Effects of Isoflurane on the Changes of Oxygen Free Radicals and Intercellular Adhesion Molecule-1 Expression in Rats with Liver Ischemia and Reperfusion Injury_West China Medical Journal

Authors：

XU Guangmin ,  LAN Zhixun

Department of Anesthesia, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu, Sichuan 610072, P. R. China;

Corresponding?author：

LAN Zhixun, Email: xgmin2004@163.com

Keywords：

異氟醚; 缺血-再灌注損傷; 肝臟; 細胞間黏附分子-1; 谷胱甘肽

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目的　探討異氟醚通過抑制細胞間黏附分子（ICAM-1）表達參與減輕肝臟缺血-再灌注（IR）損傷的可能調節機制。方法　32只雌性SD大鼠分為4組。A組大鼠行腹腔注射1%戊巴比妥鈉40 mg/kg麻醉，進行手術但不阻斷入肝血流；B組1%戊巴比妥鈉麻醉后行部分肝臟IR；C組大鼠僅接受1.0 MAC異氟醚吸入麻醉，不阻斷血流；D組采用1.0 MAC異氟醚麻醉，建立肝臟IR模型。肝臟缺血60 min，再灌注3 h后取肝組織和血液標本，檢測血清丙氨酸轉氨酶（ALT）和天冬門氨酸轉氨酶（AST）、肝組織ICAM-1和肝組織還原型谷胱甘肽（GSH）、脂質過氧化物丙二醛（MDA）和超氧化物歧化酶（SOD）含量。結果　與戊巴比妥鈉麻醉比較，采用異氟醚處理后明顯降低血清ALT和AST的水平，再灌注肝組織內GSH、SOD含量明顯高于而MDA含量降低，同時抑制肝組織ICAM-1的表達。結論　異氟醚麻醉能夠有效減輕肝臟IR損傷，抑制氧自由基的生成和釋放，具體機制可能與抑制ICAM-1表達致使細胞內GSH含量增加密切相關。

Citation： XU Guangmin,LAN Zhixun. Effects of Isoflurane on the Changes of Oxygen Free Radicals and Intercellular Adhesion Molecule-1 Expression in Rats with Liver Ischemia and Reperfusion Injury. West China Medical Journal, 2012, 27(11): 1660-1663. doi: Copy

1.	徐廣民, 陶國才, 易斌, 等. 異氟醚對大鼠缺血-再灌注損傷肝臟細胞黏附分子-1表達及中性粒細胞浸潤的影響[J]. 臨床麻醉學雜志, 2010, 26(11): 1004-1006.
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5.	Pruitt HM, Langston W, Kevil CG, et al. ICAM-1 cross-linking stimulates endothelial glutathione synthesis[J]. Antioxid Redox Signal, 2007, 9(1): 159-164.
6.	Li L, Yan L, Wang Z, et al, Change of apoptotic status in the human colorectal adenoma-carcinoma sequences and its correlation with carcinogenesis and prognosis[J]. Chin Med J (Engl), 2000, 113(10): 886-888.
7.	Tao KM, Yang LQ, Liu YT, et al. Volatile anesthetics might be more beneficial than propofol for postoperative liver function in cirrhotic patients receiving hepatectomy[J]. Med Hypotheses, 2010, 75(6): 555-557.
8.	Lv X, Wang ZM, Huang SD, et al. Emulsified isoflurane preconditioning reduces lung injury induced by hepatic ischemia/reperfusion in rats[J]. Int J Med Sci, 2011, 8(5): 353-361.
9.	Ko JS, Kim G, Shin YH, et al. The effects of desflurane and isoflurane on hepatic and renal functions after right hepatectomy in living donors[J]. Transplant Proc, 2012, 44(2): 442-444.
10.	Kim M, Park SW, Kim M, et al. Isoflurane activates intestinal sphingosine kinase to protect against renal ischemia-reperfusion-induced liver and intestine injury[J]. Anesthesiology, 2011, 114(2): 363-373.
11.	王雨, 湯禮軍, 戴睿武, 等. p38MAPK信號轉導途徑對早期缺血再灌注期間離體肝臟ICAM1 mRNA表達的影響[J]. 華西醫學, 2009, 24(8): 2087-2091.
12.	葛廷, 王小琦, 廖世奇, 等. 肝缺血再灌注損傷機制及與相關細胞因子關系的研究進展[J]. 甘肅醫藥, 2010, 29(5): 499-503.
13.	Xiong Y, Uys JD, Tew KD, et al. S-glutathionylation: from molecular mechanisms to health outcomes[J]. Antioxid Redox Signal, 2011, 15(1): 233-270.

1. 　徐廣民, 陶國才, 易斌, 等. 異氟醚對大鼠缺血-再灌注損傷肝臟細胞黏附分子-1表達及中性粒細胞浸潤的影響[J]. 臨床麻醉學雜志, 2010, 26(11): 1004-1006.
2. 　黃施偉, 李士通, 王瑩恬, 等. 異氟醚預處理對大鼠肝血竇內皮缺血再灌注損傷的影響[J]. 中華麻醉學雜志, 2007, 27(11): 347-351.
3. 　王旭東, 盧雅立, 王飛, 等. 七氟烷和異氟醚對肝臟缺血再灌注損傷的影響[J]. 中山大學學報(醫學科學版), 2008, 29(1): 28-33.
4. 　Pattillo CB, Pardue S, Shen X, et al. ICAM-1 cytoplasmic tail regulates endothelial glutathione synthesis through a NOX4/PI3-kinase-dependent pathway[J]. Free Radic Biol Med, 2010, 49(6): 1119-1128.
5. 　Pruitt HM, Langston W, Kevil CG, et al. ICAM-1 cross-linking stimulates endothelial glutathione synthesis[J]. Antioxid Redox Signal, 2007, 9(1): 159-164.
6. 　Li L, Yan L, Wang Z, et al, Change of apoptotic status in the human colorectal adenoma-carcinoma sequences and its correlation with carcinogenesis and prognosis[J]. Chin Med J (Engl), 2000, 113(10): 886-888.
7. 　Tao KM, Yang LQ, Liu YT, et al. Volatile anesthetics might be more beneficial than propofol for postoperative liver function in cirrhotic patients receiving hepatectomy[J]. Med Hypotheses, 2010, 75(6): 555-557.
8. 　Lv X, Wang ZM, Huang SD, et al. Emulsified isoflurane preconditioning reduces lung injury induced by hepatic ischemia/reperfusion in rats[J]. Int J Med Sci, 2011, 8(5): 353-361.
9. 　Ko JS, Kim G, Shin YH, et al. The effects of desflurane and isoflurane on hepatic and renal functions after right hepatectomy in living donors[J]. Transplant Proc, 2012, 44(2): 442-444.
10. 　Kim M, Park SW, Kim M, et al. Isoflurane activates intestinal sphingosine kinase to protect against renal ischemia-reperfusion-induced liver and intestine injury[J]. Anesthesiology, 2011, 114(2): 363-373.
11. 　王雨, 湯禮軍, 戴睿武, 等. p38MAPK信號轉導途徑對早期缺血再灌注期間離體肝臟ICAM1 mRNA表達的影響[J]. 華西醫學, 2009, 24(8): 2087-2091.
12. 　葛廷, 王小琦, 廖世奇, 等. 肝缺血再灌注損傷機制及與相關細胞因子關系的研究進展[J]. 甘肅醫藥, 2010, 29(5): 499-503.
13. 　Xiong Y, Uys JD, Tew KD, et al. S-glutathionylation: from molecular mechanisms to health outcomes[J]. Antioxid Redox Signal, 2011, 15(1): 233-270.

West China Medical Journal

Effects of Isoflurane on the Changes of Oxygen Free Radicals and Intercellular Adhesion Molecule-1 Expression in Rats with Liver Ischemia and Reperfusion Injury

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