Effects of IL-6 on Apoptosis Induced by Hydrogen Peroxide in Alveolar Epithelial Cells_West China Medical Journal

Authors：

1Department of Respiratory Medicine;2Department of Laboratory Medicine,West China Hospital,Sichuan University,Chengdu 610041,China;

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Keywords：

IL-6; 凋亡; 過氧化氫; caspase-3; caspase-9

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研究白介素6(IL-6)在過氧化氫誘導的肺泡上皮細胞凋亡中的作用及其機制。方法：以人Ⅱ型肺泡上皮細胞A549細胞株為實驗對象，MTT法檢測IL-6對A549細胞增殖活性的影響，TUNEL檢測法檢測IL-6對A549細胞凋亡率的影響，Western blot觀察caspase-3和caspase-9蛋白量的變化。結果：IL-6能夠增加A549細胞的增殖活性，減少其凋亡。與模型組相比，IL-6干預組的caspase-3和caspase-9的蛋白表達量明顯降低,有統計學意義。結論：IL-6能夠抑制過氧化氫誘導的肺泡上皮細胞凋亡，并且與caspase-3和caspase-9蛋白的表達量下降有關。提示IL-6對肺泡上皮細胞的保護作用可能是通過抑制細胞凋亡相關蛋白來實現的。

Citation： YANG Dongmei,DING Yu,YING Binwu,et al.. Effects of IL-6 on Apoptosis Induced by Hydrogen Peroxide in Alveolar Epithelial Cells. West China Medical Journal, 2008, 23(1): 98-100. doi: Copy

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1. Simpson RJ,Hammacher A,Smith DK,et al.Interleukin-6:structure-function relationships[Ｊ].Protein Sci，1997,6（５）:925-955..
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4. Jin XL,Zimmers TA,Perez EA,et al.Paradoxical effects of short-and long-term interleukin-6 exposure on liver injury and repair[J].Hepatology,2006,43(3):474-484..
5. Yamashita T,Sawamoto S，Suzuki S,et al.Blockade of interleukin-6 signaling aggravates ischemic cerebral damage in mice:possible involvement of Stat3 activation in the protection of neurons[J].J Neurochem，2005,94(2):459-468.
6. Hengartner MO.The biochemistry of apoptosis[Ｊ].Nature,2000,407:770-776..

West China Medical Journal

Effects of IL-6 on Apoptosis Induced by Hydrogen Peroxide in Alveolar Epithelial Cells

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