PCR-Single-Strand Conformational Polymorphism Method for Detecting rpoB Gene of Rifampin-Resistant Mycobacterium Tuberculosis: A Meta-Analysis_Chinese Journal of Evidence-Based Medicine

Authors：

 YULu , MENGCun-ren ,  ZHANGZhao-xia

Department of Laboratory Medicine, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China;

Corresponding?author：

ZHANGZhao-xia, Email: 285715300@qq.com

Keywords：

Mycobacterium tuberculosis; Rifampin; rpoB; Polymerase chain reaction-single strand conformation polymorphism; Meta-analysis

DOI：

10.7507/1672-2531.20140119

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To systematically review the diagnostic value of PCR-single-strand conformational polymorphism (PCR-SSCP) method for detecting rpoB gene mutation of rifampin-resistant mycobacterium tuberculosis. Methods Such databases as PubMed, Web of Science, The Cochrane Library (Issue 2, 2014), CBM, VIP and WanFang Data were electronically and comprehensively searched for relevant studies on the diagnostic value of PCR-SSCP method for detecting rpoB gene mutation of rifampin-resistant mycobacterium tuberculosis from inception to January 1^st, 2014. Literature screening according to the inclusion and exclusion criteria, data extraction and methodological quality assessment were completed by two reviewers independently. Meta-analysis was then conducted using Meta-Disc 1.4 and Stata 12.0. Results A total of 10 studies were included involving 1 299 cases. The results of meta-analysis showed SEN=0.92 (95%CI 0.90 to 0.94, P=0.019 3), SPE=0.97 (95%CI 0.95 to 0.98, P < 0.000 1), +LR=23.68 (95%CI 8.71 to 64.37, P < 0.000 1), -LR=0.10 (95%CI 0.06 to 0.15, P=0.023 1), DOR=257.16 (95%CI 96.82 to 683.02, P=0.020 0), and SROC area under the curve (AUC) was 0.971 5, and Q* was 0.922 3. The results of sensitivity analysis (after removing studies with sample size less than 100, Chinese studies and QUADAS more than 10 studies) showed that, the results were stable with reliable conclusion. Conclusion PCR-SSCP method has a fairly high value in the diagnosis of rpoB gene mutation of rifampinresistant mycobacterium tuberculosis.

Citation： YULu, MENGCun-ren, ZHANGZhao-xia. PCR-Single-Strand Conformational Polymorphism Method for Detecting rpoB Gene of Rifampin-Resistant Mycobacterium Tuberculosis: A Meta-Analysis. Chinese Journal of Evidence-Based Medicine, 2014, 14(6): 698-706. doi: 10.7507/1672-2531.20140119 Copy

1.	?Zumla A, Abubakar I, Raviglione M, et al. Drug-resistant tuberculosis-current dilemmas, unanswered questions, challenges, and priority needs. J Infect Dis, 2012, 205(2):228-240.
2.	孟繁榮, 劉志輝, 謝貝, 等.我國利福平敏感結核桿菌rpoB基因利福平耐藥定區序列循證分析.實用醫學雜志, 2009, 29(21):3601-3604.
3.	Miller LP, Crawford JT, Shinnick TM. The rpoB gene of Mycobacterium tuberculosis. Ntimicrob Agents Ch, 1994, 38(4):805-811.
4.	Palomino J C. Nonconventional and new methods in the diagnosis of tuberculosis:feasibility and applicability in the field. Eur Respir J, 2005, 26(2):339-350.
5.	Palomino JC. Newer diagnostics for tuberculosis and multi-drug resistant tuberculosis. Curr Opin Pulm Med, 2006, 12(3):172-178.
6.	Isfahani BN, Tavakoli A, Salehi M, et al. Detection of rifampin resistance patterns in Mycobacterium tuberculosis strains isolated in Iran by polymerase chain reaction-single-strand conformation polymorphism and direct sequencing methods. Mem Inst Oswaldo Cruz, Rio de Janeiro, 2006, 101(6):597-602.
7.	Scarpellini P, Braglia S, Brambilla AM, et al. Detection of rifampin resistance by single-strand conformation polymorphism analysis of cerebrospinal fluid of patients with tuberculosis of the central nervous system. J Clin Microbiol, 1997, 35(11):2802-2806.
8.	汪保國, 陳思東, 張勝, 等.結核分支桿菌rpoB基因突變與對利福平耐藥的關系.實用醫學雜志, 2009, 25(7):1022-1024.
9.	Whiting P, Rutjes AW, Reitsma IB, 等. QUADAS的制定:用于系統評價中評價診斷性研究質量的工具(譯文).中國循證醫學雜志, 2007, 7(4):296-306.
10.	Whiting P, Weswood ME, Rutjes AW, 等. QUADAS評價:一種用于診斷性研究的質量評價工具(修訂版)(譯文).中國循證醫學雜志, 2007, 7(7):531-536.
11.	張天嵩, 鐘文昭. Meta Disc軟件在診斷試驗Meta分析中的應用.循證醫學, 2008, 8(2):97-108.
12.	楊柳, 蘇明權, 程曉東, 等. PCR-SSCP法檢測結核分枝桿菌耐藥性.第四軍醫大學學報, 2004, 24(23):2142-214.
13.	鐘敏, 溫博海.檢測結核分枝桿菌rpoB基因突變的研究.中華檢驗醫學雜志, 2002, 25(1):38-41.
14.	褚美芬, 嚴杰, 鐘雅文, 等.快速檢測結核分枝桿菌異煙肼和利福平耐藥相關基因mPCR-SSCP建立及應用.中華微生物學和免疫學雜志, 2012, 32(7):655-660.
15.	Cheng X, Zhang J, Yang L, et al. A new Multi-PCR-SSCP assay for simultaneous detection of isoniazid and rifampin resistance in mycobacterium tuberculosis. J Microbiol Meth, 2007, 70:301-305.
16.	Telenti A, Honore N, Bernasconi C, et al. Genotypic assessment of isoniazid and rifampin resistance in mycobacterium tuberculosis: a blind study at reference laboratory level. J Clin Microbiol, 1997, 35(3):719-723.
17.	Sheng J, Li J, Sheng G, et al. Characterization of rpoB mutations associated with rifampin resistance in Mycobacterium tuberculosis from eastern China. J Appl Microbiol, 2008, 105(3):904-911.
18.	Negi SS, Rai A, Gupta S, et al. Characterization of rpoB gene for detection of rifampicin drug resistance by SSCP and sequence analysis. Int J Infect Dis, 2008, 12:e318.
19.	Mani C, Selvakumar N, Kumar V, et al. Comparison of DNA sequencing, PCR-SSCP and PhaB assays with indirect sensitivity testing for detection of rifampicin resistance in mycobacterium tuberculosis. Int J Tuberc Lung D, 2003, 7(7):652-659.
20.	Kim BJ, Lee KH, Yun YJ, et al. Simultaneous identification of rifampin-resistant Mycobacterium tuberculosis and nontuberculous mycobacteria by polymerase chain reaction-single strand conformation polymorphism and sequence analysis of the RNA polymerase gene (rpoB). J Microbiol Meth, 2004, 58(1):111-118.
21.	Wu X, Zhang J, Zhuang Y, et al. Molecular mechanisms of drug resistance in mycobacterium tuberculosis clinical isolates. CMJ, 1999, 112(6):524-528.
22.	Devillé WL, Buntinx F, Bouter LM, et al. Conducting systematic reviews of diagnostic studies:didactic guidelines. BMC Medical Research Methodology, 2002, 2(1):1-13.
23.	柳清云, 孫剛, 高謙.結核分枝桿菌(MTB)異質性耐藥研究進展.復旦學報(醫學版), 2013, 40(1):1-4.
24.	Bossuyt PMM, Reitsma JB, Bruns DE, et al. The STARD statement for reporting studies of diagnostic accuracy:Explanation and elaboration. Clin Chem, 2003, 49(1):7-18.
25.	Tackmann R'Schuetz G, HammB, et al. Quality of the reporting of diagnostic accuracy studies:STARD (Standards for the Reporting of Diagnostic accuracy studies). Rofo, 2010, 182(8):655-659.

1. ?Zumla A, Abubakar I, Raviglione M, et al. Drug-resistant tuberculosis-current dilemmas, unanswered questions, challenges, and priority needs. J Infect Dis, 2012, 205(2):228-240.
2. 孟繁榮, 劉志輝, 謝貝, 等.我國利福平敏感結核桿菌rpoB基因利福平耐藥定區序列循證分析.實用醫學雜志, 2009, 29(21):3601-3604.
3. Miller LP, Crawford JT, Shinnick TM. The rpoB gene of Mycobacterium tuberculosis. Ntimicrob Agents Ch, 1994, 38(4):805-811.
4. Palomino J C. Nonconventional and new methods in the diagnosis of tuberculosis:feasibility and applicability in the field. Eur Respir J, 2005, 26(2):339-350.
5. Palomino JC. Newer diagnostics for tuberculosis and multi-drug resistant tuberculosis. Curr Opin Pulm Med, 2006, 12(3):172-178.
6. Isfahani BN, Tavakoli A, Salehi M, et al. Detection of rifampin resistance patterns in Mycobacterium tuberculosis strains isolated in Iran by polymerase chain reaction-single-strand conformation polymorphism and direct sequencing methods. Mem Inst Oswaldo Cruz, Rio de Janeiro, 2006, 101(6):597-602.
7. Scarpellini P, Braglia S, Brambilla AM, et al. Detection of rifampin resistance by single-strand conformation polymorphism analysis of cerebrospinal fluid of patients with tuberculosis of the central nervous system. J Clin Microbiol, 1997, 35(11):2802-2806.
8. 汪保國, 陳思東, 張勝, 等.結核分支桿菌rpoB基因突變與對利福平耐藥的關系.實用醫學雜志, 2009, 25(7):1022-1024.
9. Whiting P, Rutjes AW, Reitsma IB, 等. QUADAS的制定:用于系統評價中評價診斷性研究質量的工具(譯文).中國循證醫學雜志, 2007, 7(4):296-306.
10. Whiting P, Weswood ME, Rutjes AW, 等. QUADAS評價:一種用于診斷性研究的質量評價工具(修訂版)(譯文).中國循證醫學雜志, 2007, 7(7):531-536.
11. 張天嵩, 鐘文昭. Meta Disc軟件在診斷試驗Meta分析中的應用.循證醫學, 2008, 8(2):97-108.
12. 楊柳, 蘇明權, 程曉東, 等. PCR-SSCP法檢測結核分枝桿菌耐藥性.第四軍醫大學學報, 2004, 24(23):2142-214.
13. 鐘敏, 溫博海.檢測結核分枝桿菌rpoB基因突變的研究.中華檢驗醫學雜志, 2002, 25(1):38-41.
14. 褚美芬, 嚴杰, 鐘雅文, 等.快速檢測結核分枝桿菌異煙肼和利福平耐藥相關基因mPCR-SSCP建立及應用.中華微生物學和免疫學雜志, 2012, 32(7):655-660.
15. Cheng X, Zhang J, Yang L, et al. A new Multi-PCR-SSCP assay for simultaneous detection of isoniazid and rifampin resistance in mycobacterium tuberculosis. J Microbiol Meth, 2007, 70:301-305.
16. Telenti A, Honore N, Bernasconi C, et al. Genotypic assessment of isoniazid and rifampin resistance in mycobacterium tuberculosis: a blind study at reference laboratory level. J Clin Microbiol, 1997, 35(3):719-723.
17. Sheng J, Li J, Sheng G, et al. Characterization of rpoB mutations associated with rifampin resistance in Mycobacterium tuberculosis from eastern China. J Appl Microbiol, 2008, 105(3):904-911.
18. Negi SS, Rai A, Gupta S, et al. Characterization of rpoB gene for detection of rifampicin drug resistance by SSCP and sequence analysis. Int J Infect Dis, 2008, 12:e318.
19. Mani C, Selvakumar N, Kumar V, et al. Comparison of DNA sequencing, PCR-SSCP and PhaB assays with indirect sensitivity testing for detection of rifampicin resistance in mycobacterium tuberculosis. Int J Tuberc Lung D, 2003, 7(7):652-659.
20. Kim BJ, Lee KH, Yun YJ, et al. Simultaneous identification of rifampin-resistant Mycobacterium tuberculosis and nontuberculous mycobacteria by polymerase chain reaction-single strand conformation polymorphism and sequence analysis of the RNA polymerase gene (rpoB). J Microbiol Meth, 2004, 58(1):111-118.
21. Wu X, Zhang J, Zhuang Y, et al. Molecular mechanisms of drug resistance in mycobacterium tuberculosis clinical isolates. CMJ, 1999, 112(6):524-528.
22. Devillé WL, Buntinx F, Bouter LM, et al. Conducting systematic reviews of diagnostic studies:didactic guidelines. BMC Medical Research Methodology, 2002, 2(1):1-13.
23. 柳清云, 孫剛, 高謙.結核分枝桿菌(MTB)異質性耐藥研究進展.復旦學報(醫學版), 2013, 40(1):1-4.
24. Bossuyt PMM, Reitsma JB, Bruns DE, et al. The STARD statement for reporting studies of diagnostic accuracy:Explanation and elaboration. Clin Chem, 2003, 49(1):7-18.
25. Tackmann R'Schuetz G, HammB, et al. Quality of the reporting of diagnostic accuracy studies:STARD (Standards for the Reporting of Diagnostic accuracy studies). Rofo, 2010, 182(8):655-659.

Chinese Journal of Evidence-Based Medicine

PCR-Single-Strand Conformational Polymorphism Method for Detecting rpoB Gene of Rifampin-Resistant Mycobacterium Tuberculosis: A Meta-Analysis

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content