甘氨酸對鼠肝熱缺血再灌注后肝竇內皮細胞損傷的保護作用_《中國普外基礎與臨床雜志》

作者：

舒明 , 熊本京 , 傅華群 , 蔣筱強 , 鄒書兵

江西醫學院第二附屬醫院普外一科（南昌330006）;

關鍵詞：

肝臟缺血再灌注損傷甘氨酸受體

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目的探討甘氨酸對大鼠肝臟熱缺血再灌注后肝竇內皮細胞損傷的保護作用及其機理。方法取健康雄性SD大鼠72只，制備成鼠肝熱缺血再灌注模型,而后隨機分成正常對照組、缺血再灌注組、士的寧＋甘氨酸治療組和甘氨酸治療組, 每組各18只。分別于再灌注后1、3、24 h檢測血漿中內皮素（ET）、透明質酸（HA）、腫瘤壞死因子α （TNFα）、谷丙轉氨酶（ALT）及肝組織中超氧化物歧化酶（SOD）含量的變化，并在光鏡下觀察肝竇內皮細胞的病理改變。結果在再灌注后的不同時點，甘氨酸治療組中ET、HA、TNFα及ALT含量較缺血再灌注組顯著降低(P＜0.01或P＜0.05），SOD值相應升高，同時光鏡下肝竇內皮細胞的病理變化明顯改善，士的寧可部分拮抗甘氨酸的作用。結論甘氨酸對鼠肝熱缺血再灌注后肝竇內皮細胞的損傷具有保護作用。推測這種作用可能與肝竇內皮細胞及枯否細胞膜上的甘氨酸受體密切相關。

引用本文： 舒明,熊本京,傅華群,蔣筱強,鄒書兵. 甘氨酸對鼠肝熱缺血再灌注后肝竇內皮細胞損傷的保護作用. 中國普外基礎與臨床雜志, 2003, 10(4): 351-354. doi: 復制

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1. Hayashi H, Chaudry IH, Clemens MG, et al. Hepatic ischemic modals for determining the effects of ATPMgCl2 treatment [Ｊ]. J Surg Res, 1986; 40(2)∶167.
2. 熊成龍，胡虹，陳孝平，等.常溫下肝缺血再灌注肝血竇內皮細胞損傷 [Ｊ]. 中華外科雜志, 2000; 38(4)∶297.
3. Gujral JS, Bucci TJ, Farhood A, et al. Mechanism of cell death during warm hepatic ischemiareperfusion in rats: apoptosis or necrosis? [Ｊ]. Hepatology, 2001; 33(2)∶397.
4. Hisama N, Yamaguchi Y, Ishiko T, et al. Kupffer cell production of cytokineinduced neutrophil chemoattractant following ischemia/reperfusion injury in rats [Ｊ]. Hepatology, 1996; 24(5)∶1193.
5. Kourembanas S, Marsden PA, McQuillan LP, et al. Hypoxia induces endothelin gene expression and secretion in cultured human endothelium [Ｊ]. J Clin Invest, 1991; 88(3)∶1054.
6. Itasaka H, Kishikawa K, Suehiro T, et al. Serum hyaluronic acid for the assessment of graft viability in porcine liver transplantation [Ｊ]. Surg Today, 1994; 24(8)∶719.
7. Ikejima K, Qu W, Stachlewitz RF, et al. Kupffer cells contain a glycinegated chloride channel [Ｊ].Am J Physiol, 1997; 272(6 Pt 1)∶G1581.
8. Zhang Y, Ikejima K, Honda H, et al.Glycine prevents apoptosis of rat sinusoidal endothelial cells caused by deprivation of vascular endothelial growth factor [Ｊ]. Hepatology, 2000; 32(3)∶542.
9. Nishimura Y, Romer LH, Lemasters, et al. Mitochondrial dysfunction and cytoskeletal disruption during chemical hypoxia to cultured rat hepatic sinusoidal endothelial cells: the pH paradox and cytoprotection by glucose, acidotic pH, and glycine [Ｊ]. Hepatology, 1998; 27(4)∶1039.

《中國普外基礎與臨床雜志》

甘氨酸對鼠肝熱缺血再灌注后肝竇內皮細胞損傷的保護作用

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《中國普外基礎與臨床雜志》

甘氨酸對鼠肝熱缺血再灌注后肝竇內皮細胞損傷的保護作用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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Content

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