目的:探討Livin基因在人膽管癌組織及膽管癌細胞系中的表達情況及其與膽管癌發
生發展之間的關系。方法:采用免疫組織化學技術（SP法）檢測Livin基因蛋白在45例人膽管
癌標本及及40例癌旁膽管組織、20例正常膽管組織標本中的表達；同時采用RTPCR法及SP
法檢測了Livin基因mRNA和蛋白在人膽管癌細胞系QBC939及非腫瘤細胞系HT1080中表達。
結果:Livin在膽管癌組織中表達陽性率為57.8%，而癌旁膽管組織、非癌膽管組織中未能檢
測到Livin表達。Livin表達與性別、年齡、腫瘤大小及腫瘤分化程度無關。在有淋巴結轉組
中，Livin陽性表達率（70.4%）明顯高于無淋巴結轉移組（38.9%）。在人膽管癌細胞QBC939
中，Livin mRNA及蛋白均特異性表達，而非腫瘤細胞系HT1080未見Livin表達。結論:Livin
基因在人類膽管癌組織和細胞系中選擇性高表達，其可能與膽管癌發生、發展及預后密切相
關。
Citation：
TANG Zhihua,CHEN Jisheng,YE Lin,YU Jiexiong. Expression and Significance of Livin in Human Cholangiocarcinoma Tissues and Cell Line. West China Medical Journal, 2009, 24(1): 67-69. doi:
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Copyright ? the editorial department of West China Medical Journal of West China Medical Publisher. All rights reserved
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Vucic D，Deshayes K，Ackerly H，et al .SMAC negatively regulates the antiapoptotic activity of melanoma inhibitor of apoptosis （MLIAP）[J].J Biol Chem，2002，277 （14）:12275-12279..
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Kim D K，Alvarado C S，Abramowsky C R，et al.Expression of inhibitorofapoptosis protein （IAP） Livin by neuroblastoma cells:correlation with prognostic factors and outcome[J].Pediatr Dev Pathol，2005，8（6）:621-629.
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- 1. Kasof GM，Gomes BC.Livin，a novel inhibitor of apoptosis protein family member[J].J Biol Chem，2001，276（5）:3238-3246..
- 2. Gazzaniga P，Gradilone A，Giuliani L，et al.Expression and prognostic significance of livin，survivin and other apoptosis related genes in the progression of superficial bladder cancer[J].Ann Oncol，2003，14（1）:85-90..
- 3. Yagihashi A，Ohmura T，Asanuma K，et al.Detection of autoantibodies to survivin and livin in sera frompatients with breast cancer[J].Clin Chim Acta，2005，362（12）:125-130..
- 4. Burke EC，Jamagin WR，Hoehwald SN，et al.Hilar cholangiocarcinoma:patterns of spread，the importance of hepatic resection for curative operation，and a presurgical clinical staging system[J].Ann Surg，1998，228:385一394..
- 5. Isa T，Tomita S，Nakaehi A，et al.Analysis of microsatellite instability，Kras gene mutation and P53 protein over expression intrahepatic cholangiocarcinoma[J]. Hepatogastroenterology，2002，49:604-608..
- 6. Vucic D，Deshayes K，Ackerly H，et al .SMAC negatively regulates the antiapoptotic activity of melanoma inhibitor of apoptosis （MLIAP）[J].J Biol Chem，2002，277 （14）:12275-12279..
- 7. Kim D K，Alvarado C S，Abramowsky C R，et al.Expression of inhibitorofapoptosis protein （IAP） Livin by neuroblastoma cells:correlation with prognostic factors and outcome[J].Pediatr Dev Pathol，2005，8（6）:621-629.
