially at 6 h, 12 h, 24 h, 48 h and 72 h afrer surgery. The levels of malondialdehyde (MDA) and creatine kinase (CK) in the blood, and the level of ATP in the intestinal tissue samples were measured with ELISA. Intestinal tissue were taken from the rats for inestinal tissue section, stained with hematoxylin and eosin, examined under light microscopy and evaluated histopathologically using mesemeche scoring system at different time. Results Compared with the LHC group and NS group, the levels of MDA and CK in blood and histopathology score of intestinal tissues in rats were significantly decreased, and the level of ATP significantly increased in LHCT group at different time point (P < 0.05). Conclusion Trimetazidine can improve intestinal smooth muscle energy metabolism in the AMVT disease, comined with LHC early can avoid intestinal smooth muscle wall permeability coagulation necrosis and reduce the intestinal smooth muscle damage.

Citation： TUCan-neng, CHENJian-li, CHENJun-mao, WANGChang-you, WANGXiao-tao, ZHANGGuo-zhi. Protective Effect of Low-Molecular-Weight Heparin Calcium Combined with Trimetazi-dine on Intestinal Smooth Muscle of Intestinal Acute Mesangial Vein Thrombosis in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(4): 416-420. doi: 10.7507/1007-9424.20160111 Copy

1.	陳建立, 劉彤, 戚峰.腸系膜上靜脈血栓形成的實驗及臨床研究.天津醫科大學, 2012, 81-82.
2.	Musemeche CA. A model of instinal ischemia in the neonatal rat utiling superior mesenteric artery occlusion and intraluminal plateletactivating factor. J surg Res, 1995, 58(6): 724-727.
3.	Hmoud B, Singal AK, Kamath PS. Mesenteric venous thrombosis. Clin Exp Hepatol, 2014, 4(3): 257-263.
4.	何文梅, 郭虹霞.急性腸系膜靜脈血栓形成8例診治分析.現代診斷與治療, 2014, 25(22): 515-514.
5.	戈小虎, 朱峰.急性腸系膜靜脈血栓形成的診治進展.中國血管外科雜志:電子版, 2015, 7(4): 217-219.
6.	Acosta S, Krantz P. Trends in prevalence of fatal surgical diseases at forensic autopsy. ANZ J Surg, 2007, 77(9): 718-721.
7.	Condat B, Pessione F, Helene-Denninger M, et al. Recent portal or mesenteric venous thmmbosis:increased recognmon and fhquent recanalization on anticoagulant therapy. Hepatology, 2000, 32(3): 466-470．.
8.	許慶, 鄧淼, 張栩, 等.急性腸系膜靜脈血栓形成的臨床診治策略(附19例報告).臨床和實驗醫學雜志, 2014, 13(13): 1099-1101.
9.	陳軍, 夏培元, 常山, 等.燒傷血清對腸上皮細胞屏障功能損傷的實驗研究.中華創傷雜志, 2002, 18(5):301-304.
10.	Krendel MF, Bonder EM. Analysis of actin filament bundle dynamics during contact formation in live epithelial cells. Cell Motil Cytoskeleton, 1999, 43(4): 296-309.
11.	張永珍, 楊立新, 閻西艴, 等.比索洛爾長程口服療法改善冠心病病人的左室舒張功能不全.新藥與臨床, 1997, 16(5): 273-275.
12.	黃震華, 徐濟民.尼卡地平治療心絞痛.新藥與臨床, 1994, 13(3): 169-172.
13.	劉宏鳴, 樊志勝, 曹魯魯.地爾硫靜脈滴注治療冠心病優于丹參注射液.中國新藥與臨床雜志, 1998, 17(6): 355-357.
14.	Veitch K, maisin L, Hue L. Trimetazidine ef-fects on the Damage to Mitochondrial Function caused by ischemia and Reperfusion. Am J Cardiol, 1995, 76(6): 25B-30B.
15.	Broderick TL, Quinney HA, Barker CC, et al. Beneficial effect of carnitine on mechanical recovery of rat hearts reperfused after a transient period of global ischemia is accompanied by a stimulation of glucose oxidation. Circulation, 1993, 87(3): 972-981.
16.	Morin D, Elimadi A, Sapena R, et al. Evidence for the existence of [3H]-trimetazidine binding sites involved in the regulation of the mitochondrial permeability transition pore. Br J Pharmacol, 1998, 123(7): 1385-1394.
17.	Tselepis A, Doulias P, htrida E, et al. Tirmetazidine protects lowden sity lipoproteins from oxidation and cultured cells exposed to H₂O₂ from DNA damge. Free Radic Biol Med, 2001, 30(12): 1357-1364.
18.	Noble MI, belcheer PR. Drake-Holland AJ. Limitation of infarct size by trimetazidine in the rabbit. Am J Cardiol, 1995, 76(6):41B-44B.
19.	Demasion L, Fantini E, Sentex E, et al. Trimetazidine: in vitro influence on heart mitochodrial function. Am J Cardiol, 1995, 96(6): 31B-37B.
20.	Croall DE, DeMartino GN. Calcium-activated neutral protease (calpain) system: structure, function, and regulation. Physiol Rev, 1991, 71(3): 813-847.
21.	Beynon RJ, Bond JS. Catabolism of intracellular protein: molecular aspects. Am J Physiol, 1986, 251(2 Pt 1): C141-C152.
22.	Topham PS, Haydar SA, Kuphal R, et al. Complement-mediated injury reversibly disrupts glomerular epithelial cell actin microfila ments and focaladhesions. Kidney Int, 1999, 55(5): 1763-1775.
23.	顧忠平.曲美他嗪治療缺血性心力衰竭的臨床療效觀察.泰山醫學院學報, 2014, 35(8): 798-799.
24.	楊明.曲美他嗪治療勞累性心絞痛的療效觀察.中西醫結合心血管病雜志, 2015, 3(7): 70-71.
25.	王健, 黃元偉, 魏經漢, 等.曲美他嗪對家兔心肌缺血再灌注損傷的保護作用.浙江大學校報, 2003, 32(3): 219-222.
26.	李選, 歐陽強, 蕭湘生.腸系膜靜脈血栓的介入治療.介入放射學雜志, 2006, 15(4): 202-205.
27.	Rhee Ry, Gloviczki P, Mesenteric venous thrombosis. Surg Clin North am, 1997, 77(2): 327-338.
28.	Brunud L, Antunes L, Collinet-Adler S, et al. Acute mesenteric benous thrimbosis: case for nonopereative management. J Vasc surg, 2001, 34(4): 673-679.
29.	Kictchens CS. Evolution of our understanding of the pathophysio logy primary mesenteric venous thrombosis. Am J Surg, 1992, 163 (3): 346-348.

1. 陳建立, 劉彤, 戚峰.腸系膜上靜脈血栓形成的實驗及臨床研究.天津醫科大學, 2012, 81-82.
2. Musemeche CA. A model of instinal ischemia in the neonatal rat utiling superior mesenteric artery occlusion and intraluminal plateletactivating factor. J surg Res, 1995, 58(6): 724-727.
3. Hmoud B, Singal AK, Kamath PS. Mesenteric venous thrombosis. Clin Exp Hepatol, 2014, 4(3): 257-263.
4. 何文梅, 郭虹霞.急性腸系膜靜脈血栓形成8例診治分析.現代診斷與治療, 2014, 25(22): 515-514.
5. 戈小虎, 朱峰.急性腸系膜靜脈血栓形成的診治進展.中國血管外科雜志:電子版, 2015, 7(4): 217-219.
6. Acosta S, Krantz P. Trends in prevalence of fatal surgical diseases at forensic autopsy. ANZ J Surg, 2007, 77(9): 718-721.
7. Condat B, Pessione F, Helene-Denninger M, et al. Recent portal or mesenteric venous thmmbosis:increased recognmon and fhquent recanalization on anticoagulant therapy. Hepatology, 2000, 32(3): 466-470．.
8. 許慶, 鄧淼, 張栩, 等.急性腸系膜靜脈血栓形成的臨床診治策略(附19例報告).臨床和實驗醫學雜志, 2014, 13(13): 1099-1101.
9. 陳軍, 夏培元, 常山, 等.燒傷血清對腸上皮細胞屏障功能損傷的實驗研究.中華創傷雜志, 2002, 18(5):301-304.
10. Krendel MF, Bonder EM. Analysis of actin filament bundle dynamics during contact formation in live epithelial cells. Cell Motil Cytoskeleton, 1999, 43(4): 296-309.
11. 張永珍, 楊立新, 閻西艴, 等.比索洛爾長程口服療法改善冠心病病人的左室舒張功能不全.新藥與臨床, 1997, 16(5): 273-275.
12. 黃震華, 徐濟民.尼卡地平治療心絞痛.新藥與臨床, 1994, 13(3): 169-172.
13. 劉宏鳴, 樊志勝, 曹魯魯.地爾硫靜脈滴注治療冠心病優于丹參注射液.中國新藥與臨床雜志, 1998, 17(6): 355-357.
14. Veitch K, maisin L, Hue L. Trimetazidine ef-fects on the Damage to Mitochondrial Function caused by ischemia and Reperfusion. Am J Cardiol, 1995, 76(6): 25B-30B.
15. Broderick TL, Quinney HA, Barker CC, et al. Beneficial effect of carnitine on mechanical recovery of rat hearts reperfused after a transient period of global ischemia is accompanied by a stimulation of glucose oxidation. Circulation, 1993, 87(3): 972-981.
16. Morin D, Elimadi A, Sapena R, et al. Evidence for the existence of [3H]-trimetazidine binding sites involved in the regulation of the mitochondrial permeability transition pore. Br J Pharmacol, 1998, 123(7): 1385-1394.
17. Tselepis A, Doulias P, htrida E, et al. Tirmetazidine protects lowden sity lipoproteins from oxidation and cultured cells exposed to H₂O₂ from DNA damge. Free Radic Biol Med, 2001, 30(12): 1357-1364.
18. Noble MI, belcheer PR. Drake-Holland AJ. Limitation of infarct size by trimetazidine in the rabbit. Am J Cardiol, 1995, 76(6):41B-44B.
19. Demasion L, Fantini E, Sentex E, et al. Trimetazidine: in vitro influence on heart mitochodrial function. Am J Cardiol, 1995, 96(6): 31B-37B.
20. Croall DE, DeMartino GN. Calcium-activated neutral protease (calpain) system: structure, function, and regulation. Physiol Rev, 1991, 71(3): 813-847.
21. Beynon RJ, Bond JS. Catabolism of intracellular protein: molecular aspects. Am J Physiol, 1986, 251(2 Pt 1): C141-C152.
22. Topham PS, Haydar SA, Kuphal R, et al. Complement-mediated injury reversibly disrupts glomerular epithelial cell actin microfila ments and focaladhesions. Kidney Int, 1999, 55(5): 1763-1775.
23. 顧忠平.曲美他嗪治療缺血性心力衰竭的臨床療效觀察.泰山醫學院學報, 2014, 35(8): 798-799.
24. 楊明.曲美他嗪治療勞累性心絞痛的療效觀察.中西醫結合心血管病雜志, 2015, 3(7): 70-71.
25. 王健, 黃元偉, 魏經漢, 等.曲美他嗪對家兔心肌缺血再灌注損傷的保護作用.浙江大學校報, 2003, 32(3): 219-222.
26. 李選, 歐陽強, 蕭湘生.腸系膜靜脈血栓的介入治療.介入放射學雜志, 2006, 15(4): 202-205.
27. Rhee Ry, Gloviczki P, Mesenteric venous thrombosis. Surg Clin North am, 1997, 77(2): 327-338.
28. Brunud L, Antunes L, Collinet-Adler S, et al. Acute mesenteric benous thrimbosis: case for nonopereative management. J Vasc surg, 2001, 34(4): 673-679.
29. Kictchens CS. Evolution of our understanding of the pathophysio logy primary mesenteric venous thrombosis. Am J Surg, 1992, 163 (3): 346-348.

Previous Article
法國普胸外科訪學見聞
Next Article
大劑量放化療后局部進展挽救性肺切除術五例

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Protective Effect of Low-Molecular-Weight Heparin Calcium Combined with Trimetazi-dine on Intestinal Smooth Muscle of Intestinal Acute Mesangial Vein Thrombosis in Rats

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content