Protection of resveratrol on seawater-drowning-induced lung injury in rats_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

HUANG Yansheng , WEN Wen ,  LAI Guoxiang , PAN Jianguang , SU Shizhen

Department of Respiratory and Critical Care Medicine, No. 900 Hospital of The Chinese People's Liberation Army Joint Logistic Support Force, Fuzhou, Fujian 350000, P. R. China;

Corresponding?author：

LAI Guoxiang, Email: laiguoxiang2007@163.com

Keywords：

Pulmonary edema; Seawater drowning; Resveratrol; Apoptosis

DOI：

10.7507/1671-6205.202012019

Video：

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Objective To improve the seawater-drowning-induced lung injury model in rats, and investigate the protective effect of resveratrol against seawater-drowning-induced lung injury and its mechanism.Methods A total of 112 SD healthy rats were randomly assigned into 5 groups: a control group (Group C, n=8), a seawater drowning group (Group S, n=32), a resveratrol prophylactic treatment group (Group S+R, n=32), a resveratrol group (Group R, n=8), and an endotracheal intubation group (Group E, n=32). A modified endotracheal intubation model was developed, and endotracheal intubation was used instead of tracheotomy. Blood gas analysis was performed on the abdominal aorta at each time point, then the rats were sacrificed to obtain their lungs. Lung wet-to-dry ratio (W/D), malondialdehyde (MDA), superoxide dismutase (SOD), myeloperoxidase (MPO) and cysteinyl aspartate specific proteinase (Caspase-3) were measured by enzyme linked immunosorbent assay. The histological sections of rat lungs were stained with haematoxylin-eosin. Groups S+R and R were pretreated with resveratrol (50 mg/kg) through intragastric administration for 3 days; then models were established and the rats were sacrificed 24 hours after the last intragastric administration.Results After seawater perfusion, arterial oxygen pressure decreased and arterial carbon dioxide pressure increased in blood gas analysis of rats, MDA content increased, MPO and SOD activity decreased, caspase-3 content and W/D ratio increased, as well as lung tissue pathological damage. The resveratrol pretreatment group showed the same change trend, but the damage degree was relatively light.Conclusions Seawater perfusion can induce respiratory failure, pulmonary edema and hemorrhage in rats. Lung tissue apoptosis may occur when seawater submergence causes lung injury. Resveratrol pretreatment can ameliorate hypoxia and pulmonary edema in rats.

Citation： HUANG Yansheng, WEN Wen, LAI Guoxiang, PAN Jianguang, SU Shizhen. Protection of resveratrol on seawater-drowning-induced lung injury in rats. Chinese Journal of Respiratory and Critical Care Medicine, 2021, 20(8): 570-576. doi: 10.7507/1671-6205.202012019 Copy

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15.	Zhang ML, Gao YH, Zhao WG, et al. ACE-2/ANG1-7 ameliorates ER stress-induced apoptosis in seawater aspiration-induced acute lung injury. Am J Physiol Lung Cell Mol Physiol, 2018, 315(6): L1015-L1027.
16.	顧興, 金發光, 傅恩清, 等. 海水淹溺兔肺泡巨噬細胞 TNF-α mRNA 的動態表達及地塞米松干預作用. 現代生物醫學進展, 2007, 7(11): 1631-1634.
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1. 梁源, 金發光. PS 在海水淹溺肺水腫和海水型呼吸窘迫綜合征中的治療作用. 國際呼吸雜志, 2007, 27(18): 1390-1392.
2. WHO. Global report on drowning: preventing a leading killer. Geneva: World Health Organization, 2016.
3. Li CC, Bo LY, Li PC, et al. Losartan, a selective antagonist of AT1 receptor, attenuates seawater inhalation induced lung injury via modulating JAK2/STATs and apoptosis in rat. Pulm Pharmacol Ther, 2017, 45: 69-79.
4. Liu ZY, Xi RG, Zhang ZR, et al. 4-hydroxyphenylacetic acid attenuated inflammation and edema via suppressing HIF-1α in seawater aspiration-induced lung injury in rats. Int J Mol Sci, 2014, 15(7): 12861-12884.
5. Ji MH, Tong JH, Tan YH, et al. Erythropoietin pretreatment attenuates seawater aspiration-induced acute lung injury in rats. Inflammation, 2016, 39(1): 447-456.
6. 張喜云. 虎杖的化學成分、藥理作用與提取分離. 天津藥學, 1999, 11(3): 13.
7. 趙克森. 白藜蘆醇的生物學特性和效應. 中國病理生理雜志, 2012, 28(9): 1709-1711, 1717.
8. Bagchi D. Resveratrol and human health. 1st ed. Los Angeles: Keats Pub, 2000.
9. Aggarwal BB, Bhardwaj A, Aggarwal RS, et al. Role of resveratrol in prevention and therapy of cancer: preclinical and clinical studies. Anticancer Res, 2004, 24(5A): 2783-2840.
10. 趙克森. 虎杖苷抗休克的機理研究. 微循環學雜志, 2006, 16(2): 1-5.
11. Zhao KS, Jin CH, Huang XL, et al. The mechanism of Polydatin in shock treatment. Clin Hemorheol Microcirc, 2003, 29(3-4): 211-217.
12. 余丹. 氨溴索聯合地塞米松治療海水淹溺性肺損傷的實驗研究[D]. 福建醫科大學, 2017.
13. 馬李杰, 趙詣林, 劉雪英, 等. 乙酰化白藜蘆醇保護海水淹溺型急性肺損傷的機制研究. 中國急救醫學, 2013, 33(1): 74-76, 后插4.
14. 李聰聰. 蛋白激酶 SPAK 在海水吸入型肺損傷中的作用及相關機制的研究[D]. 第四軍醫大學, 2014: 1-59.
15. Zhang ML, Gao YH, Zhao WG, et al. ACE-2/ANG1-7 ameliorates ER stress-induced apoptosis in seawater aspiration-induced acute lung injury. Am J Physiol Lung Cell Mol Physiol, 2018, 315(6): L1015-L1027.
16. 顧興, 金發光, 傅恩清, 等. 海水淹溺兔肺泡巨噬細胞 TNF-α mRNA 的動態表達及地塞米松干預作用. 現代生物醫學進展, 2007, 7(11): 1631-1634.
17. Lv HM, Liu QM, Wen ZM, et al. Xanthohumol ameliorates lipopolysaccharide (LPS)-induced acute lung injury via induction of AMPK/GSK3β-Nrf2 signal axis. Redox Biol, 2017, 12(2): 311-324.
18. 姜凱麗, 張錫剛. 活性氧在急性肺損傷/急性呼吸窘迫綜合征中的作用. 中華損傷與修復雜志(電子版), 2010, 5(2): 249-253.
19. 馬李杰. 乙酰化白藜蘆醇對大鼠海水吸入型肺損傷的保護作用研究[D]. 第四軍醫大學, 2013: 1-60.

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Protection of resveratrol on seawater-drowning-induced lung injury in rats

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