Effect of electroacupuncture on apoptosis of hippocampal neurons in C57BL/6J mice with status epilepticus_Journal of Epilepsy

Authors：

LV Minghui , SU Shaohua , WU Xin , ZHENG Wanjun , ZHANG Songjiang , SUN Ningning ,  GAO Jianfeng

School of Basic Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China;

Corresponding?author：

GAO Jianfeng, Email: jc.gjfeng@hotmail.com

Keywords：

Electroacupuncture; Status epilepticus; Hippocampus; Neurons; Apoptosis; Mice

DOI：

10.7507/2096-0247.20180036

Video：

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ObjectiveTo investigate the effect of electroacupuncture on the apoptosis of hippocampal neurons in C57BL/6J mice with status epilepticus by observing the changes of hippocampal subtle neuron pathology and apoptosis.MethodsMale C57BL/6J mice were used to prepare epileptic status models of lithium-pilocarpine mice, and then 7-day electroacupuncture stimulation (Baihui, Fengfu) were given to the mice model. Open field experiment and new object recognition experiment were performed to observe the changes of cognitive abilities. The pathological changes of hippocampal neurons were detected by HE staining. Hippocampal apoptosis protein (Caspase-3) and microtubule-associated protein (MAP-2) were detected by immunohistochemistry. Effect of electroacupuncture on apoptosis of hippocampal neurons in C57BL/6J mice with status epilepticus were recorded.Results① Compared with the control group, the vertical movement, modification times, and number of crossings of the model group all decreased significantly (P<0.000 1,P<0.000 1,P<0.000 1), and their cognitive ability decreased significantly (P<0.01). Compared with the model group, vertical movements, modification times, and number of crossings were increased in the electroacupuncture (EA) group (P<0.01,P<0.05,P<0.05), and the cognitive ability of new objects was increased (P<0.01). ② HE staining showed that the model group had significant damage to the hippocampal neurons of mice, and the cells swelled, nuclear collapsed and vacuoles appeared. In the EA group, the injury of hippocampal neurons was alleviated, and cell edema and vacuolization were alleviated. ③ Immunohistochemistry showed that compared with the control group, the IOD of the Caspase-3 positive cells in the hippocampus of the model group increased significantly (P<0.000 1), and the IOD of the MAP-2 positive cells decreased significantly (P<0.01); Compared with the electroacupuncture, the IOD of the Caspase-3 positive cells in the hippocampus of the mice decreased (P<0.05), and the IOD of the MAP-2 positive cells increased (P<0.05).ConclusionsElectroacupuncture can improve the pathological changes of hippocampal neurons in C57BL/6J mice with status epilepticus, promote cytoskeletal repair, reduce neuronal apoptosis in hippocampus, and antagonize the damage of hippocampal neurons induced by status epilepticus.

Citation： LV Minghui, SU Shaohua, WU Xin, ZHENG Wanjun, ZHANG Songjiang, SUN Ningning, GAO Jianfeng. Effect of electroacupuncture on apoptosis of hippocampal neurons in C57BL/6J mice with status epilepticus. Journal of Epilepsy, 2018, 4(3): 207-213. doi: 10.7507/2096-0247.20180036 Copy

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12.	劉友平, 劉田, 畢航, 等. 品系、性別和生物節律對小鼠曠場實驗的影響. 西安交通大學學報(醫學版), 2014, 35(5): 634-638.
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19.	昂文平, 楊帆, 沈德凱, 等. 針刺對戊四唑致癇大鼠海馬神經元超微結構的影響. 中國中醫急癥, 2012, 21(03): 380-382.
20.	Zeng XC, Chen SY, Gao Q, et al. The expression of G protein-coupled receptor kinase 5 and its interaction with dendritic marker microtubule-associated protein-2 after status epilepticus. Epilepsy Res, 2017, 138: 62-70.

1. 呂夢娜, 張洪. 難治性癲癇持續狀態研究進展.中華實用診斷與治療雜志, 2015, 3(29): 212-214.
2. Elger CE, Schmidt D. Modern management of epilepsy: a practical approach. Epilepsy Behav, 2008, 12(4): 501-539.
3. Kobow K, Auvin S, Jensen F, et al. Finding a better drug for epilepsy: antiepileptogenesis targets. Epilepsia, 2012,53(11): 1868-1876.
4. 張冬青. 抗癲癇藥物對癲癇幼鼠認知的影響及其機制的研究. 山東大學, 2013: 21-26/56-60.
5. 高劍峰. 海馬神經干細胞增生分化及影響因素. 鄭州大學, 博士學位論文, 2010: 36-40.
6. Racine RJ. Modification of seizure activity by electrical Ⅱ motor seizure. Electroencephalogr Clin Neurophysiol, 1972, 32(3): 281-294.
7. 余曙光. 實驗針灸學. 上海, 上海科學技術出版社, 2009: 149-150.
8. 陳少宗. 現代針灸學理論與臨床應用. 濟南, 黃河出版社, 1990: 36-37.
9. 武鑫, 鄭婉君, 李琳, 等. 不同療程電針對 X 線照射小鼠行為學影響實驗研究. 中華放射腫瘤學雜志, 2016, 25(6): 634-637.
10. Fan XW, Chen F, Chen Y, et al. Electroacupuncture prevents cognitive impairments by regulating the early changes after brain irradiation in rats. PLoS One, 2015, 10(4): e0122087.
11. 胡明, 朱坤, 張建水, 等. 小鼠顳葉癲癇慢性期 CA3 區損傷對齒狀回神經再生的影響. 西安交通大學學報(醫學版), 2016, 4(37): 473-477.
12. 劉友平, 劉田, 畢航, 等. 品系、性別和生物節律對小鼠曠場實驗的影響. 西安交通大學學報(醫學版), 2014, 35(5): 634-638.
13. Alnemri ES, Livingston DJ, Nicholson DW, et al. Human ICE/CED-3 protease nomenclature. Cell, 1996, 87(2): 171.
14. Salvesen GS. Caspases:opening the boxes and interpreting the arrows. Cell Death Differ, 2002, 9(1): 3-5.
15. Ghavami S, Hashemi M, Ande SR, et al. Apoptosis and cancer: mutations within caspase genes. J Med Genet, 2009, 46(8): 497-510.
16. Cohen GM. Caspases: the executioners of apoptosis. Biochem J, 1997, 326(Pt1): 1-16.
17. Lim RW, Halpain S. Regulated association of microtubule-associated protein 2 (MAP2) with Src and Grb2: evidence for MAP2 as a scaffolding protein. J Biol Chem, 2000, 275(27): 20578-20587.
18. Rosser AE, Tyers P, ter Borg M, et al. Co-expression of MAP-2 and GFAP in cells developing from rat EGF responsive precursor cells. Brain Res Dev Brain Res, 1997, 98(2): 291-295.
19. 昂文平, 楊帆, 沈德凱, 等. 針刺對戊四唑致癇大鼠海馬神經元超微結構的影響. 中國中醫急癥, 2012, 21(03): 380-382.
20. Zeng XC, Chen SY, Gao Q, et al. The expression of G protein-coupled receptor kinase 5 and its interaction with dendritic marker microtubule-associated protein-2 after status epilepticus. Epilepsy Res, 2017, 138: 62-70.

Journal of Epilepsy

Effect of electroacupuncture on apoptosis of hippocampal neurons in C57BL/6J mice with status epilepticus

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