Expression of Cathepsin B in Bladder Transitional Cell Carcinoma Tissues and Its Significance_West China Medical Journal

Authors：

SHI Tao ¹ , GAO Yan ² , GUO Yonglian ¹

1. Department of Urology; 2. Department of VIP; the Affiliated Xiangyang Hospital of Hubei University of Medicine, Xiangyang, Hubei 441000, P. R. China;

Corresponding?author：

Keywords：

組織蛋白酶B; 膀胱移行癌細胞; 表達

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目的　探討組織蛋白酶B（CB）在膀胱移行細胞癌（TCC）中的表達以及其與TCC浸潤的關系。方法　取TCC標本40例，TCC分級Ⅰ級23例，Ⅱ～Ⅲ級17例；表淺型TCC（Tis，Ta，T1期） 25例，浸潤型TCC（T2～4期）15例。另取10例正常膀胱組織作為對照。用鏈霉素抗生物素蛋白-過氧化物酶連接法行CB免疫組織化學染色觀察并計算CB陽性細胞百分率。結果　正常膀胱組織中基質無明顯著色；在TCC癌組織中，CB可為細胞染色，部分基質亦有染色，部分毛細血管內皮細胞及部分成纖維細胞CB表達陽性，在癌周血管內皮細胞的陽性著色CB表達增強。CB在分級和分期高的癌組織中多為彌散陽性染色。TCC分級Ⅰ級組、TCC分級Ⅱ～Ⅲ級組、TCC分期表淺型組、TCC分期浸潤型組及正常對照組的CB陽性細胞百分比分別為10.53% ± 3.76%、21.52% ± 3.58%、11.32% ±2.69%、20.57% ± 3.25%、0.11% ± 0.18%，TCC各組均高于正常對照組（P＜0.01）；TCC分級Ⅱ～Ⅲ級組高于TCC分級Ⅰ級組，TCC分期浸潤型組高于TCC分期表淺型組，差異均有統計學意義（P＜0.01）。結論　CB可能成為判斷TCC進展和預后的重要指標。

Citation： SHI Tao,GAO Yan,GUO Yonglian. Expression of Cathepsin B in Bladder Transitional Cell Carcinoma Tissues and Its Significance. West China Medical Journal, 2012, 27(6): 892-894. doi: Copy

1.	Buck MR, Karustis DG, Day NA, et al. Degradation of extracellular-matrix proteins by human cathepsin B from normal and tumour tissues[J]. Biochem J, 1992, 282(Pt 1): 273-278.
2.	Satoh Y, Higashi T, Nouso K, et al. Cathepsin B in the growth of colorectal cancer: suppressive effect of leupeptin on the growth of DMH-induced rat colon neoplasm[J]. Acta Med Okayama, 1996，50(6): 299-303.
3.	Shuja S, Murnane MJ. Marked increases in cathepsin B and L activities distinguish papillary carcinoma of the thyroid from normal thyroid or thyroid with non-neoplastic disease[J]. Int J Cancer, 1996, 66(4): 420-426.
4.	Werle B, Ebert W, Klein W, et al. Cathepsin B in tumors， normal tissue and isolated cells from the human lung[J]. Anticancer Res, 1994, 14(3A): 1169-1176.
5.	Guenette RS, Mooibroek M, Wong K, et al. Cathepsin B, a cysteine protease implicated in metastatic progression is also expressed during regression of the rat prostate and mammary glands[J]. Eur J Biochem, 1994, 226(2): 311-321.
6.	Sloane BF, Rozhin J, Robinson D, et al. Role for cathepsin B and cystatins in tumor growth and progression[J]. Biol Chem Hoppe Seyler, 1990, 371 Suppl: 193-198.
7.	Sivaparvathi M, McCutcheon I, Sawaya R， et al. Expression of cysteine protease inhibitors in human gliomas and meningiomas[J]. Clin Exp Metastasis, 1996, 14(4): 344-350.
8.	Farinati F, Herszenyi L, plebani M, et al. Increased levels of cathepsin B and L, urokinase-type plasminogen activator and its inhibitor type-1 as an early event in gastric carcinogenesis[J]. Carcinogenesis, 1996, 17(12): 2581-2587.
9.	Sinha AA, Gleason DF, Staley NA, et al. Cathepsin B in angiogenesis of hunan prostate: an immunohistochemical and immunoelectron microscopic analysis[J]. Anat Res, 1995, 241(3): 353-362.
10.	Eiján A, Sandes EO, Riveos MD, et al. High expression of cathepsin B in trasitional bladder carcinoma correlates with tumor invasion[J]. Cancer, 2003, 98(2): 262-268.
11.	Visscher DW, Sloane BF，Sameni M，et al.Clincopathologic significance of cathepsin B immunostaining in transitional neoplasia[J]. Mod Pathol，1994，7(1): 76-81.
12.	Staack A， Koenig F, Daniltichenko D, et al. Cathepsin B, H and activities in urine of patients with transitional cell carcinoma of the bladder[J]. Urology, 2002, 59(2): 308-312.
13.	Eiján AM, Sandes E, Puricelli L, et al. Cathepsin B levels in urine from bladder cancer patients[J]. Oncol Res, 2000, 7(6): 1395-1399.
14.	段建敏, 李智, 張亮, 等. Ki-67和組織蛋白酶B在腎癌癌組織中表達的臨床意義[J]. 中華國際醫學雜志, 2003, 3(2): 99-102.

1. 　Buck MR, Karustis DG, Day NA, et al. Degradation of extracellular-matrix proteins by human cathepsin B from normal and tumour tissues[J]. Biochem J, 1992, 282(Pt 1): 273-278.
2. 　Satoh Y, Higashi T, Nouso K, et al. Cathepsin B in the growth of colorectal cancer: suppressive effect of leupeptin on the growth of DMH-induced rat colon neoplasm[J]. Acta Med Okayama, 1996，50(6): 299-303.
3. 　Shuja S, Murnane MJ. Marked increases in cathepsin B and L activities distinguish papillary carcinoma of the thyroid from normal thyroid or thyroid with non-neoplastic disease[J]. Int J Cancer, 1996, 66(4): 420-426.
4. 　Werle B, Ebert W, Klein W, et al. Cathepsin B in tumors， normal tissue and isolated cells from the human lung[J]. Anticancer Res, 1994, 14(3A): 1169-1176.
5. 　Guenette RS, Mooibroek M, Wong K, et al. Cathepsin B, a cysteine protease implicated in metastatic progression is also expressed during regression of the rat prostate and mammary glands[J]. Eur J Biochem, 1994, 226(2): 311-321.
6. 　Sloane BF, Rozhin J, Robinson D, et al. Role for cathepsin B and cystatins in tumor growth and progression[J]. Biol Chem Hoppe Seyler, 1990, 371 Suppl: 193-198.
7. 　Sivaparvathi M, McCutcheon I, Sawaya R， et al. Expression of cysteine protease inhibitors in human gliomas and meningiomas[J]. Clin Exp Metastasis, 1996, 14(4): 344-350.
8. 　Farinati F, Herszenyi L, plebani M, et al. Increased levels of cathepsin B and L, urokinase-type plasminogen activator and its inhibitor type-1 as an early event in gastric carcinogenesis[J]. Carcinogenesis, 1996, 17(12): 2581-2587.
9. 　Sinha AA, Gleason DF, Staley NA, et al. Cathepsin B in angiogenesis of hunan prostate: an immunohistochemical and immunoelectron microscopic analysis[J]. Anat Res, 1995, 241(3): 353-362.
10. 　Eiján A, Sandes EO, Riveos MD, et al. High expression of cathepsin B in trasitional bladder carcinoma correlates with tumor invasion[J]. Cancer, 2003, 98(2): 262-268.
11. 　Visscher DW, Sloane BF，Sameni M，et al.Clincopathologic significance of cathepsin B immunostaining in transitional neoplasia[J]. Mod Pathol，1994，7(1): 76-81.
12. 　Staack A， Koenig F, Daniltichenko D, et al. Cathepsin B, H and activities in urine of patients with transitional cell carcinoma of the bladder[J]. Urology, 2002, 59(2): 308-312.
13. 　Eiján AM, Sandes E, Puricelli L, et al. Cathepsin B levels in urine from bladder cancer patients[J]. Oncol Res, 2000, 7(6): 1395-1399.
14. 　段建敏, 李智, 張亮, 等. Ki-67和組織蛋白酶B在腎癌癌組織中表達的臨床意義[J]. 中華國際醫學雜志, 2003, 3(2): 99-102.

West China Medical Journal

Expression of Cathepsin B in Bladder Transitional Cell Carcinoma Tissues and Its Significance

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