;ObjectiveUsing human tumor necrosis factoralpha (TNFα) genetransduced human liver cancer cell BEL7404 as tumor vaccine, to study the effect of immune rejection to mice liver cancer implanted tumors. MethodsMice were divided into five groups, and were inoculated with TNFα genetransduced BEL7404 cells which irradiated with 60Co (BEL7404TNFCo group), TNFα genetransduced BEL7404 cells (BEL7404TNF group), BEL7404 cells (BEL7404 group), BEL7404 cell irradiated with 60Co (BEL7404Co group) respectively. Normal saline was injected in control group. Then mice liver cancer H22 cells were implanted to each group, the growth of mice liver cancer implanted tumors was observed. The apoptosis index of implanted tumors was detected by TUNEL method.ResultsCompared to BEL7404 group,BEL7404Co group and control group, the tumor vaccine which did not transduce with TNFα gene and the control group, the tumorigenesis rate of liver cancer implanted tumors was reduced, the growth of implanted tumors was inhibited and the apoptosis of implanted tumors was increased in BEL7404TNFCo group,P＜0.01.There was no difference between BEL7404TNFCo group and BEL7404TNF group,P gt;0.05. ConclusionHuman tumor necrosis factoralpha genetransduced human liver cancer cell can be used as tumor vaccine, it has quite b effect of immune rejection to mice liver cancer implanted tumors.
Citation：
LU Yunfei,QIN Xingan,ZENG Jian,LIN Jinling,LIAO Qinghua. Effect of Tumor Necrosis Factoralpha Genetransduced Tumor Vaccine in Mice Liver Cancer Implanted Tumors. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(4): 366-369. doi:
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Copyright ? the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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- 2. 陸云飛，林進令，邱慶明，等. 原發性肝癌基因治療研究含人TNF基因重組逆轉錄病毒轉移系統的構建與表達 [Ｊ]. 廣西醫科大學學報， 1997； 14（4）∶1.
- 3. 邱慶明，陸云飛. 轉基因人肝癌細胞株（BEL7404/TNF）的建立及其生物活性的初步研究 [Ｊ]. 廣西醫科大學學報， 1999； 16（4）∶408.
- 4. Wright P, Braun R, Babiuk L, et al. Adenovirusmediated TNFalpha gene transfer induces significant tumor regression in mice [Ｊ]. Cancer Biother Radiopharm, 1999; 14(1)∶49.
- 5. Sato Y, Koshita Y, Hirayama M, et al. Augmented antitumor effects of killer cells induced by tumor necrosis factor genetransdued autologous tumor cells from gastrointestinal cancer patients [Ｊ]. Hum Gene Ther, 1996; 7(15)∶1895.
- 6. Koshita Y, Lu Y, Fujii S, et al. Efficacy of TNFalpha genetransduced tumor cells in treatment of established in vivo tumor [Ｊ]. Int J Cancer, 1995; 63(1)∶130.
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