Effects of hedysarum polysaccharides-1 on oxidative stress in lung adenocarcinoma A549 cells_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

WANG Xiaojun ,  LIU Hua

Department of Respiratory Medicine, The People's Hospital of Gansu Province, Lanzhou, Gansu 730000, P.R.China;

Corresponding?author：

LIU Hua, Email: 13919965016@163.com

Keywords：

Hedysarum polysaccharides-1; A549 cells; Proliferation; Apoptosis; Oxidative stress

DOI：

10.7507/1671-6205.201608025

Video：

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Objective To evaluate the expression of reactive oxygen species (ROS), glutataione (GSH), total superoxide dismutase (T-SOD), total antioxidation capacity (T-AOC), thioredoxin reductase (TrxR) under the intervention of hedysarum polysaccharides-1 (HPS-1) in A549 cells. Methods After treated by HPS-1 in different doses (50 mg/L, 100 mg/L, 200 mg/L, respectively), the viability of cell lines was detected by MTT method under microscope. The apoptosis of cell lines was detected by flow cytometry (FCM). The expressions of ROS, GSH, T-SOD, T-AOC, and TrxR in cell supernatant were measured by chemiluminescence method. Results Determined by MTT/FCM/ELISA, the results showed that different doses of HPS-1 could inhibit the proliferation and promote the apoptosis of A549 cells (allP<0.05). The expression levels of GSH, T-SOD, T-AOC, and TrxR were significantly decreased (allP<0.05) and the expression levels of ROS and MDA were significantly increased (allP<0.05) in a concentration-dependent manner in A549 cells treated with HPS-1, and these effects were significantly weakened in A549 cells with time extending (allP<0.05). Conclusion HPS-1 has a markedly effect on inhibiting cellular proliferation and inducing cellular apoptosis of lung adenocarcinoma A549 cells, which may be associated with the change of oxidation/antioxidant.

Citation： WANG Xiaojun, LIU Hua. Effects of hedysarum polysaccharides-1 on oxidative stress in lung adenocarcinoma A549 cells. Chinese Journal of Respiratory and Critical Care Medicine, 2017, 16(2): 127-131. doi: 10.7507/1671-6205.201608025 Copy

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11.	姚寶泰, 趙健雄, 王學習, 等. 紅芪總多糖體內抗腫瘤的實驗研究. 中華中醫藥雜志, 2008, 23(7): 627-629.
12.	李世剛, 張永琦, 趙健雄, 等. 紅芪多糖 HPS-3 體外誘導人胃癌 MGC-803 細胞凋亡的研究. 中藥藥理與臨床, 2007, 23(3): 103-105.
13.	顧達民, 陳宇 ,張小寶, 等. 氯胺酮對脂多糖誘導的大鼠肺泡巨噬細胞氧化應激的影響. 實用臨床醫藥雜志, 2008, 12(13): 1-2.
14.	惠和平, 封士蘭, 胡芳弟, 等. 紅芪多糖的體外抗氧化活性研究. 安徽農業科學, 2010, 38(8): 4056-4057.
15.	劉靜, 鄧文娟, 魏素虹, 等, 紅芪多糖對高糖條件下人臍靜脈內皮細胞功能的影響. 中華糖尿病雜志, 2013, 5(9): 560-564.

1. ?Stanzani F, Paisani DM, Oliveira A, et al. Morbidity, and categorization of the risk of perioperative complications in lung cancer patients. Bras Pneumol, 2014, 40(1): 21-29.
2. Faber AC, Ebi H, Costa C, et al. Apoptosis in targeted therapy responses: the role of BIM. Adv Pharmacol, 2012, 65: 519-542.
3. Pore MM, Hiltermann TJ, Kruyt FA. Targeting apoptosis pathways in lung cancer. Cancer Lett, 2013, 332(2): 359-368.
4. Lima RT, Busacca S, Almeida GM, et al. MicroRNA regulation of core apoptosis pathways in cancer. Eur J Cancer, 2011, 47(2): 163-174.
5. Jia LT, Chen SY, Yang AG. Cancer gene therapy targeting cellular apoptosis machinery. Cancer Treat Rev, 2012, 38(7): 868-876.
6. 楊扶德, 羅文蓉, 林麗, 等. 甘肅紅芪質量標準研究. 中國藥房, 2008, 19(18): 1391-1393.
7. 董玉梅, 王小虎. 紅芪多糖抗腫瘤作用的研究進展. 腫瘤預防與治療, 2012, 25(3): 194-197.
8. 劉華, 閆立萍, 王小軍. 紅芪多糖 1 聯合順鉑對肺癌 A549 細胞凋亡的協同作用及其機制探討. 腫瘤, 2013, 33(11): 980-984.
9. 王亞麗, 孫超, 張昕, 等. MST-312 對人肝癌 HepG-2 細胞輻射敏感性影響研究. 中華腫瘤防治雜志, 2015, 22(1): 1-6.
10. 田河, 邸彥橙, 宋靜, 等. 紅芪多糖對膀胱癌大鼠抗腫瘤作用的實驗研究. 國外醫藥抗生素分冊, 2015, 36(1): 27-28.
11. 姚寶泰, 趙健雄, 王學習, 等. 紅芪總多糖體內抗腫瘤的實驗研究. 中華中醫藥雜志, 2008, 23(7): 627-629.
12. 李世剛, 張永琦, 趙健雄, 等. 紅芪多糖 HPS-3 體外誘導人胃癌 MGC-803 細胞凋亡的研究. 中藥藥理與臨床, 2007, 23(3): 103-105.
13. 顧達民, 陳宇 ,張小寶, 等. 氯胺酮對脂多糖誘導的大鼠肺泡巨噬細胞氧化應激的影響. 實用臨床醫藥雜志, 2008, 12(13): 1-2.
14. 惠和平, 封士蘭, 胡芳弟, 等. 紅芪多糖的體外抗氧化活性研究. 安徽農業科學, 2010, 38(8): 4056-4057.
15. 劉靜, 鄧文娟, 魏素虹, 等, 紅芪多糖對高糖條件下人臍靜脈內皮細胞功能的影響. 中華糖尿病雜志, 2013, 5(9): 560-564.

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Effects of hedysarum polysaccharides-1 on oxidative stress in lung adenocarcinoma A549 cells

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