Effect of simulated microgravity mean on morphology and secretory function in cultured parathyroid cells of rat_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHAO Dajiang , LI Tongchang , WANG Biao ,  DUAN Xiuqing

Department of Breast Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, P.R.China;

Corresponding?author：

DUAN Xiuqing, Email: dc_95@aliyun.com

Keywords：

parathyroid cells; microgravity; cell culture; polyglycolic acid

DOI：

10.7507/1007-9424.201606022

Video：

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Objective To culture primary parathyroid cells by mean of simulated microgravity, observe their basic morphological characteristics, study survival rate and secretory function of parathyroid cells, and explore more excellent culture mean of parathyroid cells. Methods There were 37 male Wistar rats, the body weight was 150–200 g. The rat was intraperitoneally injected with 1% pentobarbital sodium (50 mg/kg). The parathyroid glands were surgically excised and identified pathologically. The parathyroid gland cells were got and digested them with collagenase Ⅱ, which were divided into three groups: conventional culture group (simple parathyroid cells were cultured), polyglycolic acid (PGA) scaffold culture group (the parathyroid cells were cultured on the PGA scaffold), and simulated microgravity culture group (the parathyroid cells and PGA scaffolds were cultured in simulated microgravity environment). The parathyroid cells were cultured for 1, 3, 5 or 7 days in different culture conditions, then the parathyroid hormone (PTH) was measured, morphological characteristics of the parathyroid cell was observed under phase contrast microscope, survival rate of the parathyroid cells was calculated by acridine orange/propidium iodide staining. Results The parathyroid cell morphologies of most cells were well and center of part of cell mass was necrosis on day 7 in the conventional culture group. The most parathyroid cells were spreading toward the poles along the PGA cell scaffold in the longitudinal direction and the adjacent stents were connected by extracellular matrix on day 7 in the PGA scaffold culture group. The parathyroid cells cultured under the simulated microgravity were got round and formed clusters on day 7 in the simulated microgravity culture group. Compared with the other two groups on day 7, the PTH and the survival rate of the parathyroid cells were significantly higher in the simulated microgravity culture group (P<0.05). Conclusions Parathyroid cells cultured in simulated microgravity environment could maintain better morphology, survival rate is higher, and secretory function is better. Therefore, parathyroid cells cultured in simulated microgravity could be used as good donor cell for treatment of hypoparathyroidism. PGA scaffold could be used as a good carrier for culture of parathyroid cell.

Citation： ZHAO Dajiang, LI Tongchang, WANG Biao, DUAN Xiuqing. Effect of simulated microgravity mean on morphology and secretory function in cultured parathyroid cells of rat. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2017, 24(2): 179-184. doi: 10.7507/1007-9424.201606022 Copy

1.	?段秀慶, 宋純, 許評, 等. 去甲狀旁腺模型鼠的制備. 中國普外基礎與臨床雜志, 2005, 12(4): 393-394.
2.	高慶軍, 趙代偉, 王南鵬, 等. 同種異體甲狀旁腺組織移植治療甲狀旁腺功能低下的實驗研究. 中國普外基礎與臨床雜志, 2012 , 19(10): 1079-1084.
3.	王志宏, 張浩. 異體甲狀旁腺移植現狀與展望. 中國實用外科雜志, 2012, 32(5): 412-414.
4.	王存濤, 郭衛東, 鄒浩, 等. 甲狀旁腺移植臨床及實驗研究新進展. 中華普外科手術學雜志(電子版), 2014, 8(1): 87-89.
5.	孫海晨, 劉爽, 崔葉青, 等. 成人甲狀旁腺細胞的培養方法及其分泌功能研究. 中國普外基礎與臨床雜志, 2014, 21(9): 1120-1123.
6.	Freed LE, Langer R, Martin I,et al. Tissue engineering of cartilage in space. Proc Nati Acad Sci, 1997, 94(25): 13885-13890.
7.	Unsworth BR, Lelkes PI. Growing tissues in microgravity. Nat Med, 1998, 4(8): 901-907.
8.	Hammond TG, Hammond JM. Optimized suspension culture: the rotating-wall vessel. Am J Physiol Renal Physiol, 2001, 281(1): F12-F25.
9.	Mitteregger R, Vogt G, Rossmanith E,et al. Rotary cell culture system (RCCS): a new method for cultivating hepatocytes on microcarriers. Int J Artif organs, 1999, 22(12): 816-822.
10.	Khaoustov VI, Darlington GJ, Soriano HE,et al. Induction of three-dimensional assembly of human liver cells by simulated microgravity. In Vitro Cell Dev Biol Anim, 1999, 35(9): 501-509.
11.	Khaoustov VI, Risin D, Pellis NR,et al. Microarray analysis of genes differentially expressed in HepG2 cells cultured in simulated microgravity: preliminary report. In Vitro Cell Dev Biol Anim, 2001, 37(2): 84-88.
12.	Margolis L, Hatfill S, Chuaqui R. Long term organ culture of human prostate tisse in NASA-designed rotating wall bioreactor. J Urol, 1999, 161(1): 290-297.
13.	陳輝, 李斌, 王健, 等. 膠原支架復合兔骨髓間充質干細胞體外模擬的微重力培養. 中國組織工程研究與臨床康復, 2010, 14(34): 6308-6312.
14.	Ben-Ze’ev A, Robinson GS, Bucher NL,et al. Cell-cell and cell-matrix interactions differentially regulate the expression of hepatic and cytoskeletal genes in primary cultures of rat hepatocytes. Proc Nati Acad Sci, 1988, 85(7): 2161-2165.
15.	段秀慶, 李永寧, 宋純. 大鼠甲狀旁腺細胞體外培養及功能的實驗研究. 中國現代普通外科進展, 2008, 11(5): 389-391.

1. ?段秀慶, 宋純, 許評, 等. 去甲狀旁腺模型鼠的制備. 中國普外基礎與臨床雜志, 2005, 12(4): 393-394.
2. 高慶軍, 趙代偉, 王南鵬, 等. 同種異體甲狀旁腺組織移植治療甲狀旁腺功能低下的實驗研究. 中國普外基礎與臨床雜志, 2012 , 19(10): 1079-1084.
3. 王志宏, 張浩. 異體甲狀旁腺移植現狀與展望. 中國實用外科雜志, 2012, 32(5): 412-414.
4. 王存濤, 郭衛東, 鄒浩, 等. 甲狀旁腺移植臨床及實驗研究新進展. 中華普外科手術學雜志(電子版), 2014, 8(1): 87-89.
5. 孫海晨, 劉爽, 崔葉青, 等. 成人甲狀旁腺細胞的培養方法及其分泌功能研究. 中國普外基礎與臨床雜志, 2014, 21(9): 1120-1123.
6. Freed LE, Langer R, Martin I,et al. Tissue engineering of cartilage in space. Proc Nati Acad Sci, 1997, 94(25): 13885-13890.
7. Unsworth BR, Lelkes PI. Growing tissues in microgravity. Nat Med, 1998, 4(8): 901-907.
8. Hammond TG, Hammond JM. Optimized suspension culture: the rotating-wall vessel. Am J Physiol Renal Physiol, 2001, 281(1): F12-F25.
9. Mitteregger R, Vogt G, Rossmanith E,et al. Rotary cell culture system (RCCS): a new method for cultivating hepatocytes on microcarriers. Int J Artif organs, 1999, 22(12): 816-822.
10. Khaoustov VI, Darlington GJ, Soriano HE,et al. Induction of three-dimensional assembly of human liver cells by simulated microgravity. In Vitro Cell Dev Biol Anim, 1999, 35(9): 501-509.
11. Khaoustov VI, Risin D, Pellis NR,et al. Microarray analysis of genes differentially expressed in HepG2 cells cultured in simulated microgravity: preliminary report. In Vitro Cell Dev Biol Anim, 2001, 37(2): 84-88.
12. Margolis L, Hatfill S, Chuaqui R. Long term organ culture of human prostate tisse in NASA-designed rotating wall bioreactor. J Urol, 1999, 161(1): 290-297.
13. 陳輝, 李斌, 王健, 等. 膠原支架復合兔骨髓間充質干細胞體外模擬的微重力培養. 中國組織工程研究與臨床康復, 2010, 14(34): 6308-6312.
14. Ben-Ze’ev A, Robinson GS, Bucher NL,et al. Cell-cell and cell-matrix interactions differentially regulate the expression of hepatic and cytoskeletal genes in primary cultures of rat hepatocytes. Proc Nati Acad Sci, 1988, 85(7): 2161-2165.
15. 段秀慶, 李永寧, 宋純. 大鼠甲狀旁腺細胞體外培養及功能的實驗研究. 中國現代普通外科進展, 2008, 11(5): 389-391.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of simulated microgravity mean on morphology and secretory function in cultured parathyroid cells of rat

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