Human SW480 colonic cancer cell line was evaluated for its growth response to Octa peptide somatostatin (SMS 201·995, SMS) in vitro by MTT assay and flow cytometry. The results showed that SMS possessed an inhibitive effect on SW480 cell at dose 1.563－200ng/ml, the maximal effective dose was 50ng/ml. Inhibitive effect of SMS did not steadily increase at a dose ＞50ng/ml. It suggests that effect of SMS is achieved via somatotatin receptor. SMS obviously inhibited the synthesis of DNA and protein, and prohibited the SW480 cell shifting from phase G0/G1 in phase S, G2M, which suggests that somatostatin (SS) possessed an inhibitive effect on large intestinal at cancer cell, it is achieved at receptor by inhibiting the synthesis of DNA and protein and prohibiting cell cycle of cancer.
Citation：
Xie Shangkui,He Shuangwu,Cheng Mingzhu,et al.. EFFECTS OF SOMATOSTATIN ON HUMAN SW480 COLONIC CANCER CELL LINE IN VITRO. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1998, 5(3): 138-139. doi:
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- 4. 宋平根，李素文.流式細胞術的原理和應用.第1版.北京：北京師范大學出版社，1992∶254～268.
- 5. DyDy, White RH, Morris DL. SMS 201·995 inhibits in vitro and in vivo growth of human colon cancer. Cancer Res, 1992; 52(7)∶917.
- 6. Miller GV, Farmery SM, Woodhouse LF,et al. Somatostatin binding in normal and malignant human gastrointestinal mucosa. Br J Cancer, 1992; 66(2)∶391.
- 7. Evers BM, Parekh D, Townsend CMJ, et al. Somatostatin and analogues in the treatment of cancer. Am J Surg, 1991; 213(3)∶190.
