Hypoxia condition can enhance proliferation, adhesion, migration, and viability abilities of bone morrow-derived endothelial progenitor cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

SONG Lipo ¹ , ZHANG Jian ¹ ,  GU Yongquan ¹ , CHEN Bing ² , GUO Lianrui ¹ , WANG Chunmei ¹ , WU Yingfeng ¹ , LUO Tao ¹ , CUI Shijun ¹ , WANG Zhonggao ¹

1. Department of Vascular Surgery, Xuanwu Hospital, Institute of Vascular Surgery, Capital Medical University, Beijing 100053, P. R. China;
2. Department of Vascular Surgery, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, P. R. China;

Corresponding?author：

GU Yongquan, Email: gu-yq@263.net

Keywords：

neovacularization; hypoxia; bone morrow-derived stem cell; endothelial progenitor cell; critical limb ischemia

DOI：

10.7507/1007-9424.201710088

Video：

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Objective To evaluate effect of hypoxia condition (1% or 5% oxygen concentration) on proliferation, adhesion, migration, or viability ability of bone morrow-derived endothelial progenitor cells (EPCs). Methods The bone marrow mononuclear cells of SD rat were acquired with density gradient centrifugation method. They were cultured, induced, and differentiated to the EPCs. Then they were cultured respectively in three different oxygen concentrations (1%, 5%, or 21%). On the 3rd day and the 7th day, the effects of the different oxygen concentrations (1%, 5%, or 21%) on the EPCs’ neovascularization characteristics (including proliferation, adhesion, migration, and viability abilities) were evaluated. Results Whether cultured for the 3rd day or 7th day, the proliferation, adhesion, migration, and viability abilities of the cultured cells in the 1% and 5% oxygen concentrations were significantly better than those of the cultured cells in the 21% oxygen concentration (all P<0.05). Except for the proliferation ability of the cultured cells in the 5% oxygen concentration was significantly better than that of the cultured cells in the 1% oxygen concentration (P<0.05) on the 3rd day, and the adhesion ability on the 3rd day and the proliferation ability on the 7th day had no significantly differences, the other abilities (adhesion, migration, and viability abilities) of the cultured cells in the 1% oxygen concentration were significantly better than those of the cultured cells in the 5% oxygen concentration (allP<0.05). Conclusion Different oxygen concentration has an effect on proliferation, adhesion, migration, or viability ability of bone morrow-derived EPCs, appropriate hypoxia condition (1% or 5% oxygen concentration ) can enhance these abilities.

Citation： SONG Lipo, ZHANG Jian, GU Yongquan, CHEN Bing, GUO Lianrui, WANG Chunmei, WU Yingfeng, LUO Tao, CUI Shijun, WANG Zhonggao. Hypoxia condition can enhance proliferation, adhesion, migration, and viability abilities of bone morrow-derived endothelial progenitor cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2018, 25(5): 528-533. doi: 10.7507/1007-9424.201710088 Copy

1.	Asahara T, Murohara T, Sullivan A, et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science, 1997, 275(5302): 964-967.
2.	白潔, 孟軍, 蔡澤民, 等. 高脂蛋白(a)的冠心病患者內皮祖細胞功能受損. 中國動脈硬化雜志, 2015, 23(4): 384-388.
3.	Yu CG, Zhang N, Yuan SS, et al. Endothelial progenitor cells in diabetic microvascular complications: friends or foes. Stem Cells Int, 2016, 2016:1803989. doi: 10.1155/2016/1803989. Epub 2016 May 29.
4.	Kang H, Ma X, Liu J, et al. High glucose-induced endothelial progenitor cell dysfunction. Diab Vasc Dis Res, 2017, 14(5): 381-394.
5.	Li TB, Zhang YZ, Liu WQ, et al. Correlation between NADPH oxidase-mediated oxidative stress and dysfunction of endothelial progenitor cell in hyperlipidemic patients. Korean J Intern Med, 2017, doi: 10.3904/kjim.2016.140.[Epub ahead of print].
6.	King TF, McDermott JH. Endothelial progenitor cells and cardiovascular disease. J Stem Cells, 2014, 9(2): 93-106.
7.	徐竹, 諸葛啟釧, 黃李潔. 干細胞3D支架的研究進展. 中國生物工程雜志, 2017, 37(9): 112-117.
8.	Mohyeldin A, Garzón-Muvdi T, Qui?ones-Hinojosa A. Oxygen in stem cell biology: a critical component of the stem cell niche. Cell Stem Cell, 2010, 7(2): 150-161.
9.	Hawkins KE, Sharp TV, McKay TR. The role of hypoxia in stem cell potency and differentiation. Regen Med, 2013, 8(6): 771-782.
10.	王麗, 張會峰, 袁慧娟, 等. 大鼠骨髓內皮祖細胞的分離培養與鑒定. 中國組織工程研究, 2012, 16(10): 1733-1736.
11.	湯文燕, 欒佐. 內皮祖細胞的生物學特性及其臨床應用前景. 中國生物工程雜志, 2016, 36(10): 86-93.
12.	李詠雪, 陸曉, 勵建安. 循環內皮祖細胞與冠心病關系的Meta分析. 中國動脈硬化雜志, 2012, 20(7): 655-660.
13.	李珊姍, 楊鏞. Galectin-3 對外周血內皮祖細胞源性血管內皮細胞增殖能力的影響. 中國普外基礎與臨床雜志, 2012, 19(7): 718-721.
14.	Liao S, Luo C, Cao B, et al. Endothelial progenitor cells for ischemic stroke: update on basic research and application. Stem Cells Int, 2017, 2017: 2193432.
15.	饒璇, 李元建. 內皮細胞損傷與修復的研究進展. 中國動脈硬化雜志, 2017, 25(5): 531-535.
16.	周武. 內皮祖細胞的生物學性狀及其治療作用的研究進展. 東南大學學報(醫學版), 2014, 33(6): 783-787.
17.	Morrone D, Felice F, Scatena C, et al. Role of circulating endothelial progenitor cells in the reparative mechanisms of stable ischemic myocardium. Int J Cardiol, 2017. pii: S0167-5273(16)32271-9. doi: 10.1016/j.ijcard.2017.05.070.[Epub ahead of print] .
18.	Kawamoto A, Katayama M, Handa N, et al. Intramuscular transplantation of G-CSF-mobilized CD34⁺ cells in patients with critical limb ischemia: a phase Ⅰ/Ⅱa, multicenter, single-blinded, dose-escalation clinical trial. Stem Cells, 2009, 27(11): 2857-2864.
19.	Tanaka R, Masuda H, Kato S, et al. Autologous G-CSF-mobilized peripheral blood CD34⁺ cell therapy for diabetic patients with chronic nonhealing ulcer. Cell Transplant, 2014, 23(2): 167-179.
20.	谷涌泉, 郭連瑞, 張建, 等. 自體骨髓干細胞移植治療嚴重下肢缺血 1 例. 中國實用外科雜志, 2003, 23(11): 670.
21.	谷涌泉, 張建, 齊立行, 等. 自體骨髓干細胞和外周血干細胞移植治療下肢缺血的對比研究. 中國修復重建外科雜志, 2007, 21(7): 675-678.
22.	谷涌泉, 佟鑄, 郭連瑞. 干細胞移植治療下肢重度缺血. 中華普通外科學文獻(電子版), 2015, 9(1): 8-10.
23.	Hou J, Wang L, Long H, et al. Hypoxia preconditioning promotes cardiac stem cell survival and cardiogenic differentiation in vitro involving activation of the HIF-1α/apelin/APJ axis. Stem Cell Res Ther, 2017, 8(1): 215.
24.	Yu Y, Yin Y, Wu RX, et al. Hypoxia and low-dose inflammatory stimulus synergistically enhance bone marrow mesenchymal stem cell migration. Cell Prolif, 2017, 50(1). doi: 10.1111/cpr.12309.
25.	Das SK, Yuan YF, Li MQ. An overview on current issues and challenges of endothelial progenitor cell-based neovascularization in patients with diabetic foot ulcer. Cell Reprogram, 2017, 19(2): 75-87.

1. Asahara T, Murohara T, Sullivan A, et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science, 1997, 275(5302): 964-967.
2. 白潔, 孟軍, 蔡澤民, 等. 高脂蛋白(a)的冠心病患者內皮祖細胞功能受損. 中國動脈硬化雜志, 2015, 23(4): 384-388.
3. Yu CG, Zhang N, Yuan SS, et al. Endothelial progenitor cells in diabetic microvascular complications: friends or foes. Stem Cells Int, 2016, 2016:1803989. doi: 10.1155/2016/1803989. Epub 2016 May 29.
4. Kang H, Ma X, Liu J, et al. High glucose-induced endothelial progenitor cell dysfunction. Diab Vasc Dis Res, 2017, 14(5): 381-394.
5. Li TB, Zhang YZ, Liu WQ, et al. Correlation between NADPH oxidase-mediated oxidative stress and dysfunction of endothelial progenitor cell in hyperlipidemic patients. Korean J Intern Med, 2017, doi: 10.3904/kjim.2016.140.[Epub ahead of print].
6. King TF, McDermott JH. Endothelial progenitor cells and cardiovascular disease. J Stem Cells, 2014, 9(2): 93-106.
7. 徐竹, 諸葛啟釧, 黃李潔. 干細胞3D支架的研究進展. 中國生物工程雜志, 2017, 37(9): 112-117.
8. Mohyeldin A, Garzón-Muvdi T, Qui?ones-Hinojosa A. Oxygen in stem cell biology: a critical component of the stem cell niche. Cell Stem Cell, 2010, 7(2): 150-161.
9. Hawkins KE, Sharp TV, McKay TR. The role of hypoxia in stem cell potency and differentiation. Regen Med, 2013, 8(6): 771-782.
10. 王麗, 張會峰, 袁慧娟, 等. 大鼠骨髓內皮祖細胞的分離培養與鑒定. 中國組織工程研究, 2012, 16(10): 1733-1736.
11. 湯文燕, 欒佐. 內皮祖細胞的生物學特性及其臨床應用前景. 中國生物工程雜志, 2016, 36(10): 86-93.
12. 李詠雪, 陸曉, 勵建安. 循環內皮祖細胞與冠心病關系的Meta分析. 中國動脈硬化雜志, 2012, 20(7): 655-660.
13. 李珊姍, 楊鏞. Galectin-3 對外周血內皮祖細胞源性血管內皮細胞增殖能力的影響. 中國普外基礎與臨床雜志, 2012, 19(7): 718-721.
14. Liao S, Luo C, Cao B, et al. Endothelial progenitor cells for ischemic stroke: update on basic research and application. Stem Cells Int, 2017, 2017: 2193432.
15. 饒璇, 李元建. 內皮細胞損傷與修復的研究進展. 中國動脈硬化雜志, 2017, 25(5): 531-535.
16. 周武. 內皮祖細胞的生物學性狀及其治療作用的研究進展. 東南大學學報(醫學版), 2014, 33(6): 783-787.
17. Morrone D, Felice F, Scatena C, et al. Role of circulating endothelial progenitor cells in the reparative mechanisms of stable ischemic myocardium. Int J Cardiol, 2017. pii: S0167-5273(16)32271-9. doi: 10.1016/j.ijcard.2017.05.070.[Epub ahead of print] .
18. Kawamoto A, Katayama M, Handa N, et al. Intramuscular transplantation of G-CSF-mobilized CD34⁺ cells in patients with critical limb ischemia: a phase Ⅰ/Ⅱa, multicenter, single-blinded, dose-escalation clinical trial. Stem Cells, 2009, 27(11): 2857-2864.
19. Tanaka R, Masuda H, Kato S, et al. Autologous G-CSF-mobilized peripheral blood CD34⁺ cell therapy for diabetic patients with chronic nonhealing ulcer. Cell Transplant, 2014, 23(2): 167-179.
20. 谷涌泉, 郭連瑞, 張建, 等. 自體骨髓干細胞移植治療嚴重下肢缺血 1 例. 中國實用外科雜志, 2003, 23(11): 670.
21. 谷涌泉, 張建, 齊立行, 等. 自體骨髓干細胞和外周血干細胞移植治療下肢缺血的對比研究. 中國修復重建外科雜志, 2007, 21(7): 675-678.
22. 谷涌泉, 佟鑄, 郭連瑞. 干細胞移植治療下肢重度缺血. 中華普通外科學文獻(電子版), 2015, 9(1): 8-10.
23. Hou J, Wang L, Long H, et al. Hypoxia preconditioning promotes cardiac stem cell survival and cardiogenic differentiation in vitro involving activation of the HIF-1α/apelin/APJ axis. Stem Cell Res Ther, 2017, 8(1): 215.
24. Yu Y, Yin Y, Wu RX, et al. Hypoxia and low-dose inflammatory stimulus synergistically enhance bone marrow mesenchymal stem cell migration. Cell Prolif, 2017, 50(1). doi: 10.1111/cpr.12309.
25. Das SK, Yuan YF, Li MQ. An overview on current issues and challenges of endothelial progenitor cell-based neovascularization in patients with diabetic foot ulcer. Cell Reprogram, 2017, 19(2): 75-87.

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Hypoxia condition can enhance proliferation, adhesion, migration, and viability abilities of bone morrow-derived endothelial progenitor cells

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