To observe the pathologic changes of normal tissue in nude mice after peritoneal perfusion with hyperthermia, hyper-osmolar solution and mitomycin C (MMC). Fifty BALB/c nunu mices (7-10 weeks old) bearing HT-2 lines were chosen for the study, and were randomly divided into five groups: isotonic solution (control group), hyperosmolar solution (HOS group), HOS plus MMC group, hyperthermia (HT group) and HOS plus HT plus MMC group. After continuous hyperthermic peritoneal perfusion (42℃/30min) with 7.5% NaCl and 5μg/ml MMC, the liver, spleen, small intestine and kidney were examined by light microscopy. Results: ①In HOS and HOS plus MMC groups, no changes of liver, spleen and kidney were found. ②In HT and HOS plus HT plus MMC groups, slight degeneration of liver, hyperemia of spleen, swelling of kidney tubule cells and small intestine were found. ③In HOS plus HT plus MMC group, partial loss of small intestinal villi were also observed. Conclusioin: After continuous hyperthermic perfusion conbined with hyper-osmolar solutions and mitomycin C, a slight injury was showed in normal tissue of nude mice.
Citation：
Cao Hong,Wang Daike,Wu Xiaohua.. EFFECT OF PERITONEAL PERFUSION WITH HYPERTHERMIA, HYPER-OSMOLAR SOLUTIONS AND ANTINEOPLASTIC DRUG ON NORMAL TISSUE IN NUDE MICE. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1999, 6(2): 79-80. doi:
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Copyright ? the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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- 1. 曹紅， 王代科. 溫熱高滲液化療藥物對人大腸癌移植瘤的體內作用. 中華實驗外科雜志， 1996； 13(5)∶121.
- 2. 曹紅， 王代科， 吳小華等. 高滲熱化療對人結腸癌裸鼠移植瘤的病理影響. 第三軍醫大學學報， 1998； 20(2)∶69.
- 3. 史歷， 劉旭， 劉曼杰等. 人結腸腺癌裸鼠皮下和腹腔移植瘤株的建立及主要生物學特性的觀察. 實用腫瘤學雜志， 1993； 16(3)∶34.
- 4. Larkin JM, Edwards WS, Daniel E, et al. Systemic thermotherapy: description of a method and physiologic tolerance in clinical subjects. Cancer, 1977; 40(6)∶3155.
- 5. Zavagno G, Vespa D, Moschin N, et al. Effect of hyperthermia on isolated perfused rat liver. Eur Surg Res, 1989; 21(5)∶243.
- 6. Gilly FN, Carry PY, Sayag AC, et al. Tolerance of intraperitoneal chemohyperthermia with mitomycin C： in vivo study in dogs. Hyperthermia, 1992; 8(5)∶659.
- 7. Tsubouchi S, Kano E, Nishimoto Y, et al. The effect of microwave induced whole body hyperthermia on the mucosa of mouse small intestine. J Radiat Res, 1984; 25(2)∶131.
