Experimental Research of Laryngeal Muscle and Pathophysiology of Recurrent Laryngeal Nerve after Unilateral Recurrent Laryngeal Nerve Transection for Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 QUJun , SHENYe , WANGWen-jie , HUANGZhong-ming , WANGShi-guang ,  WUTao

Department of General Surgery, Shanghai Fengxian District Central Hospital, Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus, Shanghai 201499, China;

Corresponding?author：

WUTao, Email: hijump46@sohu.com

Keywords：

Recurrent laryngeal nerve; Transection; Brain-derived neurotrophic factor; Vocal cord palsy; Nerve regeneration; Rat

DOI：

10.7507/1007-9424.20160366

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective The aim of this study was to evaluate the repair effect of spontaneous reinnervation in rats underwent recurrent laryngeal nerve (RLN) transection. Methods Thirty male Wistar rats (340-360 g) were divided into experiment group (n=15) and blank control group (n=15), and then 15 rats of these 2 groups were divided into 3 time point groups equally:4 weeks group, 8 weeks group, and 12 weeks group. Fifteen rats of experiment group underwent right RLN transection with excision of a 5 mm segment, and other 15 rats of blank control group exposed RLN only, without transection. Grade of vocalization, maximum angle of arytenoid cartilage, axon number of distal part of RLN, and expression of the brain-derived neurotrophic factor (BDNF) in right thyroarytenoid muscle were evaluated at different time points, including 4, 8, and 12 weeks after operation. Results Grade of vocalization, maximum angle of arytenoid cartilage, axon numbers of distal part of RLN, and the expression of BDNF in the right thyroarytenoid muscle of experiment group were all lower than those corresponding index of blank control group (P < 0.05), and these indexes of experiment group were restored gradually with time, but failed to reach normal level during the observed time. Conclusions Even though spontaneous reinnervation is presented after RLN injury, but the effect is unsatisfactory.

Citation： QUJun, SHENYe, WANGWen-jie, HUANGZhong-ming, WANGShi-guang, WUTao. Experimental Research of Laryngeal Muscle and Pathophysiology of Recurrent Laryngeal Nerve after Unilateral Recurrent Laryngeal Nerve Transection for Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2016, 23(12): 1437-1442. doi: 10.7507/1007-9424.20160366 Copy

1.	Brown TJ, Pittman AL, Monaco GN, et al. Androgen treatment and recovery of function following recurrent laryngeal nerve injury in the rat. Restor Neurol Neurosci, 2013, 31(2):169-176.
2.	Moro K, Shiotani A, Watabe K, et al. Adenoviral gene transfer of BDNF and GDNF synergistically prevent motoneuron loss in the nucleus ambiguus. Brain Res, 2006, 1076(1):1-8.
3.	Miyamaru S, Kumai Y, Ito T, et al. Effects of long-term denervation on the rat thyroarytenoid muscle. Laryngoscope, 2008, 118(7):1318-1323.
4.	Fang TJ, Li HY, Gliklich RE, et al. Quality of life measures and predictors for adults with unilateral vocal cord paralysis. Laryngoscope, 2008, 118(10):1837-1841.
5.	馮云, 楊大章, 呂秋萍, 等.喉返神經修復術及非喉返神經修復術治療聲帶麻痹.山東大學耳鼻喉眼學報, 2015, 29(3):54-58.
6.	Araki K, Shiotani A, Watabe K, et al. Adenoviral GDNF gene transfer enhances neurofunctional recovery after recurrent laryngeal nerve injury. Gene Ther, 2006, 13(4):296-303.
7.	王保鑫, 董頻.神經營養因子對喉返神經損傷再生的研究進展.中國眼耳鼻喉科雜志, 2014, 14(1):52-55.
8.	李孟, 陳世彩, 陳東輝, 等.肝細胞生長因子對喉返神經損傷后再生的作用.聽力學及言語疾病雜志, 2012, 20(5):467-471.
9.	Levi-Montalcini R. The nerve growth factor 35 years later. Science, 1987, 237(4819):1154-1162.
10.	曾維軍, 周文勝, 王佳.神經營養因子和生長因子在神經干細胞中的研究進展.臨床與病理雜志, 2015, 35(3):484-489.
11.	Han Q, Jin W, Xiao Z, et al. The promotion of neural regeneration in an extreme rat spinal cord injury model using a collagen scaffold containing a collagen binding neuroprotective protein and an EGFR neutralizing antibody. Biomaterials, 2010, 31(35):9212-9220.
12.	何雙八, 孫敬武, 孫東東.單側喉返神經切斷后甲杓肌結構與酶學變化的實驗性研究.安徽醫科大學學報, 2005, 40(3):231-233.
13.	徐文, 趙功偉, 胡慧英, 等.喉返神經損傷后喉肌病理生理研究.臨床耳鼻咽喉頭頸外科雜志, 2009, 23(9):403-406.
14.	Vega-Cordova X, Cosenza NM, Helfert RH, et al. Neurotrophin expression of laryngeal muscles in response to recurrent laryngeal nerve transection. Laryngoscope, 2010, 120(8):1591-1596.
15.	施劍斌, 鄭宏良, 陳世彩, 等.改良杓狀軟骨內收術治療單側聲帶麻痹.聽力學及言語疾病雜志, 2010, 18(1):29-32.
16.	張品一, 張濱, 劉垚, 等.甲狀腺手術中喉返神經變異的臨床研究.中華普通外科雜志, 2013, 28(6):431-435.
17.	申曦, 李小毅, 劉躍武, 等.非返性喉返神經的解剖特點及術中損傷的預防.中國普外基礎與臨床雜志, 2013, 20(1):71-76.
18.	Zábrodsky M, Bou?ek J, Kastner J, et al. Immediate revision in patients with bilateral recurrent laryngeal nerve palsy after thyroid and parathyroid surgery. How worthy is it? Acta Otorhinolaryngol Ital, 2012, 32(4):222-228.
19.	Sunderland S. A classification of peripheral nerve injuries producing loss of function. Brain, 1951, 74(4):491-516.
20.	Woodson GE, Hughes LF, Helfert R. Quantitative assessment of laryngeal muscle morphology after recurrent laryngeal nerve injury:right vs. left differences. Laryngoscope, 2008, 118(10):1768-1770.
21.	侯麗珍, 韓德民, 徐文, 等.喉返神經麻痹的肌電特點及相關研究.臨床耳鼻咽喉頭頸外科雜志, 2007, 21(15):673-678.
22.	馬情情, 孫麗, 鄭宏良, 等.特發性聲帶麻痹患者環杓后肌肌纖維形態觀察.聽力學及言語疾病雜志, 2015, 23(3):256-260.
23.	Aoyama T, Kumai Y, Yumoto E, et al. Effects of nerve-muscle pedicle on immobile rat vocal folds in the presence of partial innervation. Ann Otol Rhinol Laryngol, 2010, 119(12):823-829.
24.	Pitman MJ, Weissbrod P, Roark R, et al. Electromyographic and histologic evolution of the recurrent laryngeal nerve from transection and anastomosis to mature reinnervation. Laryngoscope, 2011, 121(2):325-331.
25.	Toya Y, Kumai Y, Minoda R, et al. Modulation of nerve fibers in the rat thyroarytenoid muscle following recurrent laryngeal nerve injury. Acta Otolaryngol, 2012, 132(3):305-313.
26.	Sharma N, Marzo SJ, Jones KJ, et al. Electrical stimulation and testosterone differentially enhance expression of regeneration-associated genes. Exp Neurol, 2010, 223(1):183-191.

1. Brown TJ, Pittman AL, Monaco GN, et al. Androgen treatment and recovery of function following recurrent laryngeal nerve injury in the rat. Restor Neurol Neurosci, 2013, 31(2):169-176.
2. Moro K, Shiotani A, Watabe K, et al. Adenoviral gene transfer of BDNF and GDNF synergistically prevent motoneuron loss in the nucleus ambiguus. Brain Res, 2006, 1076(1):1-8.
3. Miyamaru S, Kumai Y, Ito T, et al. Effects of long-term denervation on the rat thyroarytenoid muscle. Laryngoscope, 2008, 118(7):1318-1323.
4. Fang TJ, Li HY, Gliklich RE, et al. Quality of life measures and predictors for adults with unilateral vocal cord paralysis. Laryngoscope, 2008, 118(10):1837-1841.
5. 馮云, 楊大章, 呂秋萍, 等.喉返神經修復術及非喉返神經修復術治療聲帶麻痹.山東大學耳鼻喉眼學報, 2015, 29(3):54-58.
6. Araki K, Shiotani A, Watabe K, et al. Adenoviral GDNF gene transfer enhances neurofunctional recovery after recurrent laryngeal nerve injury. Gene Ther, 2006, 13(4):296-303.
7. 王保鑫, 董頻.神經營養因子對喉返神經損傷再生的研究進展.中國眼耳鼻喉科雜志, 2014, 14(1):52-55.
8. 李孟, 陳世彩, 陳東輝, 等.肝細胞生長因子對喉返神經損傷后再生的作用.聽力學及言語疾病雜志, 2012, 20(5):467-471.
9. Levi-Montalcini R. The nerve growth factor 35 years later. Science, 1987, 237(4819):1154-1162.
10. 曾維軍, 周文勝, 王佳.神經營養因子和生長因子在神經干細胞中的研究進展.臨床與病理雜志, 2015, 35(3):484-489.
11. Han Q, Jin W, Xiao Z, et al. The promotion of neural regeneration in an extreme rat spinal cord injury model using a collagen scaffold containing a collagen binding neuroprotective protein and an EGFR neutralizing antibody. Biomaterials, 2010, 31(35):9212-9220.
12. 何雙八, 孫敬武, 孫東東.單側喉返神經切斷后甲杓肌結構與酶學變化的實驗性研究.安徽醫科大學學報, 2005, 40(3):231-233.
13. 徐文, 趙功偉, 胡慧英, 等.喉返神經損傷后喉肌病理生理研究.臨床耳鼻咽喉頭頸外科雜志, 2009, 23(9):403-406.
14. Vega-Cordova X, Cosenza NM, Helfert RH, et al. Neurotrophin expression of laryngeal muscles in response to recurrent laryngeal nerve transection. Laryngoscope, 2010, 120(8):1591-1596.
15. 施劍斌, 鄭宏良, 陳世彩, 等.改良杓狀軟骨內收術治療單側聲帶麻痹.聽力學及言語疾病雜志, 2010, 18(1):29-32.
16. 張品一, 張濱, 劉垚, 等.甲狀腺手術中喉返神經變異的臨床研究.中華普通外科雜志, 2013, 28(6):431-435.
17. 申曦, 李小毅, 劉躍武, 等.非返性喉返神經的解剖特點及術中損傷的預防.中國普外基礎與臨床雜志, 2013, 20(1):71-76.
18. Zábrodsky M, Bou?ek J, Kastner J, et al. Immediate revision in patients with bilateral recurrent laryngeal nerve palsy after thyroid and parathyroid surgery. How worthy is it? Acta Otorhinolaryngol Ital, 2012, 32(4):222-228.
19. Sunderland S. A classification of peripheral nerve injuries producing loss of function. Brain, 1951, 74(4):491-516.
20. Woodson GE, Hughes LF, Helfert R. Quantitative assessment of laryngeal muscle morphology after recurrent laryngeal nerve injury:right vs. left differences. Laryngoscope, 2008, 118(10):1768-1770.
21. 侯麗珍, 韓德民, 徐文, 等.喉返神經麻痹的肌電特點及相關研究.臨床耳鼻咽喉頭頸外科雜志, 2007, 21(15):673-678.
22. 馬情情, 孫麗, 鄭宏良, 等.特發性聲帶麻痹患者環杓后肌肌纖維形態觀察.聽力學及言語疾病雜志, 2015, 23(3):256-260.
23. Aoyama T, Kumai Y, Yumoto E, et al. Effects of nerve-muscle pedicle on immobile rat vocal folds in the presence of partial innervation. Ann Otol Rhinol Laryngol, 2010, 119(12):823-829.
24. Pitman MJ, Weissbrod P, Roark R, et al. Electromyographic and histologic evolution of the recurrent laryngeal nerve from transection and anastomosis to mature reinnervation. Laryngoscope, 2011, 121(2):325-331.
25. Toya Y, Kumai Y, Minoda R, et al. Modulation of nerve fibers in the rat thyroarytenoid muscle following recurrent laryngeal nerve injury. Acta Otolaryngol, 2012, 132(3):305-313.
26. Sharma N, Marzo SJ, Jones KJ, et al. Electrical stimulation and testosterone differentially enhance expression of regeneration-associated genes. Exp Neurol, 2010, 223(1):183-191.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Experimental Research of Laryngeal Muscle and Pathophysiology of Recurrent Laryngeal Nerve after Unilateral Recurrent Laryngeal Nerve Transection for Rats

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content