Study on the Development of Acute Myocardial Infarction Model in Old Rats_West China Medical Journal

Authors：

LIANG Yanhong ¹ , MIAO Guobin ¹ ,  ZHANG Jian ²

1. General Department, Beijing Chaoyang Hospital, Capital University of Medical Sciences, Beijing 100020; 2.Beijing Youyi Hospital, Capital University of Medical Sciences, Beijing 100050, P. R. China;

Corresponding?author：

ZHANG Jian, Email: yi125@sina.com

Keywords：

老年大鼠; 急性心肌梗死; 血流動力學; 動物模型

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【摘要】　目的　用老年大鼠建立一種與人類發病年齡相符的穩定可重復的急性心肌梗死動物模型。　方法　 Wistar大鼠經戊巴比妥鈉麻醉后，氣管切開插管，連通呼吸機，開胸后結扎左冠狀動脈前降支。分不同時間點測血流動力學后取出心臟行病理組織學觀察。　結果　成功建立老年大鼠心肌梗死模型并進行病理組織學驗證。　結論　建立老年大鼠心梗模型是可靠的，有助于指導臨床實踐。
【Abstract】　Objective　 To develop a steady and repeatable acute myocardial infarction model in old rats in accordance to human age.　Methods　 Wistar rats were anaesthetized with sodium phenobarbital. After tracheotomy intubation, respiration machine was linked. Left anterior thoracotomy was performed to ligate the left anterior descending coronary artery. After hemodynamic parameters were obtained at various time points, hearts of the rats were taken for pathologic and histological examinations.　Results　 The model of acute myocardial infarction in old rats was established successfully and proved by pathologic and histological staining.　Conclusion　 It is reliable to establish the myocardial infarction model in old rats, which is helpful in guiding clinical practices.

Citation： LIANG Yanhong,MIAO Guobin,ZHANG Jian. Study on the Development of Acute Myocardial Infarction Model in Old Rats. West China Medical Journal, 2011, 26(7): 1025-1027. doi: Copy

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1. 　劉元生, 陳運貞. 慢性心肌梗塞大鼠實驗模型[J]. 重慶醫科大學學報, 2002, 27(2): 153-155.
2. 　劉尊齊, 崔連群, 蓋玉生. 大鼠急性心肌梗死模型的制備[J]. 基礎醫學與臨床, 2007, 7(9): 34-35.
3. 　趙靜, 邵素霞, 張雷, 等. 改良法制備大鼠心肌梗死模型[J]. 河北醫科大學學報, 2010, 31(1): 1-4.
4. 　Liu L, Azhar G, Gao W, et al. Bcl-2 and Bax expression in adult rat hearts after coronary occlusion: age-associated differences[J]. Am J Physiol, 1998, 275(1): 315-322.
5. 　Azhar G, Gao W, Liu LX, et al. Ischemia-reperfusion in the adult mouse heart:influence of age[J]. Exp Gerontol, 1999, 286(34): 699-714.
6. 　Kajstura J, Cheng W, Sarangarajan R, et al. Necrotic and apoptotic myocyte cell death in the aging heart of Fischer 344 rats[J]. Am J Physiol Heart Circ Physiol, 1996, 271(5): 1215-1228.
7. 　Bhind R, Witting PK, Mcahon AC, et al. Rat models of myocardial infarction. Pathogenetic insights and clinical relevance[J]. Thromb Haemost, 2006, 96(5): 602-610.
8. 　Tarnavski O, McMullen JR, Schinke M, et al. Mouse cardiac surgery: comprehensive techniques for the generation of mouse models of human diseases and their application for genomic studies[J]. Physiol Genomics, 2004, (16): 349-360.
9. 　李保, 馬業新, 解軍, 等. 大鼠心肌梗死模型制備的改進[J]. 山西醫科大學學報, 2005, 36(1): 75-78.
10. 　王飛燕, 李學文, 仝凌. 影響大鼠心肌梗死模氆存活率的因素分析[J]. 中西醫結合心腦血管病雜志, 2008, 6(7): 862-863.
11. 　Ramunddal T, Lorentzon M, Omerovie E. Decreased mortality in a rat model of acute postinfarction heart failure[J]. Biochem Biophys Res Commun, 2006, 341(2): 459-463.
12. 　Kajstura J, Cheng W, Reiss K, et al. Apoptotic and necrotic myocyte cell death are independent contributing variables of infarct size in rats[J]. Laboratory Investigation, 1996, 74(1): 86-107.

West China Medical Journal

Study on the Development of Acute Myocardial Infarction Model in Old Rats

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