Objective To investigate the pathological characteristics of hepatic energy metabolism changes following hepatic inflow occlusion and the tolerant limit to ischemia in cholestatic rats.
Methods On the day 7 after rats biliary obstruction, the survival rate, hepatic mitochondrial respiratory function, content of ATP, and the ketone body ratio in arterial blood were investigated following the different duration of hepatic ischemia and reperfusion with portal blood bypass.
Results The survival rate on postoperative day 10 was 100%, 100% and 40% subjected to 30, 60 and 90min of hepatic vascular occlusion. The hepatic energy metabolic function was decreased markedly following ischemia, and was increased markedly on 24 hours following reperfusion subjected to 30, 60min of hepatic vascular occlusion, but it had less increase with 90min of hepatic vascular occlusion.
Conclusion The hepatic energy metabolic function injury is reversible in cholestatic rats, and the rats can tolerate hepatic inflow occlusion within 60min, but the hepatic energy metabolic function injury is irreversible after 90min of hepatic occlusion.
Citation：
ZHANG Leida,DONG Jiahong,HAN Benli,et al.. THE EFFECT OF HEPATIC INFLOW OCCLUSION ON HEPATIC ENERGY METABOLISM IN CHOLESTATIC RATS. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2001, 8(1): 12-14. doi:
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Copyright ? the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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- 1. Yamamoto S, Nitta N, Kamiyama Y, et al. Effects of partial deprivation of portal blood on arterial blood ketone body ratio in rabbits [J]. Eur Surg Res, 1989; 21(1)∶98.
- 2. 陳孝平，吳在德，裘法祖.家兔常溫下阻斷肝門最大耐受時限的實驗研究 [J].中華外科雜志，1984； 22(3)∶153.
- 3. 何效東.大鼠門靜脈轉流下耐受入肝血流阻斷安全時限的實驗研究[D].第三軍醫大學碩士研究生論文，1998∶1～46.
- 4. Johnson D.Isolation of liver or kidney mitochondria [J].Methods in Enzymology, 1967; 10(1)∶94.
- 5. Estabrook RW. Mitochondrial respiratory control and the polarographic measurement of ADP/O ratio [J].Methods In Enzymology, 1967; 10(1)∶41.
- 6. 嚴濤，張平，段恒春等.組織ATP、ADP、AMP水平的熒光素酶系測定 [J].第三軍醫大學學報，1987； 9(3)∶294.
- 7. Williamson DH, Mellanby J. βHydroxybutyrate. In:Edited by HV Bergmeyer. Methods of enzymatic analysis [M]. New York:Academic press, In C. 1974∶18361839.
- 8. Mellanby J, Williamson DH. Acetoacetate. In:Edited by HV Bergmeyer. Methods of enzymatic analysis[M].New York:Academic press,In C. 1974∶1446-1451.
