雙側迷走神經切斷對肺缺血再灌注氧化應激反應的影響_《華西醫學》

作者：

江銳 , 基鵬 , 姜濤 ,  王儒蓉

四川大學華西醫院麻醉科（成都，610041）;

關鍵詞：

迷走神經肺缺血再灌注氧化應激兔

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【摘要】　目的　研究雙側迷走神經切斷對肺缺血再灌注引起的氧化應激反應的影響。　方法　將24只健康雄性新西蘭大白兔隨機分為:假手術組（S組）、缺血再灌注組（IR組）、雙側迷走神經切斷合并缺血再灌注組（NIR組）。缺血前和再灌注末抽取動脈血進行血氣分析，觀察動脈血氧分壓PaO2及肺泡動脈氧分壓差（A-aDO2）的變化。再灌注末取肺組織檢測肺的濕干重比值（W/D）和氧化應激指標，包括丙二醛（MDA）、超氧化物歧化酶（SOD）及過氧化氫酶（CAT）。　結果　與S組比較，缺血再灌注明顯降低了PaO2，增加了A-aDO2和W/D值，增加了肺組織MDA含量并降低了SOD、CAT活性；雙側迷走神經切斷進一步降低了SOD活性。　結論　切斷實驗兔的雙側迷走神經，降低了肺組織抗氧化酶-超氧化物歧化酶的活性，提示迷走神經在降低肺缺血再灌注引起的氧化應激反應中發揮了重要的調節作用。
【Abstract】　Objective　 To evaluate the effect of bilateral vagal nerves transection on lung ischemia-reperfusion induced oxidative stress.　Methods　 A total of 24 New Zealand male rabbits were randomly divided into 3 groups: sham group (S group), ischemia-reperfusion group (IR group), and bilateral vagal nerves transection with ischemia-reperfusion group (NIR group). Before ischemia and at the end of reperfusion, arterial blood samples were collected for blood gas analysis. Arterial partial pressure of oxygen (PaO2) and alveolo-arterial oxygen tension difference (A-aDO2) were detected. At the end of reperfusion, lung tissues were obtained to measure wet/dry weight ratio (W/D). Evaluation of oxidative stress indicators, including content of lung malondialdehyde (MDA), superoxide dismutase enzyme (SOD) and catalase (CAT) activities was also performed.　Results　 Compared with the S group, lung ischemia-reperfusion significantly decreased the PaO2, elevated A-aDO2 and lung W/D weight ratio. At the same time, MDA level in the lung tissue was elevated and SOD and CAT activities were decreased. After bilateral vagal nerves transection, SOD activity was further decreased.　Conclusion　 Transection of bilateral vagal nerves reduced the activity of antioxidant enzyme, especially superoxide dismutase in lung tissue, suggesting that the integrity of the vagal nerves plays an important regulatory role in ischemia-reperfusion mediated oxidative stress in the lung.

引用本文： 江銳,基鵬,姜濤,王儒蓉. 雙側迷走神經切斷對肺缺血再灌注氧化應激反應的影響. 華西醫學, 2011, 26(3): 325-327. doi: 復制

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10.	Chow MY, Goh MH, Boey SK, et al. The effects of remifentanil and thoracic epidural on oxygenation and pulmonary shunt fraction during one-lung ventilation[J]. J Cardiothorac Vasc Anesth, 2003, 17(1): 69-72.
11.	Mehdi AB, Shuman H, Fisher AB. Intracellular generation of reactive oxygen species during nonhypoxic lung ischemia[J]. Am J Physiol, 1997, 272(2 Pt 1): L294-L300.
12.	Balyasnikova IV, Visintine DJ, Gunnerson HB, et al. Propofol attenuates lung endothelial injury induced by ischemia-reperfusion and oxidative stress[J]. Anesth Analg, 2005, 100(4): 929-936.
13.	Xu JZ, Shen BZ, Li Y, et al. Edaravone attenuates ischemia-reperfusion injury by inhibiting oxidative stress in a canine lung transplantation model[J]. Chin Med J (Engl), 2008, 121(16): 1583-1587.
14.	Mizutani A, Okajima K, Murakami K, et al. Activation of sensory neurons reduces ischemia/reperfusion-induced acute renal injury in rats[J]. Anesthesiology, 2009, 110(2): 361-369.

1. 　Perrot M, Liu M, Waddell TK. Ischemia-reperfusion-induced lung injury[J]. Am J Respir Crit Care Med, 2003, 167(4): 490-511.
2. 　Ng CS, Wan S, Yim AP, et al. Pulmonary dysfunction after cardiac surgery[J]. Chest, 2002, 121(4): 1269-1277.
3. 　Naidu BV, Farivar AS, Woolley SM, et al. Novel broad-spectrum chemokine inhibitor protects against lung ischemia-reperfusion injury[J]. J Heart Lung Transplant, 2004, 23(1): 128-134.
4. 　Lefer AM, Lefer DJ. Pharmacology of the endothelium in ischemia-reperfusion and circulatory shock[J]. Annu Rev Pharmacol Toxicol, 1993, 33: 71-90.
5. 　Vainikka TL, Heikkila LJ, Kukkonen S, et al. Inhaled NO and prostacyclin during porcine single lung transplantation[J]. Ann Thorac Surg, 2001, 72(6): 1892-1897.
6. 　許健, 孫穎, 金憶, 等. 肺缺血再灌注中內皮細胞功能的研究[J]. 中國醫學雜志, 2001, 22(1): 22-23.
7. 　王洋, 王善政. ATP-MgCl2和異搏定對離體兔肺缺血再灌注保護及其機制的實驗研究[J]. 山東醫科大學學報, 2001, 39(3): 249-251.
8. 　Jurmann MJ, Dammenhayn L, Schaefers HJ, et al. Pulmonary reperfusion injury evidence for oxygen-derived free radical mediated damage and effects of different free radical scavengers[J]. Eur J Cardiothorac Surg, 1990, 4(12): 665-670.
9. 　Kawada T, Yamazaki T, Akiyama T, et al. Vagal stimulation suppresses ischemia-induced myocardial interstitial myoglobin release[J]. Life Sci, 2008, 83(13-14): 490-495.
10. 　Chow MY, Goh MH, Boey SK, et al. The effects of remifentanil and thoracic epidural on oxygenation and pulmonary shunt fraction during one-lung ventilation[J]. J Cardiothorac Vasc Anesth, 2003, 17(1): 69-72.
11. 　Mehdi AB, Shuman H, Fisher AB. Intracellular generation of reactive oxygen species during nonhypoxic lung ischemia[J]. Am J Physiol, 1997, 272(2 Pt 1): L294-L300.
12. 　Balyasnikova IV, Visintine DJ, Gunnerson HB, et al. Propofol attenuates lung endothelial injury induced by ischemia-reperfusion and oxidative stress[J]. Anesth Analg, 2005, 100(4): 929-936.
13. 　Xu JZ, Shen BZ, Li Y, et al. Edaravone attenuates ischemia-reperfusion injury by inhibiting oxidative stress in a canine lung transplantation model[J]. Chin Med J (Engl), 2008, 121(16): 1583-1587.
14. 　Mizutani A, Okajima K, Murakami K, et al. Activation of sensory neurons reduces ischemia/reperfusion-induced acute renal injury in rats[J]. Anesthesiology, 2009, 110(2): 361-369.

《華西醫學》

雙側迷走神經切斷對肺缺血再灌注氧化應激反應的影響

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《華西醫學》

雙側迷走神經切斷對肺缺血再灌注氧化應激反應的影響

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