Screening of Genes Differentially Expressed in A549 Cells Transfected with FOXO1 Using DNA Microarray_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

SunQingfeng ^1,2 , LiLi ² , ZhangAnbing ² ,  HuangWenjie ^1,2

1. Postgraduate Institute of Guangdong Pharmaceutical University, Guangzhou, Guangdong, 510006, China;
2. ;

Corresponding?author：

HuangWenjie, Email: huangyelu1029@vip.163.com

Keywords：

Forkhead box protein O1; DNA microarray; A549 cells; Acute lung injury

DOI：

10.7507/1671-6205.2016087

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To study the differences in gene expression in A549 cells transfected with Forkhead box protein O1(FOXO1),and provide clues to further exploring the mechanism of FOXO1 in acute lung injury. Methods After using TNF-α to stimulate A549 cells,the eukaryotic expression vector GV230-FOXO1 was transfected into A549 cells by using lipofectamine transfection reagent.The RNA was isolated and differentially expressed genes were screened with high-throughout DNA microarray. Results The eukaryotic expression vector GV230-FOXO1 was successfully constructed and verified.High quality mRNA was isolated and prepared for microarray screening,which passed RNA quality control.The DNA microarray data indicated that 317 genes were up-regulated and 237 genes were down-regulated in A549 cells transfected with FOXO1.The function of these differentially expressed genes involved in many aspects,such as proliferation,apoptosis and differentiation. Conclusions Differentially expressed genes in A549 cells transfected with FOXO1 can be successfully screened by using DNA microarray.FOXO1 may influence the progression of the disease by changing the level of cell proliferation,apoptosis and differentiation in acute lung injury.

Citation： SunQingfeng, LiLi, ZhangAnbing, HuangWenjie. Screening of Genes Differentially Expressed in A549 Cells Transfected with FOXO1 Using DNA Microarray. Chinese Journal of Respiratory and Critical Care Medicine, 2016, 15(4): 368-373. doi: 10.7507/1671-6205.2016087 Copy

1.	宋振舉, 白春學.我國急性肺損傷/急性呼吸窘迫綜合癥臨床和實驗研究進展.內科理論與實踐,2010,5:496-99.
2.	Lin L,Hron JD,Peng SL.Regulation of NF-kappaB,Th activation,and autoinflammation by the forkhead transcription factor Foxo3a.Immunity,2004,21:203-213.
3.	Yang J,Zong CS,Xia W,et al.ERK promotes tumorigenesis by inhibiting FOXO3a via MDM2-mediated degradation.Nature Cell Biol,2008,10:138-148.
4.	Hu MC,Lee D,Xia W,et al.IκB kinase promotes tumorigenesis through inhibition of forkhead FOXO3a.Cell,2004,117:225-237.
5.	Urbich C,Knau A,Fichtlscherer S,et al.FOXO-dependent expression of the proapoptotic protein Bim:pivotal role for apoptosis signaling in endothelial progenitor cells.FASEB J,2005,19:974-976.
6.	朱云喜, 梁繼河,李琦等. TNF-α和IL-8在兔創傷性急性肺損傷中的意義.免疫學雜志,1997,13:149-151.
7.	陳業民, 黃文杰,李勝利,等.重癥肺炎大鼠干擾素-γ、白細胞介素-6和腫瘤壞死因子-α含量變化.中國病理生理雜志,2007,23:492-94.
8.	Weifeng Y,Li L,Yujie H,et al.Inhibition of acute lung injury by TNFR-Fc through regulation of an inflammation-oxidative stress pathway.PLoS One,2016,11:e0151672.
9.	黃建華, 李理,邱嫻,等.肺泡Ⅱ型上皮細胞A549對TNF-α和LPS的刺激效果研究.免疫學雜志,2013,29:211-215.
10.	Wang XW,Yu Y,Gu L.Dehydroabietic acid reverses TNF-α-induced the activation of FOXO1 and suppression of TGF-β1/Smad signaling in human adult dermal fibroblasts.Int J Clin Exp Pathol,2014,7:8616-8626.
11.	李理, 趙立媛,袁偉鋒,等.FOXO1熒光真核表達載體構建及其對TNF-α介導的A519細胞促凋亡蛋白Bim表達的影響.中華醫學會呼吸病學年會-2015第十六次全國呼吸病學學術會議論文,No.4076.
12.	Xie L,Ritz O,Leith?user F,et al.FOXO1 downregulation contributes to the oncogenic program of primary mediastinal B-cell lymphoma.Oncotarget,2014,5:5392-5402.
13.	Jacquot C,Rousseau B,Carbonnelle D,et al.Cucurbitacin-D-induced CDK1 mRNA up-regulation causes proliferation arrest of a non-small cell lung carcinoma cell line (NSCLC-N6).Anticancer Res,2014,34:4797-806.
14.	de Cárcer G,Escobar B,Higuero AM,et al.Plk5,a polo box domain-only protein with specific roles in neuron differentiation and glioblastoma suppression.Mol Cell Biol,2011,31:1225-1239.
15.	鄔皓晨, 陳勇軍,徐易塵,等.Caspase-1在炎癥及程序性細胞死亡過程中的作用.中國細胞生物學學報,2011,33:182-189.
16.	Bebenek M,Du As'G D,Ko Az'G lak J.Prognostic value of the Fas/Fas ligand system in breast cancer.Contemp Oncol (Pozn),2013,17:120-122.
17.	Yang E,Zha J,Jockel J,et al.Bad,a heterodimeric partner for Bcl-XL and Bcl-2,displaces Bax and promotes cell death.Cell,1995,80:285-291.
18.	Abraham SC,Nubukawa B,Giardicllo FM,et al.Fundic gland polyps in familial adenomatous polyposis neoplasms with frequent somatic adenomatous polyposis coli gene alterations.Am J Pathol,2000,157:747-754.
19.	Wilkaniec A,Czapski GA,Adamczyk A.Cdk5 at crossroads of protein oligomerization in neurodegenerative diseases:facts and hypotheses.J Neurochem,2015,136:222-233.

1. 宋振舉, 白春學.我國急性肺損傷/急性呼吸窘迫綜合癥臨床和實驗研究進展.內科理論與實踐,2010,5:496-99.
2. Lin L,Hron JD,Peng SL.Regulation of NF-kappaB,Th activation,and autoinflammation by the forkhead transcription factor Foxo3a.Immunity,2004,21:203-213.
3. Yang J,Zong CS,Xia W,et al.ERK promotes tumorigenesis by inhibiting FOXO3a via MDM2-mediated degradation.Nature Cell Biol,2008,10:138-148.
4. Hu MC,Lee D,Xia W,et al.IκB kinase promotes tumorigenesis through inhibition of forkhead FOXO3a.Cell,2004,117:225-237.
5. Urbich C,Knau A,Fichtlscherer S,et al.FOXO-dependent expression of the proapoptotic protein Bim:pivotal role for apoptosis signaling in endothelial progenitor cells.FASEB J,2005,19:974-976.
6. 朱云喜, 梁繼河,李琦等. TNF-α和IL-8在兔創傷性急性肺損傷中的意義.免疫學雜志,1997,13:149-151.
7. 陳業民, 黃文杰,李勝利,等.重癥肺炎大鼠干擾素-γ、白細胞介素-6和腫瘤壞死因子-α含量變化.中國病理生理雜志,2007,23:492-94.
8. Weifeng Y,Li L,Yujie H,et al.Inhibition of acute lung injury by TNFR-Fc through regulation of an inflammation-oxidative stress pathway.PLoS One,2016,11:e0151672.
9. 黃建華, 李理,邱嫻,等.肺泡Ⅱ型上皮細胞A549對TNF-α和LPS的刺激效果研究.免疫學雜志,2013,29:211-215.
10. Wang XW,Yu Y,Gu L.Dehydroabietic acid reverses TNF-α-induced the activation of FOXO1 and suppression of TGF-β1/Smad signaling in human adult dermal fibroblasts.Int J Clin Exp Pathol,2014,7:8616-8626.
11. 李理, 趙立媛,袁偉鋒,等.FOXO1熒光真核表達載體構建及其對TNF-α介導的A519細胞促凋亡蛋白Bim表達的影響.中華醫學會呼吸病學年會-2015第十六次全國呼吸病學學術會議論文,No.4076.
12. Xie L,Ritz O,Leith?user F,et al.FOXO1 downregulation contributes to the oncogenic program of primary mediastinal B-cell lymphoma.Oncotarget,2014,5:5392-5402.
13. Jacquot C,Rousseau B,Carbonnelle D,et al.Cucurbitacin-D-induced CDK1 mRNA up-regulation causes proliferation arrest of a non-small cell lung carcinoma cell line (NSCLC-N6).Anticancer Res,2014,34:4797-806.
14. de Cárcer G,Escobar B,Higuero AM,et al.Plk5,a polo box domain-only protein with specific roles in neuron differentiation and glioblastoma suppression.Mol Cell Biol,2011,31:1225-1239.
15. 鄔皓晨, 陳勇軍,徐易塵,等.Caspase-1在炎癥及程序性細胞死亡過程中的作用.中國細胞生物學學報,2011,33:182-189.
16. Bebenek M,Du As'G D,Ko Az'G lak J.Prognostic value of the Fas/Fas ligand system in breast cancer.Contemp Oncol (Pozn),2013,17:120-122.
17. Yang E,Zha J,Jockel J,et al.Bad,a heterodimeric partner for Bcl-XL and Bcl-2,displaces Bax and promotes cell death.Cell,1995,80:285-291.
18. Abraham SC,Nubukawa B,Giardicllo FM,et al.Fundic gland polyps in familial adenomatous polyposis neoplasms with frequent somatic adenomatous polyposis coli gene alterations.Am J Pathol,2000,157:747-754.
19. Wilkaniec A,Czapski GA,Adamczyk A.Cdk5 at crossroads of protein oligomerization in neurodegenerative diseases:facts and hypotheses.J Neurochem,2015,136:222-233.

Chinese Journal of Respiratory and Critical Care Medicine

Screening of Genes Differentially Expressed in A549 Cells Transfected with FOXO1 Using DNA Microarray

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