β-連環素基因在乳腺增生及癌組織中表達和突變的研究_《中國普外基礎與臨床雜志》

作者：

劉臻 ¹ , 崔東旭 ¹ , 劉寶林 ¹ , 馬文鋒 ² , 張強 ²

1.中國醫科大學盛京醫院普外一科(沈陽110004);;
2.中國醫科大學附屬第一醫院普外三科（沈陽110001）;

關鍵詞：

乳腺腫瘤 β-連環素基因聚合酶鏈反應免疫組織化學

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目的　檢測β-連環素基因在乳腺增生及癌組織中的表達和突變情況，探討其在乳腺癌發生中的作用。
方法　應用免疫組織化學染色法和PCR-SSCP檢測42例乳腺癌組織、15例乳腺單純性增生組織及15例乳腺非典型增生組織中β-連環素基因的表達及突變情況。
結果　乳腺單純性增生組織中β-連環素表達正常，異常表達率為0。乳腺非典型增生組織和乳腺癌組織中β-連環素表達出現異質性，異常表達率分別為26.7%(4/15)和59.5%(25/42)，明顯高于單純性增生組織（P＜0.05），且前兩者間差異亦有統計學意義（P＜0.05）。乳腺單純性增生、非典型增生和癌組織中均未發現β-連環素基因外顯子3的突變。
結論　β-連環素異常表達在人乳腺癌發生過程中起到重要作用，但β-連環素基因突變并不是該蛋白異常表達的原因，需要進一步研究該蛋白異常表達的機理。

引用本文： 劉臻,崔東旭,劉寶林,馬文鋒,張強. β-連環素基因在乳腺增生及癌組織中表達和突變的研究. 中國普外基礎與臨床雜志, 2007, 14(6): 685-688. doi: 復制

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9.	Fearnhead NS, Britton MP, Bodmer WF. The ABC of APC [J]. Hum Mol Genet, 2001; 10(7)∶721.
10.	Bankfalvi A, Terpe HJ, Breukelmann D, et al. Immunophenotypic and prognostic analysis of E-cadherin and beta-catenin expression during breast carcinogenesis and tumour progression: a comparative study with CD44 [J]. Histopathology, 1999; 34(1)∶25.
11.	Michaelson JS, Leder P. beta-catenin is a downstream effector of Wnt-mediated tumorigenesis in the mammary gland [J]. Oncogene, 2001; 20(37)∶5093.
12.	Imbert A, Eelkema R, Jordan S, et al. Delta N89 beta-catenin induces precocious development, differentiation, and neoplasia in mammary gland [J]. J Cell Biol, 2001; 153(3)∶555.
13.	Miyaki M, Iijima T, Kimura J, et al. Frequent mutation of beta-catenin and APC genes in primary colorectal tumors from patients with hereditary nonpolyposis colorectal cancer [J]. Cancer Res, 1999; 59(18)∶4506.
14.	Miyoshi Y, Iwao K, Nagasawa Y, et al. Activation of the beta-catenin gene in primary hepatocellular carcinomas by somatic alterations involving exon 3 [J]. Cancer Res, 1998; 58(12)∶2524.
15.	Fukuchi T, Sakamoto M, Tsuda H, et al. Beta-catenin mutation in carcinoma of the uterine endometrium [J]. Cancer Res, 1998; 58(16)∶3526.
16.	Palacios, Gamallo C. Mutations in the β-catenin gene (CTNNB1) in endometrioid ovarian carcinomas [J]. Cancer Res, 1998; 58(7)∶1344.
17.	Voeller HJ, Truica CI, Gelmann EP. Beta-catenin mutations in prostate cancer [J]. Cancer Res, 1998; 58(12)∶2520.
18.	Zhuang SM, Wiseman RW, Soderkvist P. et al. Frequent mutations of the Trp53, Hras1 and beta-catenin (Catnb) genes in 1, 3-butadiene-induced mammary adenocarcinomas in B6C3F1 mice [J]. Oncogene, 2002; 21(36)∶5643.
19.	Jonsson M, Borg A, Nilbert M, et al. Involvement of adenomatous polyposis coli (APC)/beta-catenin signalling in human breast cancer [J]. Eur J Cancer, 2000; 36(2)∶242.
20.	Suriano G, Vrcelj N, Senz J, et al. beta-catenin (CTNNB1) gene amplification: a new mechanism of protein overexpression in cancer [J]. Genes Chromosomes Cancer, 2005; 42(3)∶238.
21.	Mao J, Wang J, Liu B, et al. Low-density lipoprotein receptor-related protein-5 binds to Axin and regulates the canonical Wnt signaling pathway [J]. Mol Cell, 2001; 7(4)∶801.

1. 　劉臻，張強，馬文鋒, 等. 上皮鈣黏蛋白和α-連接素的表達與乳腺癌生物學特性的關系 [J]. 中國普外基礎與臨床雜志, 2005; 12（3）∶226.
2. 　劉臻, 張強, 馬文鋒, 等. 上皮鈣黏蛋白、 α-連環素和β-連環素在乳腺癌中的表達及意義 [J]. 中華外科雜志, 2005; 43(5)∶307.
3. 　Chang HJ, Jee CD, Kim WH. Mutation and altered expression of beta-catenin during gallbladder carcinogenesis [J]. Am J Surg Pathol, 2002; 26(6)∶758.
4. 　Rashid A, Gao YT, Bhakta S, et al. Beta-catenin mutations in biliary tract cancers: a population-based study in China [J]. Cancer Res, 2001; 61(8)∶3406.
5. 　Park WS, Oh RR, Park JY, et al. Frequent somatic mutations of the beta-catenin gene in intestinal-type gastric cancer [J]. Cancer Res, 1999; 59(17)∶4257.
6. 　董涇青, 沈祖楹, 于揚, 等. β-catenin和APC蛋白在甲狀腺腫瘤中的表達及意義 [J]. 貴陽醫學院學報, 2002; 27(6)∶471.
7. 　Muller T, Choidas A, Reichmann E, et al. Phosphorylation and free pool of beta-catenin are regulated by tyrosine kinases and tyrosine phosphatases during epithelial cell migration [J]. J Biol Chem, 1999; 274(15)∶10173.
8. 　Karim R, Tse G, Putti T, et al. The significance of the Wnt pathway in the pathology of human cancers [J]. Pathology, 2004; 36(2)∶120.
9. 　Fearnhead NS, Britton MP, Bodmer WF. The ABC of APC [J]. Hum Mol Genet, 2001; 10(7)∶721.
10. 　Bankfalvi A, Terpe HJ, Breukelmann D, et al. Immunophenotypic and prognostic analysis of E-cadherin and beta-catenin expression during breast carcinogenesis and tumour progression: a comparative study with CD44 [J]. Histopathology, 1999; 34(1)∶25.
11. 　Michaelson JS, Leder P. beta-catenin is a downstream effector of Wnt-mediated tumorigenesis in the mammary gland [J]. Oncogene, 2001; 20(37)∶5093.
12. 　Imbert A, Eelkema R, Jordan S, et al. Delta N89 beta-catenin induces precocious development, differentiation, and neoplasia in mammary gland [J]. J Cell Biol, 2001; 153(3)∶555.
13. 　Miyaki M, Iijima T, Kimura J, et al. Frequent mutation of beta-catenin and APC genes in primary colorectal tumors from patients with hereditary nonpolyposis colorectal cancer [J]. Cancer Res, 1999; 59(18)∶4506.
14. 　Miyoshi Y, Iwao K, Nagasawa Y, et al. Activation of the beta-catenin gene in primary hepatocellular carcinomas by somatic alterations involving exon 3 [J]. Cancer Res, 1998; 58(12)∶2524.
15. 　Fukuchi T, Sakamoto M, Tsuda H, et al. Beta-catenin mutation in carcinoma of the uterine endometrium [J]. Cancer Res, 1998; 58(16)∶3526.
16. 　Palacios, Gamallo C. Mutations in the β-catenin gene (CTNNB1) in endometrioid ovarian carcinomas [J]. Cancer Res, 1998; 58(7)∶1344.
17. 　Voeller HJ, Truica CI, Gelmann EP. Beta-catenin mutations in prostate cancer [J]. Cancer Res, 1998; 58(12)∶2520.
18. 　Zhuang SM, Wiseman RW, Soderkvist P. et al. Frequent mutations of the Trp53, Hras1 and beta-catenin (Catnb) genes in 1, 3-butadiene-induced mammary adenocarcinomas in B6C3F1 mice [J]. Oncogene, 2002; 21(36)∶5643.
19. 　Jonsson M, Borg A, Nilbert M, et al. Involvement of adenomatous polyposis coli (APC)/beta-catenin signalling in human breast cancer [J]. Eur J Cancer, 2000; 36(2)∶242.
20. 　Suriano G, Vrcelj N, Senz J, et al. beta-catenin (CTNNB1) gene amplification: a new mechanism of protein overexpression in cancer [J]. Genes Chromosomes Cancer, 2005; 42(3)∶238.
21. 　Mao J, Wang J, Liu B, et al. Low-density lipoprotein receptor-related protein-5 binds to Axin and regulates the canonical Wnt signaling pathway [J]. Mol Cell, 2001; 7(4)∶801.

《中國普外基礎與臨床雜志》

β-連環素基因在乳腺增生及癌組織中表達和突變的研究

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《中國普外基礎與臨床雜志》

β-連環素基因在乳腺增生及癌組織中表達和突變的研究

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

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