Research on the Relationship between Urine Components and the Diabetes Early Renal Damage_West China Medical Journal

Authors：

XU Shiru ¹ , CHEN Rui ¹ , WU Chaoyang ¹ , MA Yuhua ² ,  FENG Shun ²

1. Xinjiang Uygur Autonomous Region People’s Hospital, Urumqi, Xinjiang 830001, P. R. China; 2. College of Chemistry and Chemical Engineering, Xinjiang University, Urumqi, Xinjiang, 830046, P. R. China;

Corresponding?author：

FENG Shun, Email: 438231016@qq.com

Keywords：

糖尿病腎病; 尿液; 非蛋白氮; 尿蛋白; 歸一化

Video：

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目的　對尿液特征組分與糖尿病早期腎損害的關系進行了初步探索。方法　對2011年12月－2012年5月間28例2型糖尿病組、33例2型糖尿病腎病組及26例健康對照組尿液中尿蛋白含量和幾種常見非蛋白氮物質，包括肌酸、尿囊素、肌酐、尿酸和假尿嘧啶核苷的濃度進行測定，采用多種歸一化方法對數據進行對比分析，并通過t檢驗減少高效液相色譜測定的變量信息，保留P＜0.05的檢出峰進行主成分分析，獲得分類結果。結果　采用體積歸一化方法，發現健康對照組尿液中肌酸、尿囊素和尿酸的含量與2型糖尿病組和糖尿病腎病組相比，差異均有統計學意義（P＜0.05），2型糖尿病組尿液中尿蛋白的濃度與糖尿病腎病組相比，差異有統計學意義（P＜0.05）。結論　通過肌酸、尿囊素、尿酸和尿蛋白的聯合測定為腎臟損傷程度的監測及療效觀察提供依據，為2型糖尿病患者腎功能損壞的早期預防與診斷進行初步判斷提供了新的方法。

Citation： XU Shiru,CHEN Rui,WU Chaoyang,MA Yuhua,FENG Shun. Research on the Relationship between Urine Components and the Diabetes Early Renal Damage. West China Medical Journal, 2012, 27(10): 1505-1509. doi: Copy

1.	Moreira ED Jr, Neves RC, Nunes ZO, et al. Glycemic control and its correlates in patients with diabetes in Venezuela： results from a nationwide survey[J]. Diabetes Res Clin Pract, 2010, 87(3): 407-414.
2.	梁紅峰, 姚錦繡，譚國據，等. 血清光抑素C測定在糖尿病及高血壓腎病早期診斷的臨床意義[J]. 華西醫學, 2012, 27(6): 845-847.
3.	許茜芳, 姬秋和. 糖尿病腎病早期診斷相關指標的研究進展[J]. 陜西醫學雜志, 2010, 39(7): 880-882.
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7.	彭長華，王昌富，李承彬，等. HPLC同時檢測血清和尿樣中肌酐、假尿苷、尿酸[J]. 色譜, 1998, 16(2): 146-148.
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10.	Liotta E, Gottardo R, Bonizzato L, et al . Rapid and direct determination of creatinine in urine using capillary zone electrophoresis[J]. Clin Chim Acta, 2009, 409(1-2): 52-55.
11.	Zuo Y, Yang Y, Zhu Z, et al . Determination of uric acid and creatinine in human urine using hydrophilic interaction chromatography[J]. Talanta, 2011, 83(5): 1707-1710.
12.	Shingfield KJ, Offer NW. Simultaneous determination of purine metabolites, creatinine and pseudouridine in ruminant urine by reversed-phase highperformance liquid chromatography[J]. J Chromatogr B Biomed Sci Appl, 1999, 723(1-2): 81-94.
13.	Rasmuson T, Björk GR. Urinary excretion of pseudouridine and prognosis of patients with malignant lymphoma[J]. Acta Oncol, 1995, 34(1): 61-67.
14.	Zuo YG, Wang C, Zhou J, et al . Simultaneous determination of creatinine and uric acid in human urine by high-performance liquid chromatography[J]. Anal Sci, 2008, 24(12): 1589-1592.
15.	Alfazema LN, Howells S, Perrett D. Determination of allantoin in biofluids using micellar electrokinetic capillary chromatography[J]. J Chromatogr A, 1998, 817(1-2): 345-352.
16.	Erasmus RT, Okesina B. Microalbuminuria in a clinic population of type 2 Melanesian diabetics: relationship to glycaemic control[J]. Cent Afr J Med, 1999, 45 (11): 291-294.
17.	姚勤，張峻，何瑾，等. 高效液相色譜法測定人血漿中同型半胱氨酸濃度[J]. 華西醫學, 2012, 27(6): 809-811.

1. 　Moreira ED Jr, Neves RC, Nunes ZO, et al. Glycemic control and its correlates in patients with diabetes in Venezuela： results from a nationwide survey[J]. Diabetes Res Clin Pract, 2010, 87(3): 407-414.
2. 　梁紅峰, 姚錦繡，譚國據，等. 血清光抑素C測定在糖尿病及高血壓腎病早期診斷的臨床意義[J]. 華西醫學, 2012, 27(6): 845-847.
3. 　許茜芳, 姬秋和. 糖尿病腎病早期診斷相關指標的研究進展[J]. 陜西醫學雜志, 2010, 39(7): 880-882.
4. 　Caubet C, Lacroix C, Decramer S, et al . Advances in urinary proteome analysis and biomarker discovery in pediatric renal disease[J]. Pediatr Nephrol, 2010, 25(1): 27-35.
5. 　Lowry OH, Rosebrough NJ, Farr AL, et al . Protein measurement with the Folin phenol reagent[J]. J Biol Chem, 1951, 193(1): 265-275.
6. 　Carlsson N, Borde A, Wolfel S, et al . Quantification of protein concentration by the Bradford method in the presence of pharmaceutical polymers[J]. Anal Biochem, 2011, 411(1): 116-121.
7. 　彭長華，王昌富，李承彬，等. HPLC同時檢測血清和尿樣中肌酐、假尿苷、尿酸[J]. 色譜, 1998, 16(2): 146-148.
8. 　Grabowska I, Chudy M, Dybko A, et al . Determination of creatinine in clinical samples based on flow-through microsystem[J]. Anal Chim Acta, 2005, 540(1): 181-185.
9. 　Husková R, Chrastina P, Adam T, et al . Determination of creatinine in urine by tandem mass spectrometry[J]. Clin Chim Acta, 2004, 350(1-2): 99-106.
10. 　Liotta E, Gottardo R, Bonizzato L, et al . Rapid and direct determination of creatinine in urine using capillary zone electrophoresis[J]. Clin Chim Acta, 2009, 409(1-2): 52-55.
11. 　Zuo Y, Yang Y, Zhu Z, et al . Determination of uric acid and creatinine in human urine using hydrophilic interaction chromatography[J]. Talanta, 2011, 83(5): 1707-1710.
12. 　Shingfield KJ, Offer NW. Simultaneous determination of purine metabolites, creatinine and pseudouridine in ruminant urine by reversed-phase highperformance liquid chromatography[J]. J Chromatogr B Biomed Sci Appl, 1999, 723(1-2): 81-94.
13. 　Rasmuson T, Björk GR. Urinary excretion of pseudouridine and prognosis of patients with malignant lymphoma[J]. Acta Oncol, 1995, 34(1): 61-67.
14. 　Zuo YG, Wang C, Zhou J, et al . Simultaneous determination of creatinine and uric acid in human urine by high-performance liquid chromatography[J]. Anal Sci, 2008, 24(12): 1589-1592.
15. 　Alfazema LN, Howells S, Perrett D. Determination of allantoin in biofluids using micellar electrokinetic capillary chromatography[J]. J Chromatogr A, 1998, 817(1-2): 345-352.
16. 　Erasmus RT, Okesina B. Microalbuminuria in a clinic population of type 2 Melanesian diabetics: relationship to glycaemic control[J]. Cent Afr J Med, 1999, 45 (11): 291-294.
17. 　姚勤，張峻，何瑾，等. 高效液相色譜法測定人血漿中同型半胱氨酸濃度[J]. 華西醫學, 2012, 27(6): 809-811.

West China Medical Journal

Research on the Relationship between Urine Components and the Diabetes Early Renal Damage

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