Significance of Glucocorticoid for Rat Liver Transplantation Model_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 LIUBo-qiang ,  RANJiang-hua , LIWen-kai , ZHENGKe-pu

Department of Hepatobiliary Surgery, Affiliated Ganmei Hospital of Kunming Medical University(The First People's Hospital of Kunming), Kunming 650011, Yunnan Province, China;

Corresponding?author：

RANJiang-hua, Email: rjh2u@163.com

Keywords：

Rat; Liver transplantation; Glucocorticoid

DOI：

10.7507/1007-9424.20160148

Video：

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Objective To explore significance of glucocorticoid for rat liver transplantation model. Methods Two hundred rats were randomly divided into experimental group and control group. The experimental group rats were injected with sulfate atropine resulotion 0.1 mg/kg, cephazolin-Na 0.3 g/kg and hydrocortisone 5 mg/kg while the control group rats were injected with sulfate atropine resulotion 0.1 mg/kg, cephazolin-Na 0.3 g/kg and equal volume of normal saline with glucocorticoid at 30 min before operation. The donor surgery time, repairing liver time, recipient surgery time, anhepatic phase, and 1-day, 3-day and 7-day survival rates were compared between these two groups. Results The donor surgery time, repairing liver time, recipient surgery time, and anhepatic phase had no significant differences between the experimental group and control group (P>0.05), while the 1-day, 3-day and 7-day survival rates of the experimental group were significantly higher than those of the control group [96% (48/50) versus 80% (40/50), P<0.05; 92% (46/50) versus 72% (36/50), P<0.05; 90% (45/50) versus 54% (27/50), P<0.05]. Conclusion Usage of glucocorticoid might contribute to improve survival rate of rat liver transplantation model.

Citation： LIUBo-qiang, RANJiang-hua, LIWen-kai, ZHENGKe-pu. Significance of Glucocorticoid for Rat Liver Transplantation Model. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(5): 552-555. doi: 10.7507/1007-9424.20160148 Copy

1.	?Kamada N, Calne RY. A surgical experience with five hundred thirty liver transplants in the rat. Surgery, 1983, 93(1 Pt 1):64-69.
2.	李立. 大鼠肝移植圖譜. 北京:人民衛生出版社, 2012:1-46.
3.	劉劍, 李立, 張升寧, 等. 大鼠原位肝移植術后早期死亡的原因分析及處理策略. 中國普外基礎與臨床雜志, 2014, 21(12):1524-1530.
4.	姚坤厚, 高毅, 潘明新, 等. 大鼠原位肝移植急性排斥模型的建立. 實用醫學雜志, 2008, 24(8):1304-1306.
5.	Tan F, Chen Z, Zhao Y, et al. Novel technique for suprahepatic vena cava reconstruction in rat orthotopic liver transplantation. Microsurgery, 2005, 25(7):556-560.
6.	Matevossian E, Doll D, Hüser N, et al. Liver transplantation in the rat:single-center experience with technique, long-term survival, and functional and histologic findings.Transplant Proc, 2009, 41(6):2631-2636.
7.	Kamada N, Calne RY. Orthotopic liver transplantation in the rat:Technique using cuff for portal vein anastomosis and biliary drainage. Transplantation, 1979, 28(1):47-50.
8.	Miyata M, Fischer H, Fuhs M, et al. A simple method for orthotopic liver transplantation in the rat:Cuff technique for three vascular anastomoses. Transplantation, 1980, 30(5):335-338.
9.	Inoue S, Tahara K, Shimizu H, et al. Rat liver transplantation for total vascular reconstruction, using a suture method. Microsurgery, 2003, 23(5):470-475.
10.	李鏡鋒, 丁煥新, 白建華, 等. 無心跳供肝大鼠肝移植模型的手術操作技巧探討. 中國普外基礎與臨床雜志, 2010, 17(8):785-788.
11.	劉靜, 李江, 張升寧, 等. 改良法構建大鼠減體積肝移植模型. 中國組織工程研究與臨床康復, 2010, 14(18):3252-3257.
12.	李軍, 竇科峰, 張洪濤, 等. 供肝快速切取法大鼠原位肝移植模型的建立. 中國普通外科雜志, 2006, 15(7):500-503.
13.	史源, 蔣安, 張勇, 等. 一種犬肝移植大血管快速重建的新方法. 中國修復重建外科雜志, 2007, 21(4):420-423.
14.	李萌, 潘崎, 程穎, 等. 褪黑素對大鼠移植肝組織GRP-78及CHOP表達的影響. 器官移植, 2015, 6(4):262-267.
15.	任秋平, 羅艷麗. 肝移植受者延續性護理需求及相關因素分析. 華西醫學, 2015, 30(9):1720-1723.
16.	Grogan TA. Liver transplantation:issues and nursing care requirements. Crit Care Nurs Clin North, 2011, 23(3):443-456.
17.	Bulatao IG, Heckman MG, Rawal B, et al. Avoiding stay in the intensive care unit after liver transplantation:a score to assign location of care. Am J Transplant, 2014, 14(9):2088-2096.
18.	俞棟棟, 周琳, 鄭樹森. 肝移植臨床可操作性耐受的研究現狀及展望. 中華移植雜志:電子版, 2013, 7(2):103-107.
19.	夏瑾, 夏洋, 龔建平. 肝移植免疫抑制治療臨床應用進展. 中華移植雜志:電子版, 2013, 7(3):162-167.
20.	Zitta S, Schaffellner S, Gutschi J, et al. The effect of mammalian target of rapamycin versus calcineurin inhibitor-based immunosuppression on measured versus estimated glomerular filtration rate after orthotopic liver transplantation. Transplantation, 2015, 99(6):1250-1256.
21.	Charlton MR. Improving long-term outcomes after liver transplantation. Clin Liver Dis, 2014, 18(3):717-730.
22.	潘穎, 楊劼, 劉國強, 等. 肝移植患者用藥分析. 河北醫藥, 2015, 37(8):1244-1246.
23.	Mouloudi E, Massa E, Papadopoulos S, et al. Bloodstream infections caused by carbapenemase-producing Klebsiella pneumoniae among intensive care unit patients after orthotopic liver transplantation:risk factors for infection and impact of resistance on outcomes. Transplant Proc, 2014, 46(9):3216-3218.
24.	Kim SI. Bacterial infection after liver transplantation. World J Gastroenterol, 2014, 20(20):6211-6220.
25.	Largiadèr F. Immunosuppression during clinical and experimental organ transplantation. Naturwissenschaften, 1968, 55(9):429-434.
26.	?berg F. Role of budesonide in liver transplantation. Transpl Immunol, 2014, 30(4):178-179.
27.	石炳毅,朱志軍,朱曉峰. 他克莫司在臨床肝移植中的應用指南. 實用器官移植電子雜志, 2015, 31(3):696-698.
28.	魏曉晨, 朱立勤, 王春革. 激素對肝移植患者術后感染影響的Meta分析. 中國醫院藥學雜志, 2014, 34(9):735-742.
29.	李銳鋒. 糖皮質激素在阻止急性肝衰竭發生中的作用與機制研究. 第三軍醫大學, 2014:14-19.
30.	徐建國, 江偉. 腎上腺糖皮質激素在圍術期應用的專家共識. 臨床麻醉學雜志, 2013, 29(2):200-204.

1. ?Kamada N, Calne RY. A surgical experience with five hundred thirty liver transplants in the rat. Surgery, 1983, 93(1 Pt 1):64-69.
2. 李立. 大鼠肝移植圖譜. 北京:人民衛生出版社, 2012:1-46.
3. 劉劍, 李立, 張升寧, 等. 大鼠原位肝移植術后早期死亡的原因分析及處理策略. 中國普外基礎與臨床雜志, 2014, 21(12):1524-1530.
4. 姚坤厚, 高毅, 潘明新, 等. 大鼠原位肝移植急性排斥模型的建立. 實用醫學雜志, 2008, 24(8):1304-1306.
5. Tan F, Chen Z, Zhao Y, et al. Novel technique for suprahepatic vena cava reconstruction in rat orthotopic liver transplantation. Microsurgery, 2005, 25(7):556-560.
6. Matevossian E, Doll D, Hüser N, et al. Liver transplantation in the rat:single-center experience with technique, long-term survival, and functional and histologic findings.Transplant Proc, 2009, 41(6):2631-2636.
7. Kamada N, Calne RY. Orthotopic liver transplantation in the rat:Technique using cuff for portal vein anastomosis and biliary drainage. Transplantation, 1979, 28(1):47-50.
8. Miyata M, Fischer H, Fuhs M, et al. A simple method for orthotopic liver transplantation in the rat:Cuff technique for three vascular anastomoses. Transplantation, 1980, 30(5):335-338.
9. Inoue S, Tahara K, Shimizu H, et al. Rat liver transplantation for total vascular reconstruction, using a suture method. Microsurgery, 2003, 23(5):470-475.
10. 李鏡鋒, 丁煥新, 白建華, 等. 無心跳供肝大鼠肝移植模型的手術操作技巧探討. 中國普外基礎與臨床雜志, 2010, 17(8):785-788.
11. 劉靜, 李江, 張升寧, 等. 改良法構建大鼠減體積肝移植模型. 中國組織工程研究與臨床康復, 2010, 14(18):3252-3257.
12. 李軍, 竇科峰, 張洪濤, 等. 供肝快速切取法大鼠原位肝移植模型的建立. 中國普通外科雜志, 2006, 15(7):500-503.
13. 史源, 蔣安, 張勇, 等. 一種犬肝移植大血管快速重建的新方法. 中國修復重建外科雜志, 2007, 21(4):420-423.
14. 李萌, 潘崎, 程穎, 等. 褪黑素對大鼠移植肝組織GRP-78及CHOP表達的影響. 器官移植, 2015, 6(4):262-267.
15. 任秋平, 羅艷麗. 肝移植受者延續性護理需求及相關因素分析. 華西醫學, 2015, 30(9):1720-1723.
16. Grogan TA. Liver transplantation:issues and nursing care requirements. Crit Care Nurs Clin North, 2011, 23(3):443-456.
17. Bulatao IG, Heckman MG, Rawal B, et al. Avoiding stay in the intensive care unit after liver transplantation:a score to assign location of care. Am J Transplant, 2014, 14(9):2088-2096.
18. 俞棟棟, 周琳, 鄭樹森. 肝移植臨床可操作性耐受的研究現狀及展望. 中華移植雜志:電子版, 2013, 7(2):103-107.
19. 夏瑾, 夏洋, 龔建平. 肝移植免疫抑制治療臨床應用進展. 中華移植雜志:電子版, 2013, 7(3):162-167.
20. Zitta S, Schaffellner S, Gutschi J, et al. The effect of mammalian target of rapamycin versus calcineurin inhibitor-based immunosuppression on measured versus estimated glomerular filtration rate after orthotopic liver transplantation. Transplantation, 2015, 99(6):1250-1256.
21. Charlton MR. Improving long-term outcomes after liver transplantation. Clin Liver Dis, 2014, 18(3):717-730.
22. 潘穎, 楊劼, 劉國強, 等. 肝移植患者用藥分析. 河北醫藥, 2015, 37(8):1244-1246.
23. Mouloudi E, Massa E, Papadopoulos S, et al. Bloodstream infections caused by carbapenemase-producing Klebsiella pneumoniae among intensive care unit patients after orthotopic liver transplantation:risk factors for infection and impact of resistance on outcomes. Transplant Proc, 2014, 46(9):3216-3218.
24. Kim SI. Bacterial infection after liver transplantation. World J Gastroenterol, 2014, 20(20):6211-6220.
25. Largiadèr F. Immunosuppression during clinical and experimental organ transplantation. Naturwissenschaften, 1968, 55(9):429-434.
26. ?berg F. Role of budesonide in liver transplantation. Transpl Immunol, 2014, 30(4):178-179.
27. 石炳毅,朱志軍,朱曉峰. 他克莫司在臨床肝移植中的應用指南. 實用器官移植電子雜志, 2015, 31(3):696-698.
28. 魏曉晨, 朱立勤, 王春革. 激素對肝移植患者術后感染影響的Meta分析. 中國醫院藥學雜志, 2014, 34(9):735-742.
29. 李銳鋒. 糖皮質激素在阻止急性肝衰竭發生中的作用與機制研究. 第三軍醫大學, 2014:14-19.
30. 徐建國, 江偉. 腎上腺糖皮質激素在圍術期應用的專家共識. 臨床麻醉學雜志, 2013, 29(2):200-204.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Significance of Glucocorticoid for Rat Liver Transplantation Model

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